diff --git a/05-control_calidad.R b/05-control_calidad.R index dbec4c5..ea05b3a 100644 --- a/05-control_calidad.R +++ b/05-control_calidad.R @@ -322,12 +322,12 @@ col = "grey80" ## ----pbmc_chrMT_ayuda------------------------------------------------------------------------------- sce.pbmc <- sce.pbmc[, which(e.out$FDR <= 0.001)] is.mito <- grep("^MT-", rowData(sce.pbmc)$Symbol) -sce.pmbc <- addPerCellQC(sce.pbmc, subsets = list(MT = is.mito)) +sce.pbmc <- addPerCellQC(sce.pbmc, subsets = list(MT = is.mito)) discard.mito <- - isOutlier(sce.pmbc$subsets_MT_percent, type = "higher") + isOutlier(sce.pbmc$subsets_MT_percent, type = "higher") plot( - sce.pmbc$sum, - sce.pmbc$subsets_MT_percent, + sce.pbmc$sum, + sce.pbmc$subsets_MT_percent, log = "x", xlab = "Total count", ylab = "Mitochondrial %"