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Simulated 3'tagged single cell dataset #26

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Franzx7 opened this issue Nov 4, 2022 · 0 comments
Open

Simulated 3'tagged single cell dataset #26

Franzx7 opened this issue Nov 4, 2022 · 0 comments

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@Franzx7
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Franzx7 commented Nov 4, 2022

Dear All,

Thanks first for this very useful tool.

I have a issue with the simulation of single cell dataset using 3'tag.
I observe that whatever parameter I used in the minnow simulate command (even the option --polyA), I simulate reads around a fixed distance from transcripts end of 400 bp.
How should I process to simulate reads to a closer distance from the end ? (10bp, 50bp or 100/150bp)
This will be very useful in order to get a simulated dataset with reads position reflecting the reads position of my inital experimental 3'tag single cell dataset.

Thanks in advance
Franz

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