diff --git a/Notebook_Maryam/methods.md b/Notebook_Maryam/methods.md index d464c99..e18a443 100644 --- a/Notebook_Maryam/methods.md +++ b/Notebook_Maryam/methods.md @@ -20,8 +20,8 @@ We used Flye (v.2.8.2-b1691) for de novo assembly of each of the strain's genome ## Comparing genomes using dotplot -<<<<<<< HEAD + We used [re-DOT-able](https://www.bioinformatics.babraham.ac.uk/projects/redotable/) to compare the reference genome and assembled genome for each strain. ======= In order to visualize pairwise comparison between sequences we used a desktop app ([re-DOT-able](https://www.bioinformatics.babraham.ac.uk/projects/redotable/) ) from Babraham Bioinformatics to plot interactively and also save the plots as images. We made pairwise comparisons between the reference genome and each of the seven assembled genomes to look for insert location and for any genome re-arrangements. In order to check the location Additionally we also plotted each assembly against the relevant insert sequences. For detailed information; Simon Andrews, the developer has a really good [video tutorial](https://www.youtube.com/watch?v=qPxl2hflG9Q&feature=emb_logo). ->>>>>>> ab3610188d05a605c52c9c339bb3b3433392eccd +