Error: cannot take a sample larger than the population when 'replace = FALSE'

[CalmRealistic]

Hi, I have no idea what I am doing wrong. Yesterday i managed to go all the way into umap, plots and averythign.

Today I changed sub.targets from 20 000 to 30 000 and ran flowsom with 20 metaclusters.

After that, when i try to do umap or tSNE I get an error with sample size. Error: cannot take a sample larger than the population when 'replace = FALSE'

I checked and my response group has
Var1 Freq
1 CR 12288987
2 NR 14861742

Thank you for your help

``{r nb cells to take}
sub.targets <- c(30000, 30000)
sub.targets

```{r Flowsom}
cell.dat <- run.flowsom(cell.dat, cluster.cols, meta.k = 20)
       fwrite(cell.dat, "clustered.data.csv")

cell.sub <- do.subsample(cell.dat, sub.targets, response.col)
    cell.sub <- run.umap(cell.sub, cluster.cols)
 
    fwrite(cell.sub, "clustered.dataumap20.DR.csv")