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I have a issue with the simulation of single cell dataset using 3'tag.
I observe that whatever parameter I used in the minnow simulate command (even the option --polyA), I simulate reads around a fixed distance from transcripts end of 400 bp.
How should I process to simulate reads to a closer distance from the end ? (10bp, 50bp or 100/150bp)
This will be very useful in order to get a simulated dataset with reads position reflecting the reads position of my inital experimental 3'tag single cell dataset.
Thanks in advance
Franz
The text was updated successfully, but these errors were encountered:
Dear All,
Thanks first for this very useful tool.
I have a issue with the simulation of single cell dataset using 3'tag.
I observe that whatever parameter I used in the minnow simulate command (even the option --polyA), I simulate reads around a fixed distance from transcripts end of 400 bp.
How should I process to simulate reads to a closer distance from the end ? (10bp, 50bp or 100/150bp)
This will be very useful in order to get a simulated dataset with reads position reflecting the reads position of my inital experimental 3'tag single cell dataset.
Thanks in advance
Franz
The text was updated successfully, but these errors were encountered: