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nextflow_schema.json
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{
"$schema": "http://json-schema.org/draft-07/schema",
"$id": "https://raw.githubusercontent.com/IARCbioinfo/RNAseq-fusion-nf/master/nextflow_schema.json",
"title": "IARCbioinfo RNAseq-fusion-nf pipeline parameters",
"description": "This pipeline performs fusion-gene discovery from junction reads identified by STAR during alignment.",
"type": "object",
"definitions": {
"input_output_params": {
"title": "Input_output_params",
"type": "object",
"description": "Input and output parameters",
"default": "",
"properties": {
"input_folder": {
"type": "string",
"description": "Folder containing fastq files and STAR junction files",
"default": "."
},
"input_file": {
"type": "string",
"description": "Input file (comma-separated) with 4 columns: SM(sample name), pair1 (path to fastq pair 1), pair2 (path to fastq pair 2), and junction (path to junction file)"
},
"fastq_ext": {
"type": "string",
"description": "Extension of fastq files",
"default": "fq.gz"
},
"suffix1": {
"type": "string",
"description": "Suffix of 1st element of fastq files pair",
"default": "_1"
},
"suffix2": {
"type": "string",
"description": "Suffix of 2nd element of fastq files pair",
"default": "_2"
},
"junction_suffix": {
"type": "string",
"description": "Suffix of STAR chimeric junction files",
"default": "Chimeric.SJ.out.junction"
},
"output_folder": {
"type": "string",
"description": "Output folder",
"default": "results_fusion"
}
}
},
"computing_params": {
"title": "Computing_params",
"type": "object",
"description": "Computing parameters",
"default": "",
"properties": {
"cpu": {
"type": "integer",
"description": "Number of cpu used by bwa mem and sambamba",
"default": 2
},
"mem": {
"type": "string",
"description": "Size of memory used for mapping (in GB)",
"default": "2"
}
}
},
"ref_params": {
"title": "Ref_params",
"type": "object",
"description": "Reference parameters",
"default": "",
"properties": {
"CTAT_folder": {
"type": "string",
"description": "Folder with STAR-Fusion bundle (CTAT)",
"default": "."
},
"starfusion_path": {
"type": "string",
"description": "Path to STAR-fusion executable; note that the default is set to the location in the singularity container",
"default": "/usr/local/src/STAR-Fusion/STAR-Fusion"
}
}
},
"pipeline_params": {
"title": "Pipeline_params",
"type": "object",
"description": "Pipeline parameters",
"default": "",
"properties": {
"junctions": {
"type": "boolean",
"description": "Option to use STAR junction files already generated"
},
"help": {
"type": "boolean",
"description": "Display help"
}
}
}
},
"allOf": [
{
"$ref": "#/definitions/input_output_params"
},
{
"$ref": "#/definitions/computing_params"
},
{
"$ref": "#/definitions/ref_params"
},
{
"$ref": "#/definitions/pipeline_params"
}
]
}