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Hi.
I'm trying to run FlaGs using local genomes, and I have some trouble creating the files.
I was reading the paper and I have a straight forward question. Is it possible to create our own database with sequences not available in NCBI (by generating the gff3, and the fasta files),a nd also use as input sequences that are not in NCBI (don't have an NCBI accession number)? Someone have done this?
I try, and fail.
This is the error that I am receiving: The submitted query might include characters not found in NCBI protein accessions eg. > , # , ! etc. Please provide correct format, Thanks!
Here is the example of one of the fasta files created with my personal sequences:
Hi.
I'm trying to run FlaGs using local genomes, and I have some trouble creating the files.
I was reading the paper and I have a straight forward question. Is it possible to create our own database with sequences not available in NCBI (by generating the gff3, and the fasta files),a nd also use as input sequences that are not in NCBI (don't have an NCBI accession number)? Someone have done this?
I try, and fail.
This is the error that I am receiving:
The submitted query might include characters not found in NCBI protein accessions eg. > , # , ! etc. Please provide correct format, Thanks!
Here is the example of one of the fasta files created with my personal sequences:
And one example of the gff file edit from the Prodigal output:
Thank you for your help.
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