diff --git a/README.md b/README.md
index 88f19d4..c913851 100644
--- a/README.md
+++ b/README.md
@@ -4,7 +4,8 @@
 <a href="https://hits.seeyoufarm.com"><img src="https://hits.seeyoufarm.com/api/count/incr/badge.svg?url=https%3A%2F%2Fgithub.com%2FCrazzy-Rabbit%2FScript-in-Bio&count_bg=%2379C83D&title_bg=%23555555&icon=microgenetics.svg&icon_color=%23E7E7E7&title=%E8%AE%BF%E9%97%AE%E9%87%8F&edge_flat=false"/></a>
 
 - [ ] pangenome --->  want to learn and do in the future
-- [ ] 单细胞分析 --->  want to learn and do in the future
+- [ ] [单细胞分析](https://github.com/Crazzy-Rabbit/Script-in-Bio/tree/main/%E5%8D%95%E7%BB%86%E8%83%9ERNA-Seq%E5%88%86%E6%9E%90) --->  want to learn and do in the future
+      - [X] 用的Seurat4，从前期处理到UMAP降维的记录
 - [ ] 孟德尔随机化 --->  want to learn and do in the future
     - [ ] 整合多组学--SMR分析，精确定位致病突变
 - [ ] 算法 + 数据结构 --->  want to learn and do in the future