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03_Results.md

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Results

Assembly

BUSCOs

Insert Complete BUSCOs (C) Complete and single-copy BUSCOs (S) Complete and duplicated BUSCOs (D) Fragmented BUSCOs (F) Missing BUSCOs (M) Total BUSCO group searched
6 687 (90.7%) 685 (90.4%) 2 (0.3%) 6 (0.8%) 65 (8.5%) 758
53 681 (89.8%) 680 (89.7%) 1 (0.1%) 7 (0.9%) 70 (9.3%) 758
59 685 (90.3%) 681 (89.8%) 4 (0.5%) 7 (0.9%) 66 (8.8%) 758
88 678 (89.4%) 677 (89.3%) 1 (0.1%) 6 (0.8%) 74 (9.8%) 758

Assembly stats

insert # of scaffolds Total size of scaffolds Longest scaffold shortest scaffold Number of scaffolds > 1M nt Number of scaffolds > 100K nt Number of scaffolds > 10K nt N50 L50 N90 L90
6 11 16022515 3579715 16004 8 10 11 1894960 3 1111997 7
53 10 15787988 4587006 4261 6 7 8 3523743 2 1113568 6
59 12 15994518 3515593 60343 7 9 12 1894562 3 1112789 7
88 23 15767318 3532437 2149 7 9 13 1755085 3 1109753 7

Rearrangement

We did not observe rearrangement in the assembled strains: dotplots in eps format

dotplots

Insert locations

strain insert insert location 2019 ref genome number of inserts insert location on 2007 genome chromosome
6 (b13) 1.BSA4upstream PQNB01000001.1 778685 1 NC_009042.1 1982553 Chromosome 2
53 (b16) 1.BSA4upstream PQNB01000001.1 778195 1 NC_009042.1 1963057 Chromosome 2
55 (b18) 1.BSA4upstream PQNB01000001.1 778685 1 NC_009042.1 1982553 Chromosome 2
88 (b19 ) 1.BSA4upstream PQNB01000001.1 778195 1 NC_009042.1 1963058 Chromosome 2
6 (b13) 2.GTDNUdownstream PQNB01000023.1 107903 2 (different strands) NC_009045.1 564770 chromosome 5
53 (b16) 2.GTDNUdownstream PQNB01000019.1 593474 1 NC_009068.1 1580399 chromosome 1
55 (b18) 2.GTDNUdownstream PQNB01000022.1 615582 2 (different strands) NC_009044.1 891874 chromose 4
88 (b19) 2.GTDNUdownstream PQNB01000028.1 440888 2 (same strand) NC_009068.1 3058614 chromosome 1

Insert 2 locations and number of insertions based on the dotplot

b13-vs-insert2

b16-vs-insert2

b18-vs-insert2

b19-vs-insert2