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addReproduciblePeakSet error #2265

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superSXB opened this issue Feb 19, 2025 · 1 comment
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addReproduciblePeakSet error #2265

superSXB opened this issue Feb 19, 2025 · 1 comment
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@superSXB
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Hello, recently when I was using ArchR and reached the step of "proj_sub <- addReproduciblePeakSet(ArchRProj = proj, groupBy = 'celltype', genomeSize = 2.78e9)", I encountered a situation where the summit.bed file was not successfully generated. This file should be generated by masc2 when it reads the insertions.bed file. However, the summit.bed file was not successfully generated, and ArchR continued to execute, resulting in an error. I have now determined that my macs2 should be fine because I can successfully run other projects. I also tried reducing the number of threads in "addArchRThreads(threads = 20)", but it still didn't work. I am conducting the analysis on a large computing node, and I'm sure that there is sufficient memory. The same steps work for my other projects, but occasionally I encounter this error. After encountering this error, I can only import the peakset generated by cellranger, which I think is not very accurate. So I would like to ask if you have any solutions. I'm eager to know the solution. I would be extremely grateful if you could reply.

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@superSXB superSXB added the bug Something isn't working label Feb 19, 2025
@immanuelazn
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Hi @superSXB! Thanks for using ArchR! Please make sure that your post belongs in the Issues section. Only bugs and error reports belong in the Issues section. Usage questions and feature requests should be posted in the Discussions section, not in Issues.

If you are getting an error, it is likely due to something specific to your dataset, usage, or computational environment, all of which are extremely challenging to troubleshoot. As such, we require reproducible examples (preferably using the tutorial dataset) from users who want assistance. If you cannot reproduce your error, we will not be able to help.
Before going through the work of making a reproducible example, search the previous Issues, Discussions, function definitions, or the ArchR manual and you will likely find the answers you are looking for.
If your post does not contain a reproducible example, it is unlikely to receive a response.

In addition to a reproducible example, you must do the following things before we help you, unless your original post already contained this information:
1. If you've encountered an error, have you already searched previous Issues to make sure that this hasn't already been solved?
2. Did you post your log file? If not, add it now.
3. Remove any screenshots that contain text and instead copy and paste the text using markdown's codeblock syntax (three consecutive backticks). You can do this by editing your original post.

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