diff --git a/samples/subsys/testsuite/pytest/shell/testcase.yaml b/samples/subsys/testsuite/pytest/shell/testcase.yaml
index 86b9314f4444..f256cd9df8f5 100644
--- a/samples/subsys/testsuite/pytest/shell/testcase.yaml
+++ b/samples/subsys/testsuite/pytest/shell/testcase.yaml
@@ -12,3 +12,17 @@ tests:
       - test_framework
       - pytest
       - shell
+  sample.pytest.shell.vt100_colors_off:
+    filter: CONFIG_SERIAL and dt_chosen_enabled("zephyr,shell-uart")
+    min_ram: 40
+    harness: pytest
+    extra_configs:
+      - arch:posix:CONFIG_NATIVE_UART_0_ON_STDINOUT=y
+      - CONFIG_SHELL_VT100_COLORS=n
+    integration_platforms:
+      - native_sim
+      - qemu_cortex_m3
+    tags:
+      - test_framework
+      - pytest
+      - shell
diff --git a/scripts/pylib/pytest-twister-harness/src/twister_harness/device/device_adapter.py b/scripts/pylib/pytest-twister-harness/src/twister_harness/device/device_adapter.py
index fd97972c74af..87ecac0f75a9 100644
--- a/scripts/pylib/pytest-twister-harness/src/twister_harness/device/device_adapter.py
+++ b/scripts/pylib/pytest-twister-harness/src/twister_harness/device/device_adapter.py
@@ -240,7 +240,7 @@ def _handle_device_output(self) -> None:
         with open(self.handler_log_path, 'a+') as log_file:
             while self.is_device_running():
                 if self.is_device_connected():
-                    output = self._read_device_output().decode(errors='replace').strip()
+                    output = self._read_device_output().decode(errors='replace').rstrip("\r\n")
                     if output:
                         self._device_read_queue.put(output)
                         log_file.write(f'{output}\n')