\")), \" with each name separated by a semi-colon. Middle names can be excluded. \",\n \"Your list of names should look something like this: \",\n ),\n ui.code(ui.strong(\"John Doe; Jane Doe; John Quincy Adams; Franklin Delano Roosevelt\")),\n ui.p(ui.strong(\"Note: \"), \"SeqSender uses a natural language processing library to sort names into first, middle, and last. \",\n \"While SeqSender does handle international and hyphenated names fairly well, it can still have issues correctly identifying every name's structure. \",\n \"If you find this is an issue for your name, try either removing/initializing your middle name or by removing the hypen from your name if present. \",\n ),\n ),\n ui.tags.li(ui.strong(\"collection_date\")),\n ui.tags.ul(\n ui.p(\"This should be an ISO-8601 formatted collection date for your sample. \",\n \"The format's accepted are: \", ui.code(ui.strong(\"YYYY-MM-DD\")), \", \", ui.code(ui.strong(\"YYYY-MM\")), \", or \", ui.code(ui.strong(\"YYYY\")), \". \",\n \"Time should not be included as it could create issues during submission. \"\n ),\n ),\n ui.tags.li(ui.strong(\"bioproject\")),\n ui.tags.ul(\n ui.p(\"This field is required when submitting to SRA or BioSample but is recommended to be used for all NCBI database submissions. \"\n \"Visit the \", ui.a(ui.strong(\"BioProject Web Portal\"), href=\"https://submit.ncbi.nlm.nih.gov/subs/bioproject/\"), \" to create a BioProject for your samples. \",\n ),\n ui.p(\"To add your BioProject to your metadata, all you need is the accession ID \", ui.code(ui.strong(\"(PRJNAxxxxxx, i.e. PRJNA123456)\")), \" created once you complete the BioProject.\"),\n ),\n ),\n]\n\nsra_database_submission = [\n ui.h4(\"Raw Reads Locations\"),\n ui.p(\"The raw reads for your samples can be stored in a variety of different locations and still be used in submission with SeqSender. \",\n \"Raw reads can either be stored locally or in the cloud when uploading to SRA, as well as, multiple files can be associated to one sample. \",\n \"The metadata column \", ui.code(ui.strong(\"sra-file_#\")), \", can be repeated where # is the numeric value of that file. \",\n \"(i.e. If you were uploading two files, you would have the first file path in \", ui.code(\"sra-file_1\"), \" and the second file path in \", ui.code(\"sra-file_2\"), \".)\"\n ),\n ui.p(\"If your raw reads are stored in the cloud you will want to include the full URL path to the file for the metadata column. \",\n \"To signify to SeqSender that this is a cloud URL, in the metadata column \", ui.code(ui.strong(\"sra-file_location\")), \", the keyword \", ui.code(ui.strong(\"cloud\")), \" should be included. \",\n ),\n ui.p(\"If your raw reads are locally stored locally they can be located in two locations to be accessed by SeqSender. \",\n \"If just the file name is included in your metadata column, then your raw reads should be stored in a folder named \", ui.code(ui.strong(\"raw_reads\")), \" at the location of your \", ui.code(ui.strong(\"--submission_dir\")), \" when using SeqSender. \",\n \"Otherwise, the full file path can be included in your submission. (\", ui.strong(\"Note: \"), \"Be sure if using docker/singularity that SeqSender has access to the location of your raw reads to prevent submission errors. \",\n \"For local files, the metadata column \", ui.code(ui.strong(\"sra-file_location\")), \" should use the keyword \", ui.code(ui.strong(\"local\")), \" to signify that the file is stored locally. \",\n ),\n ui.h4(\"Raw Reads Processing Error\"),\n ui.p(\"If your raw reads fail to process on SRA and it could be because they are incorrectly formatted for SRA. \",\n \"If the SRA team notifies you of this and recommends adding a \", ui.strong(\"sra loader\"), \" to correct the problem, that can be easily added to SeqSender. \",\n \"Using the metadata column\", ui.code(ui.strong(\"sra-loader\")), \" add the name of the specified loader by the SRA team to correct this issue. \",\n ),\n ui.h4(\"Metadata Fields\"),\n ui.p(\"For all required fields for SeqSender to submit data to SRA, refer to the tab: \", ui.strong(\"Submission Wizard\"), \". \",\n \"For all other optional fields, refer to NCBI's \", ui.a(ui.strong(\"SRA portal\"), href=\"https://www.ncbi.nlm.nih.gov/sra/docs/submitportal/\"), \", any metadata field can be easily added by using the specified column name with the prefix \", ui.code(\"sra-\"), \". \",\n ),\n]\n\nbiosample_database_submission = [\n ui.h4(\"Metadata Fields\"),\n ui.p(\"Required metadata columns change based on the specified \", ui.a(ui.strong(\"BioSample Package\"), href=\"\"), \" used in your submission. \",\n \"Refer to the tab: \", ui.strong(\"Submission Wizard\"), \" to get all the required metadata columns for your chosen package. \",\n \"If you want to add a supported column that is not present in the submission wizard, you can easily add it to your metadata using the specified column name with the prefix \", ui.code(\"bs-\"), \". \",\n ),\n]\n\nfirst_submission_body = [\n ui.h2(\"My First Submission\"),\n ui.hr(),\n ui.navset_pill_list(\n ui.nav_panel(\"Generate Test Data\", test_data),\n ui.nav_panel(\"SeqSender Requirements\", seqsender_requirements),\n ui.nav_menu(\"Database Specific Requirements\",\n ui.nav_panel(\"SRA\", sra_database_submission),\n ui.nav_panel(\"BioSample\", biosample_database_submission),\n ),\n id=\"tab\",\n ),\n]\n", "type": "text"}, {"name": "prerequisites.py", "content": "from shiny import App, render, ui\n\nncbi_prereq = [\n ui.h4(\"NCBI Submissions:\"),\n ui.p(\"SeqSender utilizes a UI-Less Data Submission Protocol to bulk upload submission files to NCBI databases. SeqSender also uses \", ui.a(ui.strong(\"table2asn\"), href=\"https://www.ncbi.nlm.nih.gov/genbank/table2asn/\"), \", to upload GenBank submissions for organisms other than Influenza and COVID-19, by emailing it to NCBI via a Simple Mail Transfer Protocol (SMTP). \",\n \"To begin submitting to NCBI with SeqSender, you must: \",\n ),\n ui.tags.ul(\n ui.tags.li(\"Have a \", ui.a(ui.strong(\"NCBI account\"), href=\"https://account.ncbi.nlm.nih.gov/\"), \". Sign up or login in to continue. \"),\n ui.p(\"To generate the files for submitting data to NCBI via their \", ui.a(ui.strong(\"submission portal\"), href = \"https://submit.ncbi.nlm.nih.gov/\"), \" or if you're only submitting data via table2asn, no extra steps must be taken. Use the tab: \", ui.strong(\"Submission Wizard\"), \" to get the required metadata columns for the database you're uploading to. \",\n \"If you're wanting SeqSender to handle uploading your samples for you, then follow the next steps: \",\n ),\n ui.tags.li(\"Contact NCBI at: \", ui.code(ui.strong(\"gb-admin@ncbi.nlm.nih.gov\")), \" to create your institution/group/lab's UI-less submission account. This will create an account for your institution/group/lab's and allow you to specify NCBI users of your institution/group/lab's access to the uploads via NCBI's \", ui.a(ui.strong(\"web portal\"), href=\"https://submit.ncbi.nlm.nih.gov/subs/\"), \". Make sure to answer the following questions below when contacting NCBI: \"),\n ui.tags.ul(\n ui.tags.li(ui.strong(\"MyNCBI account email of the primary submitter\")),\n ui.tags.li(ui.strong(\"center/account abbreviation\")),\n ui.tags.li(ui.strong(\"full center/account name\")),\n ui.tags.li(ui.strong(\"names and email addresses of all additional users\")),\n ui.tags.li(ui.strong(\"postal address of institute (including postal code and country)\")),\n ),\n ui.tags.li(\"Once created, the UI-less submission account credentials and spuid_namespace can be added to your config file to begin submitting your samples to NCBI. Be sure to use the SeqSender submission flag \", ui.code(ui.strong(\"--test\")), \" to perform an initial test submission to NCBI, to ensure everything is setup correctly. \"),\n )\n]\ngisaid_prereq = [\n ui.h4(\"GISAID Submissions\"),\n ui.p(\"SeqSender makes use of GISAID's Command Line Interface tools to bulk upload sample organism's metadata and assembly sequence to the GISAID databases. Currently the following databases are supported for GISAID: EpiFLU, EpiCoV, EpiArbo, EpiRSV. \",\n \"To begin submitting data to GISAID using SeqSender, you must: \",\n ),\n ui.tags.ul(\n ui.tags.li(\n ui.p(\"Have a GISAID account and access to the Epi database you're wanting to upload to. \",\n ui.a(ui.strong(\"Sign up\"), href=\"https://gisaid.org/register/\"), \" for a GISAID account if you do not have one and be sure you can access the database on your \", ui.a(ui.strong(\"submission portal\"), href=\"https://www.epicov.org/epi3/frontend#\"), \" to ensure you have met the data access agreements for GISAID. \",\n ),\n ),\n ui.p(\"To generate the files for submitting data to GISAID via their \", ui.a(ui.strong(\"submission portal\"), href = \"https://www.epicov.org/epi3/frontend#\"), \", no extra steps must be taken. Use the tab: \", ui.strong(\"Submission Wizard\"), \" to get the required metadata columns for the database you're uploading to. \",\n \"If you're wanting SeqSender to handle uploading your sequences for you, then follow the next steps: \",\n ),\n ui.tags.li(\n ui.p(\"Request a \", ui.strong(\"Client-ID\"), \" from GISAID for the database you're wanting to upload to by emailing them at: \", ui.code(ui.strong(\"clisupport@gisaid.org\")), \". \"),\n ),\n ui.tags.li(\n ui.p(\"Download the CLI tool for your chosen database from GISAID's \", ui.a(ui.strong(\"submission portal\"), href=\"https://www.epicov.org/epi3/frontend#\"), \" and place the \", ui.strong(\"CLI File\"), \" in one of two locations to be accessed by SeqSender: \"),\n ui.tags.ul(\n ui.tags.li(\"Place it in SeqSender's local directory in an a folder called: \", ui.code(ui.strong(\"seqsender/gisaid_cli/\")), \". i.e.(seqsender/gisaid_cli/epiFLU) or (seqsender/gisaid_cli/epiPOX/epiPOX)\"),\n ui.tags.li(\"Place it in the directory \", ui.code(ui.strong(\"--submission_dir\")), \" when running SeqSender in a folder called: \", ui.code(ui.strong(\"<--submission_dir>/gisaid_cli/\")), \". i.e.(my_folder/gisaid_cli/epiCOV) or (my_folder/gisaid_cli/epiFLU/EpiFLU)\")\n ),\n ),\n ui.tags.li(\n ui.p(\"Make a test submission first with the \", ui.strong(\"test Client-ID\"), \" provided by GISAID when you requested your client ID. Use the tab: \", ui.strong(\"Submission Wizard\"), \" to input your client-ID and login credentials for GISAID to upload your sequences via their CLI tool. \"),\n ),\n ),\n]\nprerequisites_body = [\n ui.h2(\"Prerequisites\"),\n ui.navset_tab(\n ui.nav_panel(\"NCBI\", ncbi_prereq),\n ui.nav_panel(\"GISAID\", gisaid_prereq),\n id=\"prerequisites_tab\",\n ),\n]\n", "type": "text"}, {"name": "seqsender.css", "content": ".custom_sidebar, .accordion-title, h1, h2, h3, h4, h5, h6 {\n font-weight: 600;\n background-image: radial-gradient(circle, #023382, #0359e2);\n color: transparent;\n background-clip: text;\n -webkit-background-clip: text;\n}\nhr {\n border: 2px solid black;\n}\n.custom_header {\n border-bottom: 2px solid DimGray;\n padding-bottom: 2px;\n}\n.custom_sidebar {\n text-decoration: none;\n font-size: large;\n padding: 5px;\n}\n.terminal_css {}\n", "type": "text"}, {"name": "seqsender_config.yaml", "content": "Submission:\n NCBI:\n Password: mypassword\n Username: myusername\n", "type": "text"}, {"name": "setup.py", "content": "from shiny import App, Inputs, Outputs, Session, render, req, ui, reactive\nfrom htmltools import TagList, div\nimport shiny_tools\n####################### SETUP PAGE ###############################\nyaml_css = \"background-color: #F0F0F0;white-space: nowrap; font-size: 20px ;margin-top:-15px;font-family: Consolas,Monaco,Lucida Console,Liberation Mono,DejaVu Sans Mono,Bitstream Vera Sans Mono,Courier New, monospace;-webkit-user-select: none; -ms-user-select: none; user-select: none;\"\n\ndef config_indent(tabs, string, custom_style = \"display:inline-block;\"):\n return div(ui.HTML((\" | \" * tabs) + string), style=custom_style)\n\ndef config_text_input(id, placeholder, help_msg, custom_style = \"display:inline-block;height:5px;\"):\n return div(ui.tooltip(ui.input_text(id, label=None, placeholder=placeholder), help_msg, id=(id + \"_tooltip\")), style=custom_style)\n\nsetup_body = [\n ui.h2(\"Setup\"),\n ui.layout_columns(\n ui.card(\n ui.card_header(\n ui.h4(\"Select Database:\", style=\"display:inline-block;\"),\n shiny_tools.create_help_tooltip(\n id = \"db_select_tooltip\",\n description = \"Select all databases you plan to submit your data to. The databases you plan to submit to will change the requirements for the config and metadata file.\",\n position=\"right\",\n ),\n ),\n ui.input_checkbox(\"BioSample_checkbox\", \"BioSample\", width=None),\n ui.tooltip(ui.input_checkbox(\"SRA_checkbox\", \"SRA\", width=None),\n \"When submitting to SRA, a BioSample submission is also required.\",\n style=\"display:inline-block;float:right;\"),\n ui.input_checkbox(\"GenBank_checkbox\", \"GenBank\", width=None),\n ui.input_checkbox(\"GISAID_checkbox\", \"GISAID\", width=None),\n ),\n ui.card(\n ui.card_header(\n ui.h3(\"Database Options:\", style=\"display:inline-block;\"),\n shiny_tools.create_help_tooltip(id = \"db_option_tooltip\",\n description = \"Select database dependent options. This changes metadata requirements based on database submission criteria.\",\n position = \"right\",\n ),\n ),\n # If BioSample checkbox checked then load BioSample Package Options\n ui.panel_conditional(\n \"input.BioSample_checkbox\",\n ui.input_select(\n \"BioSample_packages\",\n label=\"Select BioSample Package:\",\n choices={\"STANDARD\": {\"SARS-CoV-2.cl.1.0\":\"SARS-CoV-2.cl.1.0\",\"SARS-CoV-2.wwsurv.1.0\":\"SARS-CoV-2.wwsurv.1.0\",\"Virus.1.0\":\"Virus.1.0\",\"Beta-lactamase.1.0\":\"Beta-lactamase.1.0\",\"Human.1.0\":\"Human.1.0\",\"Invertebrate.1.0\":\"Invertebrate.1.0\",\"Metagenome.environmental.1.0\":\"Metagenome.environmental.1.0\",\"Microbe.1.0\":\"Microbe.1.0\",\"Model.organism.animal.1.0\":\"Model.organism.animal.1.0\",\"OneHealthEnteric.1.0\":\"OneHealthEnteric.1.0\",\"Plant.1.0\":\"Plant.1.0\"},\n \"PATHOGEN\": {\"Pathogen.cl.1.0\":\"Pathogen.cl.1.0\",\"Pathogen.env.1.0\":\"Pathogen.env.1.0\"},\n \"MIGS.ba\": {\"MIGS.ba.6.0\":\"MIGS.ba.6.0\",\"MIGS.ba.agriculture.6.0\":\"MIGS.ba.agriculture.6.0\",\"MIGS.ba.air.6.0\":\"MIGS.ba.air.6.0\",\"MIGS.ba.built.6.0\":\"MIGS.ba.built.6.0\",\"MIGS.ba.food-animal.6.0\":\"MIGS.ba.food-animal.6.0\",\"MIGS.ba.food-farm.env.6.0\":\"MIGS.ba.food-farm.env.6.0\",\"MIGS.ba.food-human.foods.6.0\":\"MIGS.ba.food-human.foods.6.0\",\"MIGS.ba.food-prod.facility.6.0\":\"MIGS.ba.food-prod.facility.6.0\",\"MIGS.ba.host-associated.6.0\":\"MIGS.ba.host-associated.6.0\",\"MIGS.ba.human-associated.6.0\":\"MIGS.ba.human-associated.6.0\",\"MIGS.ba.human-gut.6.0\":\"MIGS.ba.human-gut.6.0\",\"MIGS.ba.human-oral.6.0\":\"MIGS.ba.human-oral.6.0\",\"MIGS.ba.human-skin.6.0\":\"MIGS.ba.human-skin.6.0\",\"MIGS.ba.human-vaginal.6.0\":\"MIGS.ba.human-vaginal.6.0\",\"MIGS.ba.hydrocarbon-cores.6.0\":\"MIGS.ba.hydrocarbon-cores.6.0\",\"MIGS.ba.hydrocarbon-fluids.swabs.6.0\":\"MIGS.ba.hydrocarbon-fluids.swabs.6.0\",\"MIGS.ba.microbial.6.0\":\"MIGS.ba.microbial.6.0\",\"MIGS.ba.miscellaneous.6.0\":\"MIGS.ba.miscellaneous.6.0\",\"MIGS.ba.plant-associated.6.0\":\"MIGS.ba.plant-associated.6.0\",\"MIGS.ba.sediment.6.0\":\"MIGS.ba.sediment.6.0\",\"MIGS.ba.soil.6.0\":\"MIGS.ba.soil.6.0\",\"MIGS.ba.symbiont-associated.6.0\":\"MIGS.ba.symbiont-associated.6.0\",\"MIGS.ba.wastewater.6.0\":\"MIGS.ba.wastewater.6.0\",\"MIGS.ba.water.6.0\":\"MIGS.ba.water.6.0\"},\n \"MIGS.eu\": {\"MIGS.eu.6.0\":\"MIGS.eu.6.0\",\"MIGS.eu.agriculture.6.0\":\"MIGS.eu.agriculture.6.0\",\"MIGS.eu.air.6.0\":\"MIGS.eu.air.6.0\",\"MIGS.eu.built.6.0\":\"MIGS.eu.built.6.0\",\"MIGS.eu.food-animal.6.0\":\"MIGS.eu.food-animal.6.0\",\"MIGS.eu.food-farm.env.6.0\":\"MIGS.eu.food-farm.env.6.0\",\"MIGS.eu.food-human.foods.6.0\":\"MIGS.eu.food-human.foods.6.0\",\"MIGS.eu.food-prod.facility.6.0\":\"MIGS.eu.food-prod.facility.6.0\",\"MIGS.eu.host-associated.6.0\":\"MIGS.eu.host-associated.6.0\",\"MIGS.eu.human-associated.6.0\":\"MIGS.eu.human-associated.6.0\",\"MIGS.eu.human-gut.6.0\":\"MIGS.eu.human-gut.6.0\",\"MIGS.eu.human-oral.6.0\":\"MIGS.eu.human-oral.6.0\",\"MIGS.eu.human-skin.6.0\":\"MIGS.eu.human-skin.6.0\",\"MIGS.eu.human-vaginal.6.0\":\"MIGS.eu.human-vaginal.6.0\",\"MIGS.eu.hydrocarbon-cores.6.0\":\"MIGS.eu.hydrocarbon-cores.6.0\",\"MIGS.eu.hydrocarbon-fluids.swabs.6.0\":\"MIGS.eu.hydrocarbon-fluids.swabs.6.0\",\"MIGS.eu.microbial.6.0\":\"MIGS.eu.microbial.6.0\",\"MIGS.eu.miscellaneous.6.0\":\"MIGS.eu.miscellaneous.6.0\",\"MIGS.eu.plant-associated.6.0\":\"MIGS.eu.plant-associated.6.0\",\"MIGS.eu.sediment.6.0\":\"MIGS.eu.sediment.6.0\",\"MIGS.eu.soil.6.0\":\"MIGS.eu.soil.6.0\",\"MIGS.eu.symbiont-associated.6.0\":\"MIGS.eu.symbiont-associated.6.0\",\"MIGS.eu.wastewater.6.0\":\"MIGS.eu.wastewater.6.0\",\"MIGS.eu.water.6.0\":\"MIGS.eu.water.6.0\"},\n \"MIGS.vi\": {\"MIGS.vi.6.0\":\"MIGS.vi.6.0\",\"MIGS.vi.agriculture.6.0\":\"MIGS.vi.agriculture.6.0\",\"MIGS.vi.air.6.0\":\"MIGS.vi.air.6.0\",\"MIGS.vi.built.6.0\":\"MIGS.vi.built.6.0\",\"MIGS.vi.food-animal.6.0\":\"MIGS.vi.food-animal.6.0\",\"MIGS.vi.food-farm.env.6.0\":\"MIGS.vi.food-farm.env.6.0\",\"MIGS.vi.food-human.foods.6.0\":\"MIGS.vi.food-human.foods.6.0\",\"MIGS.vi.food-prod.facility.6.0\":\"MIGS.vi.food-prod.facility.6.0\",\"MIGS.vi.host-associated.6.0\":\"MIGS.vi.host-associated.6.0\",\"MIGS.vi.human-associated.6.0\":\"MIGS.vi.human-associated.6.0\",\"MIGS.vi.human-gut.6.0\":\"MIGS.vi.human-gut.6.0\",\"MIGS.vi.human-oral.6.0\":\"MIGS.vi.human-oral.6.0\",\"MIGS.vi.human-skin.6.0\":\"MIGS.vi.human-skin.6.0\",\"MIGS.vi.human-vaginal.6.0\":\"MIGS.vi.human-vaginal.6.0\",\"MIGS.vi.hydrocarbon-cores.6.0\":\"MIGS.vi.hydrocarbon-cores.6.0\",\"MIGS.vi.hydrocarbon-fluids.swabs.6.0\":\"MIGS.vi.hydrocarbon-fluids.swabs.6.0\",\"MIGS.vi.microbial.6.0\":\"MIGS.vi.microbial.6.0\",\"MIGS.vi.miscellaneous.6.0\":\"MIGS.vi.miscellaneous.6.0\",\"MIGS.vi.plant-associated.6.0\":\"MIGS.vi.plant-associated.6.0\",\"MIGS.vi.sediment.6.0\":\"MIGS.vi.sediment.6.0\",\"MIGS.vi.soil.6.0\":\"MIGS.vi.soil.6.0\",\"MIGS.vi.symbiont-associated.6.0\":\"MIGS.vi.symbiont-associated.6.0\",\"MIGS.vi.wastewater.6.0\":\"MIGS.vi.wastewater.6.0\",\"MIGS.vi.water.6.0\":\"MIGS.vi.water.6.0\"},\n \"MIMAG\": {\"MIMAG.6.0\":\"MIMAG.6.0\",\"MIMAG.agriculture.6.0\":\"MIMAG.agriculture.6.0\",\"MIMAG.air.6.0\":\"MIMAG.air.6.0\",\"MIMAG.built.6.0\":\"MIMAG.built.6.0\",\"MIMAG.food-animal.6.0\":\"MIMAG.food-animal.6.0\",\"MIMAG.food-farm.env.6.0\":\"MIMAG.food-farm.env.6.0\",\"MIMAG.food-human.foods.6.0\":\"MIMAG.food-human.foods.6.0\",\"MIMAG.food-prod.facility.6.0\":\"MIMAG.food-prod.facility.6.0\",\"MIMAG.host-associated.6.0\":\"MIMAG.host-associated.6.0\",\"MIMAG.human-associated.6.0\":\"MIMAG.human-associated.6.0\",\"MIMAG.human-gut.6.0\":\"MIMAG.human-gut.6.0\",\"MIMAG.human-oral.6.0\":\"MIMAG.human-oral.6.0\",\"MIMAG.human-skin.6.0\":\"MIMAG.human-skin.6.0\",\"MIMAG.human-vaginal.6.0\":\"MIMAG.human-vaginal.6.0\",\"MIMAG.hydrocarbon-cores.6.0\":\"MIMAG.hydrocarbon-cores.6.0\",\"MIMAG.hydrocarbon-fluids.swabs.6.0\":\"MIMAG.hydrocarbon-fluids.swabs.6.0\",\"MIMAG.microbial.6.0\":\"MIMAG.microbial.6.0\",\"MIMAG.miscellaneous.6.0\":\"MIMAG.miscellaneous.6.0\",\"MIMAG.plant-associated.6.0\":\"MIMAG.plant-associated.6.0\",\"MIMAG.sediment.6.0\":\"MIMAG.sediment.6.0\",\"MIMAG.soil.6.0\":\"MIMAG.soil.6.0\",\"MIMAG.symbiont-associated.6.0\":\"MIMAG.symbiont-associated.6.0\",\"MIMAG.wastewater.6.0\":\"MIMAG.wastewater.6.0\",\"MIMAG.water.6.0\":\"MIMAG.water.6.0\"},\n \"MIMARKS.specimen\": {\"MIMARKS.specimen.6.0\":\"MIMARKS.specimen.6.0\",\"MIMARKS.specimen.agriculture.6.0\":\"MIMARKS.specimen.agriculture.6.0\",\"MIMARKS.specimen.air.6.0\":\"MIMARKS.specimen.air.6.0\",\"MIMARKS.specimen.built.6.0\":\"MIMARKS.specimen.built.6.0\",\"MIMARKS.specimen.food-animal.6.0\":\"MIMARKS.specimen.food-animal.6.0\",\"MIMARKS.specimen.food-farm.env.6.0\":\"MIMARKS.specimen.food-farm.env.6.0\",\"MIMARKS.specimen.food-human.foods.6.0\":\"MIMARKS.specimen.food-human.foods.6.0\",\"MIMARKS.specimen.food-prod.facility.6.0\":\"MIMARKS.specimen.food-prod.facility.6.0\",\"MIMARKS.specimen.host-associated.6.0\":\"MIMARKS.specimen.host-associated.6.0\",\"MIMARKS.specimen.human-associated.6.0\":\"MIMARKS.specimen.human-associated.6.0\",\"MIMARKS.specimen.human-gut.6.0\":\"MIMARKS.specimen.human-gut.6.0\",\"MIMARKS.specimen.human-oral.6.0\":\"MIMARKS.specimen.human-oral.6.0\",\"MIMARKS.specimen.human-skin.6.0\":\"MIMARKS.specimen.human-skin.6.0\",\"MIMARKS.specimen.human-vaginal.6.0\":\"MIMARKS.specimen.human-vaginal.6.0\",\"MIMARKS.specimen.hydrocarbon-cores.6.0\":\"MIMARKS.specimen.hydrocarbon-cores.6.0\",\"MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0\":\"MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0\",\"MIMARKS.specimen.microbial.6.0\":\"MIMARKS.specimen.microbial.6.0\",\"MIMARKS.specimen.miscellaneous.6.0\":\"MIMARKS.specimen.miscellaneous.6.0\",\"MIMARKS.specimen.plant-associated.6.0\":\"MIMARKS.specimen.plant-associated.6.0\",\"MIMARKS.specimen.sediment.6.0\":\"MIMARKS.specimen.sediment.6.0\",\"MIMARKS.specimen.soil.6.0\":\"MIMARKS.specimen.soil.6.0\",\"MIMARKS.specimen.symbiont-associated.6.0\":\"MIMARKS.specimen.symbiont-associated.6.0\",\"MIMARKS.specimen.wastewater.6.0\":\"MIMARKS.specimen.wastewater.6.0\",\"MIMARKS.specimen.water.6.0\":\"MIMARKS.specimen.water.6.0\"},\n \"MIMARKS.survey\": {\"MIMARKS.survey.agriculture.6.0\":\"MIMARKS.survey.agriculture.6.0\",\"MIMARKS.survey.air.6.0\":\"MIMARKS.survey.air.6.0\",\"MIMARKS.survey.built.6.0\":\"MIMARKS.survey.built.6.0\",\"MIMARKS.survey.food-animal.6.0\":\"MIMARKS.survey.food-animal.6.0\",\"MIMARKS.survey.food-farm.env.6.0\":\"MIMARKS.survey.food-farm.env.6.0\",\"MIMARKS.survey.food-human.foods.6.0\":\"MIMARKS.survey.food-human.foods.6.0\",\"MIMARKS.survey.food-prod.facility.6.0\":\"MIMARKS.survey.food-prod.facility.6.0\",\"MIMARKS.survey.host-associated.6.0\":\"MIMARKS.survey.host-associated.6.0\",\"MIMARKS.survey.human-associated.6.0\":\"MIMARKS.survey.human-associated.6.0\",\"MIMARKS.survey.human-gut.6.0\":\"MIMARKS.survey.human-gut.6.0\",\"MIMARKS.survey.human-oral.6.0\":\"MIMARKS.survey.human-oral.6.0\",\"MIMARKS.survey.human-skin.6.0\":\"MIMARKS.survey.human-skin.6.0\",\"MIMARKS.survey.human-vaginal.6.0\":\"MIMARKS.survey.human-vaginal.6.0\",\"MIMARKS.survey.hydrocarbon-cores.6.0\":\"MIMARKS.survey.hydrocarbon-cores.6.0\",\"MIMARKS.survey.hydrocarbon-fluids.swabs.6.0\":\"MIMARKS.survey.hydrocarbon-fluids.swabs.6.0\",\"MIMARKS.survey.microbial.6.0\":\"MIMARKS.survey.microbial.6.0\",\"MIMARKS.survey.miscellaneous.6.0\":\"MIMARKS.survey.miscellaneous.6.0\",\"MIMARKS.survey.plant-associated.6.0\":\"MIMARKS.survey.plant-associated.6.0\",\"MIMARKS.survey.sediment.6.0\":\"MIMARKS.survey.sediment.6.0\",\"MIMARKS.survey.soil.6.0\":\"MIMARKS.survey.soil.6.0\",\"MIMARKS.survey.symbiont-associated.6.0\":\"MIMARKS.survey.symbiont-associated.6.0\",\"MIMARKS.survey.wastewater.6.0\":\"MIMARKS.survey.wastewater.6.0\",\"MIMARKS.survey.water.6.0\":\"MIMARKS.survey.water.6.0\"},\n \"MIMS.me\": {\"MIMS.me.agriculture.6.0\":\"MIMS.me.agriculture.6.0\",\"MIMS.me.air.6.0\":\"MIMS.me.air.6.0\",\"MIMS.me.built.6.0\":\"MIMS.me.built.6.0\",\"MIMS.me.food-animal.6.0\":\"MIMS.me.food-animal.6.0\",\"MIMS.me.food-farm.env.6.0\":\"MIMS.me.food-farm.env.6.0\",\"MIMS.me.food-human.foods.6.0\":\"MIMS.me.food-human.foods.6.0\",\"MIMS.me.food-prod.facility.6.0\":\"MIMS.me.food-prod.facility.6.0\",\"MIMS.me.host-associated.6.0\":\"MIMS.me.host-associated.6.0\",\"MIMS.me.human-associated.6.0\":\"MIMS.me.human-associated.6.0\",\"MIMS.me.human-gut.6.0\":\"MIMS.me.human-gut.6.0\",\"MIMS.me.human-oral.6.0\":\"MIMS.me.human-oral.6.0\",\"MIMS.me.human-skin.6.0\":\"MIMS.me.human-skin.6.0\",\"MIMS.me.human-vaginal.6.0\":\"MIMS.me.human-vaginal.6.0\",\"MIMS.me.hydrocarbon-cores.6.0\":\"MIMS.me.hydrocarbon-cores.6.0\",\"MIMS.me.hydrocarbon-fluids.swabs.6.0\":\"MIMS.me.hydrocarbon-fluids.swabs.6.0\",\"MIMS.me.microbial.6.0\":\"MIMS.me.microbial.6.0\",\"MIMS.me.miscellaneous.6.0\":\"MIMS.me.miscellaneous.6.0\",\"MIMS.me.plant-associated.6.0\":\"MIMS.me.plant-associated.6.0\",\"MIMS.me.sediment.6.0\":\"MIMS.me.sediment.6.0\",\"MIMS.me.soil.6.0\":\"MIMS.me.soil.6.0\",\"MIMS.me.symbiont-associated.6.0\":\"MIMS.me.symbiont-associated.6.0\",\"MIMS.me.wastewater.6.0\":\"MIMS.me.wastewater.6.0\",\"MIMS.me.water.6.0\":\"MIMS.me.water.6.0\"},\n \"MISAG\": {\"MISAG.6.0\":\"MISAG.6.0\",\"MISAG.agriculture.6.0\":\"MISAG.agriculture.6.0\",\"MISAG.air.6.0\":\"MISAG.air.6.0\",\"MISAG.built.6.0\":\"MISAG.built.6.0\",\"MISAG.food-animal.6.0\":\"MISAG.food-animal.6.0\",\"MISAG.food-farm.env.6.0\":\"MISAG.food-farm.env.6.0\",\"MISAG.food-human.foods.6.0\":\"MISAG.food-human.foods.6.0\",\"MISAG.food-prod.facility.6.0\":\"MISAG.food-prod.facility.6.0\",\"MISAG.host-associated.6.0\":\"MISAG.host-associated.6.0\",\"MISAG.human-associated.6.0\":\"MISAG.human-associated.6.0\",\"MISAG.human-gut.6.0\":\"MISAG.human-gut.6.0\",\"MISAG.human-oral.6.0\":\"MISAG.human-oral.6.0\",\"MISAG.human-skin.6.0\":\"MISAG.human-skin.6.0\",\"MISAG.human-vaginal.6.0\":\"MISAG.human-vaginal.6.0\",\"MISAG.hydrocarbon-cores.6.0\":\"MISAG.hydrocarbon-cores.6.0\",\"MISAG.hydrocarbon-fluids.swabs.6.0\":\"MISAG.hydrocarbon-fluids.swabs.6.0\",\"MISAG.microbial.6.0\":\"MISAG.microbial.6.0\",\"MISAG.miscellaneous.6.0\":\"MISAG.miscellaneous.6.0\",\"MISAG.plant-associated.6.0\":\"MISAG.plant-associated.6.0\",\"MISAG.sediment.6.0\":\"MISAG.sediment.6.0\",\"MISAG.soil.6.0\":\"MISAG.soil.6.0\",\"MISAG.symbiont-associated.6.0\":\"MISAG.symbiont-associated.6.0\",\"MISAG.wastewater.6.0\":\"MISAG.wastewater.6.0\",\"MISAG.water.6.0\":\"MISAG.water.6.0\"},\n \"MIUVIG\": {\"MIUVIG.6.0\":\"MIUVIG.6.0\",\"MIUVIG.agriculture.6.0\":\"MIUVIG.agriculture.6.0\",\"MIUVIG.air.6.0\":\"MIUVIG.air.6.0\",\"MIUVIG.built.6.0\":\"MIUVIG.built.6.0\",\"MIUVIG.food-animal.6.0\":\"MIUVIG.food-animal.6.0\",\"MIUVIG.food-farm.env.6.0\":\"MIUVIG.food-farm.env.6.0\",\"MIUVIG.food-human.foods.6.0\":\"MIUVIG.food-human.foods.6.0\",\"MIUVIG.food-prod.facility.6.0\":\"MIUVIG.food-prod.facility.6.0\",\"MIUVIG.host-associated.6.0\":\"MIUVIG.host-associated.6.0\",\"MIUVIG.human-associated.6.0\":\"MIUVIG.human-associated.6.0\",\"MIUVIG.human-gut.6.0\":\"MIUVIG.human-gut.6.0\",\"MIUVIG.human-oral.6.0\":\"MIUVIG.human-oral.6.0\",\"MIUVIG.human-skin.6.0\":\"MIUVIG.human-skin.6.0\",\"MIUVIG.human-vaginal.6.0\":\"MIUVIG.human-vaginal.6.0\",\"MIUVIG.hydrocarbon-cores.6.0\":\"MIUVIG.hydrocarbon-cores.6.0\",\"MIUVIG.hydrocarbon-fluids.swabs.6.0\":\"MIUVIG.hydrocarbon-fluids.swabs.6.0\",\"MIUVIG.microbial.6.0\":\"MIUVIG.microbial.6.0\",\"MIUVIG.miscellaneous.6.0\":\"MIUVIG.miscellaneous.6.0\",\"MIUVIG.plant-associated.6.0\":\"MIUVIG.plant-associated.6.0\",\"MIUVIG.sediment.6.0\":\"MIUVIG.sediment.6.0\",\"MIUVIG.soil.6.0\":\"MIUVIG.soil.6.0\",\"MIUVIG.symbiont-associated.6.0\":\"MIUVIG.symbiont-associated.6.0\",\"MIUVIG.wastewater.6.0\":\"MIUVIG.wastewater.6.0\",\"MIUVIG.water.6.0\":\"MIUVIG.water.6.0\"}\n },\n selected=\"Pathogen.cl.1.0\",\n ),\n ),\n # If GenBank checkbox checked then load Genbank Schema Options\n ui.panel_conditional(\n \"input.GenBank_checkbox\",\n ui.input_select(\n \"GenBank_schemas\",\n label=\"Select SeqSender GenBank Schema:\",\n choices=[\"COV\", \"FLU\", \"OTHER\"],\n selected=\"OTHER\",\n ),\n ),\n # If GISAID checkbox checked then load GISAID Database Options\n ui.panel_conditional(\n \"input.GISAID_checkbox\",\n ui.input_select(\n \"GISAID_databases\",\n label=\"Select GISAID Database:\",\n choices=[\"FLU\", \"COV\", \"POX\", \"ARBO\"],\n ),\n ),\n ),\n ),\n # Based on choices display config and metadata template\n ui.panel_conditional(\n \"input.BioSample_checkbox || input.SRA_checkbox || input.GenBank_checkbox || input.GISAID_checkbox\",\n ui.card(\n ui.card_header(\n ui.h4(\"Create Config File:\", style=\"display:inline-block;\"),\n shiny_tools.create_help_tooltip(\n id = \"config_tooltip\",\n description = \"Based on previous selections, required fields for the config file can change. The config file is used to generate the necessary files for submission.\",\n position=\"right\",\n ),\n ),\n ui.card(\n ui.page_fluid(\n div(ui.HTML(\"Submission:\"), style=\"margin-top:-20px\"),\n ui.panel_conditional(\n \"input.BioSample_checkbox || input.SRA_checkbox || input.GenBank_checkbox\",\n config_indent(1, \"NCBI:\", custom_style = \"\"),\n # Username\n config_indent(2, \"Username:\"),\n config_text_input(\"ncbi_config_username\", placeholder = \"NCBI FTP Username\", help_msg = ui.p(\"Username for your NCBI FTP account.\", ui.strong(\"Not your NCBI account username.\"))),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Password\n config_indent(2, \"Password:\"),\n config_text_input(\"ncbi_config_password\", placeholder = \"NCBI FTP Password\", help_msg = ui.p(\"Password for your NCBI FTP account.\", ui.strong(\"Not your NCBI account password.\"))),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Spuid Namespace\n config_indent(2, \"Spuid_Namespace:\"),\n config_text_input(\"ncbi_config_spuid_namespace\", placeholder = \"NCBI SPUID Namespace\", help_msg = ui.p(\"NCBI SPUID namespace. This should have been provided when you set up your submisison group for NCBI FTP submissions.\")),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # BioSample Package\n ui.panel_conditional(\n \"input.BioSample_checkbox\",\n config_indent(2, \"BioSample_Package:\"),\n div(\n ui.output_text(\"BioSample_Package_Name\",inline=True,),\n shiny_tools.create_help_tooltip(\"config_bs_package\", description = (\"This is predetermined based on database options selected.\"), position = \"none\"),\n style=\"display:inline-block;height:5px;\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n ),\n ui.panel_conditional(\n \"input.GenBank_checkbox\",\n # Automatically remove GenBank samples if failed to upload\n config_indent(2, \"GenBank_Auto_Remove_Failed_Samples:\"),\n div(ui.input_radio_buttons(\n \"ncbi_config_auto_remove_genbank\",\n label=None,\n choices=[True, False],\n inline=True,\n ),\n style=\"display:inline-block;height:5px;font-size:medium;\",\n ),\n shiny_tools.create_help_tooltip(\"ncbi_auto_remove\", description = (\"If you're submitting to GenBank via FTP, this will automatically remove sequences that fail to pass GenBank's validation and annotation, only if there are samples that did pass. A report file (\", ui.code(\"seq-edit-report.html\"), \") will also be generated explaining the reason why the samples were rejected.\"), position = \"none\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n ),\n # Publication title\n config_indent(2, \"Publication_Title:\"),\n config_text_input(\"ncbi_config_publication_title\", placeholder = \"Publication Title\", help_msg = ui.p(\"Publication title for your NCBI submissions. If your samples are part of a paper/article then that title should be used here, otherwise, use a descriptive title for the submissions you plan to make with this config file.\")),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Publication status\n config_indent(2, \"Publication_Status:\"),\n div(ui.input_radio_buttons(\n \"ncbi_config_publication_status\",\n label=None,\n choices=[\"Unpublished\", \"In-press\", \"Published\"],\n inline=True,\n ),\n style=\"display:inline-block;height:5px;font-size:medium;\",\n ),\n shiny_tools.create_help_tooltip(\"ncbi_sub_status\", description = (\"If a paper is included with your submission then use \\\"In-press\\\" or \\\"Published\\\". If you haven't or aren't publishing a paper with your samples then use \\\"Unpublished\\\".\"), position = \"none\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n ui.panel_conditional(\n \"input.GenBank_checkbox && input.GISAID_checkbox\",\n # NCBI submission position\n config_indent(2, \"Submission_Position:\"),\n div(ui.input_radio_buttons(\n \"ncbi_submission_position\",\n label=None,\n choices={1:\"1\", 2:\"2\", \"\":\"None\"},\n selected=1,\n inline=True,\n ),\n style=\"display:inline-block;height:5px;font-size:medium;\",\n ),\n shiny_tools.create_help_tooltip(\"ncbi_sub_pos\", description = (\"If submitting to both GISAID and NCBI, then determine the order you want the accessions linked between databases. If you do not want to link accessions and want GenBank and GISAID submissions to be made at the same time then select \", ui.strong(\"None\"), \".\"), position = \"none\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n ),\n # Specified release date\n config_indent(2, \"Specified_Release_Date:\"),\n config_text_input(\"ncbi_config_release_date\", placeholder = \"Release Date\", help_msg = \"Release date for NCBI submissions. Allows you to specify when should NCBI make your submissions public, allowing you to submit your samples ahead of a planned release. If you want to release samples immediately leave blank or specify a date as \\\"YYYY-MM-DD\\\" to release at a specific date. This field also takes the option of a numeric value followed by one of the key words \\\"days\\\", \\\"weeks\\\", or \\\"months\\\", to have SeqSender create a specified release date based on when SeqSender is ran.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Link sample between NCBI databases\n config_indent(2, \"Link_Sample_Between_NCBI_Databases:\"),\n div(ui.input_radio_buttons(\n \"ncbi_config_link_samples\",\n label=None,\n choices=[True, False],\n inline=True,\n ),\n style=\"display:inline-block;height:5px;font-size:medium;\",\n ),\n shiny_tools.create_help_tooltip(\"ncbi_link_sample\", description = (\"If you're submitting your samples to multiple NCBI databases, do you want your sample's data linked between each database?\"), position = \"none\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Description category\n config_indent(2, \"Description:\", custom_style = \"\"),\n # Organization category\n config_indent(3, \"Organization\", custom_style = \"\"),\n # Organization role\n config_indent(4, \"Role:\"),\n config_text_input(\"ncbi_config_role\", placeholder=\"Organization Role\", help_msg = \"Provided by NCBI when setting up your FTP account.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Organization type\n config_indent(4, \"Type:\"),\n config_text_input(\"ncbi_config_type\", placeholder= \"Organization Type\", help_msg = \"Provided by NCBI when setting up your FTP account.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Organization name\n config_indent(4, \"Name:\"),\n config_text_input(\"ncbi_config_org_name\", placeholder = \"Organization Name\", help_msg = \"Submitting group's name.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # Address category\n config_indent(5, \"Address:\", custom_style = \"\"),\n # submitter affiliation\n config_indent(6, \"Affil:\"),\n config_text_input(\"ncbi_config_affil\", placeholder = \"Submitter Affiliation\", help_msg = \"Submitting organization's full name.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter division\n config_indent(6, \"Div:\"),\n config_text_input(\"ncbi_config_div\", placeholder = \"Submitter Division\", help_msg = \"Submitting department/laboratory name.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter street\n config_indent(6, \"Street:\"),\n config_text_input(\"ncbi_config_street\", placeholder = \"Submitting Organization Street Name\", help_msg = \"Street name.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter city\n config_indent(6, \"City:\"),\n config_text_input(\"ncbi_config_city\", placeholder = \"Submitting Organization City\", help_msg = \"City.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter state\n config_indent(6, \"Sub:\"),\n config_text_input(\"ncbi_config_state\", placeholder = \"Submitting Organization State/Providence\", help_msg = \"State/Providence.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter zipcode\n config_indent(6, \"Postal_Code:\"),\n config_text_input(\"ncbi_config_postal\", placeholder = \"Submitting Organization Postal Code\", help_msg = \"Postal code.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter country\n config_indent(6, \"Country:\"),\n config_text_input(\"ncbi_config_country\", placeholder = \"Submitting Organization Country\", help_msg = \"Country\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter contact email\n config_indent(6, \"Email:\"),\n config_text_input(\"ncbi_config_email\", placeholder = \"Submitting Organization Email\", help_msg = \"Email for someone to contact you at if they have questions about your samples.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter contact phone\n config_indent(6, \"Phone:\"),\n config_text_input(\"ncbi_config_phone\", placeholder = \"Submitting Organization Phone\", help_msg = \"Phone number for someone to contact you at if they have questions about your samples.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter point of contact\n config_indent(5, \"Submitter\", custom_style = \"\"),\n # submitter poc email\n config_indent(6, \"Email:\"),\n config_text_input(\"ncbi_sub_email_one\", \"Submitter Contact Email\", help_msg = \"Email for someone at NCBI to contact about issues during the submission process.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # submitter poc email\n config_indent(6, \"Alt_Email:\"),\n config_text_input(\"ncbi_sub_email_two\", \"Submitter Contact Email\", help_msg = \"Alternate email for someone at NCBI to contact about issues during the submission process.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n # poc name\n config_indent(6, \"Name:\", custom_style = \"\"),\n # poc name first\n config_indent(7, \"First:\"),\n config_text_input(\"ncbi_config_first_name\", placeholder = \"Submitter First Name\", help_msg = \"Who is making the submission: First Name.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n config_indent(7, \"Last:\"),\n config_text_input(\"ncbi_config_last_name\", placeholder = \"Submitter Last Name\", help_msg = \"Who is making the submission: Last Name.\"),\n ),\n ui.panel_conditional(\n \"input.GISAID_checkbox\",\n config_indent(1, \"GISAID\", custom_style = \"\"),\n config_indent(2, \"Client-Id:\"),\n config_text_input(\"gisaid_config_client\", placeholder = \"GISAID Client ID\", help_msg = \"Provided by GISAID when authorized to make FTP submissions.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n config_indent(2, \"Username:\"),\n config_text_input(\"gisaid_config_username\", placeholder = \"GISAID Username\", help_msg = \"Username to login to GISAID.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n config_indent(2, \"Password:\"),\n config_text_input(\"gisaid_config_password\", placeholder = \"GISAID Password\", help_msg = \"Password to login to GISAID.\"),\n div(ui.HTML(\"
\"), style=\"margin-top:-25px;\"),\n ui.panel_conditional(\n \"input.GISAID_checkbox && input.GenBank_checkbox\",\n config_indent(2, \"Submission_Position:\"),\n div(ui.input_radio_buttons(\n \"gisaid_submission_position\",\n label=None,\n choices={1:\"1\", 2:\"2\", \"\":\"None\"},\n inline=True,\n selected=2,\n ),\n style=\"display:inline-block;height:5px;font-size:medium;\",\n ),\n shiny_tools.create_help_tooltip(\"gisaid_sub_pos\", description = (\"If submitting to both GISAID and NCBI, then determine the order you want the accessions linked between databases. If you do not want to link accessions and want GenBank and GISAID submissions to be made at the same time then select \", ui.strong(\"None\"), \".\"), position = \"none\"),\n ),\n ),\n ),\n style=yaml_css,\n ),\n ui.download_button(\"download_config\", \"Download Config File\"),\n ),\n ui.card(\n ui.card_header(\n ui.h4(\"Metadata Template:\", style=\"display:inline-block;\"),\n ui.tooltip(\n ui.p(\"\u2753\"),\n \"Additional info\",\n id=\"metadata_tooltip\",\n style=\"display:inline-block;float:right;\",\n ),\n ),\n ui.HTML(\n \"Note: More columns may be available not displayed in this list. To add a column not here during submission, add it matching the style of the other columns related to the database: database_prefix-column_name.\"\n ),\n ui.output_table(\"load_database_metadata_dataframe\"),\n ui.HTML(\n \"\"\"\nMetadata Sections Legend:\n
SeqSender: No prefix | BioSample: bs- | SRA: sra- | GenBank: gb- | GISAID: gs-
\n\"\"\"\n ),\n ui.download_button(\"download_metadata\", \"Download Metadata Template\"),\n ),\n ),\n]\n", "type": "text"}, {"name": "shiny_tools.py", "content": "from shiny import App, Inputs, Outputs, Session, render, req, ui, reactive\nfrom htmltools import TagList, div\n\n\nterminal_css = \"\"\n\ndef software_requirements(version):\n requirements = \"\"\"
\nSOFTWARE REQUIREMENTS:
\n\n- Linux (64-bit) or Mac OS X (64-bit)
\n- Git version 2.25.1 or later
\n- Standard utilities: curl, tar, unzip
\n\"\"\"\n if version == \"Local\":\n return ui.HTML(requirements + \"\"\"
\"\"\")\n elif version == \"Docker\":\n return ui.HTML(\n requirements\n + \"\"\"\nDocker version 20.10.14 or later\n[Optional] Docker Compose version 2.21 or later\n\n\"\"\"\n )\n elif version == \"Singularity\":\n return ui.HTML(\n requirements\n + \"\"\"\nSingularity version 3.8.7 or later\n\n\"\"\"\n )\n else:\n return \"\"\n\ndef seqsender_help_output_msg(version):\n message = \"\"\"\nusage: seqsender.py [-h] {prep,submit,check_submission_status,template,update_biosample,version} ...\nAutomate the process of batch uploading consensus sequences and metadata to databases of your choices
\npositional arguments:
\n | {prep,submit,check_submission_status,template,update_biosample,version}
\noptional arguments:
\n | -h, --help | show this help message and exit
\n\"\"\"\n if version == \"Local\":\n command = (\n \"\"\"(seqsender) [USER@server seqsender]$ python seqsender.py --help
\"\"\"\n )\n elif version == \"Docker\":\n command = \"\"\"
docker exec -it seqsender bash seqsender-kickoff --help
\"\"\"\n elif version == \"Singularity\":\n command = \"\"\"
singularity exec ~/singularity/seqsender.sif seqsender-kickoff --help
\"\"\"\n else:\n command = \"\"\"
\"\"\"\n return ui.card(ui.HTML(command + message), style=terminal_css)\n\n\ndatabases_parameter = [\n ui.strong(\"Databases\"),\n ui.tags.ul(\n ui.tags.li(\n ui.strong(\n ui.code(\"--biosample\"),\n \" | \",\n ui.code(\"-b\"),\n ),\n ui.tags.ul(\n \" Generate files required for submission to \",\n ui.strong(\"NCBI Database: BioSample\"),\n \".\",\n inline=True,\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"--sra\"),\n \" | \",\n ui.code(\"-s\"),\n ),\n ui.tags.ul(\n \" Generate files required for submission to \",\n ui.strong(\"NCBI Database: SRA\"),\n \".\",\n inline=True,\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"--genbank\"),\n \" | \",\n ui.code(\"-n\"),\n ),\n ui.tags.ul(\n \" Generate files required for submission to \",\n ui.strong(\"NCBI Database: GenBank\"),\n \".\",\n inline=True,\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"--gisaid\"),\n \" | \",\n ui.code(\"-g\"),\n ),\n ui.tags.ul(\n \" Generate files required for submission to \",\n ui.strong(\"GISAID\"),\n \". Not available as an option when using the organism parameter \",\n ui.code(\"OTHER\"),\n \".\",\n inline=True,\n ),\n ),\n ),\n]\n\norganisms_parameter = [\n ui.strong(\"Organism\"),\n ui.div(\n ui.strong(ui.code(\"--organism \"), \"{'FLU', 'COV', 'POX', 'ARBO', 'OTHER'}\"),\n inline=True,\n ),\n ui.p(\n \"Organism selection adjusts seqsender submission process for organism specific criteria.\"\n ),\n ui.tags.ul(\n ui.tags.li(\n ui.strong(\n ui.code(\"FLU\"),\n ),\n ui.tags.ul(\n \" For \",\n ui.strong(\"Influenza A virus\"),\n \", enables FTP GenBank submission and GISAID CLI submission.\",\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"COV\"),\n ),\n ui.tags.ul(\n \" For \",\n ui.strong(\"SARS-CoV-2\"),\n \", enables FTP GenBank submission and GISAID CLI submission.\",\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"POX\"),\n ),\n ui.tags.ul(\n \" For \",\n ui.strong(\"Mpox\"),\n \" (monkeypox), enables GISAID CLI submission.\",\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"ARBO\"),\n ),\n ui.tags.ul(\n \" For \", ui.strong(\"Arbovirus\"), \", enables GISAID CLI submission.\"\n ),\n ),\n ui.tags.li(\n ui.strong(\n ui.code(\"OTHER\"),\n ),\n ui.tags.ul(\n \" For any organism that does not have special submission options.\"\n ),\n ),\n ),\n]\n\nsubmission_directory_parameter = ui.div(\n ui.strong(ui.code(\"--submission_dir\")),\n ui.tags.ul(\"Directory where all files required for submission are stored.\"),\n)\n\nsubmission_name_parameter = ui.div(\n ui.strong(ui.code(\"--submission_name\")),\n ui.tags.ul(\"Unique name to use for submission. Used as a unique identifier for submission to databases and to name the files created for submission.\"),\n)\n\nconfig_parameter = ui.div(\n ui.strong(ui.code(\"--config_file\")),\n ui.tags.ul(\"Full path to config file if not stored in \", ui.code(\"--submission_dir\"), \" location.\"),\n)\n\nmetadata_parameter = ui.div(\n ui.strong(ui.code(\"--metadata_file\")),\n ui.tags.ul(\"Full path to metadata file if not stored in \", ui.code(\"--submission_dir\"), \" location.\"),\n)\n\nfasta_parameter = ui.div(\n ui.strong(ui.code(\"--fasta_file\")),\n ui.tags.ul(\"Full path to fasta file if not stored in \", ui.code(\"--submission_dir\"), \" location.\"),\n)\n\ntable2asn_parameter = ui.div(\n ui.strong(ui.code(\"--table2asn\")),\n ui.tags.ul(\"Flag to enable a \\\"table2asn\\\" submission for GenBank. Automatically enabled for organisms other than \\\"FLU\\\" and \\\"COV\\\".\"),\n)\n\ngff_parameter = ui.div(\n ui.strong(ui.code(\"--gff_file\")),\n ui.tags.ul(\"Full path to gff file if not stored in \", ui.code(\"--submission_dir\"), \" location.\"),\n)\n\ntest_parameter = ui.div(\n ui.strong(ui.code(\"--test\")),\n ui.tags.ul(\"Flag to enable a \\\"test\\\" submission to each database selected.\"),\n)\n\nvalidation_parameter = ui.div(\n ui.strong(ui.code(\"--skip_validation\")),\n ui.tags.ul(\"Flag to skip pandera validation for \", ui.code(\"--metadata_file\"), \". Warning, skipping validation can cause unexpected errors when missing required data.\"),\n)\n\ndef seqsender_submit_help_output_msg(version):\n message = \"\"\"\nusage: seqsender.py submit [-h] [--biosample] [--sra] [--genbank] [--gisaid] --organism {FLU,COV,POX,ARBO,OTHER}\n--submission_name SUBMISSION_NAME --submission_dir SUBMISSION_DIR --config_file CONFIG_FILE --metadata_file METADATA_FILE\n--fasta_file FASTA_FILE [--table2asn] [--gff_file GFF_FILE] [--test]
\nCreate submission files and then batch uploading them to databases of choices.
\noptional arguments:
\n -h, --help | | | \nshow this help message and exit
\n --biosample, -b | | \nSubmit to BioSample database. (default: )
\n --sra, -s | | | \nSubmit to SRA database. (default: )
\n --genbank, -n | | \nSubmit to Genbank database. (default: )
\n --gisaid, -g | | | \nSubmit to GISAID database. (default: )
\n --organism {FLU,COV,POX,ARBO,OTHER}
| | | | \nType of organism data (default: FLU)
\n --submission_name SUBMISSION_NAME
| | | | \nName of the submission (default: None)
\n --submission_dir SUBMISSION_DIR
| | | | \nDirectory to where all required files (such as metadata, fasta, etc.) are stored (default: None)
\n --config_file CONFIG_FILE
| | | | \nConfig file stored in submission directory (default: None)
\n --metadata_file METADATA_FILE
| | | | \nMetadata file stored in submission directory (default: None)
\n --fasta_file FASTA_FILE
| | | | \nFasta file stored in submission directory (default: None)
\n --table2asn | | \nWhether to prepare a Table2asn submission. (default: False)
\n --gff_file GFF_FILE \nAn annotation file to add to a Table2asn submission (default: None)
\n --test | | | | \nWhether to perform a test submission. (default: False)
\n\"\"\"\n if version == \"Local\":\n command = \"\"\"
(seqsender) [USER@server seqsender]$ python seqsender.py submit --help
\"\"\"\n elif version == \"Docker\":\n command = (\n \"\"\"
docker exec -it seqsender bash seqsender-kickoff submit --help
\"\"\"\n )\n elif version == \"Singularity\":\n command = \"\"\"
singularity exec ~/singularity/seqsender.sif seqsender-kickoff submit --help
\"\"\"\n else:\n command = \"\"\"\"\"\"\n return ui.card(ui.HTML(command + message), style=terminal_css)\n\n# Returns parameters in format for code example and descriptive list of parameter\ndef get_parameters(command, output):\n command_string = \"\"\n parameters = []\n # Databases parameter\n if command in [\"prep\", \"submit\", \"test_data\"]:\n command_string += \" [<databases>]\"\n parameters.append(databases_parameter)\n # Organisms parameter\n if command in [\"prep\", \"submit\", \"test_data\"]:\n command_string += \" [--organism <organism>]\"\n parameters.append(organisms_parameter)\n # Submission_dir parameter\n if command in [\"prep\", \"submit\", \"test_data\", \"submission_status\"]:\n command_string += \" [--submission_dir <directory_path>]\"\n parameters.append(submission_directory_parameter)\n # Add submission_name parameter\n if command in [\"prep\", \"submit\", \"submission_status\"]:\n command_string += \" [--submission_name <name>]\"\n parameters.append(submission_name_parameter)\n # Add config_file parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--config_file <config_file_path>]\"\n parameters.append(config_parameter)\n # Add metadata_file parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--metadata_file <metadata_file_path>]\"\n parameters.append(metadata_parameter)\n # Add fasta_file parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--fasta_file <fasta_file_path>]\"\n parameters.append(fasta_parameter)\n # Add table2asn parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--table2asn]\"\n parameters.append(table2asn_parameter)\n # Add gff_file parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--gff_file <gff_file_path>]\"\n parameters.append(gff_parameter)\n # Add test parameter\n if command in [\"submit\"]:\n command_string += \" [--test]\"\n parameters.append(test_parameter)\n # Add skip pandera validation parameter\n if command in [\"prep\", \"submit\"]:\n command_string += \" [--skip_validation]\"\n parameters.append(validation_parameter)\n if output == \"string\":\n return command_string\n elif output == \"description\":\n return parameters\n\ndef seqsender_example_command(platform, command, flags):\n # Platform specific layout\n message = \"
\"\n if platform == \"local\":\n message += \"python seqsender.py \"\n elif platform == \"docker\":\n message += \"docker exec -it seqsender bash seqsender-kickoff \"\n elif platform == \"singularity\":\n message += \"singularity exec ~/singularity/seqsender.sif seqsender-kickoff \"\n else:\n message += \"\"\n # Seqsender command\n message += command\n ### Add parameters\n message += get_parameters(command, \"string\") + \"
\"\n return ui.HTML(message)\n\ndef command_submenu_accordion(command):\n submenu = []\n open_tabs = []\n parameters = get_parameters(command, \"description\")\n if len(parameters) > 0:\n submenu.append(ui.accordion_panel(\"Parameters\", parameters))\n open_tabs.append(\"Parameters\")\n notes = [] # notes function\n if len(notes) > 0:\n submenu.append(ui.accordion_panel(\"Notes\", notes))\n open_tabs.append(\"Notes\")\n examples = [] # examples function\n if len(examples) > 0:\n submenu.append(ui.accordion_panel(\"Examples\", examples))\n if len(submenu) > 0:\n return ui.accordion(*submenu, open = open_tabs)\n else:\n return \"\"\n\ndef command_accordion_panel(command, description):\n return ui.accordion_panel(\n command.capitalize(),\n ui.p(\n \"The \",\n ui.code(command),\n description,\n ),\n seqsender_example_command(\"local\", command, \"\"),\n command_submenu_accordion(command),)\n\ndef file_output_column_info(column_name, description, controlled_fields):\n list_item = ui.tags.li(\n ui.strong(\n ui.code(column_name),\n ),\n ui.tags.ul(\n ui.p(description),\n file_output_controlled_field_info(controlled_fields),\n )\n )\n return list_item\n\ndef file_output_controlled_field_info(controlled_fields):\n keywords = []\n if controlled_fields:\n for key, description in controlled_fields:\n keywords.append(ui.tags.li(ui.p(ui.strong(key, \": \"), description), style=\"margin-top:10px;\"))\n return keywords\n\ndef create_help_tooltip(id, description, position):\n return ui.tooltip(ui.p(\"\u2753\"),description,id=id,style=\"display:inline-block;float:\" + position + \";\",)\n", "type": "text"}, {"name": "templates/config.biosample.Beta-lactamase.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-beta_lactamase_family,Required,Specify the beta-lactamase family for this gene.\nbs-carbapenemase,Required,\"Does the enzyme exhibit carbapenemase activity? If the enzyme does exhibit carbapenemase activity, the response should be \"\"yes\"\", otherwise \"\"no.\"\"\"\nbs-edta_inhibitor_tested,Required,\"Was carbapenemase activity tested in the presence of EDTA? If carbapenemase activity was tested in the presence of EDTA, the response should be \"\"yes\"\", otherwise \"\"no\u201d.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lab_host,Optional,\"Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained, e.g., Escherichia coli DH5a, or Homo sapiens HeLa cells\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Human.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-age,Required,\"age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees\"\nbs-biomaterial_provider,Required,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-sex,Required,physical sex of sampled organism\nbs-tissue,Required,Type of tissue the sample was taken from.\nbs-cell_line,Optional,Name of the cell line.\nbs-cell_subtype,Optional,\nbs-cell_type,Optional,Type of cell of the sample or from which the sample was obtained.\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-dev_stage,Optional,Developmental stage at the time of sampling.\nbs-disease,Optional,\"list of diseases diagnosed; can include multiple diagnoses. the value of the field depends on host; for humans the terms should be chosen from DO (Disease Ontology), free text for non-human. For DO terms, please see http://gemina.svn.sourceforge.net/viewvc/gemina/trunk/Gemina/ontologies/gemina_symptom.obo?view=log\"\nbs-disease_stage,Optional,stage of disease at the time of sampling.\nbs-ethnicity,Optional,ethnicity of the subject\nbs-health_state,Optional,Health or disease status of sample at time of collection\nbs-karyotype,Optional,\nbs-phenotype,Optional,\"Phenotype of sampled organism. For Phenotypic quality Ontology (PATO) (v1.269) terms, please see http://bioportal.bioontology.org/visualize/44601\"\nbs-population,Optional,\"for human: ; for plants: filial generation, number of progeny, genetic structure\"\nbs-race,Optional,\nbs-sample_type,Optional,\"Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly\"\nbs-treatment,Optional,\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Invertebrate.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-breed,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". breed name - chiefly used in domesticated animals or plants\"\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-tissue,Required,Type of tissue the sample was taken from.\nbs-age,Optional,\"age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-biomaterial_provider,Optional,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-dev_stage,Optional,Developmental stage at the time of sampling.\nbs-env_broad_scale,Optional,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-identified_by,Optional,name of the taxonomist who identified the specimen\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sex,Optional,physical sex of sampled organism\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-escalator,Optional,The number of escalators within the built structure\nbs-estimated_size,Optional,Estimated size of genome\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.ba.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,Required,microbial or eukaryotic strain name\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-num_replicons,Required,Number of replicons in nuclear genome\nbs-ref_biomaterial,Required,Primary publication or genome report\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-estimated_size,Optional,Estimated size of genome\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.eu.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-ploidy,Optional,\"Ploidy level of genome (eg., haploid, diploid)\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-propagation,Optional,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-escalator,Optional,The number of escalators within the built structure\nbs-estimated_size,Optional,Estimated size of genome\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIGS.vi.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-propagation,Required,phage: lytic/lysogenic/temperate/obligately lytic; plasmid: incompatibility group; eukaryote: asexual/sexual\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-encoded_traits,Optional,Traits like antibiotic resistance/xenobiotic degration phenotypes/converting phage genes\nbs-estimated_size,Optional,Estimated size of genome\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-num_replicons,Optional,Number of replicons in nuclear genome\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_taxid,Required,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-pathogenicity,Required,To what is the entity pathogenic\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isol_growth_condt,Optional,PMID or url for isolation and growth condition specifications\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-douche,Optional,date of most recent douche\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMAG.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-derived_from,Optional,Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) (SAMNxxxxxxxx).\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-metagenome_source,Optional,\"describes the original source of a metagenome assembled genome (MAG). Examples: soil metagenome, gut metagenome\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_taxid,Required,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-pathogenicity,Required,To what is the entity pathogenic\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-ethnicity,Optional,ethnicity of the subject\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.specimen.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isol_growth_condt,Required,PMID or url for isolation and growth condition specifications\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-extrachrom_elements,Optional,Plasmids that have significance phenotypic consequence\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_taxid,Required,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-pathogenicity,Required,To what is the entity pathogenic\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isol_growth_condt,Optional,PMID or url for isolation and growth condition specifications\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-ethnicity,Optional,ethnicity of the subject\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMARKS.survey.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_taxid,Required,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-pathogenicity,Required,To what is the entity pathogenic\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isol_growth_condt,Optional,PMID or url for isolation and growth condition specifications\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-ethnicity,Optional,ethnicity of the subject\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-ethnicity,Optional,ethnicity of the subject\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-ethnicity,Optional,ethnicity of the subject\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIMS.me.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_taxid,Required,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-pathogenicity,Required,To what is the entity pathogenic\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isol_growth_condt,Optional,PMID or url for isolation and growth condition specifications\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MISAG.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-wga_amp_appr,Optional,\"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.agriculture.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_source,Required,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_age,Required,Age of host at the time of sampling\nbs-host_common_name,Required,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_genotype,Required,\nbs-host_height,Required,the height of subject\nbs-host_length,Required,the length of subject\nbs-host_life_stage,Required,description of host life stage\nbs-host_phenotype,Required,\nbs-host_tot_mass,Required,\"total mass of the host at collection, the unit depends on host\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-microbial_biomass,Required,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Required,reference or method used in determining microbial biomass\nbs-samp_store_dur,Required,\nbs-samp_store_loc,Required,\nbs-sieving,Required,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-soil_type,Required,soil series name or other lower-level classification\nbs-soil_type_meth,Required,reference or method used in determining soil series name or other lower-level classification\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-store_cond,Required,explain how and for how long the soil sample was stored before DNA extraction.\nbs-temp,Required,temperature of the sample at time of sampling\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-isol_growth_condt,Optional,PMID or url for isolation and growth condition specifications\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-photosynt_activ,Optional,Measurement of photosythetic activity (i.e. leaf gas exchange / chlorophyll fluorescence emissions / reflectance / transpiration) Please also include the term method term detailing the method of activity measurement\nbs-photosynt_activ_meth,Optional,Reference or method used in measurement of photosythetic activity\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_depth,Optional,\"The vertical distance below local surface, e.g., for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples\"\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-trophic_level,Optional,\"Feeding position in food chain (eg., chemolithotroph)\"\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.air.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-barometric_press,Optional,force per unit area exerted against a surface by the weight of air above that surface\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-carb_dioxide,Optional,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-carb_monoxide,Optional,carbon monoxide (gas) amount or concentration at the time of sampling\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-oxygen,Optional,oxygen (gas) amount or concentration at the time of sampling\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pollutants,Optional,\"pollutant types and, amount or concentrations measured at the time of sampling; can report multiple pollutants by entering numeric values preceded by name of pollutant\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.built.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture\nbs-air_temp,Required,temperature of the air at the time of sampling\nbs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used\nbs-building_setting,Required,location (geography) where a building is set\nbs-carb_dioxide,Required,carbon dioxide (gas) amount or concentration at the time of sampling\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-filter_type,Required,device which removes solid particulates or airborne molecular contaminants\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-heat_cool_type,Required,methods of conditioning or heating a room or building\nbs-indoor_space,Required,\"a distinguishable space within a structure, the purpose for which discrete areas of a building is used\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-light_type,Required,\"application of light to achieve some practical or aesthetic effect. Lighting includes the use of both artificial light sources such as lamps and light fixtures, as well as natural illumination by capturing daylight. Can also include absence of light\"\nbs-occup_samp,Required,number of occupants present at time of sample within the given space\nbs-occupant_dens_samp,Required,average number of occupants at time of sampling per square footage\nbs-organism_count,Required,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-rel_air_humidity,Required,\"partial vapor and air pressure, density of the vapor and air, or by the actual mass of the vapor and air\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-space_typ_state,Required,customary or normal state of the space\nbs-typ_occupant_dens,Required,customary or normal density of occupants\nbs-ventilation_type,Required,ventilation system used in the sampled premises\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-address,Optional,The street name and building number where the sampling occurred\nbs-adj_room,Optional,\"List of rooms (room number, room name) immediately adjacent to the sampling room\"\nbs-aero_struc,Optional,\"Aerospace structures typically consist of thin plates with stiffeners for the external surfaces, bulkheads and frames to support the shape and fasteners such as welds, rivets, screws and bolts to hold the components together, e.g., plane, glider\"\nbs-amount_light,Optional,\"The unit of illuminance and luminous emittance, measuring luminous flux per unit area\"\nbs-arch_struc,Optional,\"An architectural structure is a human-made, free-standing, immobile outdoor construction, e.g., building, shed, home\"\nbs-avg_dew_point,Optional,The average of dew point measures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-avg_temp,Optional,The average of temperatures taken at the beginning of every hour over a 24 hour period on the sampling day\nbs-bathroom_count,Optional,The number of bathrooms in the building\nbs-bedroom_count,Optional,The number of bedrooms in the building\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-build_docs,Optional,\"The building design, construction and operation documents, e.g., building information model, commissioning report, complaint logs, contract administration, cost estimate, janitorial schedules or logs, maintenance plans, schedule, sections, shop drawings, submittals, ventilation system, windows\"\nbs-built_struc_age,Optional,The age of the built structure since construction\nbs-built_struc_set,Optional,\"The characterization of the location of the built structure as high or low human density, e.g., urban, rural\"\nbs-built_struc_type,Optional,A physical structure that is a body or assemblage of bodies in space to form a system capable of supporting loads\nbs-ceil_area,Optional,The area of the ceiling space within the room\nbs-ceil_cond,Optional,\"The physical condition of the ceiling at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-ceil_finish_mat,Optional,\"The type of material used to finish a ceiling, e.g., drywall, mineral fibre, tiles, PVC, plasterboard, metal, fiberglass, stucco, mineral wool/calcium silicate, wood\"\nbs-ceil_struc,Optional,\"The construction format of the ceiling, e.g., wood frame, concrete\"\nbs-ceil_texture,Optional,\"The feel, appearance, or consistency of a ceiling surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-ceil_thermal_mass,Optional,The ability of the ceiling to provide inertia against temperature fluctuations. Generally this means concrete that is exposed. A metal deck that supports a concrete slab will act thermally as long as it is exposed to room air flow\nbs-ceil_type,Optional,\"The type of ceiling according to the ceiling's appearance or construction, e.g., cathedral, dropped, concave, barrel-shaped, coffered, cove, stretched\"\nbs-ceil_water_mold,Optional,\"Signs of the presence of mold or mildew on the ceiling, e.g., presence of mold visible, no presence of mold visible\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cool_syst_id,Optional,The cooling system identifier\nbs-date_last_rain,Optional,The date of the last time it rained\nbs-dew_point,Optional,\"temperature to which a given parcel of humid air must be cooled, at constant barometric pressure, for water vapor to condense into water.\"\nbs-door_comp_type,Optional,\"The composite type of the door, e.g., metal covered, revolving, sliding, telescopic\"\nbs-door_cond,Optional,\"The physical condition of the door, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-door_direct,Optional,\"The direction the door opens, e.g., inward, outward, sideways\"\nbs-door_loc,Optional,\"The relative location of the door in the room, e.g., north, south, east, west\"\nbs-door_mat,Optional,\"The material the door is composed of, e.g., aluminum, cellular PVC, engineered plastic, fiberboard, fiberglass, metal, thermoplastic alloy, vinyl, wood, wood/plastic composite\"\nbs-door_move,Optional,\"The type of movement of the door, e.g., collapsible, folding, revolving, rolling shutter, sliding, swinging\"\nbs-door_size,Optional,The size of the door\nbs-door_type,Optional,\"The type of door material, e.g., composite, metal, wooden\"\nbs-door_type_metal,Optional,\"The type of metal door, e.g., collapsible, corrugated steel, hollow, rolling shutters, steel plate\"\nbs-door_type_wood,Optional,\"The type of wood door, e.g., bettened and ledged, battened, ledged and braced, battened, ledged and framed, battened, ledged, braced and frame, framed and paneled, glashed or sash, flush, louvered, wire gauged\"\nbs-door_water_mold,Optional,\"Signs of the presence of mold or mildew on a door, e.g., presence of mold visible, no presence of mold visible\"\nbs-drawings,Optional,\"The buildings architectural drawings, e.g., operation, as built, construction, bid, design, building navigation map, diagram, sketch\"\nbs-elevator,Optional,The number of elevators within the built structure\nbs-escalator,Optional,The number of escalators within the built structure\nbs-estimated_size,Optional,Estimated size of genome\nbs-exp_duct,Optional,The amount of exposed ductwork in the room\nbs-exp_pipe,Optional,The number of exposed pipes in the room\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ext_door,Optional,The number of exterior doors in the built structure\nbs-ext_wall_orient,Optional,\"The orientation of the exterior wall, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-ext_window_orient,Optional,\"The compass direction the exterior window of the room is facing, e.g., north, south, east, west, northeast, southeast, southwest, northwest\"\nbs-fireplace_type,Optional,\"A firebox with chimney, e.g., gas burning, wood burning\"\nbs-floor_age,Optional,The time period since installment of the carpet or flooring\nbs-floor_area,Optional,The area of the floor space within the room\nbs-floor_cond,Optional,\"The physical condition of the floor at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-floor_count,Optional,\"The number of floors in the building, including basements and mechanical penthouse\"\nbs-floor_finish_mat,Optional,\"The floor covering type; the finished surface that is walked on, e.g., tile, wood strip or parquet, carpet, rug, laminate wood, lineoleum, vinyl composition tile, sheet vinyl, stone, bamboo, cork, terrazo, concrete, none;specify unfinished, sealed, clear finish, paint\"\nbs-floor_struc,Optional,\"Refers to the structural elements and subfloor upon which the finish flooring is installed, e.g., balcony, floating floor, glass floor, raised floor, sprung floor, wood-framed, concrete\"\nbs-floor_thermal_mass,Optional,The ability of the floor to provide inertia against temperature fluctuations\nbs-floor_water_mold,Optional,\"Signs of the presence of mold or mildew in a room, e.g., mold odor, wet floor, water stains, wall discoloration, floor discoloration, ceiling discoloration, peeling paint or wallpaper, bulging walls, condensation\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-freq_cook,Optional,The number of times a meal is cooked per week\nbs-furniture,Optional,\"The types of furniture present in the sampled room, e.g., cabinet, chair, desks\"\nbs-gender_restroom,Optional,\"The gender type of the restroom, e.g., all gender, female, gender neutral, male, male and female, unisex\"\nbs-hall_count,Optional,The total count of hallways and cooridors in the built structure\nbs-handidness,Optional,\"The handidness of the individual sampled, e.g., ambidexterity, left handedness, mixed-handedness, right handedness\"\nbs-heat_deliv_loc,Optional,\"The location of heat delivery within the room, e.g., north, south, east, west\"\nbs-heat_sys_deliv_meth,Optional,\"The method by which the heat is delivered through the system, e.g., conductive, radiant\"\nbs-heat_system_id,Optional,The heating system identifier\nbs-height_carper_fiber,Optional,The average carpet fiber height in the indoor environment\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indoor_surf,Optional,type of indoor surface\nbs-inside_lux,Optional,The recorded value at sampling time (power density)\nbs-int_wall_cond,Optional,\"The physical condition of the wall at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture\"\nbs-last_clean,Optional,\"The last time the floor was cleaned (swept, mopped, vacuumed)\"\nbs-max_occup,Optional,The maximum amount of people allowed in the indoor environment\nbs-mech_struc,Optional,\"Mechanical structure: a moving structure, e.g., subway, coach, carriage, elevator, escalator, boat, train, car, bus\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-number_pets,Optional,The number of pets residing in the sampled space\nbs-number_plants,Optional,The number of plant(s) in the sampling space\nbs-number_resident,Optional,The number of individuals currently occupying in the sampling location\nbs-occup_document,Optional,\"The type of documentation of occupancy, e.g., automated count, estimate, manual count, videos\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pres_animal_insect,Optional,\"The type and number of animals or insects present in the sampling space, e.g., cat 10, dog 2, rodent 100, snake 1\"\nbs-quad_pos,Optional,\"The quadrant position of the sampling room within the building, e.g., north side, west side, south side, east side\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_humidity_out,Optional,The recorded outside relative humidity value at the time of sampling\nbs-rel_samp_loc,Optional,\"The sampling location within the train car, e.g., edge of car, center of car, under a seat\"\nbs-room_air_exch_rate,Optional,The rate at which outside air replaces indoor air in a given space\nbs-room_architec_elem,Optional,\"The unique details and component parts that, together, form the architecture of a distinguisahable space within a built structure\"\nbs-room_condt,Optional,\"The condition of the room at the time of sampling, e.g., new, visible wear, needs repair, damaged, rupture, visible signs of mold/mildew\"\nbs-room_connected,Optional,\"List of rooms connected to the sampling room by a doorway, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, office, stairwell\"\nbs-room_count,Optional,The total count of rooms in the built structure including all room types\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-room_door_dist,Optional,Distance between doors (meters) in the hallway between the sampling room and adjacent rooms\nbs-room_door_share,Optional,\"List of room(s) (room number, room name) sharing a door with the sampling room\"\nbs-room_hallway,Optional,\"List of room(s) (room number, room name) located in the same hallway as sampling room\"\nbs-room_loc,Optional,\"The position of the room within the building, e.g., corner room, interior room, exterior wall\"\nbs-room_moist_dam_hist,Optional,The history of moisture damage or mold in the past 12 months. Number of events of moisture damage or mold observed\nbs-room_net_area,Optional,The net floor area of sampling room. Net area excludes wall thicknesses\nbs-room_occup,Optional,Count of room occupancy at time of sampling\nbs-room_samp_pos,Optional,\"The horizontal sampling position in the room relative to architectural elements, e.g., north corner, south corner, west corner, east corner, northeast corner, northwest corner, southeast corner, southwest corner, center\"\nbs-room_type,Optional,\"The main purpose or activity of the sampling room. A room is any distinguishable space within a structure, e.g., attic, bathroom, closet, conference room, elevator, examining room, hallway, kitchen, mail room, private office, open office, stairwell,restroom, lobby, vestibule, mechanical or electrical room, data center, laboratory_wet, laboratory_dry, gymnasium, natatorium, auditorium, lockers, cafe, warehouse\"\nbs-room_vol,Optional,Volume of sampling room\nbs-room_wall_share,Optional,\"List of room(s) (room number, room name) sharing a wall with the sampling room\"\nbs-room_window_count,Optional,Number of windows in the room\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_sort_meth,Optional,method by which samples are sorted\nbs-samp_time_out,Optional,The recent and long term history of outside sampling\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_weather,Optional,\"The weather on the sampling day, e.g., clear sky, cloudy, foggy, hail, rain, snow, sleet, sunny, windy\"\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_use,Optional,\"The seasons the space is occupied, e.g., spring, summer, fall, winter\"\nbs-shad_dev_water_mold,Optional,\"Signs of the presence of mold or mildew on the shading device, e.g., presence of mold visible, no presence of mold visible\"\nbs-shading_device_cond,Optional,\"The physical condition of the shading device at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-shading_device_loc,Optional,\"The location of the shading device in relation to the built structure, e.g., exterior, interior\"\nbs-shading_device_mat,Optional,The material the shading device is composed of\nbs-shading_device_type,Optional,\"The type of shading device, e.g., bahama shutters, exterior roll blind, gambrel awning, hood awning, porchroller awning, sarasota shutters, slatted aluminum, solid aluminum awning, sun screen, tree, trellis, venetian awning\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-specific,Optional,\"The building specifications, e.g., operation, as built, construction, bid, design, photos\"\nbs-specific_humidity,Optional,\"The mass of water vapour in a unit mass of moist air, usually expressed as grams of vapour per kilogram of air, or, in air conditioning, as grains per pound\"\nbs-substructure_type,Optional,substructure or under building is that largely hidden section of the building which is built off the foundations to the ground floor level\nbs-surf_air_cont,Optional,contaminant identified on surface\nbs-surf_humidity,Optional,surfaces: water activity as a function of air and material moisture\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_moisture,Optional,water held on a surface\nbs-surf_moisture_ph,Optional,pH measurement of surface\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-temp_out,Optional,The recorded temperature value at sampling time outside\nbs-train_line,Optional,\"The subway line name, e.g., red, green, orange\"\nbs-train_stat_loc,Optional,\"The train station collection location, e.g., south station above ground, south station underground, south station amtrak, forest hills, riverside\"\nbs-train_stop_loc,Optional,\"The train stop collection location, e.g., end, mid, downtown\"\nbs-vis_media,Optional,\"The building visual media, e.g., photos, videos, commonly of the building, site context (adjacent buildings, vegetation, terrain, streets), interiors, equipment, 3D scans\"\nbs-wall_area,Optional,The total area of the sampled room's walls\nbs-wall_const_type,Optional,\"The building class of the wall defined by the composition of the building elements and fire-resistance rating, e.g., frame construction, joisted masonry, light noncombustible, masonry noncombustible, modified fire resistive, fire resistive\"\nbs-wall_finish_mat,Optional,\"The material utilized to finish the outer most layer of the wall, e.g., plaster, gypsum plaster, veneer plaster, gypsum board, tile, terrazzo, stone facing, acoustical treatment, wood, metal, masonry\"\nbs-wall_height,Optional,The average height of the walls in the sampled room\nbs-wall_loc,Optional,\"The relative location of the wall within the room, e.g., north, south, east, west\"\nbs-wall_surf_treatment,Optional,\"The surface treatment of interior wall, e.g., painted, wall paper, no treatment, paneling, stucco, fabric\"\nbs-wall_texture,Optional,\"The feel, appearance, or consistency of a wall surface, e.g., crows feet, crows-foot stomp, double skip, hawk and trowel, knockdown, popcorn, orange peel, rosebud stomp, Santa-Fe texture, skip trowel, smooth, stomp knockdown, swirl\"\nbs-wall_thermal_mass,Optional,The ability of the wall to provide inertia against temperature fluctuations. Generally this means concrete or concrete block that is either exposed or covered only with paint\nbs-wall_water_mold,Optional,\"Signs of the presence of mold or mildew on a wall, e.g., presence of mold visible, no presence of mold visible\"\nbs-water_feat_size,Optional,The size of the water feature\nbs-water_feat_type,Optional,\"The type of water feature present within the building being sampled, e.g., fountain, pool, standing feature, stream, waterfall\"\nbs-weekday,Optional,\"The day of the week when sampling occurred, e.g., monday, tuesday, wednesday, thursday, friday, saturday, sunday\"\nbs-window_cond,Optional,\"The physical condition of the window at the time of sampling, e.g., damaged, needs repair, new, rupture, visible wear\"\nbs-window_cover,Optional,\"The type of window covering, e.g., blinds, curtains, none\"\nbs-window_horiz_pos,Optional,\"The horizontal position of the window on the wall, e.g., left, middle, right\"\nbs-window_loc,Optional,\"The relative location of the window within the room, e.g., north, south, east, west\"\nbs-window_mat,Optional,\"The type of material used to finish a window, e.g., clad, fiberglass, metal, vinyl, wood\"\nbs-window_open_freq,Optional,The number of times windows are opened per week\nbs-window_size,Optional,The window's length and width\nbs-window_status,Optional,\"Defines whether the windows were open or closed during environmental testing, e.g., closed, open\"\nbs-window_type,Optional,\"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window\"\nbs-window_vert_pos,Optional,\"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high\"\nbs-window_water_mold,Optional,\"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.food-animal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_origin,Required,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_prod_char,Required,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-intended_consumer,Required,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-animal_am_dur,Optional,The duration of time (days) that the antimicrobial was administered to the food animal\nbs-animal_am_freq,Optional,The frequency per day that the antimicrobial was adminstered to the food animal\nbs-animal_am_route,Optional,The route by which the antimicrobial is adminstered into the body of the food animal\nbs-animal_am_use,Optional,The prescribed intended use of or the condition treated by the antimicrobial given to the food animal by any route of administration\nbs-animal_body_cond,Optional,\"Body condition scoring is a production management tool used to evaluate overall health and nutritional needs of a food animal, e.g., normal, over conditioned, under conditioned\"\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_sex,Optional,\"The sex and reproductive status of the food animal, e.g., castrated female, castrated male, intact female, intact male\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_source_age,Optional,\"The age of the food source host organim. Depending on the type of host organism, age may be more appropriate to report in days, weeks, or years\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.food-farm.env.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-biotic_regm,Required,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-chem_administration,Required,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-adjacent_environment,Optional,Description of the environmental system or features that are adjacent to the sampling site. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-air_flow_impede,Optional,\"Presence of objects in the area that would influence or impede air flow through the air filter, e.g., obstructed, unobstructed\"\nbs-air_pm_concen,Optional,\"concentration of substances that remain suspended in the air, and comprise mixtures of organic and inorganic substances (PM10 and PM2.5); can report multiple PM's by entering numeric values preceded by name of PM\"\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-anim_water_method,Optional,Description of the equipment or method used to distribute water to livestock. This field accepts terms listed under water delivery equipment (http://opendata.inra.fr/EOL/EOL_0001653). Multiple terms can be separated by pipes\nbs-animal_diet,Optional,\"If the isolate is from a food animal, the type of diet eaten by the food animal. Please list the main food staple and the setting, if appropriate. For a list of acceptable animal feed terms or categories, please see http://www.feedipedia.org. Multiple terms may apply and can be separated by pipes. Food product for animal covers foods intended for consumption by domesticated animals. Consult http://purl.obolibrary.org/obo/FOODON_03309997. If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes. If the proper descriptor is not listed please use text to describe the food product type\"\nbs-animal_feed_equip,Optional,Description of the feeding equipment used for livestock. This field accepts terms listed under feed delivery (http://opendata.inra.fr/EOL/EOL_0001757). Multiple terms can be separated by one or more pipes\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-crop_yield,Optional,Amount of crop produced per unit or area of land\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-date_extr_weath,Optional,\"Date of unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_equip_san,Optional,Method used to sanitize growing and harvesting equipment including type and concentration of sanitizing solution and frequency of sanitization\nbs-farm_equip_san_freq,Optional,\"The number of times farm equipment is cleaned. Frequency of cleaning might be on a daily basis, weekly, monthly, quarterly or annually\"\nbs-farm_equip_shared,Optional,\"List of planting, growing or harvesting equipment shared with other farms\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-fertilizer_date,Optional,\"Date of administration of soil amendment or fertilizer. Multiple terms may apply and can be separated by pipes, listing in reverse chronological order\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_harvest_proc,Optional,\"A harvesting process is a process which takes in some food material from an individual or community of plant or animal organisms in a given context and time, and outputs a precursor or consumable food product. This may include a part of an organism or the whole, and may involve killing the organism\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_medium,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). The name of the medium used to grow the microorganism\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_height,Optional,the height of subject\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_length,Optional,the length of subject\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-humidity,Optional,\"amount of water vapour in the air, at the time of sampling\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_part_maturity,Optional,A description of the stage of development of a plant or plant part based on maturity or ripeness. This field accepts terms listed under degree of plant maturity (http://purl.obolibrary.org/obo/FOODON_03530050)\nbs-plant_reprod_crop,Optional,\"Plant reproductive part used in the field during planting to start the crop, e.g., plant cutting, pregerminated seed, ratoon, seed, seedling, whole mature plant\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-salinity,Optional,salinity measurement\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_store_dur,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season,Optional,The season when sampling occurred. Any of the four periods into which the year is divided by the equinoxes and solstices. This field accepts terms listed under season (http://purl.obolibrary.org/obo/NCIT_C94729)\nbs-season_humidity,Optional,Average humidity of the region throughout the growing season\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-soil_conductivity,Optional,Conductivity of soil at time of sampling\nbs-soil_cover,Optional,\"Description of the material covering the sampled soil. This field accepts terms under ENVO:00010483, environmental material\"\nbs-soil_ph,Optional,The pH of soil at time of sampling\nbs-soil_porosity,Optional,Porosity of soil or deposited sediment is volume of voids divided by the total volume of sample\nbs-soil_temp,Optional,Temperature of soil at the time of sampling\nbs-soil_texture_class,Optional,\"One of the 12 soil texture classes use to describe soil texture based on the relative proportion of different grain sizes of mineral particles [sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um)] in a soil, e.g., clay, clay loam, loam, loamy sand, sand, sandy clay, sandy clay loam, sandy loam, silt, silty clay, silty clay loam, silt loam\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-solar_irradiance,Optional,the amount of solar energy that arrives at a specific area of a surface during a specific time interval\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tillage,Optional,note method(s) used for tilling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-ventilation_rate,Optional,ventilation rate of the system in the sampled premises\nbs-ventilation_type,Optional,ventilation system used in the sampled premises\nbs-water_frequency,Optional,Number of water delivery events within a given period of time\nbs-water_ph,Optional,\"The pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid\"\nbs-water_source_adjac,Optional,Description of the environmental features that are adjacent to the farm water source. This field accepts terms under ecosystem (http://purl.obolibrary.org/obo/ENVO_01001110) and human construction (http://purl.obolibrary.org/obo/ENVO_00000070). Multiple terms can be separated by pipes\nbs-water_source_shared,Optional,\"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing\"\nbs-wind_direction,Optional,wind direction is the direction from which a wind originates\nbs-wind_speed,Optional,speed of wind measured at the time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.food-human.foods.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cons_food_stor_dur,Optional,\"The storage duration of the food commodity by the consumer, prior to onset of illness or sample collection. Indicate the timepoint written in ISO 8601 format\"\nbs-cons_food_stor_temp,Optional,\"Temperature at which food commodity was stored by the consumer, prior to onset of illness or sample collection\"\nbs-cons_purch_date,Optional,The date a food product was purchased by consumer\nbs-cons_qty_purchased,Optional,The quantity of food purchased by consumer\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-ferm_chem_add,Optional,Any chemicals that are added to the fermentation process to achieve the desired final product\nbs-ferm_chem_add_perc,Optional,The amount of chemical added to the fermentation process\nbs-ferm_headspace_oxy,Optional,The amount of headspace oxygen in a fermentation vessel\nbs-ferm_medium,Optional,\"The growth medium used for the fermented food fermentation process, which supplies the required nutrients. Usually this includes a carbon and nitrogen source, water, micronutrients and chemical additives\"\nbs-ferm_ph,Optional,The pH of the fermented food fermentation process\nbs-ferm_rel_humidity,Optional,The relative humidity of the fermented food fermentation process\nbs-ferm_temp,Optional,The temperature of the fermented food fermentation process\nbs-ferm_time,Optional,The time duration of the fermented food fermentation process\nbs-ferm_vessel,Optional,The type of vessel used for containment of the fermentation\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_qual,Optional,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-microb_start,Optional,Any type of microorganisms used in food production. This field accepts terms listed under live organisms for food production (http://purl.obolibrary.org/obo/FOODON_0344453)\nbs-microb_start_count,Optional,\"Total cell count of starter culture per gram, volume or area of sample and the method that was used for the enumeration (e.g. qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-microb_start_inoc,Optional,The amount of starter culture used to inoculate a new batch\nbs-microb_start_prep,Optional,Information about the protocol or method used to prepare the starter inoculum\nbs-microb_start_source,Optional,\"The source from which the microbial starter culture was sourced. If commercially supplied, list supplier\"\nbs-microb_start_taxid,Optional,\"Please include Genus species and strain ID, if known of microorganisms used in food production. For complex communities, pipes can be used to separate two or more microbes\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_source_mat_cat,Optional,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Optional,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Optional,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-serovar_or_serotype,Optional,A characterization of a cell or microorganism based on the antigenic properties of the molecules on its surface. Indicate the name of a serovar or serotype of interest. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-spikein_amr,Optional,\"Qualitative description of a microbial response to antimicrobial agents. Bacteria may be susceptible or resistant to a broad range of antibiotic drugs or drug classes, with several intermediate states or phases. This field accepts terms under antimicrobial phenotype (http://purl.obolibrary.org/obo/ARO_3004299)\"\nbs-spikein_antibiotic,Optional,\"Antimicrobials used in research study to assess effects of exposure on microbiome of a specific site. Please list antimicrobial, common name and/or class and concentration used for spike-in\"\nbs-spikein_count,Optional,\"Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. The method that was used for the enumeration (e.g., qPCR, atp, mpn, etc.) should also be provided, e.g., total prokaryotes; 3.5e7 cells per ml; qPCR\"\nbs-spikein_growth_med,Optional,\"A liquid or gel containing nutrients, salts, and other factors formulated to support the growth of microorganisms, cells, or plants (National Cancer Institute Thesaurus). A growth medium is a culture medium which has the disposition to encourage growth of particular bacteria to the exclusion of others in the same growth environment. In this case, list the culture medium used to propagate the spike-in bacteria during preparation of spike-in inoculum. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the spike in growth media\"\nbs-spikein_metal,Optional,Heavy metals used in research study to assess effects of exposure on microbiome of a specific site. Please list heavy metals and concentration used for spike-in\nbs-spikein_org,Optional,Taxonomic information about the spike-in organism(s). This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_serovar,Optional,Taxonomic information about the spike-in organism(s) at the serovar or serotype level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-spikein_strain,Optional,Taxonomic information about the spike-in organism(s) at the strain level. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.food-prod.facility.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-coll_site_geo_feat,Required,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-food_contact_surf,Required,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_product_qual,Required,\"Descriptors for describing food visually or via other senses, which is useful for tasks like food inspection where little prior knowledge of how the food came to be is available. Some terms like \"\"food (frozen)\"\" are both a quality descriptor and the output of a process. This field accepts terms listed under food product by quality (http://purl.obolibrary.org/obo/FOODON_00002454)\"\nbs-food_product_type,Required,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-ifsac_category,Required,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-samp_source_mat_cat,Required,This is the scientific role or category that the subject organism or material has with respect to an investigation. This field accepts terms listed under specimen source material category (http://purl.obolibrary.org/obo/GENEPIO_0001237 or http://purl.obolibrary.org/obo/OBI_0100051)\nbs-samp_stor_device,Required,The container used to store the sample. This field accepts terms listed under container (http://purl.obolibrary.org/obo/NCIT_C43186). If the proper descriptor is not listed please use text to describe the storage device\nbs-samp_stor_media,Required,\"The liquid that is added to the sample collection device prior to sampling. If the sample is pre-hydrated, indicate the liquid media the sample is pre-hydrated with for storage purposes. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the sample storage media\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-air_temp,Optional,temperature of the air at the time of sampling\nbs-area_samp_size,Optional,\"The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected\"\nbs-avg_occup,Optional,Daily average occupancy of room. Indicate the number of person(s) daily occupying the sampling room\nbs-bacterial_density,Optional,\"Number of bacteria in sample, as defined by bacteria density (http://purl.obolibrary.org/obo/GENEPIO_0000043)\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-cult_result,Optional,\"Any result of a bacterial culture experiment reported as a binary assessment, e.g., absent, active, inactive, negative, no, present, positive, yes\"\nbs-cult_result_org,Optional,Taxonomic information about the cultured organism(s)\nbs-cult_target,Optional,The target microbial analyte in terms of investigation scope. This field accepts terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also accepts identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy\nbs-dietary_claim_use,Optional,\"These descriptors are used either for foods intended for special dietary use as defined in 21 CFR 105 or for foods that have special characteristics indicated in the name or labeling. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510023). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the most prominent dietary claim or use\"\nbs-enrichment_protocol,Optional,The microbiological workflow or protocol followed to test for the presence or enumeration of the target microbial analyte(s). Please provide a PubMed or DOI reference for published protocols\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_allergen_label,Optional,A label indication that the product contains a recognized allergen. This field accepts terms listed under dietary claim or use (http://purl.obolibrary.org/obo/FOODON_03510213)\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_cooking_proc,Optional,\"The transformation of raw food by the application of heat. This field encourages terms listed under food cooking (http://purl.obolibrary.org/obo/FOODON_03450002), e.g., food blanching [FOODON:03470175]\"\nbs-food_dis_point,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_dis_point_city,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that refers to a distribution point along the food chain. This field accepts terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448). Reference: Adam Diamond, James Barham. Moving Food Along the Value Chain: Innovations in Regional Food Distribution. U.S. Dept. of Agriculture, Agricultural Marketing Service. Washington, DC. March 2012. http://dx.doi.org/10.9752/MS045.03-2012\"\nbs-food_ingredient,Optional,\"In this field, please list individual ingredients for multi-component food [FOODON:00002501] and simple foods that is not captured in food_type. Please use terms that are present in FoodOn. Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors\"\nbs-food_name_status,Optional,A datum indicating that use of a food product name is regulated in some legal jurisdiction. This field accepts terms listed under food product name legal status (http://purl.obolibrary.org/obo/FOODON_03530087)\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_capacity,Optional,The maximum number of product units within a package\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_prior_contact,Optional,\"The material the food contacted (e.g., was processed in) prior to packaging. This field accepts terms listed under material of contact prior to food packaging (http://purl.obolibrary.org/obo/FOODON_03530077). If the proper descriptor is not listed please use text to describe the material of contact prior to food packaging\"\nbs-food_prod_char,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, dairy, beef\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_trace_list,Optional,\"The FDA is proposing to establish additional traceability recordkeeping requirements (beyond what is already required in existing regulations) for persons who manufacture, process, pack, or hold foods the Agency has designated for inclusion on the Food Traceability List. The Food Traceability List (FTL) identifies the foods for which the additional traceability records described in the proposed rule would be required. The term \"\"\"\"Food Traceability List\"\"\"\" (FTL) refers not only to the foods specifically listed (https://www.fda.gov/media/142303/download), but also to any foods that contain listed foods as ingredients, e.g., tropical tree fruits\"\nbs-food_trav_mode,Optional,A descriptor for the method of movement of food commodity along the food distribution system. This field accepts terms listed under travel mode (http://purl.obolibrary.org/obo/GENEPIO_0001064). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_trav_vehic,Optional,A descriptor for the mobile machine which is used to transport food commodities along the food distribution system. This field accepts terms listed under vehicle (http://purl.obolibrary.org/obo/ENVO_01000604). If the proper descrptor is not listed please use text to describe the mode of travel. Multiple terms can be separated by one or more pipes\nbs-food_treat_proc,Optional,\"Used to specifically characterize a food product based on the treatment or processes applied to the product or any indexed ingredient. The processes include adding, substituting or removing components or modifying the food or component, e.g., through fermentation. Multiple values can be assigned. This fields accepts terms listed under food treatment process (http://purl.obolibrary.org/obo/FOODON_03460111)\"\nbs-freq_clean,Optional,\"The number of times the sample location is cleaned, e.g., daily, weekly, monthly, quarterly, annually\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-haccp_term,Optional,Hazard Analysis Critical Control Points (HACCP) food safety terms; This field accepts terms listed under HACCP guide food safety term (http://purl.obolibrary.org/obo/FOODON_03530221)\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-hygienic_area,Optional,The subdivision of areas within a food production facility according to hygienic requirements. This field accepts terms listed under hygienic food production area (http://purl.obolibrary.org/obo/ENVO). Please add a term that most accurately indicates the hygienic area your sample was taken from according to the definitions provided\nbs-indoor_surf,Optional,type of indoor surface\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-lot_number,Optional,A distinctive alpha-numeric identification code assigned by the manufacturer or distributor to a specific quantity of manufactured material or product within a batch. The submitter should provide lot number of the item followed by the item name for which the lot number was provided\nbs-microb_cult_med,Optional,\"A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms. This field accepts terms listed under microbiological culture medium (http://purl.obolibrary.org/obo/MICRO_0000067). If the proper descriptor is not listed please use text to describe the culture medium\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-num_samp_collect,Optional,The number of samples collected during the current sampling event\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-part_plant_animal,Optional,\"The anatomical part of the organism being involved in food production or consumption; e.g., a carrot is the root of the plant (root vegetable). This field accepts terms listed under part of plant or animal (http://purl.obolibrary.org/obo/FOODON_03420116)\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-prod_label_claims,Optional,\"Labeling claims containing descriptors such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free\"\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-repository,Optional,\"the name of the institution where the sample or DNA extract is held or \"\"sample not available\"\" if the sample was used in its entirety for analysis or otherwise not retained\"\nbs-room_dim,Optional,\"The length, width and height of sampling room\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_collect_method,Optional,The method employed for collecting the sample\nbs-samp_floor,Optional,\"The floor of the building, where the sampling room is located, e.g., 1st floor, 2nd floor, basement, lobby\"\nbs-samp_loc_condition,Optional,\"The condition of the sample location at the time of sampling, e.g., damaged, new, rupture, visible signs of mold-mildew, visible weariness repair\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_pooling,Optional,\"Physical combination of several instances of like material, e.g., RNA extracted from samples or dishes of cell cultures into one big aliquot of cells. Please provide a short description of the samples that were pooled\"\nbs-samp_rep_biol,Optional,Measurements of biologically distinct samples that show biological variation\nbs-samp_rep_tech,Optional,Repeated measurements of the same sample that show independent measures of the noise associated with the equipment and the protocols\nbs-samp_room_id,Optional,Sampling room number. This ID should be consistent with the designations on the building floor plans\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_surf_moisture,Optional,\"Degree of water held on a sampled surface, e.g., intermittent moisture, not present, submerged\"\nbs-samp_transport_cont,Optional,\"Container in which the sample was stored during transport. Indicate the location name, e.g., bottle, cooler, glass vial, plastic vial, vendor supplied container\"\nbs-samp_transport_dur,Optional,The duration of time from when the sample was collected until processed. Indicate the duration for which the sample was stored written in ISO 8601 format\nbs-samp_transport_temp,Optional,\"Temperature at which sample was transported, e.g., -20 or 4 degree Celsius\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-ster_meth_samp_room,Optional,\"The method used to sterilize the sampling room. This field accepts terms listed under electromagnetic radiation (http://purl.obolibrary.org/obo/ENVO_01001026). If the proper descriptor is not listed, please use text to describe the sampling room sterilization method. Multiple terms can be separated by pipes\"\nbs-study_design,Optional,Epidemiological or omics reseach design context that this biosample was used in.\nbs-study_inc_dur,Optional,Sample incubation duration if unpublished or unvalidated method is used. Indicate the timepoint written in ISO 8601 format\nbs-study_inc_temp,Optional,Sample incubation temperature if unpublished or unvalidated method is used\nbs-study_timecourse,Optional,\"For time-course research studies involving samples of the food commodity, indicate the total duration of the time-course study\"\nbs-study_tmnt,Optional,\"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes\"\nbs-subspecf_gen_lin,Optional,\"Information about the genetic distinctness of the lineage (eg., biovar, serovar)\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.host-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_blood_press_diast,Optional,\"resting diastolic blood pressureof the host, measured as mm mercury\"\nbs-host_blood_press_syst,Optional,\"resting systolic blood pressure of the host, measured as mm mercury\"\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.human-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-amniotic_fluid_color,Optional,specification of the color of the amniotic fluid sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-blood_blood_disord,Optional,history of blood disorders; can include multiple disorders\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diet_last_six_month,Optional,\"specification of major diet changes in the last six months, if yes the change should be specified\"\nbs-drug_usage,Optional,any drug used by subject and the frequency of usage; can include multiple drugs used\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-foetal_health_stat,Optional,\"specification of foetal health status, should also include abortion\"\nbs-gestation_state,Optional,specification of the gestation state\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_hiv_stat,Optional,\"HIV status of subject, if yes HAART initiation status should also be indicated as [YES or NO]\"\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-kidney_disord,Optional,history of kidney disorders; can include multiple disorders\nbs-maternal_health_stat,Optional,specification of the maternal health status\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_throat_disord,Optional,history of nose-throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pet_farm_animal,Optional,\"specification of presence of pets or farm animals in the environment of subject, if yes the animals should be specified; can include multiple animals present\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pulmonary_disord,Optional,history of pulmonary disorders; can include multiple disorders\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-smoker,Optional,specification of smoking status\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-study_complt_stat,Optional,\"specification of study completion status, if no the reason should be specified\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-travel_out_six_month,Optional,specification of the countries travelled in the last six months; can include multiple travels\nbs-twin_sibling,Optional,specification of twin sibling presence\nbs-urine_collect_meth,Optional,specification of urine collection method\nbs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders\nbs-weight_loss_3_month,Optional,\"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.human-gut.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gastrointest_disord,Optional,history of gastrointestinal tract disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-liver_disord,Optional,history of liver disorders; can include multiple disorders\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-special_diet,Optional,specification of special diet; can include multiple special diets\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.human-oral.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nose_mouth_teeth_throat_disord,Optional,history of nose/mouth/teeth/throat disorders; can include multiple disorders\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_last_toothbrush,Optional,specification of the time since last toothbrushing\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.human-skin.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-dermatology_disord,Optional,history of dermatology disorders; can include multiple disorders\nbs-dominant_hand,Optional,dominant hand of the subject\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-time_since_last_wash,Optional,specification of the time since last wash\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.human-vaginal.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-birth_control,Optional,specification of birth control medication used\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-douche,Optional,date of most recent douche\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethnicity,Optional,ethnicity of the subject\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gynecologic_disord,Optional,history of gynecological disorders; can include multiple disorders\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_mass_index,Optional,\"body mass index of the host, calculated as weight/(height)squared\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_body_temp,Optional,core body temperature of the host when sample was collected\nbs-host_diet,Optional,\"type of diet depending on the sample for animals omnivore, herbivore etc., for humans high-fat, meditteranean etc.; can include multiple diet types\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_last_meal,Optional,content of last meal and time since feeding; can include multiple values\nbs-host_occupation,Optional,most frequent job performed by subject\nbs-host_phenotype,Optional,\nbs-host_pulse,Optional,\"resting pulse of the host, measured as beats per minute\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-hrt,Optional,\"whether subject had hormone replacement theraphy, and if yes start date\"\nbs-hysterectomy,Optional,specification of whether hysterectomy was performed\nbs-ihmc_medication_code,Optional,can include multiple medication codes\nbs-medic_hist_perform,Optional,whether full medical history was collected\nbs-menarche,Optional,date of most recent menstruation\nbs-menopause,Optional,date of onset of menopause\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pregnancy,Optional,date due of pregnancy\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sexual_act,Optional,current sexual partner and frequency of sex\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-urogenit_disord,Optional,\"history of urogenital disorders, can include multiple disorders\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.hydrocarbon-cores.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-hcr_temp,Required,Original temperature of the hydrocarbon resource\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-sulfate_fw,Required,Original sulfate concentration in the hydrocarbon resource\nbs-temp,Required,temperature of the sample at time of sampling\nbs-vfa_fw,Required,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-owc_tvdss,Optional,Depth of the original oil water contact (OWC) zone (average) (m TVDSS)\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-permeability,Optional,Measure of the ability of a hydrocarbon resource to allow fluids to pass through it. Additional information: https://en.wikipedia.org/wiki/Permeability_(earth_sciences)\nbs-ph,Optional,pH measurement\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_md,Optional,\"In non deviated well, measured depth is equal to the true vertical depth, TVD (TVD=TVDSS plus the reference or datum it refers to). In deviated wells, the MD is the length of trajectory of the borehole measured from the same reference or datum. Common datums used are ground level (GL), drilling rig floor (DF), rotary table (RT), kelly bushing (KB) and mean sea level (MSL). If \"\"other\"\" is specified, please propose entry in \"\"additional info\"\" field\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_tvdss,Optional,\"Depth of the sample i.e. The vertical distance between the sea level and the sampled position in the subsurface. Depth can be reported as an interval for subsurface samples e.g., 1325.75-1362.25 m\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sr_dep_env,Optional,\"Source rock depositional environment (https://en.wikipedia.org/wiki/Source_rock), e.g., lacustine, fluvioldeltaic, fluviomarine, marine\"\nbs-sr_geol_age,Optional,\"Geological age of source rock (Additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-sr_kerog_type,Optional,\"Origin of kerogen. Type I: Algal (aquatic), Type II: planktonic and soft plant material (aquatic or terrestrial), Type III: terrestrial woody/ fibrous plant material (terrestrial), Type IV: oxidized recycled woody debris (terrestrial) (additional information: https://en.wikipedia.org/wiki/Kerogen), e.g., type I, type II, type III, type IV\"\nbs-sr_lithology,Optional,\"Lithology of source rock (https://en.wikipedia.org/wiki/Source_rock), e.g., clastic, carbonate, coal, biosilicieous\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.hydrocarbon-fluids.swabs.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-add_recov_method,Required,\"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}\"\nbs-api,Required,\"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API\"\nbs-basin_name,Required,\"Name of the basin, e.g. Campos\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-hc_produced,Required,\"Main hydrocarbon type produced from resource, e.g., oil, gas-condensate, gas, bitumen, coalbed methane\"\nbs-hcr,Required,\"Main hydrocarbon resource type. The term Hydrocarbon Resource is defined as a natural environmental feature containing large amounts of hydrocarbons at high concentrations potentially suitable for commercial exploitation, e.g., oil reservoir, gas reservoir, oil sand, coalbed, shale, tight oil reservoir, tight gas reservoir\"\nbs-iwf,Required,\"Proportion of the produced fluids derived from injected water at the time of sampling, e.g., 87%\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-nitrate,Required,concentration of nitrate\nbs-samp_collect_point,Required,\"Sampling point on the asset where sample was collected, e.g., well, test well, drilling rig, wellhead, separator, storage tank\"\nbs-samp_mat_type,Required,\"The type of material from which the sample was obtained. For the Hydrocarbon package, samples include types like core, rock trimmings, drill cuttings, piping section, coupon, pigging debris, solid deposit, produced fluid, produced water, injected water, swabs, etc. For the Food Package, samples are usually categorized as food, body products or tissues, or environmental material. This field accepts terms listed under environmental specimen (http://purl.obolibrary.org/obo/GENEPIO_0001246)\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-sulfate,Required,concentration of sulfate\nbs-sulfide,Required,concentration of sulfide\nbs-temp,Required,temperature of the sample at time of sampling\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-water_cut,Required,Current amount of water (%) in a produced fluid stream; or the average of the combined streams\nbs-additional_info,Optional,Information that doesn't fit anywhere else\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-ammonium,Optional,concentration of ammonium\nbs-aromatics_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-asphaltenes_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-benzene,Optional,Concentration of benzene in the sample\nbs-biocide_admin,Optional,List of biocides (commercial name of product and supplier) and date of administration\nbs-biocide_admin_method,Optional,\"Method of biocide administration (dose, frequency, duration, time elapsed between last biociding and sampling), e.g., 150 mg/l; weekly; 4 hr; 3 days\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-calcium,Optional,concentration of calcium\nbs-chem_treat_method,Optional,\"Method of chemical administration, dose, frequency, duration, time elapsed between administration and sampling, e.g., 50 mg/l; twice a week; 1 hr; 0 days\"\nbs-chem_treatment,Optional,\"List of chemical compounds administered upstream the sampling location where sampling occurred, e.g., glycols, H2S scavenger, corrosion and scale inhibitors, demulsifiers, and other production chemicals. The commercial name of the product and name of the supplier should be provided. The date of administration should also be included\"\nbs-chloride,Optional,concentration of chloride\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depos_env,Optional,\"Main depositional environment (https://en.wikipedia.org/wiki/Depositional_environment), e.g., continental - alluvial, continental - aeolian, continental - fluvial, continental - lacustrine, transitional - deltaic, transitional - tidal, transitional - lagoonal, transitional - beach, transitional - lake, marine - shallow, marine - deep, marine - reef, other - evaporite, other - glacial, other - volcanic\"\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_iron,Optional,Concentration of dissolved iron in the sample\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_oxygen_fluid,Optional,\"Concentration of dissolved oxygen in the oil field produced fluids as it contributes to oxgen-corrosion and microbial activity, e.g. Mic\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-ethylbenzene,Optional,Concentration of ethylbenzene in the sample\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-field,Optional,\"Name of the hydrocarbon field, e.g., Albacora\"\nbs-hcr_fw_salinity,Optional,Original formation water salinity (prior to secondary recovery e.g. Waterflooding) expressed as TDS\nbs-hcr_geol_age,Optional,\"Geological age of hydrocarbon resource (additional info: https://en.wikipedia.org/wiki/Period_(geology)), e.g., archean, cambrian, carboniferous, cenozoic, cretaceous, devonian, jurassic, mesozoic, neogene, ordovician, paleogene, paleozoic, permian, precambrian, proterozoic, silurian, triassic\"\nbs-hcr_pressure,Optional,Original pressure of the hydrocarbon resource\nbs-hcr_temp,Optional,Original temperature of the hydrocarbon resource\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-iw_bt_date_well,Optional,Injection water breakthrough date per well following a secondary and/or tertiary recovery\nbs-lithology,Optional,\"Hydrocarbon resource main lithology (Additional information: http://petrowiki.org/Lithology_and_rock_type_determination), e.g., basement, chalk, chert, coal, conglomerate, diatomite, dolomite, limestone, sandstone, shale, siltstone, volcanic\"\nbs-magnesium,Optional,concentration of magnesium\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrite,Optional,concentration of nitrite\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_count_qpcr_info,Optional,\"If qpcr was used for the cell count, the target gene name, the primer sequence and the cycling conditions should also be provided, e.g., 16S rrna; FWD:ACGTAGCTATGACGT REV:GTGCTAGTCGAGTAC; initial denaturation:90C_5min; denaturation:90C_2min; annealing:52C_30 sec; elongation:72C_30 sec; 90 C for 1 min; final elongation:72C_5min; 30 cycles\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pour_point,Optional,\"Temperature at which a liquid becomes semi solid and loses its flow characteristics. In crude oil a high pour point is generally associated with a high paraffin content, typically found in crude deriving from a larger proportion of plant material (source: https://en.wikipedia.org/wiki/pour_point)\"\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-prod_rate,Optional,\"Oil and/or gas production rates per well, e.g., 524 m3/day\"\nbs-prod_start_date,Optional,Date of field's first production\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-reservoir_name,Optional,\"Name of the reservoir, e.g., Carapebus\"\nbs-resins_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_loc_corr_rate,Optional,\"Metal corrosion rate is the speed of metal deterioration due to environmental conditions. As environmental conditions change corrosion rates change accordingly. Therefore, long term corrosion rates are generally more informative than short term rates and for that reason they are preferred during reporting. In the case of suspected MIC, corrosion rate measurements at the time of sampling might provide insights into the involvement of certain microbial community members in MIC as well as potential microbial interplays\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_preserv,Optional,\"Preservative added to the sample, e.g. Rnalater, alcohol, formaldehyde. Where appropriate include volume added, e.g. Rnalater; 2 ml\"\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_subtype,Optional,\"Name of sample sub-type, e.g., oil phase, water phase, biofilm, not applicable\"\nbs-samp_transport_cond,Optional,\"Sample transport duration (in days or hrs) and temperature the sample was exposed to (e.g., 5.5 days; 20C)\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-samp_well_name,Optional,\"Name of the well where sample was taken, e.g., BXA1123\"\nbs-saturates_pc,Optional,\"Saturate, Aromatic, Resin and Asphaltene (SARA) is an analysis method that divides crude oil components according to their polarizability and polarity. There are three main methods to obtain SARA results. The most popular one is known as the Iatroscan TLC-FID and is referred to as IP-143 (source: https://en.wikipedia.org/wiki/Saturate,_aromatic,_resin_and_asphaltene)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate_fw,Optional,Original sulfate concentration in the hydrocarbon resource\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-tan,Optional,Total Acid Number (TAN) is a measurement of acidity that is determined by the amount of potassium hydroxide in milligrams that is needed to neutralize the acids in one gram of oil. It is an important quality measurement of crude oil. (source: https://en.wikipedia.org/wiki/Total_acid_number)\nbs-toluene,Optional,Concentration of toluene in the sample\nbs-tot_iron,Optional,Concentration of total iron in the sample\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-tot_sulfur,Optional,Concentration of total sulfur in the sample\nbs-tvdss_of_hcr_press,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original pressure was measured, e.g., 1578 m\"\nbs-tvdss_of_hcr_temp,Optional,\"True vertical depth subsea (TVDSS) of the hydrocarbon resource where the original temperature was measured, e.g., 1345 m\"\nbs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample\nbs-vfa_fw,Optional,Original volatile fatty acid concentration in the hydrocarbon resource\nbs-viscosity,Optional,\"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)\"\nbs-water_prod_rate,Optional,\"Water production rates per well, e.g., 987 m3/day\"\nbs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)\nbs-xylene,Optional,Concentration of xylene in the sample\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.microbial.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tot_carb,Optional,total carbon content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.miscellaneous.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ammonium,Optional,concentration of ammonium\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.plant-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-air_temp_regm,Optional,\"information about treatment involving an exposure to varying temperatures; should include the temperature, treatment duration, interval and total experimental duration; can include different temperature regimens\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-ances_data,Optional,\"Information about either pedigree or other ancestral information description, e.g., parental variety in case of mutant or selection, A/3*B (meaning [(A x B) x B] x B)\"\nbs-antibiotic_regm,Optional,\"information about treatment involving antibiotic administration; should include the name of antibiotic, amount administered, treatment duration, interval and total experimental duration; can include multiple antibiotic regimens\"\nbs-biol_stat,Optional,\"The level of genome modification, e.g., wild, natural, semi-natural, inbred line, breeder's line, hybrid, clonal selection, mutant\"\nbs-biotic_regm,Optional,\"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_mutagen,Optional,\"treatment involving use of mutagens; should include the name of mutagen, amount administered, treatment duration, interval and total experimental duration; can include multiple mutagen regimens\"\nbs-climate_environment,Optional,treatment involving an exposure to a particular climate; can include multiple climates\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-cult_root_med,Optional,\"Name or reference for the hydroponic or in vitro culture rooting medium; can be the name of a commonly used medium or reference to a specific medium, e.g., Murashige and Skoog medium. If the medium has not been formally published, use the rooting medium descriptors\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fertilizer_regm,Optional,\"information about treatment involving the use of fertilizers; should include the name fertilizer, amount administered, treatment duration, interval and total experimental duration; can include multiple fertilizer regimens\"\nbs-fungicide_regm,Optional,\"information about treatment involving use of fungicides; should include the name of fungicide, amount administered, treatment duration, interval and total experimental duration; can include multiple fungicide regimens\"\nbs-gaseous_environment,Optional,\"use of conditions with differing gaseous environments; should include the name of gaseous compound, amount administered, treatment duration, interval and total experimental duration; can include multiple gaseous environment regimens\"\nbs-genetic_mod,Optional,\"Genetic modifications of the genome of an organism, which may occur naturally by spontaneous mutation, or be introduced by some experimental means, e.g. specification of a transgene or the gene knocked-out or details of transient transfection\"\nbs-gravity,Optional,\"information about treatment involving use of gravity factor to study various types of responses in presence, absence or modified levels of gravity; can include multiple treatments\"\nbs-growth_facil,Optional,\"Type of facility where the sampled plant was grown; controlled vocabulary: growth chamber, open top chamber, glasshouse, experimental garden, field. Alternatively use Crop Ontology (CO) terms, see http://www.cropontology.org/ontology/CO_715/Crop%20Research\"\nbs-growth_habit,Optional,\"Characteristic shape, appearance or growth form of a plant species, e.g., erect, semi-erect, spreading, prostrate\"\nbs-growth_hormone_regm,Optional,\"information about treatment involving use of growth hormones; should include the name of growth hormone, amount administered, treatment duration, interval and total experimental duration; can include multiple growth hormone regimens\"\nbs-herbicide_regm,Optional,\"information about treatment involving use of herbicides; information about treatment involving use of growth hormones; should include the name of herbicide, amount administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_genotype,Optional,\nbs-host_height,Optional,the height of subject\nbs-host_length,Optional,the length of subject\nbs-host_life_stage,Optional,description of host life stage\nbs-host_phenotype,Optional,\nbs-host_subspecf_genlin,Optional,\"Information about the genetic distinctness of the host organism below the subspecies level e.g., serovar, serotype, biotype, ecotype, variety, cultivar, or any relevant genetic typing schemes like Group I plasmid. Subspecies should not be recorded in this term, but in the NCBI taxonomy. Supply both the lineage name and the lineage rank separated by a colon, e.g., biovar:abc123\"\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-host_wet_mass,Optional,measurement of wet mass\nbs-humidity_regm,Optional,\"information about treatment involving an exposure to varying degree of humidity; information about treatment involving use of growth hormones; should include amount of humidity administered, treatment duration, interval and total experimental duration; can include multiple regimens\"\nbs-light_regm,Optional,\"Information about treatment(s) involving exposure to light, including both light intensity and quality\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-mineral_nutr_regm,Optional,\"information about treatment involving the use of mineral supplements; should include the name of mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple mineral nutrient regimens\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-non_mineral_nutr_regm,Optional,\"information about treatment involving the exposure of plant to non-mineral nutrient such as oxygen, hydrogen or carbon; should include the name of non-mineral nutrient, amount administered, treatment duration, interval and total experimental duration; can include multiple non-mineral nutrient regimens\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pesticide_regm,Optional,\"information about treatment involving use of insecticides; should include the name of pesticide, amount administered, treatment duration, interval and total experimental duration; can include multiple pesticide regimens\"\nbs-ph_regm,Optional,information about treatment involving exposure of plants to varying levels of pH of the growth media; can include multiple regimen\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_product,Optional,\"substance produced by the plant, where the sample was obtained from\"\nbs-plant_sex,Optional,\"Sex of the reproductive parts on the whole plant, e.g., androdioecious, androecious, androgynous, androgynomonoecious, andromonoecious, bisexual, dichogamous, diclinous, dioecious, gynodioecious, gynoecious, gynomonoecious, hermaphroditic, imperfect, monoclinous, monoecious, perfect, polygamodioecious, polygamomonoecious, polygamous, protandrous, protogynous, subandroecious, subdioecious, subgynoecious, synoecious, trimonoecious, trioecious, unisexual\"\nbs-plant_struc,Optional,\"Name of plant structure the sample was obtained from; for Plant Ontology (PO) (v releases/2017-12-14) terms, see http://purl.bioontology.org/ontology/PO, e.g., petiole epidermis (PO_0000051). If an individual flower is sampled, the sex of it can be recorded here\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-radiation_regm,Optional,\"information about treatment involving exposure of plant or a plant part to a particular radiation regimen; should include the radiation type, amount or intensity administered, treatment duration, interval and total experimental duration; can include multiple radiation regimens\"\nbs-rainfall_regm,Optional,information about treatment involving an exposure to a given amount of rainfall; can include multiple regimens\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-root_cond,Optional,\"Relevant rooting conditions such as field plot size, sowing density, container dimensions, number of plants per container\"\nbs-root_med_carbon,Optional,\"Source of organic carbon in the culture rooting medium, e.g., sucrose\"\nbs-root_med_macronutr,Optional,\"Measurement of the culture rooting medium macronutrients (N,P, K, Ca, Mg, S), e.g., KH2PO4 (170mg/L)\"\nbs-root_med_micronutr,Optional,\"Measurement of the culture rooting medium micronutrients (Fe, Mn, Zn, B, Cu, Mo), e.g., H3BO3 (6.2mg/L)\"\nbs-root_med_ph,Optional,\"pH measurement of the culture rooting medium, e.g., 5.5\"\nbs-root_med_regl,Optional,\"Growth regulators in the culture rooting medium such as cytokinins, auxins, gybberellins, abscisic acid, e.g., 0.5 mg/L NAA\"\nbs-root_med_solid,Optional,\"Specification of the solidifying agent in the culture rooting medium, e.g., agar\"\nbs-root_med_suppl,Optional,\"Organic supplements of the culture rooting medium, such as vitamins, amino acids, organic acids, antibiotics activated charcoal, e.g., nicotinic acid (0.5 mg/L)\"\nbs-salt_regm,Optional,\"information about treatment involving use of salts as supplement to liquid and soil growth media; should include the name of salt, amount administered, treatment duration, interval and total experimental duration; can include multiple salt regimens\"\nbs-samp_capt_status,Optional,\"Reason for the sample, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, farm sample, market sample\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_dis_stage,Optional,\"Stage of the disease at the time of sample collection, e.g., dissemination, growth and reproduction, infection, inoculation, penetration\"\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_environment,Optional,\"treatment involving an exposure to a particular season (e.g. winter, summer, rabi, rainy etc.)\"\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-standing_water_regm,Optional,\"treatment involving an exposure to standing water during a plant's life span, types can be flood water or standing water; can include multiple regimens\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used\nbs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens\nbs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.sediment.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-methane,Optional,methane (gas) amount or concentration at the time of sampling\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-particle_class,Optional,\"particles are classified, based on their size, into six general categories:clay, silt, sand, gravel, cobbles, and boulders; should include amount of particle preceded by the name of the particle type; can include multiple values\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-porosity,Optional,porosity of deposited sediment is volume of voids divided by the total volume of sample\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-sediment_type,Optional,information about the sediment type based on major constituents\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sodium,Optional,sodium concentration\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_carb,Optional,total carbon content\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-turbidity,Optional,turbidity measurement\nbs-water_content,Optional,water content measurement\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.soil.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-agrochem_addition,Optional,\"addition of fertilizers, pesticides, etc. - amount and time of applications\"\nbs-al_sat,Optional,aluminum saturation (esp. for tropical soils)\nbs-al_sat_meth,Optional,reference or method used in determining Al saturation\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-annual_precpt,Optional,\"The average of all annual precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-annual_temp,Optional,Mean annual temperature\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-crop_rotation,Optional,\"whether or not crop is rotated, and if yes, rotation schedule\"\nbs-cur_land_use,Optional,present state of sample site\nbs-cur_vegetation,Optional,\"vegetation classification from one or more standard classification systems, or agricultural crop\"\nbs-cur_vegetation_meth,Optional,reference or method used in vegetation classification\nbs-drainage_class,Optional,drainage classification from a standard system such as the USDA system\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-extreme_event,Optional,unusual physical events that may have affected microbial populations\nbs-fao_class,Optional,soil classification from the FAO World Reference Database for Soil Resources\nbs-fire,Optional,historical and/or physical evidence of fire\nbs-flooding,Optional,historical and/or physical evidence of flooding\nbs-heavy_metals,Optional,heavy metals present and concentrationsany drug used by subject and the frequency of usage; can include multiple heavy metals and concentrations\nbs-heavy_metals_meth,Optional,reference or method used in determining heavy metals\nbs-horizon_meth,Optional,reference or method used in determining the horizon\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-link_addit_analys,Optional,\nbs-link_class_info,Optional,link to digitized soil maps or other soil classification information\nbs-link_climate_info,Optional,link to climate resource\nbs-local_class,Optional,soil classification based on local soil classification system\nbs-local_class_meth,Optional,reference or method used in determining the local soil classification\nbs-microbial_biomass,Optional,\"the part of the organic matter in the soil that constitutes living microorganisms smaller than 5-10 \u00b5m. IF you keep this, you would need to have correction factors used for conversion to the final units, which should be mg C (or N)/kg soil).\"\nbs-microbial_biomass_meth,Optional,reference or method used in determining microbial biomass\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-ph,Optional,pH measurement\nbs-ph_meth,Optional,reference or method used in determining pH\nbs-pool_dna_extracts,Optional,were multiple DNA extractions mixed? how many?\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-previous_land_use,Optional,previous land use and dates\nbs-previous_land_use_meth,Optional,reference or method used in determining previous land use and dates\nbs-profile_position,Optional,cross-sectional position in the hillslope where sample was collected.sample area position in relation to surrounding areas\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity_meth,Optional,reference or method used in determining salinity\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-season_precpt,Optional,\"The average of all seasonal precipitation values known, or an estimated equivalent value derived by such methods as regional indexes or Isohyetal maps\"\nbs-season_temp,Optional,Mean seasonal temperature\nbs-sieving,Optional,collection design of pooled samples and/or sieve size and amount of sample sieved\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-slope_aspect,Optional,\"the direction a slope faces. While looking down a slope use a compass to record the direction you are facing (direction or degrees); e.g., NW or 315\u00b0. This measure provides an indication of sun and wind exposure that will influence soil temperature and evapotranspiration.\"\nbs-slope_gradient,Optional,commonly called \u201cslope.\u201d The angle between ground surface and a horizontal line (in percent). This is the direction that overland water would flow. This measure is usually taken with a hand level meter or clinometer.\nbs-soil_horizon,Optional,specific layer in the land area which measures parallel to the soil surface and possesses physical characteristics which differ from the layers above and beneath\nbs-soil_text_measure,Optional,\"the relative proportion of different grain sizes of mineral particles in a soil, as described using a standard system; express as % sand (50 um to 2 mm), silt (2 um to 50 um), and clay (<2 um) with textural name (e.g., silty clay loam) optional.\"\nbs-soil_texture_meth,Optional,reference or method used in determining soil texture\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-soil_type_meth,Optional,reference or method used in determining soil series name or other lower-level classification\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-tillage,Optional,note method(s) used for tilling\nbs-tot_n_meth,Optional,reference or method used in determining the total N\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_nitro_cont_meth,Optional,Reference or method used in determining the total nitrogen\nbs-tot_org_c_meth,Optional,reference or method used in determining total organic C\nbs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content\nbs-water_content,Optional,water content measurement\nbs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.symbiont-associated.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host_dependence,Required,\"Type of host dependence for the symbiotic host organism to its host., e.g., facultative, obligate\"\nbs-host_life_stage,Required,description of host life stage\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-sym_life_cycle_type,Required,\"Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle, e.g., complex life cycle, simple life cycle\"\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-association_duration,Optional,Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gravidity,Optional,\"whether or not subject is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_body_habitat,Optional,original body habitat where the sample was obtained from\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_cellular_loc,Optional,\"The localization of the symbiotic host organism within the host from which it was sampled, e.g., intracellular, extracellular\"\nbs-host_color,Optional,the color of host\nbs-host_common_name,Optional,\"The natural language (non-taxonomic) name of the host organism, e.g., mouse\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_dry_mass,Optional,measurement of dry mass\nbs-host_family_relationship,Optional,\nbs-host_genotype,Optional,\nbs-host_growth_cond,Optional,literature reference giving growth conditions of the host\nbs-host_height,Optional,the height of subject\nbs-host_infra_specific_name,Optional,taxonomic information subspecies level\nbs-host_infra_specific_rank,Optional,\"taxonomic rank information below subspecies level, such as variety, form, rank etc.\"\nbs-host_length,Optional,the length of subject\nbs-host_number,Optional,Number of symbiotic host individuals pooled at the time of collection\nbs-host_of_host_coinf,Optional,\"The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism, e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple co-infecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont\"\nbs-host_of_host_disease,Optional,\"List of diseases with which the host of the symbiotic host organism has been diagnosed; can include multiple diagnoses. The value of the field depends on host; for humans the terms should be chosen from the DO (Human Disease Ontology) at https://www.disease-ontology.org, non-human host diseases are free text\"\nbs-host_of_host_env_loc,Optional,\"For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/)\"\nbs-host_of_host_env_med,Optional,\"Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of 'environmental material' (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart)\"\nbs-host_of_host_fam_rel,Optional,Familial relationship of the host of the symbiotic host organisms to other hosts of symbiotic host organism in the same study; can include multiple relationships\nbs-host_of_host_geno,Optional,Observed genotype of the host of the symbiotic host organism\nbs-host_of_host_gravid,Optional,\"Whether or not the host of the symbiotic host organism is gravid, and if yes date due or date post-conception, specifying which is used\"\nbs-host_of_host_infname,Optional,Taxonomic name information of the host of the symbiotic host organism below subspecies level\nbs-host_of_host_infrank,Optional,\"Taxonomic rank information about the host of the symbiotic host organism below subspecies level, such as variety, form, rank\"\nbs-host_of_host_name,Optional,Common name of the host of the symbiotic host organism\nbs-host_of_host_pheno,Optional,\"Phenotype of the host of the symbiotic host organism. For phenotypic quality ontology (PATO) terms, see http://purl.bioontology.org/ontology/pato\"\nbs-host_of_host_sub_id,Optional,\"A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14\"\nbs-host_of_host_taxid,Optional,NCBI taxon id of the host of the symbiotic host organism\nbs-host_of_host_totmass,Optional,\"Total mass of the host of the symbiotic host organism at collection, the unit depends on the host\"\nbs-host_phenotype,Optional,\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_shape,Optional,morphological shape of host\nbs-host_specificity,Optional,\"Level of specificity of symbiont-host interaction, e.g., family-specific, generalist, genus-specific, species-specific\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_substrate,Optional,the growth substrate of the host\nbs-host_symbiont,Optional,\"The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_tot_mass,Optional,\"total mass of the host at collection, the unit depends on host\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-mode_transmission,Optional,\"The process through which the symbiotic host organism entered the host from which it was sampled, e.g., horizontal:castrator, horizontal:directly transmitted, horizontal:micropredator, horizontal:parasitoid, horizontal:trophically transmitted, horizontal:vector transmitted, vertical\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-route_transmission,Optional,\"Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission), e.g., environmental:faecal-oral, transplacental, vector-borne:vector penetration\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_sol,Optional,\"Solution within which sample was stored, if any\"\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-symbiont_host_role,Optional,\"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-type_of_symbiosis,Optional,\"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.wastewater.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-biochem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chem_oxygen_dem,Optional,a measure of the relative oxygen-depletion effect of a waste contaminant\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-efficiency_percent,Optional,percentage of volatile solids removed from the anaerobic digestor\nbs-emulsions,Optional,\"amount or concentration of substances such as paints, adhesives, mayonnaise, hair colorants, emulsified oils, etc.; can include multiple emulsion types\"\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-gaseous_substances,Optional,\"amount or concentration of substances such as hydrogen sulfide, carbon dioxide, methane, etc.; can include multiple substances\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-indust_eff_percent,Optional,percentage of industrial effluents received by wastewater treatment plant\nbs-inorg_particles,Optional,\"concentration of particles such as sand, grit, metal particles, ceramics, etc.; can include multiple particles\"\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_particles,Optional,\"concentration of particles such as faeces, hairs, food, vomit, paper fibers, plant material, humus, etc.\"\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-ph,Optional,pH measurement\nbs-phosphate,Optional,concentration of phosphate\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-pre_treatment,Optional,the process of pre-treatment removes materials that can be easily collected from the raw wastewater\nbs-primary_treatment,Optional,the process to produce both a generally homogeneous liquid capable of being treated biologically and a sludge that can be separately treated or processed\nbs-reactor_type,Optional,\"anaerobic digesters can be designed and engineered to operate using a number of different process configurations, as batch or continuous, mesophilic, high solid or low solid, and single stage or multistage\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_salinity,Optional,\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-secondary_treatment,Optional,the process for substantially degrading the biological content of the sewage\nbs-sewage_type,Optional,type of wastewater treatment plant as municipial or industrial\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-sludge_retent_time,Optional,the time activated sludge remains in reactor\nbs-sodium,Optional,sodium concentration\nbs-soluble_inorg_mat,Optional,\"concentration of substances such as ammonia, road-salt, sea-salt, cyanide, hydrogen sulfide, thiocyanates, thiosulfates, etc.\"\nbs-soluble_org_mat,Optional,\"concentration of substances such as urea, fruit sugars, soluble proteins, drugs, pharmaceuticals, etc.\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-suspend_solids,Optional,\"concentration of substances including a wide variety of material, such as silt, decaying plant and animal matter, etc,; can include multiple substances\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tertiary_treatment,Optional,the process providing a final treatment stage to raise the effluent quality before it is discharged to the receiving environment\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_phosphate,Optional,total amount or concentration of phosphate\nbs-wastewater_type,Optional,\"the origin of wastewater such as human waste, rainfall, storm drains, etc.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.MIUVIG.water.6.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-depth,Required,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Required,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-source_uvig,Required,Type of dataset from which the UViG was obtained\nbs-virus_enrich_appr,Required,\"Approach used to enrich the sample for viruses, if any. If more than one approach was used, include multiple \u2018virus_enrich_appr\u2019 fields.\"\nbs-alkalinity,Optional,\"alkalinity, the ability of a solution to neutralize acids to the equivalence point of carbonate or bicarbonate\"\nbs-alkalinity_method,Optional,Method used for alkalinity measurement\nbs-alkyl_diethers,Optional,concentration of alkyl diethers\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-aminopept_act,Optional,measurement of aminopeptidase activity\nbs-ammonium,Optional,concentration of ammonium\nbs-atmospheric_data,Optional,measurement of atmospheric data; can include multiple data\nbs-bac_prod,Optional,bacterial production in the water column measured by isotope uptake\nbs-bac_resp,Optional,measurement of bacterial respiration in the water column\nbs-bacteria_carb_prod,Optional,measurement of bacterial carbon production\nbs-biomass,Optional,\"amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements\"\nbs-biotic_relationship,Optional,Free-living or from host (define relationship)\nbs-bishomohopanol,Optional,concentration of bishomohopanol\nbs-bromide,Optional,concentration of bromide\nbs-calcium,Optional,concentration of calcium\nbs-carb_nitro_ratio,Optional,ratio of amount or concentrations of carbon to nitrogen\nbs-chem_administration,Optional,\"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603\"\nbs-chloride,Optional,concentration of chloride\nbs-chlorophyll,Optional,concentration of chlorophyll\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-conduc,Optional,electrical conductivity of water\nbs-density,Optional,density of sample\nbs-diether_lipids,Optional,concentration of diether lipids; can include multiple types of diether lipids\nbs-diss_carb_dioxide,Optional,concentration of dissolved carbon dioxide\nbs-diss_hydrogen,Optional,concentration of dissolved hydrogen\nbs-diss_inorg_carb,Optional,dissolved inorganic carbon concentration\nbs-diss_inorg_nitro,Optional,concentration of dissolved inorganic nitrogen\nbs-diss_inorg_phosp,Optional,concentration of dissolved inorganic phosphorus\nbs-diss_org_carb,Optional,concentration of dissolved organic carbon\nbs-diss_org_nitro,Optional,dissolved organic nitrogen concentration measured as; total dissolved nitrogen - NH4 - NO3 - NO2\nbs-diss_oxygen,Optional,concentration of dissolved oxygen\nbs-down_par,Optional,visible waveband radiance and irradiance measurements in the water column\nbs-elev,Optional,The elevation of the sampling site as measured by the vertical distance from mean sea level.\nbs-estimated_size,Optional,Estimated size of genome\nbs-experimental_factor,Optional,Variable aspect of experimental design\nbs-fluor,Optional,raw or converted fluorescence of water\nbs-glucosidase_act,Optional,measurement of glucosidase activity\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_taxid,Optional,\"NCBI taxonomy ID of the host, e.g. 9606\"\nbs-light_intensity,Optional,measurement of light intensity\nbs-magnesium,Optional,concentration of magnesium\nbs-mean_frict_vel,Optional,measurement of mean friction velocity\nbs-mean_peak_frict_vel,Optional,measurement of mean peak friction velocity\nbs-misc_param,Optional,\"any other measurement performed or parameter collected, that is not listed here\"\nbs-n_alkanes,Optional,concentration of n-alkanes; can include multiple n-alkanes\nbs-neg_cont_type,Optional,\"The substance or equipment used as a negative control in an investigation, e.g., distilled water, phosphate buffer, empty collection device, empty collection tube, DNA-free PCR mix, sterile swab, sterile syringe\"\nbs-nitrate,Optional,concentration of nitrate\nbs-nitrite,Optional,concentration of nitrite\nbs-nitro,Optional,concentration of nitrogen (total)\nbs-omics_observ_id,Optional,\"A unique identifier of the omics-enabled observatory (or comparable time series) your data derives from. This identifier should be provided by the OMICON ontology; if you require a new identifier for your time series, contact the ontology's developers. Information is available here: https://github.com/GLOMICON/omicon. This field is only applicable to records which derive from an omics time-series or observatory.\"\nbs-org_carb,Optional,concentration of organic carbon\nbs-org_matter,Optional,concentration of organic matter\nbs-org_nitro,Optional,concentration of organic nitrogen\nbs-organism_count,Optional,\"total count of any organism per gram or volume of sample,should include name of organism followed by count; can include multiple organism counts\"\nbs-oxy_stat_samp,Optional,oxygenation status of sample\nbs-part_org_carb,Optional,concentration of particulate organic carbon\nbs-part_org_nitro,Optional,concentration of particulate organic nitrogen\nbs-pathogenicity,Optional,To what is the entity pathogenic\nbs-perturbation,Optional,\"type of perturbation, e.g. chemical administration, physical disturbance, etc., coupled with time that perturbation occurred; can include multiple perturbation types\"\nbs-petroleum_hydrocarb,Optional,concentration of petroleum hydrocarbon\nbs-ph,Optional,pH measurement\nbs-phaeopigments,Optional,concentration of phaeopigments; can include multiple phaeopigments\nbs-phosphate,Optional,concentration of phosphate\nbs-phosplipid_fatt_acid,Optional,concentration of phospholipid fatty acids; can include multiple values\nbs-photon_flux,Optional,measurement of photon flux\nbs-pos_cont_type,Optional,\"The substance, mixture, product, or apparatus used to verify that a process which is part of an investigation delivers a true positive\"\nbs-potassium,Optional,concentration of potassium\nbs-pressure,Optional,\"pressure to which the sample is subject, in atmospheres\"\nbs-primary_prod,Optional,measurement of primary production\nbs-redox_potential,Optional,\"redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-salinity,Optional,salinity measurement\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-samp_store_dur,Optional,\nbs-samp_store_loc,Optional,\nbs-samp_store_temp,Optional,\nbs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction\nbs-silicate,Optional,concentration of silicate\nbs-size_frac,Optional,\"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer\"\nbs-size_frac_low,Optional,Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample\nbs-size_frac_up,Optional,Refers to the mesh/pore size used to retain the sample. Materials smaller than the size threshold are excluded from the sample\nbs-sodium,Optional,sodium concentration\nbs-soluble_react_phosp,Optional,concentration of soluble reactive phosphorus\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-sulfate,Optional,concentration of sulfate\nbs-sulfide,Optional,concentration of sulfide\nbs-suspend_part_matter,Optional,concentration of suspended particulate matter\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-tidal_stage,Optional,stage of tide\nbs-tot_depth_water_col,Optional,measurement of total depth of water column\nbs-tot_diss_nitro,Optional,\"total dissolved nitrogen concentration, reported as nitrogen, measured by: total dissolved nitrogen = NH4 + NO3NO2 + dissolved organic nitrogen\"\nbs-tot_inorg_nitro,Optional,total inorganic nitrogen content\nbs-tot_nitro,Optional,total nitrogen content of the sample\nbs-tot_part_carb,Optional,total particulate carbon content\nbs-tot_phosp,Optional,\"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus\"\nbs-turbidity,Optional,turbidity measurement\nbs-water_current,Optional,measurement of magnitude and direction of flow within a fluid\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Metagenome.environmental.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-ref_biomaterial,Optional,Primary publication or genome report\nbs-rel_to_oxygen,Optional,\"Is this organism an aerobe, anaerobe? Please note that aerobic and anaerobic are valid descriptors for microbial environments, eg, aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe, missing, not applicable, not collected, not provided, restricted access\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-samp_mat_process,Optional,Processing applied to the sample during or after isolation\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-source_material_id,Optional,\"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Microbe.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-isolation_source,\"At least one field required. Group: At least one required: Group \"\"Source\",\"At least one required: Group \"\"Source\"\". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-sample_type,Required,\"Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-biomaterial_provider,Optional,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-env_broad_scale,Optional,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-genotype,Optional,observed genotype\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-identified_by,Optional,name of the taxonomist who identified the specimen\nbs-lab_host,Optional,\"Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained, e.g., Escherichia coli DH5a, or Homo sapiens HeLa cells\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-mating_type,Optional,\nbs-passage_history,Optional,Number of passages and passage method\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-serotype,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serogroup. e.g. serotype=\"\"H1N1\"\" in Influenza A virus CY098518.\"\nbs-serovar,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serotype. Sometimes used as species identifier in bacteria with shaky taxonomy, e.g. Leptospira, serovar saopaolo S76607 (65357 in Entrez).\"\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Model.organism.animal.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-breed,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". breed name - chiefly used in domesticated animals or plants\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-age,\"At least one field required. Group: At least one required: Group \"\"Age/stage\",\"At least one required: Group \"\"Age/stage\"\". age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees\"\nbs-dev_stage,\"At least one field required. Group: At least one required: Group \"\"Age/stage\",\"At least one required: Group \"\"Age/stage\"\". Developmental stage at the time of sampling.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-sex,Required,physical sex of sampled organism\nbs-tissue,Required,Type of tissue the sample was taken from.\nbs-biomaterial_provider,Optional,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-birth_date,Optional,\nbs-birth_location,Optional,\nbs-breeding_history,Optional,\nbs-breeding_method,Optional,\nbs-cell_line,Optional,Name of the cell line.\nbs-cell_subtype,Optional,\nbs-cell_type,Optional,Type of cell of the sample or from which the sample was obtained.\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-death_date,Optional,\nbs-disease,Optional,\"list of diseases diagnosed; can include multiple diagnoses. the value of the field depends on host; for humans the terms should be chosen from DO (Disease Ontology), free text for non-human. For DO terms, please see http://gemina.svn.sourceforge.net/viewvc/gemina/trunk/Gemina/ontologies/gemina_symptom.obo?view=log\"\nbs-disease_stage,Optional,stage of disease at the time of sampling.\nbs-genotype,Optional,observed genotype\nbs-growth_protocol,Optional,\nbs-health_state,Optional,Health or disease status of sample at time of collection\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-phenotype,Optional,\"Phenotype of sampled organism. For Phenotypic quality Ontology (PATO) (v1.269) terms, please see http://bioportal.bioontology.org/visualize/44601\"\nbs-sample_type,Optional,\"Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly\"\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.\nbs-stud_book_number,Optional,\nbs-treatment,Optional,\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.OneHealthEnteric.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-collected_by,Required,Name of persons or institute who collected the sample\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-purpose_of_sampling,Required,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-source_type,Required,\"Controlled vocabulary describing the isolation_source. Choose the best fit term: Human, Animal, Food, Environmental, Other, e.g., Food\"\nbs-strain,Required,microbial or eukaryotic strain name\nbs-animal_env,Optional,\"The environment from which the animal sample was taken, e.g., veterinary clinic\"\nbs-animal_intrusion,Optional,\"Identification of animals intruding on the sample or sample site including invertebrates (such as pests or pollinators) and vertebrates (such as wildlife or domesticated animals). This field encourages terms under organism (http://purl.obolibrary.org/obo/NCIT_C14250). This field also encourages identification numbers from NCBI under https://www.ncbi.nlm.nih.gov/taxonomy. Multiple terms can be separated by pipes, e.g., large flies\"\nbs-biocide_used,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; that inhibits the growth, reproduction, and activity of organisms, including fungal cells; decreases the number of fungi or pests present; deters microbial growth and degradation of other ingredients in the formulation. Indicate the biocide used on the location where the sample was taken. Multiple terms can be separated by pipes, e.g., Quaternary ammonium compound|SterBac\"\nbs-building_setting,Optional,location (geography) where a building is set\nbs-coll_site_geo_feat,Optional,\"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]\"\nbs-cult_isol_date,Optional,\"A culture isolation date is a date-time entity marking the end of a process in which a sample yields a positive result for the target microbial analyte(s) in the form of an isolated colony or colonies, e.g., 5/24/2020\"\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-env_broad_scale,Optional,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-env_local_scale,Optional,\"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]\"\nbs-env_medium,Optional,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]\nbs-env_monitoring_zone,Optional,\"An environmental monitoring zone is a formal designation as part of an environmental monitoring program, in which areas of a food production facility are categorized, commonly as zones 1-4, based on likelihood or risk of foodborne pathogen contamination. This field accepts entries of zones 1-4, e.g., Zone 1\"\nbs-extr_weather_event,Optional,\"Unusual weather events that may have affected microbial populations. Multiple terms can be separated by pipes, listed in reverse chronological order, e.g., hail\"\nbs-facility_type,Optional,\"Establishment details about the type of facility where the sample was taken. This is independent of the specific product(s) within the facility, e.g., manufacturing-processing\"\nbs-farm_equip,Optional,\"List of relevant equipment used for planting, fertilization, harvesting, irrigation, land levelling, residue management, weeding or transplanting during the growing season. This field encourages terms listed under agricultural implement (http://purl.obolibrary.org/obo/AGRO_00000416). Multiple terms can be separated by pipes, e.g., combine harvester [AGRO:00000473]\"\nbs-farm_water_source,Optional,\"Source of water used on the farm for irrigation of crops or watering of livestock, e.g., water well\"\nbs-fertilizer_admin,Optional,\"Type of fertilizer or amendment added to the soil or water for the purpose of improving substrate health and quality for plant growth. This field encourages terms listed under agronomic fertilizer (http://purl.obolibrary.org/obo/AGRO_00002062). Multiple terms may apply and can be separated by pipes, listing in reverse chronological order, e.g., fish emulsion [AGRO:00000082]\"\nbs-food_additive,Optional,\"A substance or substances added to food to maintain or improve safety and freshness, to improve or maintain nutritional value, or improve taste, texture and appearance. This field encourages terms listed under food additive (http://purl.obolibrary.org/obo/FOODON_03412972). Multiple terms can be separated by one or more pipes, but please consider limiting this list to the top 5 ingredients listed in order as on the food label. See also, https://www.fda.gov/food/food-ingredients-packaging/overview-food-ingredients-additives-colors, e.g., xanthan gum [FOODON:03413321]\"\nbs-food_clean_proc,Optional,\"The process of cleaning food to separate other environmental materials from the food source. Multiple terms can be separated by pipes, e.g., rinsed with water|scrubbed with brush\"\nbs-food_contact_surf,Optional,\"The specific container or coating materials in direct contact with the food. Multiple values can be assigned. This field encourages terms listed under food contact surface (http://purl.obolibrary.org/obo/FOODON_03500010), e.g., aluminum surface [FOODON:03500042]\"\nbs-food_contain_wrap,Optional,\"Type of container or wrapping defined by the main container material, the container form, and the material of the liner lids or ends. Also type of container or wrapping by form; prefer description by material first, then by form. This field encourages terms listed under food container or wrapping (http://purl.obolibrary.org/obo/FOODON_03490100), e.g., bottle or jar [FOODON:03490203]\"\nbs-food_industry_class,Optional,\"The US FDA Class is the second of five elements that comprise a FDA product code. This element is directly related to an Industry and designates the food group, source, product, use, pharmacological action, category or animal species of the product. A Class code is more specific than an Industry; for example, the Fishery/Seafood products Industry may contain Classes such as Smoked, Breaded and such, e.g., Coffee, Decaffeinated\"\nbs-food_industry_code,Optional,\"The US FDA Industry Code is the first of five elements that comprise an FDA product code. An Industry code determines the broadest area into which a product falls, e.g., 31 Coffee and Tea\"\nbs-food_origin,Optional,\"A reference to a place on the Earth, by its name or by its geographical location that describes the origin of the food commodity, either in terms of its cultivation or production. This field encourages terms listed under geographic location (http://purl.obolibrary.org/obo/GAZ_00000448), e.g., Thailand\"\nbs-food_pack_integrity,Optional,\"A term label and term id to describe the state of the packing material and text to explain the exact condition. This field encourages terms listed under food packing medium integrity (http://purl.obolibrary.org/obo/FOODON_03530218), e.g., food packing medium compromised [FOODON:00002517]\"\nbs-food_pack_medium,Optional,\"The medium in which the food is packed for preservation and handling or the medium surrounding homemade foods, e.g., peaches cooked in sugar syrup. The packing medium may provide a controlled environment for the food. It may also serve to improve palatability and consumer appeal. This includes edible packing media (e.g. fruit juice), gas other than air (e.g. carbon dioxide), vacuum packed, or packed with aerosol propellant. This field encourages terms under food packing medium (http://purl.obolibrary.org/obo/FOODON_03480020). Multiple terms may apply and can be separated by pipes, e.g., vacuum-packed [FOODON:03480027]\"\nbs-food_preserv_proc,Optional,\"The methods contributing to the prevention or retardation of microbial, enzymatic or oxidative spoilage and thus to the extension of shelf life. This field encourages terms listed under food preservation process (http://purl.obolibrary.org/obo/FOODON_03470107), e.g., food fermentation [FOODON:00001304]\"\nbs-food_processing_method,Optional,\"Methods for processing food, e.g., food (blanched) [FOODON:00002767]\"\nbs-food_prod,Optional,\"Descriptors of the food production system or of the agricultural environment and growing conditions related to the farm production system. This field encourages terms listed under food production (http://purl.obolibrary.org/obo/FOODON_03530206). Multiple terms may apply and can be separated by pipes, e.g., restaurant food preparation process [FOODON:03530110]\"\nbs-food_prod_synonym,Optional,\"Other names by which the food product is known by (e.g., regional or non-English names), e.g., pinot gris\"\nbs-food_product_type,Optional,\"A food product type is a class of food products that is differentiated by its food composition (e.g., single- or multi-ingredient), processing and/or consumption characteristics. This does not include brand name products but it may include generic food dish categories. This field encourages terms under food product type (http://purl.obolibrary.org/obo/FOODON:03400361). For terms related to food product for an animal, consult food product for animal (http://purl.obolibrary.org/obo/FOODON_03309997). If the proper descriptor is not listed please use text to describe the food type. Multiple terms can be separated by one or more pipes, e.g., multi-component food product [FOODON:00002501]\"\nbs-food_quality_date,Optional,\"The date recommended for the use of the product while at peak quality, this date is not a reflection of safety unless used on infant formula this date is not a reflection of safety and is typically labeled on a food product as \"\"\"\"best if used by,\"\"\"\" best by,\"\"\"\" \"\"\"\"use by,\"\"\"\" or \"\"\"\"freeze by\"\"\"\", e.g., 5/24/2020\"\nbs-food_source,Optional,\"Individual organism or category of organisms from which the food product or its major ingredient is derived, e.g., giant tiger prawn [FOODON:03412612]\"\nbs-food_type_processed,Optional,\"Food type processed in facility, e.g., dairy\"\nbs-host,Optional,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_am,Optional,\"The class(es) or name(s) (generic or brand) of the antimicrobial(s) given to the food animal within the last 30 days, e.g., tetracycline [CHEBI:27902]\"\nbs-host_animal_breed,Optional,\"The animal breed of the host organism. Used mainly for animal hosts, e.g., duroc, angus, mixed breed\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_disease,Optional,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-host_group_size,Optional,\"The number of food animals of the same species that are maintained together as a unit, i.e. a herd or flock, e.g., 80\"\nbs-host_housing,Optional,\"Description of the housing system of the livestock. This field encourages terms listed under terrestrial management housing system (http://opendata.inra.fr/EOL/EOL_0001605), e.g., pen [EOL:0001902]\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-host_variety,Optional,\"The variety or cultivar of the host organism; the name given to a particular variety of organism to distinguish it from otherwise similar organisms of the same species, e.g., romaine lettuce; Athena melons; Patio Snacker cucumbers\"\nbs-ifsac_category,Optional,\"The IFSAC food categorization scheme has five distinct levels to which foods can be assigned, depending upon the type of food. First, foods are assigned to one of four food groups (aquatic animals, land animals, plants, and other). Food groups include increasingly specific food categories; dairy, eggs, meat and poultry, and game are in the land animal food group, and the category meat and poultry is further subdivided into more specific categories of meat (beef, pork, other meat) and poultry (chicken, turkey, other poultry). Finally, foods are differentiated by differences in food processing (such as pasteurized fluid dairy products, unpasteurized fluid dairy products, pasteurized solid and semi-solid dairy products, and unpasteurized solid and semi-solid dairy products. An IFSAC food category chart is available from https://www.cdc.gov/foodsafety/ifsac/projects/food-categorization-scheme.html PMID: 28926300, e.g., Plants:Produce:Vegetables:Herbs:Dried Herbs\"\nbs-indoor_surf,Optional,type of indoor surface\nbs-indoor_surf_subpart,Optional,\"The subpart of the object(s) that was swabbed, like a cart, drain, counter, etc., e.g., wheel\"\nbs-intended_consumer,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed, e.g., human\"\nbs-isolate_name_alias,Optional,Other IDs associated with this isolate or strain. Separate with '; ' if more than one\nbs-label_claims,Optional,\"Descriptors of the food production system such as wild caught, free-range, organic, free-range, industrial, hormone-free, antibiotic free, cage free, e.g., wild caught\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-location_in_facility,Optional,\"Name of the location or room in the facility where the sampling occurred, e.g., ripening room\"\nbs-material_condition,Optional,\"Condition of material sampled, e.g., rusted\"\nbs-mechanical_damage,Optional,information about any mechanical damage exerted on the plant; can include multiple damages and sites\nbs-plant_growth_med,Optional,\"Type of the media used for growing sampled plants, e.g., soil [ENVO:00001998]\"\nbs-plant_water_method,Optional,\"Description of the equipment or method used to distribute water to crops. This field encourages terms listed under irrigation process (http://purl.obolibrary.org/obo/AGRO_00000006). Multiple terms can be separated by pipes, e.g., drip irrigation process [AGRO:00000056]\"\nbs-project_name,Optional,\"A concise name that describes the overall project, for example \"\"Analysis of sequences collected from Antarctic soil\"\"\"\nbs-reference_material,Optional,\"Indicates that a standards body or external group asserts this sample is reference material, eg, 'NIST reference material for genome sequencing validation'.\"\nbs-rel_location,Optional,\"Location of sample to other parts of the farm, e.g. under crop plant, near irrigation ditch, from the dirt road, from air above crops, nearby river, e.g., furrow\"\nbs-samp_collect_device,Optional,Method or device employed for collecting sample\nbs-sanitizer_used_postharvest,Optional,\"Substance intended for preventing, neutralizing, destroying, repelling, or mitigating the effects of any pest or microorganism; indicate the sanitizer used during the postharvest cleaning process. Multiple terms can be separated by pipes, e.g., chlorine bleach\"\nbs-sequenced_by,Optional,\"The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention\"\nbs-serotype,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serogroup. e.g. serotype=\"\"H1N1\"\" in Influenza A virus CY098518.\"\nbs-serovar,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serotype. Sometimes used as species identifier in bacteria with shaky taxonomy, e.g. Leptospira, serovar saopaolo S76607 (65357 in Entrez).\"\nbs-soil_type,Optional,soil series name or other lower-level classification\nbs-spec_intended_cons,Optional,\"Food consumer type, human or animal, for which the food product is produced and marketed. This field encourages terms listed under food consumer group (http://purl.obolibrary.org/obo/FOODON_03510136), e.g., infant or toddler as food consumer [FOODON:03510020]\"\nbs-surf_material,Optional,surface materials at the point of sampling\nbs-surf_temp,Optional,temperature of the surface at the time of sampling\nbs-surface_orientation,Optional,\"Describe which surface of the object is swabbed. (e.g underside, top, corner), e.g., underside\"\nbs-upstream_intervention,Optional,\"List any known health-related interventions (prophylactic, therapeutic, and/or metaphylactic) administered to the host, i.e. vaccinations, hormonal patches, antibiotics, injections in feed, etc., e.g., prophylactic/metaphylactic antibiotics\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Pathogen.cl.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-collected_by,Required,Name of persons or institute who collected the sample\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Required,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-genotype,Optional,observed genotype\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_description,Optional,Additional information not included in other defined vocabulary fields\nbs-host_disease_outcome,Optional,\"Final outcome of disease, e.g., death, chronic disease, recovery\"\nbs-host_disease_stage,Optional,Stage of disease at the time of sampling\nbs-host_health_state,Optional,Information regarding health state of the individual sampled at the time of sampling\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-passage_history,Optional,Number of passages and passage method\nbs-pathotype,Optional,\"Some bacterial specific pathotypes (example Eschericia coli - STEC, UPEC)\"\nbs-serotype,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serogroup. e.g. serotype=\"\"H1N1\"\" in Influenza A virus CY098518.\"\nbs-serovar,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serotype. Sometimes used as species identifier in bacteria with shaky taxonomy, e.g. Leptospira, serovar saopaolo S76607 (65357 in Entrez).\"\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-subgroup,Optional,Taxonomy below subspecies; sometimes used in viruses to denote subgroups taken from a single isolate.\nbs-subtype,Optional,\"Used as classifier in viruses (e.g. HIV type 1, Group M, Subtype A).\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Pathogen.env.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-strain,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". microbial or eukaryotic strain name\"\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-collected_by,Required,Name of persons or institute who collected the sample\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Required,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-genotype,Optional,observed genotype\nbs-passage_history,Optional,Number of passages and passage method\nbs-pathotype,Optional,\"Some bacterial specific pathotypes (example Eschericia coli - STEC, UPEC)\"\nbs-serotype,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serogroup. e.g. serotype=\"\"H1N1\"\" in Influenza A virus CY098518.\"\nbs-serovar,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serotype. Sometimes used as species identifier in bacteria with shaky taxonomy, e.g. Leptospira, serovar saopaolo S76607 (65357 in Entrez).\"\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-subgroup,Optional,Taxonomy below subspecies; sometimes used in viruses to denote subgroups taken from a single isolate.\nbs-subtype,Optional,\"Used as classifier in viruses (e.g. HIV type 1, Group M, Subtype A).\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Plant.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". identification or description of the specific individual from which this sample was obtained\"\nbs-cultivar,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". cultivar name - cultivated variety of plant\"\nbs-ecotype,\"At least one field required. Group: At least one required: Group \"\"Organism\",\"At least one required: Group \"\"Organism\"\". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia\"\nbs-age,\"At least one field required. Group: At least one required: Group \"\"Age/stage\",\"At least one required: Group \"\"Age/stage\"\". age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees\"\nbs-dev_stage,\"At least one field required. Group: At least one required: Group \"\"Age/stage\",\"At least one required: Group \"\"Age/stage\"\". Developmental stage at the time of sampling.\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-tissue,Required,Type of tissue the sample was taken from.\nbs-biomaterial_provider,Optional,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-cell_line,Optional,Name of the cell line.\nbs-cell_type,Optional,Type of cell of the sample or from which the sample was obtained.\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-disease,Optional,\"list of diseases diagnosed; can include multiple diagnoses. the value of the field depends on host; for humans the terms should be chosen from DO (Disease Ontology), free text for non-human. For DO terms, please see http://gemina.svn.sourceforge.net/viewvc/gemina/trunk/Gemina/ontologies/gemina_symptom.obo?view=log\"\nbs-disease_stage,Optional,stage of disease at the time of sampling.\nbs-genotype,Optional,observed genotype\nbs-growth_protocol,Optional,\nbs-height_or_length,Optional,measurement of height or length\nbs-isolation_source,Optional,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-phenotype,Optional,\"Phenotype of sampled organism. For Phenotypic quality Ontology (PATO) (v1.269) terms, please see http://bioportal.bioontology.org/visualize/44601\"\nbs-population,Optional,\"for human: ; for plants: filial generation, number of progeny, genetic structure\"\nbs-sample_type,Optional,\"Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly\"\nbs-sex,Optional,physical sex of sampled organism\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-treatment,Optional,\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.SARS-CoV-2.cl.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-collected_by,Required,Name of persons or institute who collected the sample\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-host,Required,\"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-host_disease,Required,\"Name of relevant disease, e.g. Salmonella gastroenteritis. Controlled vocabulary, http://bioportal.bioontology.org/ontologies/1009 or http://www.ncbi.nlm.nih.gov/mesh\"\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-antiviral_treatment_agent,Optional,What was the antiviral treatment agent?\nbs-collection_device,Optional,\"Instrument or container used to collect sample, e.g., swab\"\nbs-collection_method,Optional,\"Process used to collect the sample, e.g., bronchoalveolar lavage (BAL)\"\nbs-date_of_prior_antiviral_treat,Optional,\"Date of the SARS-CoV-2 antiviral treatment, e.g., 2021-03-30\"\nbs-date_of_prior_sars_cov_2_infection,Optional,\"Date of the prior SARS-CoV-2 infection, e.g., 2021-03-30\"\nbs-date_of_sars_cov_2_vaccination,Optional,\"Date of the 1st dose of the SARS-CoV-2 vaccine, e.g., 2021-03-30\"\nbs-exposure_event,Optional,\"Event leading to exposure, e.g., mass gathering\"\nbs-geo_loc_exposure,Optional,\"The country where the host was likely exposed to the causative agent of the illness. This location pertains to the country the host was believed to be exposed, and may not be the same as the host's country of residence, e.g., Canada\"\nbs-gisaid_accession,Optional,\"The GISAID accession assigned to the sequence. GISAID Accession Numbers are used as unique and permanent identifiers for each virus beginning with the letters EPI and followed by numbers, to identify viruses and/or segments; https://www.gisaid.org/; e.g., EPI_ISL_1091361\"\nbs-gisaid_virus_name,Optional,\"The full virus name submitted to GISAID (https://www.gisaid.org/), e.g., hCoV-19/Belgium/rega-3187/2021\"\nbs-host_age,Optional,Age of host at the time of sampling\nbs-host_anatomical_material,Optional,\"Host anatomical material or substance produced by the body where the sample was obtained, e.g., stool, mucus, saliva\"\nbs-host_anatomical_part,Optional,\"Anatomical part of the host organism (e.g. tissue) that was sampled, e.g., nasopharynx\"\nbs-host_body_product,Optional,\"substance produced by the host, e.g. stool, mucus, where the sample was obtained from\"\nbs-host_disease_outcome,Optional,\"Final outcome of disease, e.g., death, chronic disease, recovery\"\nbs-host_health_state,Optional,Information regarding health state of the individual sampled at the time of sampling\nbs-host_recent_travel_loc,Optional,\"The name of the country that was the destination of most recent travel. Specify the countries (and more granular locations if known) travelled, e.g., United Kingdom. Can include multiple travels; separate multiple travel events with a semicolon.\"\nbs-host_recent_travel_return_date,Optional,\"The date of a person's most recent return to some residence from a journey originating at that residence, e.g., 2021-03-30\"\nbs-host_sex,Optional,Gender or physical sex of the host\nbs-host_specimen_voucher,Optional,\"Identifier for the physical specimen. Include a URI (Uniform Resource Identifier) in the form of a URL providing a direct link to the physical host specimen. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical host voucher specimen. If a URI is not available, a museum-provided globally unique identifier (GUID) can be used. URI example: http://portal.vertnet.org/o/fmnh/mammals?id=33e55cfe-330b-40d9-aaae-8d042cba7542; INSDC triplet example: UAM:Mamm:52179\"\nbs-host_subject_id,Optional,\"a unique identifier by which each subject can be referred to, de-identified, e.g. #131\"\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-passage_method,Optional,\"Description of how the organism was passaged. Provide a short description, e.g., AVL buffer+30%EtOH lysate received from Respiratory Lab. P3 passage in Vero-1 via bioreactor large-scale batch passage. P3 batch derived from the SP-2/reference lab strain. If not passaged, put \"\"\"\"not applicable\"\"\"\".\"\nbs-passage_number,Optional,\"The number of known passages, e.g., 3. If not passaged, put \"\"\"\"not applicable\"\"\"\".\"\nbs-prior_sars_cov_2_antiviral_treat,Optional,Has the host received SARS-CoV-2 antiviral treatment?\nbs-prior_sars_cov_2_infection,Optional,Did the host have a prior SARS-CoV-2 infection?\nbs-prior_sars_cov_2_vaccination,Optional,Has the host received a SARS-CoV-2 vaccination?\nbs-purpose_of_sampling,Optional,\"the reason that the sample was collected, e.g., active surveillance in response to an outbreak, active surveillance not initiated by an outbreak, clinical trial, cluster investigation, environmental assessment, farm sample, field trial, for cause, industry internal investigation, market sample, passive surveillance, population based studies, research, research and development\"\nbs-purpose_of_sequencing,Optional,\"The reason the sample was sequenced, e.g., baseline surveillance (random sampling)\"\nbs-sars_cov_2_diag_gene_name_1,Optional,The name of the gene used in the first diagnostic SARS-CoV-2 RT-PCR test.\nbs-sars_cov_2_diag_gene_name_2,Optional,The name of the gene used in the second diagnostic SARS-CoV-2 RT-PCR test.\nbs-sars_cov_2_diag_pcr_ct_value_1,Optional,\"The cycle threshold (CT) value result from the first diagnostic SARS-CoV-2 RT-PCR test, e.g., 21\"\nbs-sars_cov_2_diag_pcr_ct_value_2,Optional,\"The cycle threshold (CT) value result from the second diagnostic SARS-CoV-2 RT-PCR test, e.g., 36\"\nbs-sequenced_by,Optional,\"The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention\"\nbs-vaccine_received,Optional,\"Which vaccine did the host receive, e.g., Pfizer-BioNTech COVID-19 vaccine\"\nbs-virus_isolate_of_prior_infection,Optional,\"Specific isolate of SARS-CoV-2 in prior infection (if known), e.g., SARS-CoV-2/human/USA/CA-CDPH-001/2020\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.SARS-CoV-2.wwsurv.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-ww_population,Required,\"Number of persons contributing wastewater to this sample collection site; if unknown, estimate to the nearest order of magnitude, e.g., 10000. If no estimate is available, input NA.\"\nbs-ww_sample_duration,Required,\"Duration of composite sample collected, in units of hours, e.g., 24. Specify integer values. If the sample is not a composite sample, use 0.\"\nbs-ww_sample_matrix,Required,The wastewater matrix that was sampled\nbs-ww_sample_type,Required,Type of wastewater sample collected\nbs-ww_surv_target_1,Required,\"Taxonomic name of the surveillance target. For the COVID-19 response, use 'SARS-CoV-2'.\"\nbs-ww_surv_target_1_known_present,Required,\"Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection.\"\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-purpose_of_ww_sampling,Optional,The reason the sample was collected\nbs-purpose_of_ww_sequencing,Optional,\"The reason the sample was sequenced, e.g., identification of mutations within a specific region, presence of clinically known mutations, or diversity of mutations across entire genome\"\nbs-sequenced_by,Optional,\"The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention\"\nbs-ww_endog_control_1,Optional,\"The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., pepper mild mottle virus\"\nbs-ww_endog_control_1_conc,Optional,\"The concentration of the endogenous control specified in 'ww_endog_control_1' on a per wastewater unit basis, e.g., 700000000\"\nbs-ww_endog_control_1_protocol,Optional,\"The protocol used to quantify 'ww_endog_control_1'. Specify a reference, website, or brief description.\"\nbs-ww_endog_control_1_units,Optional,\"The units of the value specified in 'ww_endog_control_1_conc', e.g., copies/L wastewater\"\nbs-ww_endog_control_2,Optional,\"The name of an organism, gene, or compound used as an endogenous wastewater control, e.g., crassphage\"\nbs-ww_endog_control_2_conc,Optional,\"The concentration of the endogenous control specified in 'ww_endog_control_2' on a per wastewater unit basis, e.g., 140000000\"\nbs-ww_endog_control_2_protocol,Optional,\"The protocol used to quantify 'ww_endog_control_2'. Specify a reference, website, or brief description.\"\nbs-ww_endog_control_2_units,Optional,\"The units of the value specified in 'ww_endog_control_2_conc', e.g., copies/L wastewater\"\nbs-ww_flow,Optional,\"Daily volumetric flow through collection site, in units of liters per day, e.g., 110000000.\"\nbs-ww_industrial_effluent_percent,Optional,\"Percentage of industrial effluents received by wastewater treatment plant, e.g., 10\"\nbs-ww_ph,Optional,\"pH measurement of the sample, or liquid portion of sample, or aqueous phase of the fluid, e.g., 7.2\"\nbs-ww_population_source,Optional,\"Source of value specified in 'ww_population', e.g., wastewater utility billing records, population of jurisdiction encompassing the wastewater service area, census blocks clipped to wastewater service area polygon\"\nbs-ww_pre_treatment,Optional,\"Describe any process of pre-treatment that removes materials that can be easily collected from the raw wastewater, e.g., flow equilibration basin promotes settling of some solids\"\nbs-ww_primary_sludge_retention_time,Optional,\"The time primary sludge remains in tank, in hours, e.g., 4.\"\nbs-ww_processing_protocol,Optional,\"The protocol used to process the wastewater sample. Processing includes laboratory procedures prior to and including nucleic acid purification (e.g., pasteurization, concentration, extraction, etc). Specify a reference, website, or brief description.\"\nbs-ww_sample_salinity,Optional,\"Salinity is the total concentration of all dissolved salts in a liquid or solid (in the form of an extract obtained by centrifugation) sample or derived from the conductivity measurement (practical salinity) in milligrams per liter, e.g., 100.\"\nbs-ww_sample_site,Optional,The type of site where the wastewater sample was collected\nbs-ww_surv_jurisdiction,Optional,\"A jurisdiction identifer that can be used to support linking the sample to a public health surveillance system, e.g., va\"\nbs-ww_surv_system_sample_id,Optional,\"The sample ID used for submission to a public health surveillance system (e.g., CDC's National Wastewater Surveillance System), e.g., s123456\"\nbs-ww_surv_target_1_conc,Optional,\"The concentration of the wastewater surveillance target specified in 'ww_surv_target_1' on a per wastewater unit basis, e.g., 200000\"\nbs-ww_surv_target_1_conc_unit,Optional,\"The units of the value specified in 'ww_surv_target_1_conc', e.g., copies/L wastewater\"\nbs-ww_surv_target_1_extract,Optional,\"Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 100000\"\nbs-ww_surv_target_1_extract_unit,Optional,\"The units of the value specified in 'ww_surv_target_1_extract', e.g., copies/microliter extract\"\nbs-ww_surv_target_1_gene,Optional,\"The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_1', e.g., N gene\"\nbs-ww_surv_target_1_protocol,Optional,\"The protocol used to quantify 'ww_surv_target_1'. Specify a reference, website, or brief description.\"\nbs-ww_surv_target_2,Optional,\"Taxonomic name of the surveillance target, eg, Norovirus\"\nbs-ww_surv_target_2_conc,Optional,\"The concentration of the wastewater surveillance target specified in 'ww_surv_target_2' on a per wastewater unit basis, e.g., 24000\"\nbs-ww_surv_target_2_conc_unit,Optional,\"The units of the value specified in 'ww_surv_target_2_conc', e.g., copies/L wastewater\"\nbs-ww_surv_target_2_extract,Optional,\"Measured amount of surveillance target in the nucleic acid extract that was sequenced; on a per extract unit basis, rather than on a per wastewater sample unit basis, e.g., 12000\"\nbs-ww_surv_target_2_extract_unit,Optional,\"The units of the value specified in 'ww_surv_target_2_extract', e.g., copies/microliter extract\"\nbs-ww_surv_target_2_gene,Optional,\"The name of the gene quantified for the the surveillance target specified in 'ww_surv_target_2', e.g., ORF1-ORF2 junction\"\nbs-ww_surv_target_2_known_present,Optional,\"Is genetic material of the surveillance target(s) known to the submitter to be present in this wastewater sample? Presence defined as microbiological evidence of the target organism in the wastewater sample, such as genetic- or culture-based detection.\"\nbs-ww_surv_target_2_protocol,Optional,\"The protocol used to quantify 'ww_surv_target_2'. Specify a reference, website, or brief description.\"\nbs-ww_temperature,Optional,\"Temperature of the wastewater sample at the time of sampling in Celsius, e.g., 25.\"\nbs-ww_total_suspended_solids,Optional,\"Total concentration of solids in raw wastewater influent sample including a wide variety of material, such as silt, decaying plant and animal matter in milligrams per liter, e.g., 500.\"\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.biosample.Virus.1.0_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.\nbs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.\nbs-sample_description,Optional,Optional description for sample.\nbs-isolate,Required,identification or description of the specific individual from which this sample was obtained\nbs-host,\"At least one field required. Group: At least one required: Group \"\"Host\",\"At least one required: Group \"\"Host\"\". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, \"\"Homo sapiens\"\".\"\nbs-lab_host,\"At least one field required. Group: At least one required: Group \"\"Host\",\"At least one required: Group \"\"Host\"\". Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained, e.g., Escherichia coli DH5a, or Homo sapiens HeLa cells\"\nbs-geo_loc_name,Required,\"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg \"\"Canada: Vancouver\"\" or \"\"Germany: halfway down Zugspitze, Alps\"\"\"\nbs-isolation_source,Required,\"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived.\"\nbs-altitude,Optional,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.\nbs-biomaterial_provider,Optional,\"name and address of the lab or PI, or a culture collection identifier\"\nbs-collected_by,Optional,Name of persons or institute who collected the sample\nbs-culture_collection,Optional,\"Name of source institute and unique culture identifier. See the description for the proper format and list of allowed institutes, http://www.insdc.org/controlled-vocabulary-culturecollection-qualifier\"\nbs-depth,Optional,\"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples.\"\nbs-disease,Optional,\"list of diseases diagnosed; can include multiple diagnoses. the value of the field depends on host; for humans the terms should be chosen from DO (Disease Ontology), free text for non-human. For DO terms, please see http://gemina.svn.sourceforge.net/viewvc/gemina/trunk/Gemina/ontologies/gemina_symptom.obo?view=log\"\nbs-env_broad_scale,Optional,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]\nbs-genotype,Optional,observed genotype\nbs-host_tissue_sampled,Optional,\"name of body site where the sample was obtained from, such as a specific organ or tissue, e.g., tongue, lung. For foundational model of anatomy ontology (fma) (v 4.11.0) or Uber-anatomy ontology (UBERON) (v releases/2014-06-15) terms, please see http://purl.bioontology.org/ontology/FMA or http://purl.bioontology.org/ontology/UBERON\"\nbs-identified_by,Optional,name of the taxonomist who identified the specimen\nbs-lat_lon,Optional,\"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format \"\"d[d.dddd] N|S d[dd.dddd] W|E\"\", eg, 38.98 N 77.11 W\"\nbs-passage_history,Optional,Number of passages and passage method\nbs-samp_size,Optional,\"Amount or size of sample (volume, mass or area) that was collected\"\nbs-serotype,Optional,\"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serogroup. e.g. serotype=\"\"H1N1\"\" in Influenza A virus CY098518.\"\nbs-specimen_voucher,Optional,\"Identifier for the physical specimen. Use format: \"\"[:[:]]\"\", eg, \"\"UAM:Mamm:52179\"\". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier.\"\nbs-strain,Optional,microbial or eukaryotic strain name\nbs-temp,Optional,temperature of the sample at time of sampling\nbs-title,Optional,\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-BS\"\" will be used instead.\"\nbs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.genbank.genbank.cmt.schema_template.csv", "content": "column_name,required_column,description\n((?:StructuredComment)(?:Prefix|Suffix)),Required,\"Structured comment keyword. For FLU use \"\"FluData\"\", HIV use \"\"HIV-DataBaseData\"\", and for COV and other organisms use \"\"Assembly-Data\"\".\"\n", "type": "text"}, {"name": "templates/config.genbank.genbank.flu.src.schema_template.csv", "content": "column_name,required_column,description\nsrc-Altitude,Optional,Altitude in metres above or below sea level of where the sample was collected.\nsrc-Authority,Optional,The author or authors of the organism name from which sequence was obtained.\nsrc-Bio_material,Optional,\"An identifier for the biological material from which the nucleotide sequence was obtained, with optional institution code and collection code for the place where it is currently stored. This should be provided using the following format 'institution-code:collection-code:material_id'. material_id is mandatory, institution-code and collection-code are optional; institution-code is mandatory when collection-code is present. This qualifier should be used to annotate the identifiers of material in biological collections which include zoos and aquaria, stock centers, seed banks, germplasm repositories and DNA banks.\"\nsrc-Biotype,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by some specific biological property (often geographical, ecological, or physiological). Same as biotype.\"\nsrc-Biovar,Optional,See biotype\nsrc-Breed,Optional,The named breed from which sequence was obtained (usually applied to domesticated mammals).\nsrc-Cell_line,Optional,Cell line from which sequence was obtained.\nsrc-Cell_type,Optional,Type of cell from which sequence was obtained.\nsrc-Chemovar,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by its biochemical properties.\"\nsrc-Clone,Optional,Name of clone from which sequence was obtained.\nsrc-Collected_by,Optional,Name of person who collected the sample.\nsrc-Country,Optional,\"The country where the sequence's organism was located. May also be an ocean or major sea. Additional region or locality information must be after the country name and separated by a ':'. For example: USA: Riverview Park, Ripkentown, MD\"\nsrc-Cultivar,Optional,Cultivated variety of plant from which sequence was obtained.\nsrc-Culture_collection,Optional,\"Institution code and identifier for the culture from which the nucleotide sequence was obtained, with optional collection code. This should be provided using the following format 'institution-code:collection-code:culture-id'. culture-id and institution-code are mandatory. This qualifier should be used to annotate live microbial and viral cultures, and cell lines that have been deposited in curated culture collections.\"\nsrc-Dev_stage,Optional,Developmental stage of organism.\nsrc-Ecotype,Optional,The named ecotype (population adapted to a local habitat) from which sequence was obtained (customarily applied to populations of Arabidopsis thaliana).\nsrc-Forma,Optional,The forma (lowest taxonomic unit governed by the nomenclatural codes) of organism from which sequence was obtained. This term is usually applied to plants and fungi.\nsrc-Forma_specialis,Optional,The physiologically distinct form from which sequence was obtained (usually restricted to certain parasitic fungi).\nsrc-Fwd_primer_name,Optional,name of forward PCR primer\nsrc-Fwd_primer_seq,Optional,nucleotide sequence of forward PCR primer\nsrc-Genotype,Optional,Genotype of the organism.\nsrc-Haplogroup,Optional,Name for a group of similar haplotypes that share some sequence variation\nsrc-Haplotype,Optional,Haplotype of the organism.\nsrc-Host,Optional,\"When the sequence submission is from an organism that exists in a symbiotic, parasitic, or other special relationship with some second organism, the 'host' modifier can be used to identify the name of the host species.\"\nsrc-Identified_by,Optional,name of the person or persons who identified by taxonomic name the organism from which the sequence was obtained\nsrc-Isolate,Optional,Identification or description of the specific individual from which this sequence was obtained.\nsrc-Isolation-source,Optional,Describes the local geographical source of the organism from which the sequence was obtained.\nsrc-Lab_host,Optional,Laboratory host used to propagate the organism from which the sequence was obtained.\nsrc-Lat_Lon,Optional,\"Latitude and longitude, in decimal degrees, of where the sample was collected.\"\nsrc-Note,Optional,Any additional information that you wish to provide about the sequence.\nsrc-Pathovar,Optional,\"Variety of a species (usually a fungus, bacteria or virus) characterized by the biological target of the pathogen. Examples include Pseudomonas syringae pathovar tomato and Pseudomonas syringae pathovar tabaci.\"\nsrc-Pop_variant,Optional,name of the population variant from which the sequence was obtained\nsrc-Rev_primer_name,Optional,name of reverse PCR primer\nsrc-Rev_primer_seq,Optional,nucleotide sequence of reverse PCR primer\nsrc-Segment,Optional,name of viral or phage segment sequenced\nsrc-Serogroup,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by its antigenic properties. Same as serogroup and serovar.\"\nsrc-Serotype,Optional,See Serogroup\nsrc-Serovar,Optional,See Serogroup\nsrc-Sex,Optional,Sex of the organism from which the sequence was obtained.\nsrc-Specimen_voucher,Optional,\"An identifier of the individual or collection of the source organism and the place where it is currently stored, usually an institution. This should be provided using the following format 'institution-code:collection-code:specimen-id'. specimen-id is mandatory, collection-code is optional; institution-code is mandatory when collection-code is provided. Examples: 99-SRNP UAM:Mamm:52179 personal collection:Joe Smith:99-SRNP AMCC:101706\"\nsrc-Strain,Required,Strain of organism from which sequence was obtained.\nsrc-Sub_species,Optional,Subspecies of organism from which sequence was obtained.\nsrc-Subclone,Optional,Name of subclone from which sequence was obtained.\nsrc-Subtype,Optional,Subtype of organism from which sequence was obtained.\nsrc-Substrain,Optional,Sub-strain of organism from which sequence was obtained.\nsrc-Tissue_lib,Optional,Tissue library from which the sequence was obtained.\nsrc-Tissue_type,Optional,Type of tissue from which sequence was obtained.\nsrc-Type,Optional,Type of organism from which sequence was obtained.\nsrc-Variety,Optional,Variety of organism from which sequence was obtained.\n", "type": "text"}, {"name": "templates/config.genbank.genbank.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.\ngb-sample_name,Required,Identifier name used for GenBank. Max length is 50 characters.\ngb-fasta_definition_line_modifiers,Required,\"NCBI fasta definition line modifiers can be added here. As many modifiers as you like can be added, but each must bounded by a set of brackets. Some of the available keywords are listed at \"\"https://www.ncbi.nlm.nih.gov/genbank/mods_fastadefline/\"\".\"\ngb-title,Optional,\"Optional internal field for how the GenBank submission should be named when viewed from the NCBI submission portal, . If not provided, when performing submissions <--submission_name> with the suffix \"\"-GB\"\" will be used instead.\"\nsra-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}, {"name": "templates/config.genbank.genbank.src.schema_template.csv", "content": "column_name,required_column,description\nsrc-Altitude,Optional,Altitude in metres above or below sea level of where the sample was collected.\nsrc-Authority,Optional,The author or authors of the organism name from which sequence was obtained.\nsrc-Bio_material,Optional,\"An identifier for the biological material from which the nucleotide sequence was obtained, with optional institution code and collection code for the place where it is currently stored. This should be provided using the following format 'institution-code:collection-code:material_id'. material_id is mandatory, institution-code and collection-code are optional; institution-code is mandatory when collection-code is present. This qualifier should be used to annotate the identifiers of material in biological collections which include zoos and aquaria, stock centers, seed banks, germplasm repositories and DNA banks.\"\nsrc-Biotype,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by some specific biological property (often geographical, ecological, or physiological). Same as biotype.\"\nsrc-Biovar,Optional,See biotype\nsrc-Breed,Optional,The named breed from which sequence was obtained (usually applied to domesticated mammals).\nsrc-Cell_line,Optional,Cell line from which sequence was obtained.\nsrc-Cell_type,Optional,Type of cell from which sequence was obtained.\nsrc-Chemovar,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by its biochemical properties.\"\nsrc-Clone,Optional,Name of clone from which sequence was obtained.\nsrc-Collected_by,Optional,Name of person who collected the sample.\nsrc-Country,Optional,\"The country where the sequence's organism was located. May also be an ocean or major sea. Additional region or locality information must be after the country name and separated by a ':'. For example: USA: Riverview Park, Ripkentown, MD\"\nsrc-Cultivar,Optional,Cultivated variety of plant from which sequence was obtained.\nsrc-Culture_collection,Optional,\"Institution code and identifier for the culture from which the nucleotide sequence was obtained, with optional collection code. This should be provided using the following format 'institution-code:collection-code:culture-id'. culture-id and institution-code are mandatory. This qualifier should be used to annotate live microbial and viral cultures, and cell lines that have been deposited in curated culture collections.\"\nsrc-Dev_stage,Optional,Developmental stage of organism.\nsrc-Ecotype,Optional,The named ecotype (population adapted to a local habitat) from which sequence was obtained (customarily applied to populations of Arabidopsis thaliana).\nsrc-Forma,Optional,The forma (lowest taxonomic unit governed by the nomenclatural codes) of organism from which sequence was obtained. This term is usually applied to plants and fungi.\nsrc-Forma_specialis,Optional,The physiologically distinct form from which sequence was obtained (usually restricted to certain parasitic fungi).\nsrc-Fwd_primer_name,Optional,name of forward PCR primer\nsrc-Fwd_primer_seq,Optional,nucleotide sequence of forward PCR primer\nsrc-Genotype,Optional,Genotype of the organism.\nsrc-Haplogroup,Optional,Name for a group of similar haplotypes that share some sequence variation\nsrc-Haplotype,Optional,Haplotype of the organism.\nsrc-Host,Optional,\"When the sequence submission is from an organism that exists in a symbiotic, parasitic, or other special relationship with some second organism, the 'host' modifier can be used to identify the name of the host species.\"\nsrc-Identified_by,Optional,name of the person or persons who identified by taxonomic name the organism from which the sequence was obtained\nsrc-Isolate,Optional,Identification or description of the specific individual from which this sequence was obtained.\nsrc-Isolation-source,Optional,Describes the local geographical source of the organism from which the sequence was obtained.\nsrc-Lab_host,Optional,Laboratory host used to propagate the organism from which the sequence was obtained.\nsrc-Lat_Lon,Optional,\"Latitude and longitude, in decimal degrees, of where the sample was collected.\"\nsrc-Note,Optional,Any additional information that you wish to provide about the sequence.\nsrc-Pathovar,Optional,\"Variety of a species (usually a fungus, bacteria or virus) characterized by the biological target of the pathogen. Examples include Pseudomonas syringae pathovar tomato and Pseudomonas syringae pathovar tabaci.\"\nsrc-Pop_variant,Optional,name of the population variant from which the sequence was obtained\nsrc-Rev_primer_name,Optional,name of reverse PCR primer\nsrc-Rev_primer_seq,Optional,nucleotide sequence of reverse PCR primer\nsrc-Segment,Optional,name of viral or phage segment sequenced\nsrc-Serogroup,Optional,\"Variety of a species (usually a fungus, bacteria, or virus) characterized by its antigenic properties. Same as serogroup and serovar.\"\nsrc-Serotype,Optional,See Serogroup\nsrc-Serovar,Optional,See Serogroup\nsrc-Sex,Optional,Sex of the organism from which the sequence was obtained.\nsrc-Specimen_voucher,Optional,\"An identifier of the individual or collection of the source organism and the place where it is currently stored, usually an institution. This should be provided using the following format 'institution-code:collection-code:specimen-id'. specimen-id is mandatory, collection-code is optional; institution-code is mandatory when collection-code is provided. Examples: 99-SRNP UAM:Mamm:52179 personal collection:Joe Smith:99-SRNP AMCC:101706\"\nsrc-Strain,Optional,Strain of organism from which sequence was obtained.\nsrc-Sub_species,Optional,Subspecies of organism from which sequence was obtained.\nsrc-Subclone,Optional,Name of subclone from which sequence was obtained.\nsrc-Subtype,Optional,Subtype of organism from which sequence was obtained.\nsrc-Substrain,Optional,Sub-strain of organism from which sequence was obtained.\nsrc-Tissue_lib,Optional,Tissue library from which the sequence was obtained.\nsrc-Tissue_type,Optional,Type of tissue from which sequence was obtained.\nsrc-Type,Optional,Type of organism from which sequence was obtained.\nsrc-Variety,Optional,Variety of organism from which sequence was obtained.\n", "type": "text"}, {"name": "templates/config.gisaid.gisaid.ARBO.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.\ngs-sample_name,Required,\"Identifier name used for GISAID. Max length is 50 characters. This field is the same as \"\"arbo_sequence_name\"\" in GISAID's metadata template.\"\ngs-arbo_type,Required,\"For hCoV-19, this will always be \"\"Chikungunya virus\"\".\"\ngs-arbo_passage,Required,\"\"\"Original\"\" if the sample was sequenced directly from swabs, otherwise add the name of the cell line (e.g., \"\"Vero\"\") used to culture the specimen.\"\ngs-arbo_location,Required,\"Format as \"\"Continent / Country / Region / Sub-region\"\".\"\ngs-arbo_add_location,Optional,\"Additional location information (e.g. Cruise Ship, Convention, Live animal market).\"\ngs-arbo_host,Required,\"Host species name. For Wastewater use \"\"Environment\"\".\"\ngs-arbo_add_host_info,Optional,Additional information regarding patient (e.g. Patient infected while interacting with animal).\ngs-arbo_sampling_strategy,Optional,\"Sampling strategy for sequence (e.g. Sentinel surveillance (ILI), Sentinel surveillance (ARI), Sentinel surveillance (SARI), Non-sentinel-surveillance (hospital), Non-sentinel-surveillance (GP network), Longitudinal sampling on same patient(s), S gene dropout).\"\ngs-arbo_gender,Required,\"Synonym for \"\"Biological sex\"\". Should be \"\"Female\"\", \"\"Male\"\", or \"\"Unknown\"\".\"\ngs-arbo_patient_age,Required,\"Age in years of the person from whom the specimen was collected. May take format other than numeric years, for example, \"\"0.5\"\" (i.e., 6 months), \"\"5 days\"\", \"\"7 months\"\". If units are not given, they are assumed in years. If missing, use \"\"Unknown\"\".\"\ngs-arbo_patient_status,Required,\"E.g., \"\"Hospitalized\"\", \"\"Released\"\", \"\"Live\"\", \"\"Deceased\"\", \"\"Unknown\"\".\"\ngs-arbo_disease_manifestation,Optional,\"Mild, Severe (e.g Guillain-Barr\u00e9 syndrome (GBS)/ encephalitis/ microcephaly), Fatal.\"\ngs-arbo_clinical_symptoms,Optional,\"e.g. fever, rash ,nausea, vomiting, retro-orbital pain, muscle pain.\"\ngs-arbo_specimen,Optional,\"Specimen source. For wastewater it must be \"\"Wastewater surveillance\"\".\"\ngs-arbo_outbreak,Optional,\"Outbreak information (Date, Location e.g. type of gathering, Family cluster, etc.).\"\ngs-arbo_last_vaccinated,Optional,Provide details if applicable.\ngs-arbo_treatment,Optional,\"Provide details if applicable (e.g. Drug name, dosage).\"\ngs-arbo_seq_technology,Required,\"Add the sequencer brand and model (e.g. Illumina MiSeq, Sanger, Nanopore MinION).\"\ngs-arbo_assembly_method,Optional,\"Genome assembly algorithm (e.g. CLC Genomics Workbench 12, Geneious 10.2.4, SPAdes/MEGAHIT v1.2.9, UGENE v. 33).\"\ngs-arbo_coverage,Optional,\"Average genome coverage (e.g. 50x, 100x, 1,000x).\"\ngs-arbo_publications,Optional,e.g. DOI: 10.1081/15588742.2015.1017687.\ngs-arbo_orig_lab,Required,Full name of laboratory from where sample originated.\ngs-arbo_orig_lab_addr,Required,Complete building address of laboratory from where sample originated.\ngs-arbo_provider_sample_id,Optional,ID used by originating lab.\ngs-arbo_subm_lab,Required,Full name of laboratory submitting this record to GISAID.\ngs-arbo_subm_lab_addr,Required,Complete building address of the submitting laboratory.\ngs-arbo_subm_sample_id,Optional,ID used by submitting lab.\ngs-arbo_consortium,Optional,Sequencing consortium the submitting lab is affiliated with.\ngs-arbo_comment,Required,Leave blank.\ngs-comment_type,Required,Leave blank.\n", "type": "text"}, {"name": "templates/config.gisaid.gisaid.COV.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.\ngs-sample_name,Required,\"Identifier name used for GISAID. Max length is 50 characters. This field is the same as \"\"covv_sequence_name\"\" in GISAID's metadata template.\"\ngs-covv_type,Required,\"For hCoV-19, this will always be \"\"betacoronavirus\"\".\"\ngs-covv_passage,Required,\"\"\"Original\"\" if the sample was sequenced directly from swabs, otherwise add the name of the cell line (e.g., \"\"Vero\"\") used to culture the specimen.\"\ngs-covv_location,Required,\"Format as \"\"Continent / Country / Region / Sub-region\"\".\"\ngs-covv_add_location,Optional,\"Additional location information (e.g. Cruise Ship, Convention, Live animal market).\"\ngs-covv_host,Required,\"Host species name. For Wastewater use \"\"Environment\"\".\"\ngs-covv_add_host_info,Optional,Additional information regarding patient (e.g. Patient infected while interacting with animal).\ngs-covv_sampling_strategy,Optional,\"Sampling strategy for sequence (e.g. Sentinel surveillance (ILI), Sentinel surveillance (ARI), Sentinel surveillance (SARI), Non-sentinel-surveillance (hospital), Non-sentinel-surveillance (GP network), Longitudinal sampling on same patient(s), S gene dropout).\"\ngs-covv_gender,Required,\"Synonym for \"\"Biological sex\"\". Should be \"\"Female\"\", \"\"Male\"\", or \"\"Unknown\"\".\"\ngs-covv_patient_age,Required,\"Age in years of the person from whom the specimen was collected. May take format other than numeric years, for example, \"\"0.5\"\" (i.e., 6 months), \"\"5 days\"\", \"\"7 months\"\". If units are not given, they are assumed in years. If missing, use \"\"Unknown\"\".\"\ngs-covv_patient_status,Required,\"E.g., \"\"Hospitalized\"\", \"\"Released\"\", \"\"Live\"\", \"\"Deceased\"\", \"\"Unknown\"\".\"\ngs-covv_specimen,Optional,\"Specimen source. For wastewater it must be \"\"Wastewater surveillance\"\".\"\ngs-covv_outbreak,Optional,\"Outbreak information (Date, Location e.g. type of gathering, Family cluster, etc.).\"\ngs-covv_last_vaccinated,Optional,Provide details if applicable.\ngs-covv_treatment,Optional,\"Provide details if applicable (e.g. Drug name, dosage).\"\ngs-covv_seq_technology,Required,\"Add the sequencer brand and model (e.g. Illumina MiSeq, Sanger, Nanopore MinION).\"\ngs-covv_assembly_method,Optional,\"Genome assembly algorithm (e.g. CLC Genomics Workbench 12, Geneious 10.2.4, SPAdes/MEGAHIT v1.2.9, UGENE v. 33).\"\ngs-covv_coverage,Optional,\"Average genome coverage (e.g. 50x, 100x, 1,000x).\"\ngs-covv_orig_lab,Required,Full name of laboratory from where sample originated.\ngs-covv_orig_lab_addr,Required,Complete building address of laboratory from where sample originated.\ngs-covv_provider_sample_id,Optional,ID used by originating lab.\ngs-covv_subm_lab,Required,Full name of laboratory submitting this record to GISAID.\ngs-covv_subm_lab_addr,Required,Complete building address of the submitting laboratory.\ngs-covv_subm_sample_id,Optional,ID used by submitting lab.\ngs-covv_consortium,Optional,Sequencing consortium the submitting lab is affiliated with.\ngs-covv_comment,Required,Leave blank.\ngs-comment_type,Required,Leave blank.\n", "type": "text"}, {"name": "templates/config.gisaid.gisaid.FLU.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.\ngs-sample_name,Required,Identifier name for sample segment used for GISAID. Max length is 50 characters.\ngs-Isolate_Name,Required,Identifier name for sample used for GISAID. Max length is 50 characters.\ngs-segment,Required,Segment name for sequence.\ngs-Subtype,Required,Influenza subtype (e.g. H5N1).\ngs-Lineage,Optional,Lineage information.\ngs-Passage_History,Required,\"\"\"Original\"\" if the sample was sequenced directly from swabs, otherwise add the name of the cell line (e.g., \"\"Vero\"\") used to culture the specimen.\"\ngs-Location,Required,Country.\ngs-province,Required,Province/State.\ngs-sub_province,Optional,\"Local region name, county, territory, etc.\"\ngs-Location_Additional_info,Optional,\"Additional location information (e.g. Cruise Ship, Convention, Live animal market).\"\ngs-Host,Required,\"Host species name. For Wastewater use \"\"Environment\"\".\"\ngs-Host_Additional_info,Optional,Additional information regarding patient (e.g. Patient infected while interacting with animal).\ngs-Submitting_Sample_Id,Optional,ID used by submitting lab.\ngs-Originating_Lab_Id,Optional,ID used for originating lab.\ngs-Originating_Sample_Id,Optional,ID used by originating lab.\ngs-Antigen_Character,Optional,\ngs-Adamantanes_Resistance_geno,Optional,\ngs-Oseltamivir_Resistance_geno,Optional,\ngs-Zanamivir_Resistance_geno,Optional,\ngs-Peramivir_Resistance_geno,Optional,\ngs-Other_Resistance_geno,Optional,\ngs-Host_Gender,Required,\"Synonym for \"\"Biological sex\"\". Should be \"\"Female\"\", \"\"Male\"\", or \"\"Unknown\"\".\"\ngs-Host_Age,Required,Numeric age for host.\ngs-Host_Age_Unit,Required,\"Unit of time for host age (e.g. Year is \"\"Y\"\", Month is \"\"M\"\").\"\ngs-Health_Status,Required,\"E.g., \"\"Hospitalized\"\", \"\"Released\"\", \"\"Live\"\", \"\"Deceased\"\", \"\"Unknown\"\".\"\ngs-Note,Optional,Sequence note.\ngs-PMID,Optional,PubMed ID\n", "type": "text"}, {"name": "templates/config.gisaid.gisaid.POX.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.\ngs-sample_name,Required,\"Identifier name used for GISAID. Max length is 50 characters. This field is the same as \"\"pox_sequence_name\"\" in GISAID's metadata template.\"\ngs-pox_passage,Required,\"\"\"Original\"\" if the sample was sequenced directly from swabs, otherwise add the name of the cell line (e.g., \"\"Vero\"\") used to culture the specimen.\"\ngs-pox_location,Required,\"Format as \"\"Continent / Country / Region / Sub-region\"\".\"\ngs-pox_add_location,Optional,\"Additional location information (e.g. Cruise Ship, Convention, Live animal market).\"\ngs-pox_host,Required,\"Host species name. For Wastewater use \"\"Environment\"\".\"\ngs-pox_add_host_info,Optional,Additional information regarding patient (e.g. Patient infected while interacting with animal).\ngs-pox_sampling_strategy,Optional,\"Sampling strategy for sequence (e.g. Sentinel surveillance (ILI), Sentinel surveillance (ARI), Sentinel surveillance (SARI), Non-sentinel-surveillance (hospital), Non-sentinel-surveillance (GP network), Longitudinal sampling on same patient(s), S gene dropout).\"\ngs-pox_gender,Required,\"Synonym for \"\"Biological sex\"\". Should be \"\"Female\"\", \"\"Male\"\", or \"\"Unknown\"\".\"\ngs-pox_patient_age,Required,\"Age in years of the person from whom the specimen was collected. May take format other than numeric years, for example, \"\"0.5\"\" (i.e., 6 months), \"\"5 days\"\", \"\"7 months\"\". If units are not given, they are assumed in years. If missing, use \"\"Unknown\"\".\"\ngs-pox_patient_status,Required,\"E.g., \"\"Hospitalized\"\", \"\"Released\"\", \"\"Live\"\", \"\"Deceased\"\", \"\"Unknown\"\".\"\ngs-pox_specimen,Optional,\"Specimen source. For wastewater it must be \"\"Wastewater surveillance\"\".\"\ngs-pox_outbreak,Optional,\"Outbreak information (Date, Location e.g. type of gathering, Family cluster, etc.).\"\ngs-pox_last_vaccinated,Optional,Provide details if applicable.\ngs-pox_treatment,Optional,\"Provide details if applicable (e.g. Drug name, dosage).\"\ngs-pox_seq_technology,Required,\"Add the sequencer brand and model (e.g. Illumina MiSeq, Sanger, Nanopore MinION).\"\ngs-pox_assembly_method,Optional,\"Genome assembly algorithm (e.g. CLC Genomics Workbench 12, Geneious 10.2.4, SPAdes/MEGAHIT v1.2.9, UGENE v. 33).\"\ngs-pox_coverage,Optional,\"Average genome coverage (e.g. 50x, 100x, 1,000x).\"\ngs-pox_orig_lab,Required,Full name of laboratory from where sample originated.\ngs-pox_orig_lab_addr,Required,Complete building address of laboratory from where sample originated.\ngs-pox_provider_sample_id,Optional,ID used by originating lab.\ngs-pox_subm_lab,Required,Full name of laboratory submitting this record to GISAID.\ngs-pox_subm_lab_addr,Required,Complete building address of the submitting laboratory.\ngs-pox_subm_sample_id,Optional,ID used by submitting lab.\ngs-pox_consortium,Optional,Sequencing consortium the submitting lab is affiliated with.\ngs-pox_comment,Required,Leave blank.\ngs-comment_type,Required,Leave blank.\n", "type": "text"}, {"name": "templates/config.seqsender.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank or GISAID.\norganism,Required,The most descriptive organism name for the samples.\nauthors,Required,\"Citing authors. List of Last, First Middle, suffix separated by a semicolon \"\";\"\" E.g.: \"\"Baker, Howard Henry, Jr.; Powell, Earl Alexander, III.;\"\"\"\ncollection_date,Required,\"Collection date for sequence. Must be in a valid format based on ISO 8601: \"\"YYYY-MM-DD\"\", \"\"YYYY-MM\"\", or \"\"YYYY\"\". Time is not supported.\"\nbioproject,Optional,NCBI BioProject ID.\n", "type": "text"}, {"name": "templates/config.seqsender.seqsender.schema_template.csv", "content": "column_name,required_column,description\nsequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank or GISAID.\norganism,Required,The most descriptive organism name for the samples.\nauthors,Required,\"Citing authors. List of Last, First Middle, suffix separated by a semicolon \"\";\"\" E.g.: \"\"Baker, Howard Henry, Jr.; Powell, Earl Alexander, III.;\"\"\"\ncollection_date,Required,\"Collection date for sample. Must be in a valid format based on ISO 8601: \"\"YYYY-MM-DD\"\", \"\"YYYY-MM\"\", or \"\"YYYY\"\". Time is not supported.\"\nbioproject,Optional,NCBI BioProject ID.\n", "type": "text"}, {"name": "templates/config.seqsender.submission.status.report.biosample.submission.status.report.schema_template.csv", "content": "column_name,required_column,description\nbs-sample_name,Required,Sample name used for BioSample submission.\nbiosample_status,Required,Current status of the BioSample submission.\nbiosample_accession,Required,Sample accession ID used to identify BioSample submission NCBI.\nbiosample_message,Required,\"If there is a message tied to the sample submitted to BioSample, it will appear here.\"\n", "type": "text"}, {"name": "templates/config.seqsender.submission.status.report.genbank.submission.status.report.schema_template.csv", "content": "column_name,required_column,description\ngb-sample_name,Required,Sample name used for GenBank submission.\ngenbank_status,Required,Current status of the GenBank submission.\ngenbank_accession,Required,Sample accession ID used to identify GenBank submission NCBI.\ngenbank_message,Required,\"If there is a message tied to the sequence submitted to GenBank, it will appear here.\"\n", "type": "text"}, {"name": "templates/config.seqsender.submission.status.report.gisaid.submission.status.report.schema_template.csv", "content": "column_name,required_column,description\ngs-sample_name,Required,Sample name used for GISAID submission.\ngs-segment_name,Optional,Segment name of sample used for GISAID submission.\ngisaid_accession_epi_isl_id,Required,Sample accession ID used to identify submission to GISAID.\ngisaid_accession_epi_id,Required,Sample accession ID used to identify submission in GISAID.\ngisaid_message,Required,\"If there is a message tied to the sequence submitted to GISAID, it will appear here.\"\n", "type": "text"}, {"name": "templates/config.seqsender.submission.status.report.sra.submission.status.report.schema_template.csv", "content": "column_name,required_column,description\nsra-sample_name,Required,Sample name used for SRA submission.\nsra_status,Required,Current status of the SRA submission.\nsra_accession,Required,Sample accession ID used to identify SRA submission NCBI.\nsra_message,Required,\"If there is a message tied to the sequence submitted to SRA, it will appear here.\"\n", "type": "text"}, {"name": "templates/config.sra.schema_template.csv", "content": "column_name,required_column,description\nsra-sample_name,Required,Identifier name used for SRA. Max length is 50 characters. Name must be unique from BioSample or Genbank submission.\nsra-file_location,Required,\"Location of raw reads files. Options: \"\"local\"\" or \"\"cloud\"\".\"\nsra-file_name,Required,\"Name of the raw read files. All file names must be unique and not contain any sensitive information. Files can be compressed using gzip or bzip2, and may be submitted in a tar archive but archiving and/or compressing your files is not required. Do not use zip! If there are multiple files, concatenate them with a commas (\"\",\"\"), e.g. \"\"sample1_R1.fastq.gz, sample1_R2.fastq.gz\"\". Store files in /seqsender/data/raw_reads/ or provide full html path to the raw read files.\"\nsra-library_name,Optional,Short unique identifier for sequencing library.\nsra-loader,Optional,\"SRA loader selection. If NCBI says your SRA files need to be uploaded with a certain loader, you can select it here.\"\nsra-library_strategy,Required,The sequencing technique intended for the library.\nsra-library_source,Required,The type of source material that is being sequenced.\nsra-library_selection,Required,The method used to select and/or enrich the material being sequenced.\nsra-library_layout,Required,\"Whether to expect SINGLE or PAIRED end reads. Options: \"\"single\"\" or \"\"paired\"\".\"\nsra-platform,Optional,Instrument platform.\nsra-instrument_model,Required,Instrument model used for sequencing.\nsra-design_description,Optional,Brief description of materials/methods used for creating sequencing library.\n", "type": "text"}, {"name": "templates/config.sra.sra.schema_template.csv", "content": "column_name,required_column,description\nsra-sample_name,Required,Identifier name used for SRA. Max length is 50 characters. Name must be unique from BioSample or Genbank submission.\nbs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters. Required if submitting to SRA.\nsra-file_location,Required,\"Location of raw reads files. Options: \"\"local\"\" or \"\"cloud\"\".\"\nsra-file_[1-9]\\d*,Required,\"Name of the raw read files. All file names must be unique and not contain any sensitive information. Files can be compressed using gzip or bzip2, and may be submitted in a tar archive but archiving and/or compressing your files is not required. Do not use zip! If there are multiple files, concatenate them with a commas (\"\",\"\"), e.g. \"\"sample1_R1.fastq.gz, sample1_R2.fastq.gz\"\". Store files in /seqsender/data/raw_reads/ or provide full html path to the raw read files.\"\nsra-library_name,Optional,Short unique identifier for sequencing library.\nsra-loader,Optional,\"SRA loader selection. If NCBI says your SRA files need to be uploaded with a certain loader, you can select it here.\"\nsra-library_strategy,Required,The sequencing technique intended for the library.\nsra-library_source,Required,The type of source material that is being sequenced.\nsra-library_selection,Required,The method used to select and/or enrich the material being sequenced.\nsra-library_layout,Required,\"Whether to expect SINGLE or PAIRED end reads. Options: \"\"single\"\" or \"\"paired\"\".\"\nsra-platform,Optional,Instrument platform.\nsra-instrument_model,Required,Instrument model used for sequencing.\nsra-design_description,Optional,Brief description of materials/methods used for creating sequencing library.\nsra-title,Optional,\"Optional internal field for how the SRA submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \"\"-SRA\"\" will be used instead.\"\nsra-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\n", "type": "text"}]
\ No newline at end of file
diff --git a/env.yaml b/env.yaml
index f128eb2..9178213 100644
--- a/env.yaml
+++ b/env.yaml
@@ -7,7 +7,7 @@ dependencies:
- _libgcc_mutex=0.1=conda_forge
- _openmp_mutex=4.5=2_gnu
- annotated-types=0.6.0=pyhd8ed1ab_0
- - biopython=1.83=py38h01eb140_0
+ - biopython=1.83
- brotli-python=1.1.0=py38h17151c0_1
- bzip2=1.0.8=hd590300_5
- ca-certificates=2024.2.2=hbcca054_0
diff --git a/file_handler.py b/file_handler.py
index bb3b1b4..517423f 100755
--- a/file_handler.py
+++ b/file_handler.py
@@ -99,8 +99,17 @@ def load_fasta_file(fasta_file: str) -> pd.DataFrame:
# Save submission xml
def save_xml(submission_xml: bytes, submission_dir: str) -> None:
# Save string as submission.xml
- with open(os.path.join(submission_dir, "submission.xml"), "wb") as file:
- file.write(submission_xml)
+ try:
+ with open(os.path.join(submission_dir, "submission.xml"), "wb") as file:
+ file.write(submission_xml)
+ except PermissionError as e:
+ print(f"Error: Permission error when trying to save 'submission.xml' to path: {submission_dir}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
+ except Exception as e:
+ print(f"Error: An unexpected error occurred when trying to save 'submission.xml' to path: {submission_dir}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
# Waiting for the xml file to write
while not os.path.exists(os.path.join(submission_dir, "submission.xml")):
time.sleep(10)
@@ -109,13 +118,34 @@ def save_xml(submission_xml: bytes, submission_dir: str) -> None:
def save_csv(df: pd.DataFrame, file_path: str, file_name: Optional[str] = None, sep: str = ",") -> None:
if file_name:
file_path = os.path.join(file_path, file_name)
- df.to_csv(file_path, header = True, index = False, sep = sep)
+ try:
+ df.to_csv(file_path, header = True, index = False, sep = sep)
+ except PermissionError as e:
+ print(f"Error: Permission error when trying to save '{file_name}' to path: {file_path}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
+ except Exception as e:
+ print(f"Error: An unexpected error occurred when trying to save '{file_name}' to path: {file_path}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
# Create fasta file based on database
def create_fasta(database: str, metadata: pd.DataFrame, submission_dir: str) -> None:
records = []
for index, row in metadata.iterrows():
column_name = SAMPLE_NAME_DATABASE_PREFIX[database] + "sample_name"
- records.append(SeqRecord(row["fasta_sequence_orig"], id = row[column_name], description = ""))
- with open(os.path.join(submission_dir, "sequence.fsa"), "w+") as f:
- SeqIO.write(records, f, "fasta")
+ if "GENBANK" in database and "gb-fasta_definition_line_modifiers" in metadata and pd.notnull(row["gb-fasta_definition_line_modifiers"]) and row["gb-fasta_definition_line_modifiers"].strip() != "":
+ records.append(SeqRecord(row["fasta_sequence_orig"], id =(row[column_name].strip() + " " + row["gb-fasta_definition_line_modifiers"].strip()), description = ""))
+ else:
+ records.append(SeqRecord(row["fasta_sequence_orig"], id = row[column_name], description = ""))
+ try:
+ with open(os.path.join(submission_dir, "sequence.fsa"), "w+") as f:
+ SeqIO.write(records, f, "fasta")
+ except PermissionError as e:
+ print(f"Error: Permission error when trying to save 'sequence.fsa' to path: {submission_dir}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
+ except Exception as e:
+ print(f"Error: An unexpected error occurred when trying to save 'sequence.fsa' to path: {submission_dir}", file=sys.stderr)
+ print(e, file=sys.stderr)
+ sys.exit(1)
diff --git a/genbank_handler.py b/genbank_handler.py
index 2443cee..7e11392 100644
--- a/genbank_handler.py
+++ b/genbank_handler.py
@@ -46,9 +46,15 @@ def create_submission_xml(organism: str, submission_name: str, config_dict: Dict
root = etree.Element("Submission")
description = etree.SubElement(root, "Description")
title = etree.SubElement(description, "Title")
- title.text = config_dict["Description"]["Title"]
+ if "gb-title" in metadata and pd.notnull(metadata["gb-title"].iloc[0]) and metadata["gb-title"].iloc[0].strip() != "":
+ title.text = metadata["gb-title"].iloc[0]
+ else:
+ title.text = submission_name + "-GB"
comment = etree.SubElement(description, "Comment")
- comment.text = config_dict["Description"]["Comment"]
+ if "gb-comment" in metadata and pd.notnull(metadata["gb-comment"].iloc[0]) and metadata["gb-comment"].iloc[0].strip() != "":
+ comment.text = metadata["gb-comment"].iloc[0]
+ else:
+ comment.text = "GenBank Submission"
# Description info including organization and contact info
organization = etree.SubElement(description, "Organization", type=config_dict["Description"]["Organization"]["Type"], role=config_dict["Description"]["Organization"]["Role"])
org_name = etree.SubElement(organization, "Name")
@@ -211,6 +217,8 @@ def create_authorset(config_dict: Dict[str, Any], metadata: pd.DataFrame, submis
# Create a zip file for genbank submission
def create_files(organism: str, config_dict: Dict[str, Any], metadata: pd.DataFrame, submission_name: str, submission_dir: str, gff_file: Optional[str]) -> None:
+ # Drop submission xml columns
+ metadata = metadata.drop(columns=["gb-title", "gb-comment"], errors="ignore")
# Create authorset file
create_authorset(config_dict=config_dict, metadata=metadata, submission_name=submission_name, submission_dir=submission_dir)
file_handler.create_fasta(database="GENBANK", metadata=metadata, submission_dir=submission_dir)
diff --git a/gisaid_cli/fluCLI/fluCLI b/gisaid_cli/fluCLI/fluCLI
deleted file mode 100644
index e69de29..0000000
diff --git a/gisaid_handler.py b/gisaid_handler.py
index 06e65fc..bcf19fb 100755
--- a/gisaid_handler.py
+++ b/gisaid_handler.py
@@ -6,7 +6,7 @@
import shutil
import subprocess
-from typing import Dict, Any, List
+from typing import Dict, Any, List, Optional, Match
import os
import pandas as pd
import file_handler
@@ -26,17 +26,18 @@ def create_gisaid_files(organism: str, database: str, submission_name: str, subm
# Get column names for gisaid submission only
gisaid_df = metadata.filter(regex=GISAID_REGEX).copy()
gisaid_df.columns = gisaid_df.columns.str.replace("gs-","").str.strip()
- #Add required GISAID fields
+ # Add required GISAID fields
+ # covCLI returns an error when authors or collection_date are capitalized
if organism in ["COV", "POX", "ARBO"]:
- gisaid_df = gisaid_df.rename(columns = {"sample_name": "virus_name", "authors": "Authors", "collection_date": "Collection_Date"})
if organism == "COV":
- prefix_name = "covv_"
+ sample_name_column = "covv_virus_name"
else:
- prefix_name = organism.lower() + "_"
- gisaid_df = gisaid_df.add_prefix(prefix_name)
+ sample_name_column = organism.lower() + "_virus_name"
+ gisaid_df = gisaid_df.rename(columns = {"sample_name": sample_name_column})
gisaid_df["submitter"] = config_dict["Username"]
- gisaid_df["fn"] = ""
- first_cols = ["submitter", "fn", (prefix_name + "virus_name")]
+ # fn field is for fasta file name
+ gisaid_df["fn"] = "sequence.fsa"
+ first_cols = ["submitter", "fn", sample_name_column]
elif "FLU" in organism:
gisaid_df = gisaid_df.rename(columns = {"authors": "Authors", "collection_date": "Collection_Date"})
gisaid_df["Isolate_Id"] = ""
@@ -68,21 +69,55 @@ def process_gisaid_log(log_file: str, submission_dir: str) -> pd.DataFrame:
while line:
# If accession generated record it
# Pattern options: "msg:": "; _" OR : ; _
- if re.match("(?i)(\W|^)(\"msg\":\s*\"\S+.*;\s*(EPI_ISL|EPI_ID)_\d{6,}\"|(epi_id|epi_isl_id):\s*\S.*;\s*(EPI_ISL_|EPI)\d+)(\W|$)", line):
- gisaid_string = re.sub("(\W|^)\"msg\":\s*\"", "", line)
- gisaid_string_list: List[str] = gisaid_string.strip().replace("\"", "").split(";")
- sample_name = re.sub("(epi_isl_id|epi_id):\s*", "", gisaid_string_list[0].strip())
- accession = gisaid_string_list[1].strip()
- if re.match("EPI_ISL_\d+", accession):
- gisaid_isolate_log.append({"gs-sample_name":sample_name, "gisaid_accession_epi_isl_id":accession})
- elif re.match("EPI\d+", accession):
- gisaid_segment_log.append({"gs-segment_name":sample_name, "gisaid_accession_epi_id":accession})
+ if re.search("(?i)(\W|^)(\"msg\":\s*\"\S+.*;\s*(EPI_ISL|EPI_ID)_\d*\"|(epi_id|epi_isl_id):\s*\S.*;\s*(EPI_ISL_|EPI)\d+)(\W|$)", line):
+ gisaid_string_search = re.findall(r'(?:[a-zA-Z0-9_-]+(?:/[a-zA-Z0-9_-]+)+|EPI_\w*)', line)
+ gisaid_string = ' '.join(gisaid_string_search)
+ gisaid_string_list: List[str] = gisaid_string.split(' ')
+ sample_name = gisaid_string_list[0].strip()
+ accession_string = gisaid_string_list[1].strip()
+ if re.match("EPI_ISL_\d+", accession_string):
+ gisaid_isolate_log.append({"gs-sample_name":sample_name, "gisaid_accession_epi_isl_id":accession_string})
+ elif re.match("EPI\d+", accession_string):
+ gisaid_segment_log.append({"gs-segment_name":sample_name, "gisaid_accession_epi_id":accession_string})
+ # Handling if submitting samples have already been registered in GISAID
+ elif re.search(r'"code":\s*"validation_error".*?already exists;\s*existing_virus_name:', line):
+ sample_name_search = re.search(r"(hCoV[^;]+);", line)
+ if sample_name_search:
+ sample_name = sample_name_search.group(1)
+ if re.search(r"\['(EPI_ISL_\d+)'\]", line):
+ accession_search = re.search(r"\['(EPI_ISL_\d+)'\]", line)
+ if accession_search:
+ accession = accession_search.group(1)
+ else:
+ accession = ""
+ gisaid_isolate_log.append({"gs-sample_name":sample_name, "gisaid_accession_epi_isl_id":accession})
+ elif re.search(r"\['(EPI_\d+)'\]", line):
+ accession_search = re.search(r"\['(EPI_\d+)'\]", line)
+ if accession_search:
+ accession = accession_search.group(1)
+ else:
+ accession = ""
+ gisaid_segment_log.append({"gs-segment_name":sample_name, "gisaid_accession_epi_id":accession})
+ else:
+ print("Finished reading GISAID log. If workflow has failed here, it's likely no GISAID IDs were returned. Check results in GISAID upload log.")
line = file.readline().strip()
gisaid_isolate_df = pd.DataFrame(gisaid_isolate_log)
gisaid_segment_df = pd.DataFrame(gisaid_segment_log)
- upload_log.update_submission_status_csv(submission_dir=submission_dir.replace("/GISAID", "/"), update_database="GISAID", update_df=gisaid_isolate_df)
- upload_log.update_submission_status_csv(submission_dir=submission_dir.replace("/GISAID", "/"), update_database="GISAID", update_df=gisaid_segment_df)
- gisaid_isolate_df = gisaid_isolate_df[~gisaid_isolate_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_\d{6,}", regex = True, na = False)].copy()
+ # Update GISAID submission status
+ if not gisaid_isolate_df.empty and not gisaid_segment_df.empty:
+ print("GISAID isolates and GISAID segments found.")
+ upload_log.update_submission_status_csv(submission_dir=submission_dir, update_database="GISAID", update_df=gisaid_isolate_df)
+ upload_log.update_submission_status_csv(submission_dir=submission_dir, update_database="GISAID", update_df=gisaid_segment_df)
+ elif not gisaid_isolate_df.empty:
+ print("GISAID isolates found.")
+ upload_log.update_submission_status_csv(submission_dir=submission_dir, update_database="GISAID", update_df=gisaid_isolate_df)
+ elif not gisaid_segment_df.empty:
+ print("GISAID segments found.")
+ upload_log.update_submission_status_csv(submission_dir=submission_dir, update_database="GISAID", update_df=gisaid_segment_df)
+ else:
+ print("Warning: no GISAID isolates or segments found")
+ gisaid_isolate_df = gisaid_isolate_df[~gisaid_isolate_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_\d*", regex = True, na = False)].copy()
+ gisaid_isolate_df = gisaid_isolate_df[~gisaid_isolate_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_\d*", regex = True, na = False)].copy()
return gisaid_isolate_df[["gs-sample_name"]]
# Submit to GISAID
@@ -92,15 +127,17 @@ def submit_gisaid(organism: str, submission_dir: str, submission_name: str, conf
orig_metadata = os.path.join(submission_dir, "orig_metadata.csv")
fasta = os.path.join(submission_dir, "sequence.fsa")
orig_fasta = os.path.join(submission_dir, "orig_sequence.fsa")
+ submission_status_file = os.path.join(os.path.dirname(submission_dir), "submission_status_report.csv")
# Extract user credentials (e.g. username, password, client-id)
tools.check_credentials(config_dict=config_dict, database="GISAID")
gisaid_cli = file_handler.validate_gisaid_installer(submission_dir=submission_dir, organism=organism)
print(f"Uploading sample files to GISAID-{organism}, as a '{submission_type}' submission. If this is not intended, interrupt immediately.", file=sys.stdout)
time.sleep(5)
# Set number of attempt to 3 if erroring out occurs
- attempts = 1
+ attempts = 0
# Submit to GISAID
while attempts <= 3:
+ attempts += 1
print("\n"+"Submission attempt: " + str(attempts), file=sys.stdout)
# Create a log submission for each attempt
log_file = os.path.join(submission_dir, "gisaid_upload_log_" + str(attempts) + ".txt")
@@ -120,34 +157,50 @@ def submit_gisaid(organism: str, submission_dir: str, submission_name: str, conf
while not os.path.exists(log_file):
time.sleep(10)
# Check submission log to see if all samples are uploaded successfully
- not_submitted_df = process_gisaid_log(log_file=log_file, submission_dir=submission_dir)
- # If submission completed, no more attempts
- if not_submitted_df.empty:
+ process_gisaid_log(log_file=log_file, submission_dir=submission_dir)
+ # Read in the submission status report
+ status_df = pd.read_csv(submission_status_file, header = 0, dtype = str, engine = "python", encoding="utf-8", index_col=False)
+ # Gather all required files
+ metadata = os.path.join(submission_dir, "metadata.csv")
+ fasta = os.path.join(submission_dir, "sequence.fsa")
+ # Filter out samples with accession
+ if "FLU" in organism:
+ metadata_column_name = "Isolate_Name"
+ fasta_column_name = "gs-segment_name"
+ gisaid_status_df = status_df[~status_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_", na=False) & ~status_df["gisaid_accession_epi_id"].str.contains("EPI", na=False)].copy()
+ gisaid_status_df = gisaid_status_df[["gs-sample_name", "gs-segment_name"]]
+ elif "COV" in organism:
+ metadata_column_name = "covv_virus_name"
+ fasta_column_name = "gs-sample_name"
+ gisaid_status_df = status_df[~status_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_", na=False)].copy()
+ gisaid_status_df = gisaid_status_df[["gs-sample_name"]]
+ else:
+ metadata_column_name = organism.lower() + "_virus_name"
+ fasta_column_name = "gs-sample_name"
+ gisaid_status_df = status_df[~status_df["gisaid_accession_epi_isl_id"].str.contains("EPI_ISL_", na=False)].copy()
+ gisaid_status_df = gisaid_status_df[["gs-sample_name"]]
+ # Identify remaining samples
+ metadata_df = pd.read_csv(orig_metadata, header = 0, dtype = str, engine = "python", encoding="utf-8", index_col=False)
+ metadata_df = metadata_df.merge(gisaid_status_df, how="inner", left_on=metadata_column_name, right_on="gs-sample_name")
+ if metadata_df.empty:
print("Uploading successfully", file=sys.stdout)
print("Log file is stored at: " + submission_dir + "/gisaid_upload_log_attempt_" + str(attempts) + ".txt", file=sys.stdout)
return "PROCESSED"
- else:
- # If submission is not completed, try again
- metadata_df = pd.read_csv(metadata, header = 0, dtype = str, engine = "python", encoding="utf-8", index_col=False)
- if "FLU" in organism:
- column_name = "Isolate_Name"
- elif "COV" in organism:
- column_name = "virus_name"
- metadata_df = metadata_df.merge(not_submitted_df, how="inner", left_on=column_name, right_on="gs-sample_name")
- fasta_names = metadata_df["gs-sequence_name"].tolist()
- metadata_df = metadata_df.drop(columns=["gs-sample_name", "gs-sequence_name"])
- metadata_df.to_csv(orig_metadata, header = True, index = False)
- fasta_dict = []
- with open(orig_fasta, "r") as fsa:
- records = SeqIO.parse(fsa, "fasta")
- for record in records:
- if record.id in fasta_names:
- fasta_dict.append(record)
- with open(fasta, "w+") as fasta_file:
- SeqIO.write(fasta_dict, fasta_file, "fasta")
- attempts += 1
- if not not_submitted_df.empty:
- print("Error: " + str(len(not_submitted_df.index)) + " sample(s) failed to upload to GISAID", file=sys.stderr)
+ # Update metadata file
+ fasta_names = gisaid_status_df[fasta_column_name].tolist()
+ metadata_df = metadata_df.drop(columns=["gs-sample_name", "gs-segment_name"], errors="ignore")
+ metadata_df.to_csv(metadata, header = True, index = False)
+ # Update fasta file
+ fasta_dict = []
+ with open(orig_fasta, "r") as fsa:
+ records = SeqIO.parse(fsa, "fasta")
+ for record in records:
+ if record.id in fasta_names:
+ fasta_dict.append(record)
+ with open(fasta, "w+") as fasta_file:
+ SeqIO.write(fasta_dict, fasta_file, "fasta")
+ if not metadata_df.empty:
+ print("Error: " + str(len(metadata_df.index)) + " sample(s) failed to upload to GISAID", file=sys.stderr)
print("Please check log file at: " + submission_dir + "/gisaid_upload_log_attempt_{1,2,3}.txt", file=sys.stderr)
return "ERROR"
else:
@@ -163,16 +216,23 @@ def update_gisaid_files(organism: str, submission_dir: str, submission_status_fi
orig_fasta = os.path.join(submission_dir, "orig_sequence.fsa")
# Filter out genbank that has accession number
genbank_status_df = status_df[status_df["genbank-status"].str.contains("processed-ok", na=False)].copy()
- gisaid_status_df = genbank_status_df[["gs-sample_name", "gs-sequence_name"]]
# Add required gisaid fields
metadata_df = pd.read_csv(metadata, header = 0, dtype = str, engine = "python", encoding="utf-8", index_col=False)
if "FLU" in organism:
- column_name = "Isolate_Name"
+ metadata_column_name = "Isolate_Name"
+ fasta_column_name = "gs-segment_name"
+ gisaid_status_df = genbank_status_df[["gs-sample_name", "gs-segment_name"]]
elif "COV" in organism:
- column_name = "virus_name"
- metadata_df = metadata_df.merge(gisaid_status_df, how="inner", left_on=column_name, right_on="gs-sample_name")
- fasta_names = metadata_df["gs-sequence_name"].tolist()
- metadata_df = metadata_df.drop(columns=["gs-sample_name", "gs-sequence_name"])
+ metadata_column_name = "covv_virus_name"
+ fasta_column_name = "gs-sample_name"
+ gisaid_status_df = genbank_status_df[["gs-sample_name"]]
+ else:
+ metadata_column_name = organism.lower() + "_virus_name"
+ fasta_column_name = "gs-sample_name"
+ gisaid_status_df = genbank_status_df[["gs-sample_name"]]
+ metadata_df = metadata_df.merge(gisaid_status_df, how="inner", left_on=metadata_column_name, right_on="gs-sample_name")
+ fasta_names = metadata_df[fasta_column_name].tolist()
+ metadata_df = metadata_df.drop(columns=["gs-sample_name", "gs-segment_name"], errors="ignore")
metadata_df.to_csv(orig_metadata, header = True, index = False)
fasta_dict = []
with open(orig_fasta, "r") as fsa:
diff --git a/ncbi_handler.py b/ncbi_handler.py
index 0b012b7..72e0d12 100644
--- a/ncbi_handler.py
+++ b/ncbi_handler.py
@@ -127,9 +127,6 @@ def submit_ncbi(database: str, submission_name: str, submission_dir: str, config
tools.check_credentials(config_dict=config_dict, database="NCBI")
# Submit sequences to NCBI via FTP Server
print(f"Uploading sample files to NCBI-{database}, as a '{submission_type}' submission. If this is not intended, interrupt immediately.", file=sys.stdout)
- if submission_type != "TEST":
- print("Error: submission_type " + str(submission_type))
- sys.exit(1)
time.sleep(5)
try:
# Login into NCBI FTP Server
diff --git a/seqsender.py b/seqsender.py
index 33e8aac..82fba53 100755
--- a/seqsender.py
+++ b/seqsender.py
@@ -25,6 +25,8 @@
from settings import VERSION
+import sys
+
# Define current time
STARTTIME = datetime.now()
@@ -46,6 +48,9 @@ def prep(database: List[str], organism: str, submission_dir: str, submission_nam
file_handler.validate_directory(name = "submission directory", path = submission_dir)
# Validate files
for file_type, file_path in file_dict.items():
+ if file_type == "fasta_file" and file_path is None and ("GENBANK" in database or "GISAID" in database):
+ print("Error: Submitting to GenBank or GISAID requires a fasta file for submission. Add a fasta file to your submission with the flag '--fasta_file'. ", file=sys.stderr)
+ sys.exit(1)
if file_type in ["fasta_file", "gff_file"] and file_path is None:
# If not provided
continue
@@ -60,6 +65,12 @@ def prep(database: List[str], organism: str, submission_dir: str, submission_nam
file_dict[file_type] = updated_path # type: ignore
# load config file
config_dict = tools.get_config(config_file=file_dict["config_file"], database=database)
+ # Warn user if submitting biosample & sra together with 'Link_Sample_Between_NCBI_Databases' set to False
+ if not config_dict["NCBI"]["Link_Sample_Between_NCBI_Databases"] and "SRA" in database and "BIOSAMPLE" in database:
+ print("Warning: You are submitting to BioSample and SRA together, and your config has the field 'Link_Sample_Between_NCBI_Databases', turned off. Your BioSample and SRA submission will still be linked together as this is required for submitting to SRA.")
+ # Warn user if submitting to only SRA, they still require a BioSample submission
+ if "SRA" in database and "BIOSAMPLE" not in database:
+ print("Warning: You are submitting to SRA but not to BioSample. SRA requires a BioSample submission when submitting raw reads. Ensure you also make a BioSample submission to prevent submission errors.")
# load metadata file
metadata = tools.get_metadata(database=database, organism=organism, metadata_file=file_dict["metadata_file"], config_dict=config_dict, skip_validation=skip_validation)
# Load fasta file into metadata
@@ -117,7 +128,7 @@ def submit(database: List[str], organism: str, submission_dir: str, submission_n
submission_status = "SUBMITTED"
elif "GISAID" in database_name:
sub_pos = tools.get_submission_position(config_dict=config_dict, database="GISAID")
- if sub_pos is None or sub_pos == 1:
+ if sub_pos is None or sub_pos == 1 or "GENBANK" not in database:
submission_status = gisaid_handler.submit_gisaid(organism=organism, submission_dir=database_dir, submission_name=submission_name, config_dict=config_dict["GISAID"], submission_type=submission_type)
else:
print(f"Error: Database selection {database_name} is not valid.", file=sys.stderr)
@@ -143,7 +154,6 @@ def main():
if args.genbank:
database += [args.genbank]
if args.gisaid:
- print("here")
database += [args.gisaid]
if len(database) == 0:
print("ERROR: Missing a required database selection. See USAGE below.", file=sys.stderr)
@@ -163,7 +173,7 @@ def main():
elif command == "test_data":
setup.create_test_data(organism=args.organism, database=database, submission_dir=submission_dir)
elif command == "version":
- print("Version: {VERSION}", file=sys.stdout)
+ print(f"Version: {VERSION}", file=sys.stdout)
elif command == "update_biosample":
print("Updating BioSample requirements.", file=sys.stdout)
setup.download_biosample_xml_list()
diff --git a/settings.py b/settings.py
index 73d9278..b49d6e6 100755
--- a/settings.py
+++ b/settings.py
@@ -12,7 +12,7 @@
PROG_DIR: str = os.path.dirname(os.path.abspath(__file__))
# SeqSender version
-VERSION: str = "1.1.0 (Beta)"
+VERSION: str = "1.2.1 (Beta)"
# Organism options with unique submission options
ORGANISM_CHOICES: List[str] = ["FLU", "COV", "POX", "ARBO", "OTHER"]
@@ -33,7 +33,7 @@
SUBMISSION_LOG_COLUMNS: List[str] = ["Submission_Name", "Organism", "Database", "Submission_Type", "Submission_Date", "Submission_ID", "Submission_Status", "Submission_Directory", "Config_File", "Update_Date"]
# Shiny schema options, exclusion list
-SCHEMA_EXCLUSIONS = ["config.seqsender.upload_log_schema","config.seqsender.config_file.gisaid_schema","config.seqsender.config_file.ncbi_gisaid_schema", "config.seqsender.config_file.ncbi_schema"]
+SCHEMA_EXCLUSIONS = ["config.seqsender.upload_log_schema","config_file.ncbi_schema","config_file.ncbi_gisaid_schema", "config_file.gisaid_schema"]
##### NCBI settings #####
# FTP website to submit samples to
@@ -49,7 +49,7 @@
BIOSAMPLE_REGEX = "^bs-|^bioproject$|^organism$|^collection_date$"
# SRA metadata regex
-SRA_REGEX = "^sra-|^bioproject$|^organism$|^collection_date$"
+SRA_REGEX = "^sra-|^bioproject$|bs-sample_name|^organism$|^collection_date$"
# Genbank metadata regex
GENBANK_REGEX = "^gb-sample_name$"
@@ -62,4 +62,4 @@
##### GISAID settings #####
# GISAID metadata regex
-GISAID_REGEX = "^gs-|^collection_date$|^authors"
+GISAID_REGEX = "^gs-|^collection_date$|^authors$"
diff --git a/setup.py b/setup.py
index b21075c..2317f55 100644
--- a/setup.py
+++ b/setup.py
@@ -22,6 +22,7 @@
# Local imports
sys.path.insert(0, str(pathlib.Path(__file__).parent))
+import tools
# Get program directory
PROG_DIR: str = os.path.dirname(os.path.abspath(__file__))
@@ -45,7 +46,52 @@
required=True,
description=\"Identifier name used for BioSample. Max length is 50 characters.\",
title=\"sample_name\",
+ ),
+ \"bs-sample_title\": Column(
+ dtype=\"object\",
+ checks=None,
+ nullable=True,
+ unique=False,
+ coerce=False,
+ required=False,
+ description=\"Optional additional title for sample. Will be autogenerated by NCBI if not provided.\",
+ title=\"sample title\",
+ ),
+ \"bs-sample_description\": Column(
+ dtype=\"object\",
+ checks=None,
+ nullable=True,
+ unique=False,
+ coerce=False,
+ required=False,
+ description=\"Optional description for sample.\",
+ title=\"sample description\",
),"""
+SCHEMA_COLUMNS_FOOTER: str = """
+ \"bs-title\": Column(
+ dtype=\"object\",
+ checks=[
+ Check(lambda s: s.nunique() == 1),
+ ],
+ nullable=True,
+ unique=False,
+ coerce=False,
+ required=False,
+ description=\"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix \\\"-BS\\\" will be used instead.\",
+ title=\"biosample submission portal name\",
+ ),
+ \"bs-comment\": Column(
+ dtype=\"object\",
+ checks=[
+ Check(lambda s: s.nunique() == 1),
+ ],
+ nullable=True,
+ unique=False,
+ coerce=False,
+ required=False,
+ description=\"Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.\",
+ title=\"biosample submission portal description\",
+ )"""
# Create example data for testing
def create_test_data(organism: str, database: List[str], submission_dir: str) -> None:
@@ -77,7 +123,7 @@ def create_test_data(organism: str, database: List[str], submission_dir: str) ->
print("\n"+"Generating submission test_data", file=sys.stdout)
# Get combined metadata for all given databases
database_prefix = {"GENBANK": "gb-", "GISAID": "gs-", "SRA": "sra-", "BIOSAMPLE": "bs-"}
- repeat_columns = ["sample_name", "sequence_name", "collection_date", "organism", "authors", "bioproject"]
+ repeat_columns = ["sample_name", "sequence_name", "collection_date", "organism", "authors", "bioproject", "bs-sample_name"]
for i in range(len(database)):
df = pd.read_csv(os.path.join(PROG_DIR, "test_data", organism, organism.lower()+"_"+database[i].lower()+"_metadata.csv"), header = 0, dtype = str, engine = "python", encoding="utf-8", index_col=False, na_filter=False)
if i == 0:
@@ -163,6 +209,7 @@ def download_biosample_xml_list() -> None:
print(error, file=sys.stderr)
time.sleep(5)
line = file.readline()
+ tools.update_all_schema_templates()
# Convert downloaded BioSample package xml to Pandera Schema
def biosample_package_to_pandera_schema(xml_file: str, name: str) -> None:
@@ -226,6 +273,7 @@ def biosample_package_to_pandera_schema(xml_file: str, name: str) -> None:
indentation = indentation[:-1]
file.write(indentation + "),")
# Close columns
+ file.write(SCHEMA_COLUMNS_FOOTER)
indentation = indentation[:-1]
file.write(indentation + "},")
# Create dataframe wide checks
@@ -248,7 +296,7 @@ def biosample_package_to_pandera_schema(xml_file: str, name: str) -> None:
file.write(indentation + "unique=None,")
file.write(indentation + "report_duplicates=\"all\",")
file.write(indentation + "unique_column_names=True,")
- file.write(indentation + "add_missing_columns=True,")
+ file.write(indentation + "add_missing_columns=False,")
file.write(indentation + "title=\"BioSample package " + name + " schema\",")
file.write(indentation + "description=\"Schema validation for BioSample database using " + name + " package.\",")
# Close schema
diff --git a/shiny/app.py b/shiny/app.py
index b715403..e16957c 100755
--- a/shiny/app.py
+++ b/shiny/app.py
@@ -20,7 +20,7 @@
header = (
ui.card_header(
ui.HTML(
- """Beta Version: 1.2.0. This pipeline is currently in Beta testing, and issues could appear during submission. Please use it at your own risk. Feedback and suggestions are welcome!
"""
+ """Beta Version: 1.2.1. This pipeline is currently in Beta testing, and issues could appear during submission. Please use it at your own risk. Feedback and suggestions are welcome!
"""
)
),
)
@@ -648,31 +648,64 @@ def read_gisaid_file():
def BioSample_Package_Name():
return input.BioSample_packages()
+ @reactive.effect
+ @reactive.event(input.SRA_checkbox)
+ def sra_submission_requires_biosample():
+ if input.SRA_checkbox() == True and input.BioSample_checkbox() == False:
+ with reactive.isolate():
+ ui.update_checkbox("BioSample_checkbox", value = True)
+
+
@reactive.effect
@reactive.event(input.ncbi_submission_position)
def gisaid_submission_position():
- if "First" in input.ncbi_submission_position():
- value = "Second"
+ if input.ncbi_submission_position() == "1":
+ value = "2"
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "gisaid_submission_position",
+ selected=value,
+ )
+ elif input.ncbi_submission_position() == "2":
+ value = "1"
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "gisaid_submission_position",
+ selected=value,
+ )
else:
- value = "First"
- with reactive.isolate():
- ui.update_radio_buttons(
- "gisaid_submission_position",
- selected=value,
- )
+ value = ""
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "gisaid_submission_position",
+ selected=value,
+ )
@reactive.effect
@reactive.event(input.gisaid_submission_position)
def ncbi_submission_position():
- if "First" in input.gisaid_submission_position():
- value = "Second"
+ if input.gisaid_submission_position() == "1":
+ value = "2"
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "ncbi_submission_position",
+ selected=value,
+ )
+ elif input.gisaid_submission_position() == "2":
+ value = "1"
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "ncbi_submission_position",
+ selected=value,
+ )
else:
- value = "First"
- with reactive.isolate():
- ui.update_radio_buttons(
- "ncbi_submission_position",
- selected=value,
- )
+ value = ""
+ with reactive.isolate():
+ ui.update_radio_buttons(
+ "ncbi_submission_position",
+ selected=value,
+ )
+
@reactive.Calc
def initialize_base_dataframe():
@@ -749,8 +782,6 @@ def initialize_config():
"Specified_Release_Date": input.ncbi_config_release_date() or "",
"Link_Sample_Between_NCBI_Databases": input.ncbi_config_link_samples() or "",
"Description": {
- "Title": input.ncbi_config_title() or "",
- "Comment": input.ncbi_config_comment() or "",
"Organization": {
"Role": input.ncbi_config_role() or "",
"Type": input.ncbi_config_type() or "",
diff --git a/shiny/setup.py b/shiny/setup.py
index 9510aa4..2ca3dea 100755
--- a/shiny/setup.py
+++ b/shiny/setup.py
@@ -22,10 +22,12 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
position="right",
),
),
- ui.input_checkbox("BioSample_checkbox", "BioSample", width=None, value=True),
- ui.input_checkbox("SRA_checkbox", "SRA", width=None),
+ ui.input_checkbox("BioSample_checkbox", "BioSample", width=None),
+ ui.tooltip(ui.input_checkbox("SRA_checkbox", "SRA", width=None),
+ "When submitting to SRA, a BioSample submission is also required.",
+ style="display:inline-block;float:right;"),
ui.input_checkbox("GenBank_checkbox", "GenBank", width=None),
- ui.input_checkbox("GISAID_checkbox", "GISAID", width=None, value=True),
+ ui.input_checkbox("GISAID_checkbox", "GISAID", width=None),
),
ui.card(
ui.card_header(
@@ -53,7 +55,7 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
"MISAG": {"MISAG.6.0":"MISAG.6.0","MISAG.agriculture.6.0":"MISAG.agriculture.6.0","MISAG.air.6.0":"MISAG.air.6.0","MISAG.built.6.0":"MISAG.built.6.0","MISAG.food-animal.6.0":"MISAG.food-animal.6.0","MISAG.food-farm.env.6.0":"MISAG.food-farm.env.6.0","MISAG.food-human.foods.6.0":"MISAG.food-human.foods.6.0","MISAG.food-prod.facility.6.0":"MISAG.food-prod.facility.6.0","MISAG.host-associated.6.0":"MISAG.host-associated.6.0","MISAG.human-associated.6.0":"MISAG.human-associated.6.0","MISAG.human-gut.6.0":"MISAG.human-gut.6.0","MISAG.human-oral.6.0":"MISAG.human-oral.6.0","MISAG.human-skin.6.0":"MISAG.human-skin.6.0","MISAG.human-vaginal.6.0":"MISAG.human-vaginal.6.0","MISAG.hydrocarbon-cores.6.0":"MISAG.hydrocarbon-cores.6.0","MISAG.hydrocarbon-fluids.swabs.6.0":"MISAG.hydrocarbon-fluids.swabs.6.0","MISAG.microbial.6.0":"MISAG.microbial.6.0","MISAG.miscellaneous.6.0":"MISAG.miscellaneous.6.0","MISAG.plant-associated.6.0":"MISAG.plant-associated.6.0","MISAG.sediment.6.0":"MISAG.sediment.6.0","MISAG.soil.6.0":"MISAG.soil.6.0","MISAG.symbiont-associated.6.0":"MISAG.symbiont-associated.6.0","MISAG.wastewater.6.0":"MISAG.wastewater.6.0","MISAG.water.6.0":"MISAG.water.6.0"},
"MIUVIG": {"MIUVIG.6.0":"MIUVIG.6.0","MIUVIG.agriculture.6.0":"MIUVIG.agriculture.6.0","MIUVIG.air.6.0":"MIUVIG.air.6.0","MIUVIG.built.6.0":"MIUVIG.built.6.0","MIUVIG.food-animal.6.0":"MIUVIG.food-animal.6.0","MIUVIG.food-farm.env.6.0":"MIUVIG.food-farm.env.6.0","MIUVIG.food-human.foods.6.0":"MIUVIG.food-human.foods.6.0","MIUVIG.food-prod.facility.6.0":"MIUVIG.food-prod.facility.6.0","MIUVIG.host-associated.6.0":"MIUVIG.host-associated.6.0","MIUVIG.human-associated.6.0":"MIUVIG.human-associated.6.0","MIUVIG.human-gut.6.0":"MIUVIG.human-gut.6.0","MIUVIG.human-oral.6.0":"MIUVIG.human-oral.6.0","MIUVIG.human-skin.6.0":"MIUVIG.human-skin.6.0","MIUVIG.human-vaginal.6.0":"MIUVIG.human-vaginal.6.0","MIUVIG.hydrocarbon-cores.6.0":"MIUVIG.hydrocarbon-cores.6.0","MIUVIG.hydrocarbon-fluids.swabs.6.0":"MIUVIG.hydrocarbon-fluids.swabs.6.0","MIUVIG.microbial.6.0":"MIUVIG.microbial.6.0","MIUVIG.miscellaneous.6.0":"MIUVIG.miscellaneous.6.0","MIUVIG.plant-associated.6.0":"MIUVIG.plant-associated.6.0","MIUVIG.sediment.6.0":"MIUVIG.sediment.6.0","MIUVIG.soil.6.0":"MIUVIG.soil.6.0","MIUVIG.symbiont-associated.6.0":"MIUVIG.symbiont-associated.6.0","MIUVIG.wastewater.6.0":"MIUVIG.wastewater.6.0","MIUVIG.water.6.0":"MIUVIG.water.6.0"}
},
- selected="SARS",
+ selected="Pathogen.cl.1.0",
),
),
# If GenBank checkbox checked then load Genbank Schema Options
@@ -61,8 +63,9 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
"input.GenBank_checkbox",
ui.input_select(
"GenBank_schemas",
- label="Select GenBank Schema:",
+ label="Select SeqSender GenBank Schema:",
choices=["COV", "FLU", "OTHER"],
+ selected="OTHER",
),
),
# If GISAID checkbox checked then load GISAID Database Options
@@ -71,7 +74,7 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
ui.input_select(
"GISAID_databases",
label="Select GISAID Database:",
- choices=["COV", "FLU", "POX", "ARBO"],
+ choices=["FLU", "COV", "POX", "ARBO"],
),
),
),
@@ -154,7 +157,8 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
div(ui.input_radio_buttons(
"ncbi_submission_position",
label=None,
- choices=["First", "Second", "None"],
+ choices={1:"1", 2:"2", "":"None"},
+ selected=1,
inline=True,
),
style="display:inline-block;height:5px;font-size:medium;",
@@ -180,14 +184,6 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
div(ui.HTML("
"), style="margin-top:-25px;"),
# Description category
config_indent(2, "Description:", custom_style = ""),
- # Project Title
- config_indent(3, "Title:"),
- config_text_input("ncbi_config_title", placeholder = "Submission Title", help_msg = "Descriptive name for the samples you'll be submitting with this config file. (i.e. my flu project, my lab's flu submissions)"),
- div(ui.HTML("
"), style="margin-top:-25px;"),
- # Project Description / Comment
- config_indent(3, "Comment:"),
- config_text_input("ncbi_config_comment", placeholder = "Submission Comment", help_msg = "Description of the samples you'll be submitting with this config file. (i.e. wastewater surveillance)"),
- div(ui.HTML("
"), style="margin-top:-25px;"),
# Organization category
config_indent(3, "Organization", custom_style = ""),
# Organization role
@@ -277,9 +273,9 @@ def config_text_input(id, placeholder, help_msg, custom_style = "display:inline-
div(ui.input_radio_buttons(
"gisaid_submission_position",
label=None,
- choices=["First", "Second", "None"],
+ choices={1:"1", 2:"2", "":"None"},
inline=True,
- selected="Second",
+ selected=2,
),
style="display:inline-block;height:5px;font-size:medium;",
),
diff --git a/shiny/templates/config.biosample.Beta-lactamase.1.0_template.csv b/shiny/templates/config.biosample.Beta-lactamase.1.0_template.csv
index d38920f..9b619ec 100644
--- a/shiny/templates/config.biosample.Beta-lactamase.1.0_template.csv
+++ b/shiny/templates/config.biosample.Beta-lactamase.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-beta_lactamase_family,Required,Specify the beta-lactamase family for this gene.
@@ -11,3 +13,5 @@ bs-host,Optional,"The natural (as opposed to laboratory) host to the organism fr
bs-isolation_source,Optional,"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
bs-lab_host,Optional,"Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained, e.g., Escherichia coli DH5a, or Homo sapiens HeLa cells"
bs-lat_lon,Optional,"The geographical coordinates of the location where the sample was collected. Specify as degrees latitude and longitude in format ""d[d.dddd] N|S d[dd.dddd] W|E"", eg, 38.98 N 77.11 W"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Human.1.0_template.csv b/shiny/templates/config.biosample.Human.1.0_template.csv
index 570c004..172076b 100644
--- a/shiny/templates/config.biosample.Human.1.0_template.csv
+++ b/shiny/templates/config.biosample.Human.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-age,Required,"age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees"
bs-biomaterial_provider,Required,"name and address of the lab or PI, or a culture collection identifier"
@@ -21,3 +23,5 @@ bs-population,Optional,"for human: ; for plants: filial generation, number of pr
bs-race,Optional,
bs-sample_type,Optional,"Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly"
bs-treatment,Optional,
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Invertebrate.1.0_template.csv b/shiny/templates/config.biosample.Invertebrate.1.0_template.csv
index 9dd1df5..5a90068 100644
--- a/shiny/templates/config.biosample.Invertebrate.1.0_template.csv
+++ b/shiny/templates/config.biosample.Invertebrate.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-breed,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". breed name - chiefly used in domesticated animals or plants"
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
@@ -19,3 +21,5 @@ bs-lat_lon,Optional,"The geographical coordinates of the location where the samp
bs-sex,Optional,physical sex of sampled organism
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.6.0_template.csv
index 607ac2a..226fe8c 100644
--- a/shiny/templates/config.biosample.MIGS.ba.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -30,3 +32,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.agriculture.6.0_template.csv
index 52af95b..1d2cf6a 100644
--- a/shiny/templates/config.biosample.MIGS.ba.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -152,3 +154,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.air.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.air.6.0_template.csv
index 88841af..de6d481 100644
--- a/shiny/templates/config.biosample.MIGS.ba.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -56,3 +58,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.built.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.built.6.0_template.csv
index fbfa89c..8c3c921 100644
--- a/shiny/templates/config.biosample.MIGS.ba.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
bs-air_temp,Required,temperature of the air at the time of sampling
@@ -190,3 +192,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.food-animal.6.0_template.csv
index f81f062..9187fa5 100644
--- a/shiny/templates/config.biosample.MIGS.ba.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -118,3 +120,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.food-farm.env.6.0_template.csv
index 3bd0c93..a8760fd 100644
--- a/shiny/templates/config.biosample.MIGS.ba.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
@@ -156,3 +158,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.food-human.foods.6.0_template.csv
index e187435..6657c15 100644
--- a/shiny/templates/config.biosample.MIGS.ba.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -124,3 +126,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.food-prod.facility.6.0_template.csv
index 2628c6e..5398773 100644
--- a/shiny/templates/config.biosample.MIGS.ba.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -114,3 +116,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.host-associated.6.0_template.csv
index 2827ebb..ec06818 100644
--- a/shiny/templates/config.biosample.MIGS.ba.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -75,3 +77,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.human-associated.6.0_template.csv
index 75e4408..d36a94a 100644
--- a/shiny/templates/config.biosample.MIGS.ba.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -79,3 +81,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.human-gut.6.0_template.csv
index ab5ef1a..3dacb3e 100644
--- a/shiny/templates/config.biosample.MIGS.ba.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -63,3 +65,5 @@ bs-special_diet,Optional,specification of special diet; can include multiple spe
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.human-oral.6.0_template.csv
index 88933e5..daaf6b6 100644
--- a/shiny/templates/config.biosample.MIGS.ba.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -62,3 +64,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.human-skin.6.0_template.csv
index 33c2ae6..351d5c9 100644
--- a/shiny/templates/config.biosample.MIGS.ba.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -63,3 +65,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.human-vaginal.6.0_template.csv
index 31b35e8..9066e12 100644
--- a/shiny/templates/config.biosample.MIGS.ba.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -70,3 +72,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-cores.6.0_template.csv
index 1f1242f..da4935d 100644
--- a/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
@@ -109,3 +111,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-fluids.swabs.6.0_template.csv
index 2b704d8..04256a7 100644
--- a/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
@@ -113,3 +115,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.microbial.6.0_template.csv
index c7e5407..f111ccc 100644
--- a/shiny/templates/config.biosample.MIGS.ba.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -92,3 +94,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.miscellaneous.6.0_template.csv
index 0d66ef6..65d572b 100644
--- a/shiny/templates/config.biosample.MIGS.ba.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -73,3 +75,5 @@ bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.plant-associated.6.0_template.csv
index f61f186..687abe2 100644
--- a/shiny/templates/config.biosample.MIGS.ba.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -101,3 +103,5 @@ bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth medi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.sediment.6.0_template.csv
index 1bda24b..78ff93d 100644
--- a/shiny/templates/config.biosample.MIGS.ba.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -98,3 +100,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.soil.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.soil.6.0_template.csv
index 478e50b..e1435ff 100644
--- a/shiny/templates/config.biosample.MIGS.ba.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -84,3 +86,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.symbiont-associated.6.0_template.csv
index 6cdfd52..bac332f 100644
--- a/shiny/templates/config.biosample.MIGS.ba.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -91,3 +93,5 @@ bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbio
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.wastewater.6.0_template.csv
index 955e135..dd1c4e4 100644
--- a/shiny/templates/config.biosample.MIGS.ba.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -67,3 +69,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.ba.water.6.0_template.csv b/shiny/templates/config.biosample.MIGS.ba.water.6.0_template.csv
index 5025ca2..c27dc53 100644
--- a/shiny/templates/config.biosample.MIGS.ba.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.ba.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,Required,microbial or eukaryotic strain name
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -113,3 +115,5 @@ bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phos
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.6.0_template.csv
index a176213..ff09a44 100644
--- a/shiny/templates/config.biosample.MIGS.eu.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -33,3 +35,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.agriculture.6.0_template.csv
index e32d22d..5a88966 100644
--- a/shiny/templates/config.biosample.MIGS.eu.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -155,3 +157,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.air.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.air.6.0_template.csv
index 5c766c4..52bdd69 100644
--- a/shiny/templates/config.biosample.MIGS.eu.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -59,3 +61,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.built.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.built.6.0_template.csv
index e753cbe..aa52940 100644
--- a/shiny/templates/config.biosample.MIGS.eu.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -193,3 +195,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.food-animal.6.0_template.csv
index c020621..bb511c2 100644
--- a/shiny/templates/config.biosample.MIGS.eu.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -121,3 +123,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.food-farm.env.6.0_template.csv
index 8529d19..0b420ce 100644
--- a/shiny/templates/config.biosample.MIGS.eu.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -159,3 +161,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.food-human.foods.6.0_template.csv
index 739d8df..c2226ab 100644
--- a/shiny/templates/config.biosample.MIGS.eu.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -127,3 +129,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.food-prod.facility.6.0_template.csv
index 7312c45..33e32c7 100644
--- a/shiny/templates/config.biosample.MIGS.eu.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -117,3 +119,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.host-associated.6.0_template.csv
index 78e924e..d681346 100644
--- a/shiny/templates/config.biosample.MIGS.eu.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -78,3 +80,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.human-associated.6.0_template.csv
index cf1e8cf..c027cbf 100644
--- a/shiny/templates/config.biosample.MIGS.eu.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -82,3 +84,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.human-gut.6.0_template.csv
index 5cb0c7e..356eaa9 100644
--- a/shiny/templates/config.biosample.MIGS.eu.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -66,3 +68,5 @@ bs-special_diet,Optional,specification of special diet; can include multiple spe
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.human-oral.6.0_template.csv
index 10a8aac..994f939 100644
--- a/shiny/templates/config.biosample.MIGS.eu.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -65,3 +67,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.human-skin.6.0_template.csv
index 2356c4d..e1c21d6 100644
--- a/shiny/templates/config.biosample.MIGS.eu.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -66,3 +68,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.human-vaginal.6.0_template.csv
index 1ca523b..6e6f19f 100644
--- a/shiny/templates/config.biosample.MIGS.eu.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -73,3 +75,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-cores.6.0_template.csv
index 157cdb5..f353a28 100644
--- a/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -112,3 +114,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-fluids.swabs.6.0_template.csv
index 82858b7..d6435eb 100644
--- a/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -116,3 +118,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.microbial.6.0_template.csv
index 8e5a8a7..fadb691 100644
--- a/shiny/templates/config.biosample.MIGS.eu.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -95,3 +97,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.miscellaneous.6.0_template.csv
index 84b94d4..a4e9133 100644
--- a/shiny/templates/config.biosample.MIGS.eu.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -76,3 +78,5 @@ bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.plant-associated.6.0_template.csv
index a1139f2..769cb9e 100644
--- a/shiny/templates/config.biosample.MIGS.eu.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -104,3 +106,5 @@ bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth medi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.sediment.6.0_template.csv
index 6237b03..4579aaf 100644
--- a/shiny/templates/config.biosample.MIGS.eu.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -101,3 +103,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.soil.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.soil.6.0_template.csv
index a7546b9..32617a9 100644
--- a/shiny/templates/config.biosample.MIGS.eu.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -87,3 +89,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.symbiont-associated.6.0_template.csv
index 398875c..56a61f4 100644
--- a/shiny/templates/config.biosample.MIGS.eu.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -94,3 +96,5 @@ bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbio
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.wastewater.6.0_template.csv
index 3373f96..00b1da8 100644
--- a/shiny/templates/config.biosample.MIGS.eu.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -70,3 +72,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.eu.water.6.0_template.csv b/shiny/templates/config.biosample.MIGS.eu.water.6.0_template.csv
index fc8ceaa..1ce3cbc 100644
--- a/shiny/templates/config.biosample.MIGS.eu.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.eu.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -116,3 +118,5 @@ bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phos
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.6.0_template.csv
index b59efe0..3eb0ba9 100644
--- a/shiny/templates/config.biosample.MIGS.vi.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -29,3 +31,5 @@ bs-samp_size,Optional,"Amount or size of sample (volume, mass or area) that was
bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.agriculture.6.0_template.csv
index cd5129a..39fe9e5 100644
--- a/shiny/templates/config.biosample.MIGS.vi.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
@@ -152,3 +154,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.air.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.air.6.0_template.csv
index 89fc133..8b9a8bc 100644
--- a/shiny/templates/config.biosample.MIGS.vi.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
@@ -55,3 +57,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.built.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.built.6.0_template.csv
index 3ea47a5..0019efb 100644
--- a/shiny/templates/config.biosample.MIGS.vi.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
@@ -189,3 +191,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.food-animal.6.0_template.csv
index f7d0bbd..8cbb041 100644
--- a/shiny/templates/config.biosample.MIGS.vi.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
@@ -117,3 +119,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.food-farm.env.6.0_template.csv
index 3cef84e..7333a60 100644
--- a/shiny/templates/config.biosample.MIGS.vi.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
@@ -155,3 +157,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.food-human.foods.6.0_template.csv
index 9fed675..9ada9f1 100644
--- a/shiny/templates/config.biosample.MIGS.vi.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
@@ -123,3 +125,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.food-prod.facility.6.0_template.csv
index 55b2eeb..edee162 100644
--- a/shiny/templates/config.biosample.MIGS.vi.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
@@ -113,3 +115,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.host-associated.6.0_template.csv
index add30cc..28888cf 100644
--- a/shiny/templates/config.biosample.MIGS.vi.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -74,3 +76,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.human-associated.6.0_template.csv
index 52cef7a..263a465 100644
--- a/shiny/templates/config.biosample.MIGS.vi.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -78,3 +80,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.human-gut.6.0_template.csv
index e873681..fb6038c 100644
--- a/shiny/templates/config.biosample.MIGS.vi.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -62,3 +64,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.human-oral.6.0_template.csv
index b358693..604ab43 100644
--- a/shiny/templates/config.biosample.MIGS.vi.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -61,3 +63,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.human-skin.6.0_template.csv
index 7670dcb..66e4eae 100644
--- a/shiny/templates/config.biosample.MIGS.vi.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -62,3 +64,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.human-vaginal.6.0_template.csv
index d8924e9..0c58592 100644
--- a/shiny/templates/config.biosample.MIGS.vi.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -69,3 +71,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-cores.6.0_template.csv
index 02be6c3..705b25d 100644
--- a/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
@@ -108,3 +110,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-fluids.swabs.6.0_template.csv
index f717f0e..b7ea3b2 100644
--- a/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
@@ -112,3 +114,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.microbial.6.0_template.csv
index 74b17c7..899747a 100644
--- a/shiny/templates/config.biosample.MIGS.vi.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -91,3 +93,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.miscellaneous.6.0_template.csv
index 15d9203..155c9b0 100644
--- a/shiny/templates/config.biosample.MIGS.vi.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -72,3 +74,5 @@ bs-sulfate,Optional,concentration of sulfate
bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.plant-associated.6.0_template.csv
index d97e4fe..7c2d05f 100644
--- a/shiny/templates/config.biosample.MIGS.vi.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -100,3 +102,5 @@ bs-temp,Optional,temperature of the sample at time of sampling
bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.sediment.6.0_template.csv
index 7a9e20d..b0c2a31 100644
--- a/shiny/templates/config.biosample.MIGS.vi.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -97,3 +99,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.soil.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.soil.6.0_template.csv
index 35797ee..cae3f7e 100644
--- a/shiny/templates/config.biosample.MIGS.vi.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -83,3 +85,5 @@ bs-tot_org_c_meth,Optional,reference or method used in determining total organic
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.symbiont-associated.6.0_template.csv
index 706dbb5..e72f7d8 100644
--- a/shiny/templates/config.biosample.MIGS.vi.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -90,3 +92,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host"
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.wastewater.6.0_template.csv
index a3b0175..e582564 100644
--- a/shiny/templates/config.biosample.MIGS.vi.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -66,3 +68,5 @@ bs-tertiary_treatment,Optional,the process providing a final treatment stage to
bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIGS.vi.water.6.0_template.csv b/shiny/templates/config.biosample.MIGS.vi.water.6.0_template.csv
index 24a6ef0..2eb540f 100644
--- a/shiny/templates/config.biosample.MIGS.vi.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIGS.vi.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -112,3 +114,5 @@ bs-tot_part_carb,Optional,total particulate carbon content
bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.6.0_template.csv
index 6a43700..0f8eb32 100644
--- a/shiny/templates/config.biosample.MIMAG.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -22,3 +24,5 @@ bs-samp_size,Optional,"Amount or size of sample (volume, mass or area) that was
bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.agriculture.6.0_template.csv
index 70bb96a..eda0ea2 100644
--- a/shiny/templates/config.biosample.MIMAG.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -151,3 +153,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.air.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.air.6.0_template.csv
index 07ac42a..7c0bad4 100644
--- a/shiny/templates/config.biosample.MIMAG.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -48,3 +50,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.built.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.built.6.0_template.csv
index 062fa79..1471b14 100644
--- a/shiny/templates/config.biosample.MIMAG.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
bs-air_temp,Required,temperature of the air at the time of sampling
@@ -182,3 +184,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.food-animal.6.0_template.csv
index ddfc489..aa96575 100644
--- a/shiny/templates/config.biosample.MIMAG.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -109,3 +111,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.food-farm.env.6.0_template.csv
index e7c67c9..d093ff0 100644
--- a/shiny/templates/config.biosample.MIMAG.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
@@ -150,3 +152,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.food-human.foods.6.0_template.csv
index 9821e4b..0a0a9db 100644
--- a/shiny/templates/config.biosample.MIMAG.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -115,3 +117,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.food-prod.facility.6.0_template.csv
index 81ba579..1e08c8e 100644
--- a/shiny/templates/config.biosample.MIMAG.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -106,3 +108,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.host-associated.6.0_template.csv
index 4662c43..8faf8ed 100644
--- a/shiny/templates/config.biosample.MIMAG.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -69,3 +71,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.human-associated.6.0_template.csv
index 88176d9..5f64382 100644
--- a/shiny/templates/config.biosample.MIMAG.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -72,3 +74,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.human-gut.6.0_template.csv
index 78ae91b..5fdf831 100644
--- a/shiny/templates/config.biosample.MIMAG.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -56,3 +58,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.human-oral.6.0_template.csv
index f4b1a4b..0780228 100644
--- a/shiny/templates/config.biosample.MIMAG.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -55,3 +57,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.human-skin.6.0_template.csv
index 282c789..8f22f1f 100644
--- a/shiny/templates/config.biosample.MIMAG.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -56,3 +58,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.human-vaginal.6.0_template.csv
index 877e2fb..4a46379 100644
--- a/shiny/templates/config.biosample.MIMAG.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -63,3 +65,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.hydrocarbon-cores.6.0_template.csv
index b11ff18..72b194a 100644
--- a/shiny/templates/config.biosample.MIMAG.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
@@ -101,3 +103,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.hydrocarbon-fluids.swabs.6.0_template.csv
index 0a4c8f5..07c7f86 100644
--- a/shiny/templates/config.biosample.MIMAG.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
@@ -105,3 +107,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.microbial.6.0_template.csv
index 014bf8a..88ca20f 100644
--- a/shiny/templates/config.biosample.MIMAG.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -84,3 +86,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.miscellaneous.6.0_template.csv
index e20ca09..4edc226 100644
--- a/shiny/templates/config.biosample.MIMAG.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -65,3 +67,5 @@ bs-sulfate,Optional,concentration of sulfate
bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.plant-associated.6.0_template.csv
index 9b20ee9..132a5f9 100644
--- a/shiny/templates/config.biosample.MIMAG.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -95,3 +97,5 @@ bs-temp,Optional,temperature of the sample at time of sampling
bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.sediment.6.0_template.csv
index 9daa4a6..381f125 100644
--- a/shiny/templates/config.biosample.MIMAG.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -90,3 +92,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.soil.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.soil.6.0_template.csv
index 450811e..4d1e1b4 100644
--- a/shiny/templates/config.biosample.MIMAG.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -76,3 +78,5 @@ bs-tot_org_c_meth,Optional,reference or method used in determining total organic
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.symbiont-associated.6.0_template.csv
index 3c3f689..99cfb79 100644
--- a/shiny/templates/config.biosample.MIMAG.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -84,3 +86,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host"
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.wastewater.6.0_template.csv
index 4652c7e..cfb74cb 100644
--- a/shiny/templates/config.biosample.MIMAG.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -59,3 +61,5 @@ bs-tertiary_treatment,Optional,the process providing a final treatment stage to
bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMAG.water.6.0_template.csv b/shiny/templates/config.biosample.MIMAG.water.6.0_template.csv
index e32e9bb..d679ce4 100644
--- a/shiny/templates/config.biosample.MIMAG.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMAG.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -105,3 +107,5 @@ bs-tot_part_carb,Optional,total particulate carbon content
bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.6.0_template.csv
index 5e4ffae..294a663 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -25,3 +27,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.agriculture.6.0_template.csv
index d702397..6b5a03c 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -151,3 +153,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.air.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.air.6.0_template.csv
index 3f81e64..26357bf 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -51,3 +53,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.built.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.built.6.0_template.csv
index 00275a3..07edde8 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -185,3 +187,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.food-animal.6.0_template.csv
index 5db91f3..f5c4099 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -113,3 +115,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.food-farm.env.6.0_template.csv
index 6777ff9..9c1a3b5 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -153,3 +155,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.food-human.foods.6.0_template.csv
index 385a2bf..a544267 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -119,3 +121,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.food-prod.facility.6.0_template.csv
index e55d6cb..90db43b 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -109,3 +111,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.host-associated.6.0_template.csv
index 1a4b638..8076861 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -73,3 +75,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.human-associated.6.0_template.csv
index 9e872d0..1ce3436 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -76,3 +78,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.human-gut.6.0_template.csv
index 3dd3c78..8109ad1 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -60,3 +62,5 @@ bs-special_diet,Optional,specification of special diet; can include multiple spe
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.human-oral.6.0_template.csv
index 5512450..b8e1958 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -59,3 +61,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.human-skin.6.0_template.csv
index 8263923..7b8131f 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -60,3 +62,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.human-vaginal.6.0_template.csv
index 7489989..498c7da 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -67,3 +69,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-cores.6.0_template.csv
index 675c94a..f2f45f0 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -104,3 +106,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0_template.csv
index 0cb2be9..42875d3 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -108,3 +110,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.microbial.6.0_template.csv
index 7d11880..6b5781b 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -87,3 +89,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.miscellaneous.6.0_template.csv
index 28f18a5..aeb0211 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -68,3 +70,5 @@ bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.plant-associated.6.0_template.csv
index 209c79a..16e344d 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -99,3 +101,5 @@ bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth medi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.sediment.6.0_template.csv
index b18f281..7f3216a 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -93,3 +95,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.soil.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.soil.6.0_template.csv
index 4821c7f..924ff7f 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -79,3 +81,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.symbiont-associated.6.0_template.csv
index 40581c4..312e8b3 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -87,3 +89,5 @@ bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbio
bs-temp,Optional,temperature of the sample at time of sampling
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.wastewater.6.0_template.csv
index 6e3542b..44aa7c5 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -62,3 +64,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.specimen.water.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.specimen.water.6.0_template.csv
index f9a699b..d45c038 100644
--- a/shiny/templates/config.biosample.MIMARKS.specimen.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.specimen.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
@@ -108,3 +110,5 @@ bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phos
bs-trophic_level,Optional,"Feeding position in food chain (eg., chemolithotroph)"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.agriculture.6.0_template.csv
index d2e4dae..dc86447 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -146,3 +148,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.air.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.air.6.0_template.csv
index e72a1fc..28146fc 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -43,3 +45,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.built.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.built.6.0_template.csv
index 2512820..2a18551 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
bs-air_temp,Required,temperature of the air at the time of sampling
bs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used
@@ -177,3 +179,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.food-animal.6.0_template.csv
index bfd768e..fe376bf 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -105,3 +107,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.food-farm.env.6.0_template.csv
index 28c89ed..cad740e 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -145,3 +147,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.food-human.foods.6.0_template.csv
index 581f13e..eb719cb 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -111,3 +113,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.food-prod.facility.6.0_template.csv
index 9fc85a1..0a416d9 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -102,3 +104,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.host-associated.6.0_template.csv
index eb52260..619b0fb 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -65,3 +67,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.human-associated.6.0_template.csv
index cf8c6bd..748908f 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -68,3 +70,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.human-gut.6.0_template.csv
index 382b30f..951b6a2 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -52,3 +54,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.human-oral.6.0_template.csv
index 58542f1..b6afcc6 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -51,3 +53,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.human-skin.6.0_template.csv
index 9226b0c..949f634 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -52,3 +54,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.human-vaginal.6.0_template.csv
index 5749877..61418ce 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -59,3 +61,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-cores.6.0_template.csv
index 9599b61..15d3dbc 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -96,3 +98,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-fluids.swabs.6.0_template.csv
index 16fc27a..c95046d 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
@@ -100,3 +102,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.microbial.6.0_template.csv
index 07b88b0..8182b6b 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -79,3 +81,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.miscellaneous.6.0_template.csv
index 038c548..3a65a3d 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -60,3 +62,5 @@ bs-sulfate,Optional,concentration of sulfate
bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.plant-associated.6.0_template.csv
index cdc4aa7..52021b1 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -91,3 +93,5 @@ bs-temp,Optional,temperature of the sample at time of sampling
bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.sediment.6.0_template.csv
index af6d149..491f778 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -85,3 +87,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.soil.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.soil.6.0_template.csv
index cf7764f..870d884 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -71,3 +73,5 @@ bs-tot_org_c_meth,Optional,reference or method used in determining total organic
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.symbiont-associated.6.0_template.csv
index 509c7f9..072e6e8 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -79,3 +81,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host"
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.wastewater.6.0_template.csv
index 4b1589e..1ef939c 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -54,3 +56,5 @@ bs-tertiary_treatment,Optional,the process providing a final treatment stage to
bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMARKS.survey.water.6.0_template.csv b/shiny/templates/config.biosample.MIMARKS.survey.water.6.0_template.csv
index e83b039..2543d20 100644
--- a/shiny/templates/config.biosample.MIMARKS.survey.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMARKS.survey.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -100,3 +102,5 @@ bs-tot_part_carb,Optional,total particulate carbon content
bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.agriculture.6.0_template.csv
index 9d7b300..eab0174 100644
--- a/shiny/templates/config.biosample.MIMS.me.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -147,3 +149,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.air.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.air.6.0_template.csv
index ea3151b..0ac6d58 100644
--- a/shiny/templates/config.biosample.MIMS.me.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -44,3 +46,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.built.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.built.6.0_template.csv
index 671fe15..db5aed5 100644
--- a/shiny/templates/config.biosample.MIMS.me.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
bs-air_temp,Required,temperature of the air at the time of sampling
bs-build_occup_type,Required,primary function for which a building or discrete part of a building is intended to be used
@@ -178,3 +180,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.food-animal.6.0_template.csv
index d378ef6..22e8cec 100644
--- a/shiny/templates/config.biosample.MIMS.me.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -106,3 +108,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.food-farm.env.6.0_template.csv
index 18a5235..6c2946f 100644
--- a/shiny/templates/config.biosample.MIMS.me.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
@@ -146,3 +148,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.food-human.foods.6.0_template.csv
index 81da9c2..3122fc7 100644
--- a/shiny/templates/config.biosample.MIMS.me.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -112,3 +114,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.food-prod.facility.6.0_template.csv
index 05e084a..07f4310 100644
--- a/shiny/templates/config.biosample.MIMS.me.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -103,3 +105,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.host-associated.6.0_template.csv
index 0df693e..82c8df7 100644
--- a/shiny/templates/config.biosample.MIMS.me.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -66,3 +68,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.human-associated.6.0_template.csv
index 796a303..69aa0a2 100644
--- a/shiny/templates/config.biosample.MIMS.me.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -69,3 +71,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.human-gut.6.0_template.csv
index 90f73d7..70344bf 100644
--- a/shiny/templates/config.biosample.MIMS.me.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -53,3 +55,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.human-oral.6.0_template.csv
index 36ac4b0..8ca0357 100644
--- a/shiny/templates/config.biosample.MIMS.me.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -52,3 +54,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.human-skin.6.0_template.csv
index ded011f..c0c78a1 100644
--- a/shiny/templates/config.biosample.MIMS.me.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -53,3 +55,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.human-vaginal.6.0_template.csv
index ad3937b..21205a3 100644
--- a/shiny/templates/config.biosample.MIMS.me.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -60,3 +62,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.hydrocarbon-cores.6.0_template.csv
index 66d4c33..2392005 100644
--- a/shiny/templates/config.biosample.MIMS.me.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -97,3 +99,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.hydrocarbon-fluids.swabs.6.0_template.csv
index 7185f42..18e9600 100644
--- a/shiny/templates/config.biosample.MIMS.me.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
@@ -101,3 +103,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.microbial.6.0_template.csv
index 83ef9f6..92a76bc 100644
--- a/shiny/templates/config.biosample.MIMS.me.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -80,3 +82,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.miscellaneous.6.0_template.csv
index 740b154..9e23fa6 100644
--- a/shiny/templates/config.biosample.MIMS.me.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -61,3 +63,5 @@ bs-sulfate,Optional,concentration of sulfate
bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.plant-associated.6.0_template.csv
index fd69619..616fd0c 100644
--- a/shiny/templates/config.biosample.MIMS.me.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -92,3 +94,5 @@ bs-temp,Optional,temperature of the sample at time of sampling
bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.sediment.6.0_template.csv
index 0b430de..782e4dc 100644
--- a/shiny/templates/config.biosample.MIMS.me.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -86,3 +88,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.soil.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.soil.6.0_template.csv
index def152a..2c1ecfd 100644
--- a/shiny/templates/config.biosample.MIMS.me.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -72,3 +74,5 @@ bs-tot_org_c_meth,Optional,reference or method used in determining total organic
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.symbiont-associated.6.0_template.csv
index e8e0f2f..0b01531 100644
--- a/shiny/templates/config.biosample.MIMS.me.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -80,3 +82,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host"
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.wastewater.6.0_template.csv
index 02e9154..0077370 100644
--- a/shiny/templates/config.biosample.MIMS.me.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
bs-env_medium,Required,Add terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material [ENVO:00010483]. Multiple terms can be separated by pipes e.g.: estuarine water [ENVO:01000301]|estuarine mud [ENVO:00002160]
@@ -55,3 +57,5 @@ bs-tertiary_treatment,Optional,the process providing a final treatment stage to
bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIMS.me.water.6.0_template.csv b/shiny/templates/config.biosample.MIMS.me.water.6.0_template.csv
index fe140e3..d858c0d 100644
--- a/shiny/templates/config.biosample.MIMS.me.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIMS.me.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -101,3 +103,5 @@ bs-tot_part_carb,Optional,total particulate carbon content
bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.6.0_template.csv b/shiny/templates/config.biosample.MISAG.6.0_template.csv
index ea1373e..0a98a81 100644
--- a/shiny/templates/config.biosample.MISAG.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -21,3 +23,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MISAG.agriculture.6.0_template.csv
index 51b6836..b170f80 100644
--- a/shiny/templates/config.biosample.MISAG.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -150,3 +152,5 @@ bs-water_source_shared,Optional,"Other users sharing access to the same water so
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.air.6.0_template.csv b/shiny/templates/config.biosample.MISAG.air.6.0_template.csv
index 5923e0e..3f20439 100644
--- a/shiny/templates/config.biosample.MISAG.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-altitude,Required,The altitude of the sample is the vertical distance between Earth's surface above Sea Level and the sampled position in the air.
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -47,3 +49,5 @@ bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easil
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.built.6.0_template.csv b/shiny/templates/config.biosample.MISAG.built.6.0_template.csv
index a4de34d..9517f25 100644
--- a/shiny/templates/config.biosample.MISAG.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-abs_air_humidity,Required,actual mass of water vapor - mh20 - present in the air water vapor mixture
bs-air_temp,Required,temperature of the air at the time of sampling
@@ -181,3 +183,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MISAG.food-animal.6.0_template.csv
index 7da6759..e68fd26 100644
--- a/shiny/templates/config.biosample.MISAG.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -108,3 +110,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MISAG.food-farm.env.6.0_template.csv
index 9e6a7f4..af1d25b 100644
--- a/shiny/templates/config.biosample.MISAG.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-biotic_regm,Required,"Information about treatment(s) involving use of biotic factors, such as bacteria, viruses or fungi"
bs-chem_administration,Required,"list of chemical compounds administered to the host or site where sampling occurred, and when (e.g. antibiotics, N fertilizer, air filter); can include multiple compounds. For Chemical Entities of Biological Interest ontology (CHEBI) (v1.72), please see http://bioportal.bioontology.org/visualize/44603"
@@ -149,3 +151,5 @@ bs-water_source_shared,Optional,"Other users sharing access to the same water so
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MISAG.food-human.foods.6.0_template.csv
index e5c7aad..5789ef2 100644
--- a/shiny/templates/config.biosample.MISAG.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -114,3 +116,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MISAG.food-prod.facility.6.0_template.csv
index 66752a7..b7177e8 100644
--- a/shiny/templates/config.biosample.MISAG.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-coll_site_geo_feat,Required,"Text or terms that describe the geographic feature where the food sample was obtained by the researcher. This field encourages selected terms listed under the following ontologies: anthropogenic geographic feature (http://purl.obolibrary.org/obo/ENVO_00000002), for example agricultural fairground [ENVO:01000986]; garden [ENVO:00000011} or any of its subclasses; market [ENVO:01000987]; water well [ENVO:01000002]; or human construction (http://purl.obolibrary.org/obo/ENVO_00000070), e.g., grocery store [GENEPIO:0001020]"
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -105,3 +107,5 @@ bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MISAG.host-associated.6.0_template.csv
index eea6d41..facbb79 100644
--- a/shiny/templates/config.biosample.MISAG.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -68,3 +70,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MISAG.human-associated.6.0_template.csv
index c3d983a..4d2617e 100644
--- a/shiny/templates/config.biosample.MISAG.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -71,3 +73,5 @@ bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MISAG.human-gut.6.0_template.csv
index 48bc3e9..c8c2c0c 100644
--- a/shiny/templates/config.biosample.MISAG.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -55,3 +57,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-temp,Optional,temperature of the sample at time of sampling
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MISAG.human-oral.6.0_template.csv
index 181fdfe..5dcba4f 100644
--- a/shiny/templates/config.biosample.MISAG.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -54,3 +56,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MISAG.human-skin.6.0_template.csv
index 1a59495..f869477 100644
--- a/shiny/templates/config.biosample.MISAG.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -55,3 +57,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MISAG.human-vaginal.6.0_template.csv
index 63106d7..b4ca9e4 100644
--- a/shiny/templates/config.biosample.MISAG.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -62,3 +64,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MISAG.hydrocarbon-cores.6.0_template.csv
index 42c20e8..ebf2e6b 100644
--- a/shiny/templates/config.biosample.MISAG.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
bs-basin_name,Required,"Name of the basin, e.g. Campos"
@@ -100,3 +102,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MISAG.hydrocarbon-fluids.swabs.6.0_template.csv
index 5759fa7..1fd9b77 100644
--- a/shiny/templates/config.biosample.MISAG.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-add_recov_method,Required,"Additional (i.e. secondary, tertiary, etc.) recovery methods deployed for increase of hydrocarbon recovery from resource and start date for each one of them, e.g., water injection {timestamp}, dump flood {timestamp}, gas injection {timestamp}, wag immiscible injection {timestamp}, polymer addition {timestamp}, surfactant addition {timestamp}"
bs-api,Required,"API gravity is a measure of how heavy or light a petroleum liquid is compared to water (source: https://en.wikipedia.org/wiki/API_gravity), e.g. 31.1 API"
@@ -104,3 +106,5 @@ bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.microbial.6.0_template.csv b/shiny/templates/config.biosample.MISAG.microbial.6.0_template.csv
index 7eaaebc..25f0a1d 100644
--- a/shiny/templates/config.biosample.MISAG.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -83,3 +85,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MISAG.miscellaneous.6.0_template.csv
index 0a6935d..361531f 100644
--- a/shiny/templates/config.biosample.MISAG.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -64,3 +66,5 @@ bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MISAG.plant-associated.6.0_template.csv
index 5d51d83..601fc1f 100644
--- a/shiny/templates/config.biosample.MISAG.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -94,3 +96,5 @@ bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth medi
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.sediment.6.0_template.csv b/shiny/templates/config.biosample.MISAG.sediment.6.0_template.csv
index 2ed0165..ede510a 100644
--- a/shiny/templates/config.biosample.MISAG.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -89,3 +91,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.soil.6.0_template.csv b/shiny/templates/config.biosample.MISAG.soil.6.0_template.csv
index 92f113b..c1a10ed 100644
--- a/shiny/templates/config.biosample.MISAG.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-elev,Required,The elevation of the sampling site as measured by the vertical distance from mean sea level.
@@ -75,3 +77,5 @@ bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soi
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MISAG.symbiont-associated.6.0_template.csv
index ad76865..ecd3277 100644
--- a/shiny/templates/config.biosample.MISAG.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -83,3 +85,5 @@ bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbio
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MISAG.wastewater.6.0_template.csv
index 7e1da72..e6eb526 100644
--- a/shiny/templates/config.biosample.MISAG.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
bs-env_local_scale,Required,"Add terms that identify environmental entities having causal influences upon the entity at time of sampling, multiple terms can be separated by pipes, e.g.: shoreline [ENVO:00000486]|intertidal zone [ENVO:00000316]"
@@ -58,3 +60,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MISAG.water.6.0_template.csv b/shiny/templates/config.biosample.MISAG.water.6.0_template.csv
index 763359e..c362b7c 100644
--- a/shiny/templates/config.biosample.MISAG.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MISAG.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-depth,Required,"Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectivly. Depth can be reported as an interval for subsurface samples."
bs-env_broad_scale,Required,Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes e.g.: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
@@ -104,3 +106,5 @@ bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phos
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
bs-wga_amp_appr,Optional,"method used to amplify genomic DNA in preparation for sequencing. Examples: MDR, WGA-X, MDA"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.6.0_template.csv
index b80c4ec..420d8b6 100644
--- a/shiny/templates/config.biosample.MIUVIG.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -27,3 +29,5 @@ bs-samp_size,Optional,"Amount or size of sample (volume, mass or area) that was
bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed for DNA extraction
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.agriculture.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.agriculture.6.0_template.csv
index 00ab623..4933fc5 100644
--- a/shiny/templates/config.biosample.MIUVIG.agriculture.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.agriculture.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -151,3 +153,5 @@ bs-water_ph,Optional,"The pH measurement of the sample, or liquid portion of sam
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.air.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.air.6.0_template.csv
index 5fd75d1..28ed84a 100644
--- a/shiny/templates/config.biosample.MIUVIG.air.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.air.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -53,3 +55,5 @@ bs-ventilation_type,Optional,ventilation system used in the sampled premises
bs-volatile_org_comp,Optional,concentration of carbon-based chemicals that easily evaporate at room temperature; can report multiple volatile organic compounds by entering numeric values preceded by name of compound
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.built.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.built.6.0_template.csv
index 0f0dce5..b080919 100644
--- a/shiny/templates/config.biosample.MIUVIG.built.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.built.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -187,3 +189,5 @@ bs-window_status,Optional,"Defines whether the windows were open or closed durin
bs-window_type,Optional,"The type of windows, e.g., single-hung sash window, horizontal sash window, fixed window"
bs-window_vert_pos,Optional,"The vertical position of the window on the wall, e.g., bottom, middle, top, low, middle, high"
bs-window_water_mold,Optional,"Signs of the presence of mold or mildew on the window, e.g., presence of mold visible, no presence of mold visible"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.food-animal.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.food-animal.6.0_template.csv
index 388ee6e..c0cb26c 100644
--- a/shiny/templates/config.biosample.MIUVIG.food-animal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.food-animal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -114,3 +116,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.food-farm.env.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.food-farm.env.6.0_template.csv
index 90b114b..57d17b5 100644
--- a/shiny/templates/config.biosample.MIUVIG.food-farm.env.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.food-farm.env.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -153,3 +155,5 @@ bs-water_source_adjac,Optional,Description of the environmental features that ar
bs-water_source_shared,Optional,"Other users sharing access to the same water source, e.g., multiple users, agricutural, multiple users, no sharing"
bs-wind_direction,Optional,wind direction is the direction from which a wind originates
bs-wind_speed,Optional,speed of wind measured at the time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.food-human.foods.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.food-human.foods.6.0_template.csv
index bb63c9a..2bcea93 100644
--- a/shiny/templates/config.biosample.MIUVIG.food-human.foods.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.food-human.foods.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -120,3 +122,5 @@ bs-study_timecourse,Optional,"For time-course research studies involving samples
bs-study_tmnt,Optional,"A process in which the act is intended to modify or alter some other material entity. From the study design, each treatment is comprised of one level of one or multiple factors. This field accepts terms listed under treatment (http://purl.obolibrary.org/obo/MCO_0000866). If the proper descriptor is not listed please use text to describe the study treatment. Multiple terms can be separated by one or more pipes"
bs-temp,Optional,temperature of the sample at time of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.food-prod.facility.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.food-prod.facility.6.0_template.csv
index ee7d57b..609b6cb 100644
--- a/shiny/templates/config.biosample.MIUVIG.food-prod.facility.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.food-prod.facility.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -111,3 +113,5 @@ bs-study_tmnt,Optional,"A process in which the act is intended to modify or alte
bs-subspecf_gen_lin,Optional,"Information about the genetic distinctness of the lineage (eg., biovar, serovar)"
bs-surf_material,Optional,surface materials at the point of sampling
bs-timepoint,Optional,Time point at which a sample or observation is made or taken from a biomaterial as measured from some reference point. Indicate the timepoint written in ISO 8601 format
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.host-associated.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.host-associated.6.0_template.csv
index def3e7e..efe6ab6 100644
--- a/shiny/templates/config.biosample.MIUVIG.host-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.host-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -72,3 +74,5 @@ bs-samp_vol_we_dna_ext,Optional,volume (mL) or weight (g) of sample processed fo
bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0.22 micrometer"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.human-associated.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.human-associated.6.0_template.csv
index 0c4ae99..4875808 100644
--- a/shiny/templates/config.biosample.MIUVIG.human-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.human-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -76,3 +78,5 @@ bs-twin_sibling,Optional,specification of twin sibling presence
bs-urine_collect_meth,Optional,specification of urine collection method
bs-urogenit_tract_disor,Optional,history of urogenitaltract disorders; can include multiple disorders
bs-weight_loss_3_month,Optional,"specification of weight loss in the last three months, if yes should be further specified to include amount of weight loss"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.human-gut.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.human-gut.6.0_template.csv
index a1d90b3..5fb3b2b 100644
--- a/shiny/templates/config.biosample.MIUVIG.human-gut.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.human-gut.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -60,3 +62,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-special_diet,Optional,specification of special diet; can include multiple special diets
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.human-oral.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.human-oral.6.0_template.csv
index 51dc086..360fba0 100644
--- a/shiny/templates/config.biosample.MIUVIG.human-oral.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.human-oral.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -59,3 +61,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_last_toothbrush,Optional,specification of the time since last toothbrushing
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.human-skin.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.human-skin.6.0_template.csv
index 0a068c4..8ff627a 100644
--- a/shiny/templates/config.biosample.MIUVIG.human-skin.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.human-skin.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -60,3 +62,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-time_since_last_wash,Optional,specification of the time since last wash
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.human-vaginal.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.human-vaginal.6.0_template.csv
index 3d6aea6..97cd6c0 100644
--- a/shiny/templates/config.biosample.MIUVIG.human-vaginal.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.human-vaginal.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -67,3 +69,5 @@ bs-size_frac,Optional,"Filtering pore size used in sample preparation, e.g., 0-0
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
bs-temp,Optional,temperature of the sample at time of sampling
bs-urogenit_disord,Optional,"history of urogenital disorders, can include multiple disorders"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.hydrocarbon-cores.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.hydrocarbon-cores.6.0_template.csv
index 1930903..70fd464 100644
--- a/shiny/templates/config.biosample.MIUVIG.hydrocarbon-cores.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.hydrocarbon-cores.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -106,3 +108,5 @@ bs-vfa,Optional,Concentration of Volatile Fatty Acids in the sample
bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by shear stress or tensile stress (e.g., 3.5 cp; 100C)"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.hydrocarbon-fluids.swabs.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.hydrocarbon-fluids.swabs.6.0_template.csv
index 5b40523..01d83c1 100644
--- a/shiny/templates/config.biosample.MIUVIG.hydrocarbon-fluids.swabs.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.hydrocarbon-fluids.swabs.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -110,3 +112,5 @@ bs-viscosity,Optional,"A measure of oil's resistance to gradual deformation by s
bs-water_prod_rate,Optional,"Water production rates per well, e.g., 987 m3/day"
bs-win,Optional,A unique identifier of a well or wellbore. This is part of the Global Framework for Well Identification initiative which is compiled by the Professional Petroleum Data Management Association (PPDM) in an effort to improve well identification systems. (Supporting information: https://ppdm.org/ and http://dl.ppdm.org/dl/690)
bs-xylene,Optional,Concentration of xylene in the sample
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.microbial.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.microbial.6.0_template.csv
index 0c44d20..132a274 100644
--- a/shiny/templates/config.biosample.MIUVIG.microbial.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.microbial.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -89,3 +91,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.miscellaneous.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.miscellaneous.6.0_template.csv
index b43ebde..92b3142 100644
--- a/shiny/templates/config.biosample.MIUVIG.miscellaneous.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.miscellaneous.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -70,3 +72,5 @@ bs-sulfate,Optional,concentration of sulfate
bs-sulfide,Optional,concentration of sulfide
bs-temp,Optional,temperature of the sample at time of sampling
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.plant-associated.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.plant-associated.6.0_template.csv
index 51ab651..2064dab 100644
--- a/shiny/templates/config.biosample.MIUVIG.plant-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.plant-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -98,3 +100,5 @@ bs-temp,Optional,temperature of the sample at time of sampling
bs-tiss_cult_growth_med,Optional,description of plant tissue culture growth media used
bs-water_temp_regm,Optional,information about treatment involving an exposure to water with varying degree of temperature; can include multiple regimens
bs-watering_regm,Optional,information about treatment involving an exposure to watering frequencies; can include multiple regimens
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.sediment.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.sediment.6.0_template.csv
index 911a128..8e652a4 100644
--- a/shiny/templates/config.biosample.MIUVIG.sediment.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.sediment.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -95,3 +97,5 @@ bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-turbidity,Optional,turbidity measurement
bs-water_content,Optional,water content measurement
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.soil.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.soil.6.0_template.csv
index 753b8fa..3333943 100644
--- a/shiny/templates/config.biosample.MIUVIG.soil.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.soil.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -81,3 +83,5 @@ bs-tot_org_c_meth,Optional,reference or method used in determining total organic
bs-tot_org_carb,Optional,Definition for soil: total organic C content of the soil units of g C/kg soil. Definition otherwise: total organic carbon content
bs-water_content,Optional,water content measurement
bs-water_content_soil_meth,Optional,reference or method used in determining the water content of soil
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.symbiont-associated.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.symbiont-associated.6.0_template.csv
index 36e5fa5..8af7b03 100644
--- a/shiny/templates/config.biosample.MIUVIG.symbiont-associated.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.symbiont-associated.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -88,3 +90,5 @@ bs-source_material_id,Optional,"unique identifier assigned to a material sample
bs-symbiont_host_role,Optional,"Role of the host in the life cycle of the symbiotic organism, e.g., accidental, dead-end, definitive, intermediate, paratenic, reservoir, single host"
bs-temp,Optional,temperature of the sample at time of sampling
bs-type_of_symbiosis,Optional,"Type of biological interaction established between the symbiotic host organism being sampled and its respective host, e.g., commensalistic, mutualistic, parasitic"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.wastewater.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.wastewater.6.0_template.csv
index 4252d12..ed4bcc8 100644
--- a/shiny/templates/config.biosample.MIUVIG.wastewater.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.wastewater.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -64,3 +66,5 @@ bs-tertiary_treatment,Optional,the process providing a final treatment stage to
bs-tot_nitro,Optional,total nitrogen content of the sample
bs-tot_phosphate,Optional,total amount or concentration of phosphate
bs-wastewater_type,Optional,"the origin of wastewater such as human waste, rainfall, storm drains, etc."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.MIUVIG.water.6.0_template.csv b/shiny/templates/config.biosample.MIUVIG.water.6.0_template.csv
index 12a83fb..398256c 100644
--- a/shiny/templates/config.biosample.MIUVIG.water.6.0_template.csv
+++ b/shiny/templates/config.biosample.MIUVIG.water.6.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -110,3 +112,5 @@ bs-tot_part_carb,Optional,total particulate carbon content
bs-tot_phosp,Optional,"total phosphorus concentration, calculated by: total phosphorus = total dissolved phosphorus + particulate phosphorus. Can also be measured without filtering, reported as phosphorus"
bs-turbidity,Optional,turbidity measurement
bs-water_current,Optional,measurement of magnitude and direction of flow within a fluid
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Metagenome.environmental.1.0_template.csv b/shiny/templates/config.biosample.Metagenome.environmental.1.0_template.csv
index ddbc97e..44e4e48 100644
--- a/shiny/templates/config.biosample.Metagenome.environmental.1.0_template.csv
+++ b/shiny/templates/config.biosample.Metagenome.environmental.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-isolation_source,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
bs-geo_loc_name,Required,"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg ""Canada: Vancouver"" or ""Germany: halfway down Zugspitze, Alps"""
@@ -10,3 +12,5 @@ bs-samp_collect_device,Optional,Method or device employed for collecting sample
bs-samp_mat_process,Optional,Processing applied to the sample during or after isolation
bs-samp_size,Optional,"Amount or size of sample (volume, mass or area) that was collected"
bs-source_material_id,Optional,"unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Microbe.1.0_template.csv b/shiny/templates/config.biosample.Microbe.1.0_template.csv
index 43036fb..43e13a5 100644
--- a/shiny/templates/config.biosample.Microbe.1.0_template.csv
+++ b/shiny/templates/config.biosample.Microbe.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-host,"At least one field required. Group: At least one required: Group ""Source","At least one required: Group ""Source"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
@@ -24,3 +26,5 @@ bs-serotype,Optional,"Taxonomy below subspecies; a variety (in bacteria, fungi o
bs-serovar,Optional,"Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Same as serovar and serotype. Sometimes used as species identifier in bacteria with shaky taxonomy, e.g. Leptospira, serovar saopaolo S76607 (65357 in Entrez)."
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Model.organism.animal.1.0_template.csv b/shiny/templates/config.biosample.Model.organism.animal.1.0_template.csv
index 098e001..c56a28e 100644
--- a/shiny/templates/config.biosample.Model.organism.animal.1.0_template.csv
+++ b/shiny/templates/config.biosample.Model.organism.animal.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-breed,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". breed name - chiefly used in domesticated animals or plants"
@@ -34,3 +36,5 @@ bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format:
bs-store_cond,Optional,explain how and for how long the soil sample was stored before DNA extraction.
bs-stud_book_number,Optional,
bs-treatment,Optional,
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.OneHealthEnteric.1.0_template.csv b/shiny/templates/config.biosample.OneHealthEnteric.1.0_template.csv
index f45e30c..e4a3a29 100644
--- a/shiny/templates/config.biosample.OneHealthEnteric.1.0_template.csv
+++ b/shiny/templates/config.biosample.OneHealthEnteric.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-collected_by,Required,Name of persons or institute who collected the sample
bs-geo_loc_name,Required,"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg ""Canada: Vancouver"" or ""Germany: halfway down Zugspitze, Alps"""
bs-isolation_source,Required,"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
@@ -77,3 +79,5 @@ bs-surf_material,Optional,surface materials at the point of sampling
bs-surf_temp,Optional,temperature of the surface at the time of sampling
bs-surface_orientation,Optional,"Describe which surface of the object is swabbed. (e.g underside, top, corner), e.g., underside"
bs-upstream_intervention,Optional,"List any known health-related interventions (prophylactic, therapeutic, and/or metaphylactic) administered to the host, i.e. vaccinations, hormonal patches, antibiotics, injections in feed, etc., e.g., prophylactic/metaphylactic antibiotics"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Pathogen.cl.1.0_template.csv b/shiny/templates/config.biosample.Pathogen.cl.1.0_template.csv
index c49cc72..d4f5b6f 100644
--- a/shiny/templates/config.biosample.Pathogen.cl.1.0_template.csv
+++ b/shiny/templates/config.biosample.Pathogen.cl.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-collected_by,Required,Name of persons or institute who collected the sample
@@ -25,3 +27,5 @@ bs-serovar,Optional,"Taxonomy below subspecies; a variety (in bacteria, fungi or
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-subgroup,Optional,Taxonomy below subspecies; sometimes used in viruses to denote subgroups taken from a single isolate.
bs-subtype,Optional,"Used as classifier in viruses (e.g. HIV type 1, Group M, Subtype A)."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Pathogen.env.1.0_template.csv b/shiny/templates/config.biosample.Pathogen.env.1.0_template.csv
index 332c432..1979755 100644
--- a/shiny/templates/config.biosample.Pathogen.env.1.0_template.csv
+++ b/shiny/templates/config.biosample.Pathogen.env.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-strain,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". microbial or eukaryotic strain name"
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-collected_by,Required,Name of persons or institute who collected the sample
@@ -15,3 +17,5 @@ bs-serovar,Optional,"Taxonomy below subspecies; a variety (in bacteria, fungi or
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-subgroup,Optional,Taxonomy below subspecies; sometimes used in viruses to denote subgroups taken from a single isolate.
bs-subtype,Optional,"Used as classifier in viruses (e.g. HIV type 1, Group M, Subtype A)."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Plant.1.0_template.csv b/shiny/templates/config.biosample.Plant.1.0_template.csv
index f922826..a0a6b79 100644
--- a/shiny/templates/config.biosample.Plant.1.0_template.csv
+++ b/shiny/templates/config.biosample.Plant.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". identification or description of the specific individual from which this sample was obtained"
bs-cultivar,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". cultivar name - cultivated variety of plant"
bs-ecotype,"At least one field required. Group: At least one required: Group ""Organism","At least one required: Group ""Organism"". a population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat, e.g., Columbia"
@@ -26,3 +28,5 @@ bs-sex,Optional,physical sex of sampled organism
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-temp,Optional,temperature of the sample at time of sampling
bs-treatment,Optional,
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.SARS-CoV-2.cl.1.0_template.csv b/shiny/templates/config.biosample.SARS-CoV-2.cl.1.0_template.csv
index e23e33e..9b73c7a 100644
--- a/shiny/templates/config.biosample.SARS-CoV-2.cl.1.0_template.csv
+++ b/shiny/templates/config.biosample.SARS-CoV-2.cl.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-collected_by,Required,Name of persons or institute who collected the sample
bs-geo_loc_name,Required,"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg ""Canada: Vancouver"" or ""Germany: halfway down Zugspitze, Alps"""
bs-host,Required,"The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
@@ -42,3 +44,5 @@ bs-sars_cov_2_diag_pcr_ct_value_2,Optional,"The cycle threshold (CT) value resul
bs-sequenced_by,Optional,"The name of the agency that generated the sequence, e.g., Centers for Disease Control and Prevention"
bs-vaccine_received,Optional,"Which vaccine did the host receive, e.g., Pfizer-BioNTech COVID-19 vaccine"
bs-virus_isolate_of_prior_infection,Optional,"Specific isolate of SARS-CoV-2 in prior infection (if known), e.g., SARS-CoV-2/human/USA/CA-CDPH-001/2020"
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.SARS-CoV-2.wwsurv.1.0_template.csv b/shiny/templates/config.biosample.SARS-CoV-2.wwsurv.1.0_template.csv
index 3e2882e..d9bfaab 100644
--- a/shiny/templates/config.biosample.SARS-CoV-2.wwsurv.1.0_template.csv
+++ b/shiny/templates/config.biosample.SARS-CoV-2.wwsurv.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-geo_loc_name,Required,"Geographical origin of the sample; use the appropriate name from this list http://www.insdc.org/documents/country-qualifier-vocabulary. Use a colon to separate the country or ocean from more detailed information about the location, eg ""Canada: Vancouver"" or ""Germany: halfway down Zugspitze, Alps"""
bs-isolation_source,Required,"Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived."
bs-ww_population,Required,"Number of persons contributing wastewater to this sample collection site; if unknown, estimate to the nearest order of magnitude, e.g., 10000. If no estimate is available, input NA."
@@ -47,3 +49,5 @@ bs-ww_surv_target_2_known_present,Optional,"Is genetic material of the surveilla
bs-ww_surv_target_2_protocol,Optional,"The protocol used to quantify 'ww_surv_target_2'. Specify a reference, website, or brief description."
bs-ww_temperature,Optional,"Temperature of the wastewater sample at the time of sampling in Celsius, e.g., 25."
bs-ww_total_suspended_solids,Optional,"Total concentration of solids in raw wastewater influent sample including a wide variety of material, such as silt, decaying plant and animal matter in milligrams per liter, e.g., 500."
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.biosample.Virus.1.0_template.csv b/shiny/templates/config.biosample.Virus.1.0_template.csv
index c6c9d81..b37cb94 100644
--- a/shiny/templates/config.biosample.Virus.1.0_template.csv
+++ b/shiny/templates/config.biosample.Virus.1.0_template.csv
@@ -1,5 +1,7 @@
column_name,required_column,description
bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters.
+bs-sample_title,Optional,Optional additional title for sample. Will be autogenerated by NCBI if not provided.
+bs-sample_description,Optional,Optional description for sample.
bs-isolate,Required,identification or description of the specific individual from which this sample was obtained
bs-host,"At least one field required. Group: At least one required: Group ""Host","At least one required: Group ""Host"". The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name, eg, ""Homo sapiens""."
bs-lab_host,"At least one field required. Group: At least one required: Group ""Host","At least one required: Group ""Host"". Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained, e.g., Escherichia coli DH5a, or Homo sapiens HeLa cells"
@@ -22,3 +24,5 @@ bs-serotype,Optional,"Taxonomy below subspecies; a variety (in bacteria, fungi o
bs-specimen_voucher,Optional,"Identifier for the physical specimen. Use format: ""[:[:]]"", eg, ""UAM:Mamm:52179"". Intended as a reference to the physical specimen that remains after it was analyzed. If the specimen was destroyed in the process of analysis, electronic images (e-vouchers) are an adequate substitute for a physical voucher specimen. Ideally the specimens will be deposited in a curated museum, herbarium, or frozen tissue collection, but often they will remain in a personal or laboratory collection for some time before they are deposited in a curated collection. There are three forms of specimen_voucher qualifiers. If the text of the qualifier includes one or more colons it is a 'structured voucher'. Structured vouchers include institution-codes (and optional collection-codes) taken from a controlled vocabulary maintained by the INSDC that denotes the museum or herbarium collection where the specimen resides, please visit: http://www.insdc.org/controlled-vocabulary-specimenvoucher-qualifier."
bs-strain,Optional,microbial or eukaryotic strain name
bs-temp,Optional,temperature of the sample at time of sampling
+bs-title,Optional,"Optional internal field for how the BioSample submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-BS"" will be used instead."
+bs-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.genbank.genbank.flu.src.schema_template.csv b/shiny/templates/config.genbank.genbank.flu.src.schema_template.csv
new file mode 100644
index 0000000..24b2cdb
--- /dev/null
+++ b/shiny/templates/config.genbank.genbank.flu.src.schema_template.csv
@@ -0,0 +1,50 @@
+column_name,required_column,description
+src-Altitude,Optional,Altitude in metres above or below sea level of where the sample was collected.
+src-Authority,Optional,The author or authors of the organism name from which sequence was obtained.
+src-Bio_material,Optional,"An identifier for the biological material from which the nucleotide sequence was obtained, with optional institution code and collection code for the place where it is currently stored. This should be provided using the following format 'institution-code:collection-code:material_id'. material_id is mandatory, institution-code and collection-code are optional; institution-code is mandatory when collection-code is present. This qualifier should be used to annotate the identifiers of material in biological collections which include zoos and aquaria, stock centers, seed banks, germplasm repositories and DNA banks."
+src-Biotype,Optional,"Variety of a species (usually a fungus, bacteria, or virus) characterized by some specific biological property (often geographical, ecological, or physiological). Same as biotype."
+src-Biovar,Optional,See biotype
+src-Breed,Optional,The named breed from which sequence was obtained (usually applied to domesticated mammals).
+src-Cell_line,Optional,Cell line from which sequence was obtained.
+src-Cell_type,Optional,Type of cell from which sequence was obtained.
+src-Chemovar,Optional,"Variety of a species (usually a fungus, bacteria, or virus) characterized by its biochemical properties."
+src-Clone,Optional,Name of clone from which sequence was obtained.
+src-Collected_by,Optional,Name of person who collected the sample.
+src-Country,Optional,"The country where the sequence's organism was located. May also be an ocean or major sea. Additional region or locality information must be after the country name and separated by a ':'. For example: USA: Riverview Park, Ripkentown, MD"
+src-Cultivar,Optional,Cultivated variety of plant from which sequence was obtained.
+src-Culture_collection,Optional,"Institution code and identifier for the culture from which the nucleotide sequence was obtained, with optional collection code. This should be provided using the following format 'institution-code:collection-code:culture-id'. culture-id and institution-code are mandatory. This qualifier should be used to annotate live microbial and viral cultures, and cell lines that have been deposited in curated culture collections."
+src-Dev_stage,Optional,Developmental stage of organism.
+src-Ecotype,Optional,The named ecotype (population adapted to a local habitat) from which sequence was obtained (customarily applied to populations of Arabidopsis thaliana).
+src-Forma,Optional,The forma (lowest taxonomic unit governed by the nomenclatural codes) of organism from which sequence was obtained. This term is usually applied to plants and fungi.
+src-Forma_specialis,Optional,The physiologically distinct form from which sequence was obtained (usually restricted to certain parasitic fungi).
+src-Fwd_primer_name,Optional,name of forward PCR primer
+src-Fwd_primer_seq,Optional,nucleotide sequence of forward PCR primer
+src-Genotype,Optional,Genotype of the organism.
+src-Haplogroup,Optional,Name for a group of similar haplotypes that share some sequence variation
+src-Haplotype,Optional,Haplotype of the organism.
+src-Host,Optional,"When the sequence submission is from an organism that exists in a symbiotic, parasitic, or other special relationship with some second organism, the 'host' modifier can be used to identify the name of the host species."
+src-Identified_by,Optional,name of the person or persons who identified by taxonomic name the organism from which the sequence was obtained
+src-Isolate,Optional,Identification or description of the specific individual from which this sequence was obtained.
+src-Isolation-source,Optional,Describes the local geographical source of the organism from which the sequence was obtained.
+src-Lab_host,Optional,Laboratory host used to propagate the organism from which the sequence was obtained.
+src-Lat_Lon,Optional,"Latitude and longitude, in decimal degrees, of where the sample was collected."
+src-Note,Optional,Any additional information that you wish to provide about the sequence.
+src-Pathovar,Optional,"Variety of a species (usually a fungus, bacteria or virus) characterized by the biological target of the pathogen. Examples include Pseudomonas syringae pathovar tomato and Pseudomonas syringae pathovar tabaci."
+src-Pop_variant,Optional,name of the population variant from which the sequence was obtained
+src-Rev_primer_name,Optional,name of reverse PCR primer
+src-Rev_primer_seq,Optional,nucleotide sequence of reverse PCR primer
+src-Segment,Optional,name of viral or phage segment sequenced
+src-Serogroup,Optional,"Variety of a species (usually a fungus, bacteria, or virus) characterized by its antigenic properties. Same as serogroup and serovar."
+src-Serotype,Optional,See Serogroup
+src-Serovar,Optional,See Serogroup
+src-Sex,Optional,Sex of the organism from which the sequence was obtained.
+src-Specimen_voucher,Optional,"An identifier of the individual or collection of the source organism and the place where it is currently stored, usually an institution. This should be provided using the following format 'institution-code:collection-code:specimen-id'. specimen-id is mandatory, collection-code is optional; institution-code is mandatory when collection-code is provided. Examples: 99-SRNP UAM:Mamm:52179 personal collection:Joe Smith:99-SRNP AMCC:101706"
+src-Strain,Required,Strain of organism from which sequence was obtained.
+src-Sub_species,Optional,Subspecies of organism from which sequence was obtained.
+src-Subclone,Optional,Name of subclone from which sequence was obtained.
+src-Subtype,Optional,Subtype of organism from which sequence was obtained.
+src-Substrain,Optional,Sub-strain of organism from which sequence was obtained.
+src-Tissue_lib,Optional,Tissue library from which the sequence was obtained.
+src-Tissue_type,Optional,Type of tissue from which sequence was obtained.
+src-Type,Optional,Type of organism from which sequence was obtained.
+src-Variety,Optional,Variety of organism from which sequence was obtained.
diff --git a/shiny/templates/config.genbank.genbank.schema_template.csv b/shiny/templates/config.genbank.genbank.schema_template.csv
index 2574e40..fbae91e 100644
--- a/shiny/templates/config.genbank.genbank.schema_template.csv
+++ b/shiny/templates/config.genbank.genbank.schema_template.csv
@@ -1,8 +1,6 @@
column_name,required_column,description
sequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.
-gb-sample_name,Required,"Identifier name used for GenBank. Max length is 50 characters. Fasta modifiers with brackets ""[]"" can be added. They will be added only to the fasta file."
-gb-subm_lab,Required,"Full name of organization, institute, or laboratory, etc., who is submitting this record."
-gb-subm_lab_division,Required,"The division of organization, institute, or laboratory, etc., who is submitting this record."
-gb-subm_lab_addr,Required,"The address of organization, institute, or laboratory, etc., who is submitting this record."
-gb-publication_title,Optional,"The title and relevant publication details (volume, issue, etc.) of a paper that discusses the submission. If left empty, the program will used the name of the submission as title."
-gb-publication_status,Optional,"Options: ""unpublished"" or ""in-press"" or ""published""."
+gb-sample_name,Required,Identifier name used for GenBank. Max length is 50 characters.
+gb-fasta_definition_line_modifiers,Optional,"NCBI fasta definition line modifiers can be added here. As many modifiers as you like can be added, but each must bounded by a set of brackets. Some of the available keywords are listed at ""https://www.ncbi.nlm.nih.gov/genbank/mods_fastadefline/""."
+gb-title,Optional,"Optional internal field for how the GenBank submission should be named when viewed from the NCBI submission portal, . If not provided, when performing submissions <--submission_name> with the suffix ""-GB"" will be used instead."
+sra-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/shiny/templates/config.gisaid.gisaid.FLU.schema_template.csv b/shiny/templates/config.gisaid.gisaid.FLU.schema_template.csv
index 702f97f..ade3850 100644
--- a/shiny/templates/config.gisaid.gisaid.FLU.schema_template.csv
+++ b/shiny/templates/config.gisaid.gisaid.FLU.schema_template.csv
@@ -1,6 +1,7 @@
column_name,required_column,description
sequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank and/or GISAID.
-gs-sample_name,Required,Identifier name used for GISAID. Max length is 50 characters.
+gs-sample_name,Required,Identifier name for sample segment used for GISAID. Max length is 50 characters.
+gs-Isolate_Name,Required,Identifier name for sample used for GISAID. Max length is 50 characters.
gs-segment,Required,Segment name for sequence.
gs-Subtype,Required,Influenza subtype (e.g. H5N1).
gs-Lineage,Optional,Lineage information.
diff --git a/shiny/templates/config.seqsender.seqsender.schema_template.csv b/shiny/templates/config.seqsender.seqsender.schema_template.csv
new file mode 100644
index 0000000..d950c91
--- /dev/null
+++ b/shiny/templates/config.seqsender.seqsender.schema_template.csv
@@ -0,0 +1,6 @@
+column_name,required_column,description
+sequence_name,Required,Sequence identifier used in fasta file. This is used to create the fasta file for Genbank or GISAID.
+organism,Required,The most descriptive organism name for the samples.
+authors,Required,"Citing authors. List of Last, First Middle, suffix separated by a semicolon "";"" E.g.: ""Baker, Howard Henry, Jr.; Powell, Earl Alexander, III.;"""
+collection_date,Required,"Collection date for sample. Must be in a valid format based on ISO 8601: ""YYYY-MM-DD"", ""YYYY-MM"", or ""YYYY"". Time is not supported."
+bioproject,Optional,NCBI BioProject ID.
diff --git a/shiny/templates/config.seqsender.submission.status.report.biosample.submission.status.report.schema_template.csv b/shiny/templates/config.seqsender.submission.status.report.biosample.submission.status.report.schema_template.csv
new file mode 100644
index 0000000..9dc26c3
--- /dev/null
+++ b/shiny/templates/config.seqsender.submission.status.report.biosample.submission.status.report.schema_template.csv
@@ -0,0 +1,5 @@
+column_name,required_column,description
+bs-sample_name,Required,Sample name used for BioSample submission.
+biosample_status,Required,Current status of the BioSample submission.
+biosample_accession,Required,Sample accession ID used to identify BioSample submission NCBI.
+biosample_message,Required,"If there is a message tied to the sample submitted to BioSample, it will appear here."
diff --git a/shiny/templates/config.seqsender.submission.status.report.genbank.submission.status.report.schema_template.csv b/shiny/templates/config.seqsender.submission.status.report.genbank.submission.status.report.schema_template.csv
new file mode 100644
index 0000000..12853e4
--- /dev/null
+++ b/shiny/templates/config.seqsender.submission.status.report.genbank.submission.status.report.schema_template.csv
@@ -0,0 +1,5 @@
+column_name,required_column,description
+gb-sample_name,Required,Sample name used for GenBank submission.
+genbank_status,Required,Current status of the GenBank submission.
+genbank_accession,Required,Sample accession ID used to identify GenBank submission NCBI.
+genbank_message,Required,"If there is a message tied to the sequence submitted to GenBank, it will appear here."
diff --git a/shiny/templates/config.seqsender.submission.status.report.gisaid.submission.status.report.schema_template.csv b/shiny/templates/config.seqsender.submission.status.report.gisaid.submission.status.report.schema_template.csv
new file mode 100644
index 0000000..deb6034
--- /dev/null
+++ b/shiny/templates/config.seqsender.submission.status.report.gisaid.submission.status.report.schema_template.csv
@@ -0,0 +1,6 @@
+column_name,required_column,description
+gs-sample_name,Required,Sample name used for GISAID submission.
+gs-segment_name,Optional,Segment name of sample used for GISAID submission.
+gisaid_accession_epi_isl_id,Required,Sample accession ID used to identify submission to GISAID.
+gisaid_accession_epi_id,Required,Sample accession ID used to identify submission in GISAID.
+gisaid_message,Required,"If there is a message tied to the sequence submitted to GISAID, it will appear here."
diff --git a/shiny/templates/config.seqsender.submission.status.report.sra.submission.status.report.schema_template.csv b/shiny/templates/config.seqsender.submission.status.report.sra.submission.status.report.schema_template.csv
new file mode 100644
index 0000000..f7a55c3
--- /dev/null
+++ b/shiny/templates/config.seqsender.submission.status.report.sra.submission.status.report.schema_template.csv
@@ -0,0 +1,5 @@
+column_name,required_column,description
+sra-sample_name,Required,Sample name used for SRA submission.
+sra_status,Required,Current status of the SRA submission.
+sra_accession,Required,Sample accession ID used to identify SRA submission NCBI.
+sra_message,Required,"If there is a message tied to the sequence submitted to SRA, it will appear here."
diff --git a/shiny/templates/config.sra.sra.schema_template.csv b/shiny/templates/config.sra.sra.schema_template.csv
new file mode 100644
index 0000000..32fd1b1
--- /dev/null
+++ b/shiny/templates/config.sra.sra.schema_template.csv
@@ -0,0 +1,16 @@
+column_name,required_column,description
+sra-sample_name,Required,Identifier name used for SRA. Max length is 50 characters. Name must be unique from BioSample or Genbank submission.
+bs-sample_name,Required,Identifier name used for BioSample. Max length is 50 characters. Required if submitting to SRA.
+sra-file_location,Required,"Location of raw reads files. Options: ""local"" or ""cloud""."
+sra-file_[1-9]\d*,Required,"Name of the raw read files. All file names must be unique and not contain any sensitive information. Files can be compressed using gzip or bzip2, and may be submitted in a tar archive but archiving and/or compressing your files is not required. Do not use zip! If there are multiple files, concatenate them with a commas ("",""), e.g. ""sample1_R1.fastq.gz, sample1_R2.fastq.gz"". Store files in /seqsender/data/raw_reads/ or provide full html path to the raw read files."
+sra-library_name,Optional,Short unique identifier for sequencing library.
+sra-loader,Optional,"SRA loader selection. If NCBI says your SRA files need to be uploaded with a certain loader, you can select it here."
+sra-library_strategy,Required,The sequencing technique intended for the library.
+sra-library_source,Required,The type of source material that is being sequenced.
+sra-library_selection,Required,The method used to select and/or enrich the material being sequenced.
+sra-library_layout,Required,"Whether to expect SINGLE or PAIRED end reads. Options: ""single"" or ""paired""."
+sra-platform,Optional,Instrument platform.
+sra-instrument_model,Required,Instrument model used for sequencing.
+sra-design_description,Optional,Brief description of materials/methods used for creating sequencing library.
+sra-title,Optional,"Optional internal field for how the SRA submission should be named when viewed from the NCBI submission portal. If not provided, when performing submissions <--submission_name> with the suffix ""-SRA"" will be used instead."
+sra-comment,Optional,Optional internal field explaining the purpose of the submission for when interacting and resolving submission issues with NCBI.
diff --git a/submission_log.csv b/submission_log.csv
deleted file mode 100755
index 32eb152..0000000
--- a/submission_log.csv
+++ /dev/null
@@ -1,7 +0,0 @@
-Submission_Name,Organism,Database,Submission_Type,Submission_Date,Submission_Status,Submission_ID,Submission_Directory,Config_File,Update_Date
-COV_TEST_DATA,COV,BIOSAMPLE,TEST,2024-08-05,PROCESSED,SUB921446,/scicomp/home-pure/psv4/seqsender_dev/seqsender/COV_TEST_DATA/submission_files/BIOSAMPLE,/scicomp/home-pure/psv4/seqsender_dev/seqsender/COV_TEST_DATA/config.yaml,2024-08-05
-COV_TEST_DATA,COV,GENBANK-FTP,TEST,2024-08-05,PROCESSED,SUB921490,/scicomp/home-pure/psv4/seqsender_dev/seqsender/COV_TEST_DATA/submission_files/GENBANK,/scicomp/home-pure/psv4/seqsender_dev/seqsender/COV_TEST_DATA/config.yaml,2024-08-06
-FLU_TEST_DATA,FLU,BIOSAMPLE,TEST,2024-08-05,PROCESSED,SUB921448,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/submission_files/BIOSAMPLE,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/config.yaml,2024-08-05
-FLU_TEST_DATA,FLU,SRA,TEST,2024-08-05,ERROR,SUB921447,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/submission_files/SRA,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/config.yaml,2024-08-06
-FLU_TEST_DATA,FLU,GENBANK-FTP,TEST,2024-08-06,FAILED,PENDING,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/submission_files/GENBANK,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/config.yaml,2024-08-06
-FLU_TEST_DATA,FLU,GENBANK-TBL2ASN,TEST,2024-08-06,PROCESSED,PENDING,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/submission_files/GENBANK,/scicomp/home-pure/psv4/seqsender_dev/seqsender/FLU_TEST_DATA/config.yaml,2024-08-06
diff --git a/test_data/COV/cov_biosample_metadata.csv b/test_data/COV/cov_biosample_metadata.csv
index 681afd9..1eb715f 100755
--- a/test_data/COV/cov_biosample_metadata.csv
+++ b/test_data/COV/cov_biosample_metadata.csv
@@ -1,3 +1,3 @@
-organism,collection_date,authors,bioproject,bs-sample_name,bs-isolate,bs-description,bs-collected_by, bs-host, bs-host_disease,bs-isolation_source,bs-geo_loc_name,bs-host_sex,bs-host_age
-Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,SARS-CoV-2/human/USA/GA_2741/2020,SARS-CoV-2/human/USA/GA_2741/2020,Sars CoV2 Sequencing Baseline Constellation,Helix,Homo sapiens,COVID-19,nasal swab,United States: Georgia,Male,28
-Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,SARS-CoV-2/human/USA/GA_3742/2020,SARS-CoV-2/human/USA/GA_3742/2020,Sars CoV2 Sequencing Baseline Constellation,Helix,Homo sapiens,COVID-20,nasal swab,United States: Georgia,Male,45
+organism,collection_date,authors,bioproject,bs-sample_name,bs-isolate,bs-sample_title,bs-collected_by, bs-host, bs-host_disease,bs-isolation_source,bs-geo_loc_name,bs-host_sex,bs-host_age,bs-title,bs-comment
+Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,SARS-CoV-2/human/USA/GA_2741/2020,SARS-CoV-2/human/USA/GA_2741/2020,Sars CoV2 Sequencing Baseline Constellation,Helix,Homo sapiens,COVID-19,nasal swab,United States: Georgia,Male,28,cov_test_bs_submission,test biosample cov submission
+Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,SARS-CoV-2/human/USA/GA_3742/2020,SARS-CoV-2/human/USA/GA_3742/2020,Sars CoV2 Sequencing Baseline Constellation,Helix,Homo sapiens,COVID-20,nasal swab,United States: Georgia,Male,45,cov_test_bs_submission,test biosample cov submission
diff --git a/test_data/COV/cov_config.yaml b/test_data/COV/cov_config.yaml
index af1be64..f183dee 100644
--- a/test_data/COV/cov_config.yaml
+++ b/test_data/COV/cov_config.yaml
@@ -11,8 +11,6 @@ Submission:
Specified_Release_Date: ""
Link_Sample_Between_NCBI_Databases: True
Description:
- Title: cov_test_submission
- Comment: This is a test submission.
Organization:
Role: owner
Type: institute
diff --git a/test_data/COV/cov_genbank_metadata.csv b/test_data/COV/cov_genbank_metadata.csv
index 58059d3..1fa7830 100755
--- a/test_data/COV/cov_genbank_metadata.csv
+++ b/test_data/COV/cov_genbank_metadata.csv
@@ -1,3 +1,3 @@
-sequence_name,organism,collection_date,authors,bioproject,gb-sample_name,src-isolate,src-country,src-host,src-isolation_source,cmt-StructuredCommentPrefix,cmt-Assembly Method,cmt-Coverage,cmt-Sequencing Technology,cmt-StructuredCommentSuffix
-GA_2741,Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,SARS-CoV-2/human/USA/GA_2741/2020,SARS-CoV-2/human/USA/GA_2741/2020,USA: GA,Homo sapiens,nasal swab,Assembly-Data,Newbler v. 2.3,100x,Illumina ,Assembly-Data
-GA_2742,Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,SARS-CoV-2/human/USA/GA_3742/2020,SARS-CoV-2/human/USA/GA_3742/2020,USA: GA,Homo sapiens,nasal swab,Assembly-Data,Newbler v. 2.3,100x,Illumina,Assembly-Data
+sequence_name,organism,collection_date,authors,bioproject,gb-sample_name,gb-fasta_definition_line_modifiers,src-isolate,src-country,src-host,src-isolation_source,cmt-StructuredCommentPrefix,cmt-Assembly Method,cmt-Coverage,cmt-Sequencing Technology,cmt-StructuredCommentSuffix,gb-title,gb-comment
+GA_2741,Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,SARS-CoV-2/human/USA/GA_2741/2020,[keyword=purposeofsampling:baselinesurveillance],SARS-CoV-2/human/USA/GA_2741/2020,USA: GA,Homo sapiens,nasal swab,Assembly-Data,Newbler v. 2.3,100x,Illumina,Assembly-Data,cov_test_gb_submission,test genbank cov submission
+GA_2742,Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,SARS-CoV-2/human/USA/GA_3742/2020,[keyword=purposeofsampling:baselinesurveillance],SARS-CoV-2/human/USA/GA_3742/2020,USA: GA,Homo sapiens,nasal swab,Assembly-Data,Newbler v. 2.3,100x,Illumina,Assembly-Data,cov_test_gb_submission,test genbank cov submission
diff --git a/test_data/COV/cov_gisaid_metadata.csv b/test_data/COV/cov_gisaid_metadata.csv
index 94d5604..235aad4 100755
--- a/test_data/COV/cov_gisaid_metadata.csv
+++ b/test_data/COV/cov_gisaid_metadata.csv
@@ -1,3 +1,3 @@
sequence_name,organism,collection_date,authors,gs-sample_name,gs-covv_type,gs-covv_passage,gs-covv_location,gs-covv_add_location,gs-covv_host,gs-covv_add_host_info,gs-covv_sampling_strategy,gs-covv_gender,gs-covv_patient_age,gs-covv_patient_status,gs-covv_specimen,gs-covv_outbreak,gs-covv_last_vaccinated,gs-covv_treatment,gs-covv_seq_technology,gs-covv_assembly_method,gs-covv_coverage,gs-covv_orig_lab,gs-covv_orig_lab_addr,gs-covv_provider_sample_id,gs-covv_subm_lab,gs-covv_subm_lab_addr,gs-covv_subm_sample_id,gs-covv_consortium,gs-covv_comment,gs-comment_type
-GA_2741,Severe acute respiratory syndrome coronavirus 2,3/28/2020,John Doe; Jane Doe;,hCoV-19/USA/GA-2741/2022,betacoronavirus,Original,North America/USA/Georgia,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"32 Data St, Mel, Aus",,,,
-GA_2742,Severe acute respiratory syndrome coronavirus 2,4/29/2020,John Doe; Jane Doe;,hCoV-19/USA/GA_2742/2022,betacoronavirus,Original,North America/USA/Georgia,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"33 Data St, Mel, Aus",,,,
+GA_2741,Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,hCoV-19/USA/GA-2741/2020,betacoronavirus,Original,North America/USA/Georgia,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"32 Data St, Mel, Aus",,,,
+GA_2742,Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,hCoV-19/USA/GA_2742/2020,betacoronavirus,Original,North America/USA/Georgia,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"33 Data St, Mel, Aus",,,,
diff --git a/test_data/COV/cov_sra_metadata.csv b/test_data/COV/cov_sra_metadata.csv
index c7112b7..1e0f322 100755
--- a/test_data/COV/cov_sra_metadata.csv
+++ b/test_data/COV/cov_sra_metadata.csv
@@ -1,3 +1,3 @@
-sra-sample_name,organism,collection_date,authors,bioproject,sra-file_location,sra-file_1,sra-file_2,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-loader
-SARS-CoV-2/human/USA/GA_2741/2020,Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,local,fastq_1_R1.fastq.gz, fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-SARS-CoV-2/human/USA/GA_3742/2020,Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,local,fastq_2_R1.fastq.gz, fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
+sra-sample_name,bs-sample_name,organism,collection_date,authors,bioproject,sra-file_location,sra-file_1,sra-file_2,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-loader,sra-title,sra-comment
+SARS-CoV-2/human/USA/GA_2741,SARS-CoV-2/human/USA/GA_2741/2020,Severe acute respiratory syndrome coronavirus 2,2020-03-28,John Doe; Jane Doe;,PRJNA512913,local,fastq_1_R1.fastq.gz, fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_COV_sra_submission,test sra submission
+SARS-CoV-2/human/USA/GA_3742,SARS-CoV-2/human/USA/GA_2741/2020,Severe acute respiratory syndrome coronavirus 2,2020-04-29,John Doe; Jane Doe;,PRJNA512962,local,fastq_2_R1.fastq.gz, fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_COV_sra_submission,test sra submission
diff --git a/test_data/FLU/flu_biosample_metadata.csv b/test_data/FLU/flu_biosample_metadata.csv
index 1b668a0..5be3d79 100755
--- a/test_data/FLU/flu_biosample_metadata.csv
+++ b/test_data/FLU/flu_biosample_metadata.csv
@@ -1,17 +1,17 @@
-bs-sample_name,organism,collection_date,authors,bioproject,bs-description,bs-collected_by,bs-geo_loc_name,bs-strain, bs-host, bs-host_disease,bs-isolate,bs-isolation_source,bs-lat_lon,bs-host_sex,bs-host_age,bs-serotype
-XX-566912_PB2,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_PB1,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_PA,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_HA,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_NP,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_NA,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_M,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566912_NS,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2
-XX-566913_PB2,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_PB1,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_PA,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_HA,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_NP,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_NA,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_M,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
-XX-566913_NS,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,CDC Influenza Sequencing Baseline Constellation,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2
+bs-sample_name,organism,collection_date,authors,bioproject,bs-collected_by,bs-geo_loc_name,bs-strain, bs-host, bs-host_disease,bs-isolate,bs-isolation_source,bs-lat_lon,bs-host_sex,bs-host_age,bs-serotype,bs-title,bs-comment
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/California/566912/2016,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,Helix,USA: California,Influenza A,Homo sapiens,Influenza A viruses,A/California/566912/2016,Nasal Wash,36.78 N 119.42 W,female,92,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
+A/Texas/566913/2016,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,Helix,USA: Texas,Influenza A,Homo sapiens,Influenza A viruses,A/Texas/566913/2016,Nasal Wash,29.42 N 98.49 W,male,21,H3N2,flu_test_bs_submission,test biosample flu submission
diff --git a/test_data/FLU/flu_config.yaml b/test_data/FLU/flu_config.yaml
index 0d00920..2361580 100644
--- a/test_data/FLU/flu_config.yaml
+++ b/test_data/FLU/flu_config.yaml
@@ -11,8 +11,6 @@ Submission:
Specified_Release_Date: "2 weeks"
Link_Sample_Between_NCBI_Databases: True
Description:
- Title: flu_test_submission
- Comment: This is a test submission
Organization:
Role: owner
Type: institute
diff --git a/test_data/FLU/flu_sra_metadata.csv b/test_data/FLU/flu_sra_metadata.csv
index 71afaaf..3def57e 100755
--- a/test_data/FLU/flu_sra_metadata.csv
+++ b/test_data/FLU/flu_sra_metadata.csv
@@ -1,17 +1,17 @@
-sra-sample_name,organism,collection_date,authors,bioproject,sra-file_location,sra-file_1, sra-file_2,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-sra_loader
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
+sra-sample_name,organism,collection_date,authors,bioproject,bs-sample_name,sra-file_location,sra-file_1, sra-file_2,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-sra_loader,sra-title,sra-comment
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566912,Influenza A virus,2016-12-28,John Doe; Jane Doe;,PRJNA217342,A/California/566912/2016,local,fastq_1_R1.fastq.gz,fastq_1_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
+XX-566913,Influenza A virus,2016-11-10,John Doe; Jane Doe;,PRJNA217342,A/Texas/566913/2016,local,fastq_2_R1.fastq.gz,fastq_2_R2.fastq.gz,Helix COVID-19 and Flu Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load,test_FLU_sra_submission,test sra submission
diff --git a/test_data/OTHER/other_biosample_metadata.csv b/test_data/OTHER/other_biosample_metadata.csv
deleted file mode 100755
index b3ed65a..0000000
--- a/test_data/OTHER/other_biosample_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-ncbi-spuid,ncbi-spuid_namespace,bs-description,organism,ncbi-bioproject,bs-isolate,bs-collected_by,bs-package, bs-collection_date, bs-host, bs-host_disease,bs-isolation_source,bs-geo_loc_name,bs-lat_lon,bs-host_sex,bs-host_age
-seq1,CDC-OAMD,CDC Test,Monkeypox virus,PRJNA512913,MPXV/human/USA/GA_2741/2020,Helix,Pathogen.cl.1.0,3/28/2020,Homo sapiens,Monkeypox virus,nasal swab,United States: Georgia,Unknown,Male,28
-seq2,CDC-OAMD,CDC Test,Monkeypox virus,PRJNA512962,MPXV/human/USA/GA_3742/2020,Helix,Pathogen.cl.1.0,4/29/2020,Homo sapiens,Monkeypox virus,nasal swab,United States: Georgia,Unknown,Male,45
diff --git a/test_data/OTHER/other_config.yaml b/test_data/OTHER/other_config.yaml
deleted file mode 100755
index eb54a0a..0000000
--- a/test_data/OTHER/other_config.yaml
+++ /dev/null
@@ -1,35 +0,0 @@
-Submission:
- NCBI:
- Username: username
- Password: password
- BioSample_Package: Pathogen.cl.1.0
- Submission_Position: 1
- Description:
- Title: test_submission
- Comment: This is a test submission
- Organization:
- '@role': owner
- '@type': institute
- '@org_id': 12345
- Name: CDC
- Address:
- Affil: Centers for Disease Control and Prevention
- Div: Respiratory Viruses Branch, Division of Viral Diseases
- Street: 1600 Clifton Rd
- City: Atlanta
- Sub: GA
- Postal_code: 30329
- Country: USA
- Email: email@myemail.com
- Phone: ""
- Submitter:
- '@email': email@myemail.com
- '@alt_email':
- Name:
- First: Jane
- Last: Doe
- GISAID:
- Client-Id: TEST-EA76875B00C3
- Username: username
- Password: password
- Submission_Position: 2
diff --git a/test_data/OTHER/other_fastq_1_R1.fastq.gz b/test_data/OTHER/other_fastq_1_R1.fastq.gz
deleted file mode 100755
index 4f1c470..0000000
Binary files a/test_data/OTHER/other_fastq_1_R1.fastq.gz and /dev/null differ
diff --git a/test_data/OTHER/other_fastq_1_R2.fastq.gz b/test_data/OTHER/other_fastq_1_R2.fastq.gz
deleted file mode 100755
index 76cb481..0000000
Binary files a/test_data/OTHER/other_fastq_1_R2.fastq.gz and /dev/null differ
diff --git a/test_data/OTHER/other_fastq_2_R1.fastq.gz b/test_data/OTHER/other_fastq_2_R1.fastq.gz
deleted file mode 100755
index 4f1c470..0000000
Binary files a/test_data/OTHER/other_fastq_2_R1.fastq.gz and /dev/null differ
diff --git a/test_data/OTHER/other_fastq_2_R2.fastq.gz b/test_data/OTHER/other_fastq_2_R2.fastq.gz
deleted file mode 100755
index 76cb481..0000000
Binary files a/test_data/OTHER/other_fastq_2_R2.fastq.gz and /dev/null differ
diff --git a/test_data/OTHER/other_genbank_metadata.csv b/test_data/OTHER/other_genbank_metadata.csv
deleted file mode 100755
index 0641ebb..0000000
--- a/test_data/OTHER/other_genbank_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-sequence_name,ncbi-spuid,ncbi-spuid_namespace,gb-subm_lab,gb-subm_lab_division,gb-subm_lab_addr,authors,gb-publication_status,gb-publication_title,organism,gb-seq_id,src-isolate,collection_date,src-country,src-host,src-isolation_source,src-serotype,src-BioProject,src-BioSample,cmt-StructuredCommentPrefix,cmt-Assembly Method,cmt-Coverage,cmt-Sequencing Technology,cmt-StructuredCommentSuffix
-seq1,seq1,CDC-OAMD,NIH,NCBI,"10 Center Dr, Bethesda, MD, USA 20895","Doe, John, R.; Doe, Jane;",unpublished,,Monkeypox virus,MPXV/human/USA/GA_2741/2020,MPXV/human/USA/GA_2741/2020,3/28/2020,USA: GA,Homo sapiens,missing,Clade 1,PRJNA512913,SAMN02224951,Assembly-Data,Newbler v. 2.3,100x,Illumina ,Assembly-Data
-seq2,seq2,CDC-OAMD,NIH,NCBI,"10 Center Dr, Bethesda, MD, USA 20895","Doe, John; Doe, Jane;",unpublished,,Monkeypox virus,MPXV/human/USA/GA_3742/2020,MPXV/human/USA/GA_3742/2020,4/29/2020,USA: GA,Homo sapiens,missing,Clade 1,PRJNA512962,SAMN02224986,Assembly-Data,Newbler v. 2.3,100x,Illumina,Assembly-Data
diff --git a/test_data/OTHER/other_sequence.fasta b/test_data/OTHER/other_sequence.fasta
deleted file mode 100755
index 995e77d..0000000
--- a/test_data/OTHER/other_sequence.fasta
+++ /dev/null
@@ -1,4244 +0,0 @@
->hCoV-19/Anzark/12/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAATATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGTGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACANNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNNNAAAAAA
-AAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/13/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAATATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGTGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATNNNNNNNNNAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/14/2022
-CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCAC
-TCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGG
-TTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGT
-CCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTG
-GAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAA
-GGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTAT
-GGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGG
-GCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCC
-GATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACA
-TAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTG
-GCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAA
-CTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTC
-TGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAA
-ATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGT
-AGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCA
-TTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAG
-AAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTA
-GGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGC
-CTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAG
-GTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAA
-GTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAG
-TGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTA
-CAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCA
-GAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGC
-CGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA
-CTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGC
-ACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGG
-TTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTA
-AGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCT
-AAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGA
-AGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAGTTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGT
-TAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAATCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTG
-GTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAA
-TATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAG
-AAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAGGTGCTCT
-GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACC
-AGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTG
-GTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAA
-GAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCAC
-TTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAG
-ACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCA
-GTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACAT
-TGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTG
-CAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAA
-GTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGG
-TGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTG
-GTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTT
-GATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGA
-GATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAA
-TCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGAC
-ATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATA
-TATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAA
-TGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACT
-GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCA
-AGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCT
-GTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTAT
-GGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCT
-TGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGC
-CAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAA
-GAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTAT
-AGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCA
-CCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAAC
-CTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTAC
-TAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTT
-TTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATAC
-CCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACA
-AATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTG
-CACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACAT
-GCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGT
-AGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAAC
-AAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCAT
-GGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT
-GTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACA
-GTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAAT
-TATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTT
-CGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTT
-CAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCT
-TTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACC
-AAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGG
-ACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAG
-AAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAA
-AATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATG
-AATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATA
-GCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTG
-TACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAG
-CATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTG
-AAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTA
-CTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGA
-ACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTA
-GACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGC
-AGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCA
-GCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCA
-GCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAA
-TTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAA
-GGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTC
-TGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGA
-CCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGA
-CTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATT
-AATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTAT
-GTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTG
-ATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCT
-GAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGA
-TGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATA
-ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCAT
-ATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACT
-ACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTA
-ATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTT
-GTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGA
-AATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATG
-CTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATA
-ACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTT
-ACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAG
-CTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTA
-CTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCC
-TAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGAT
-CAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTC
-TGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGC
-ATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTG
-GTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTAC
-TCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACA
-TATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGC
-ATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCT
-GCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAA
-TAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTT
-GTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACC
-TCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTG
-TGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACA
-TGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTC
-AGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAA
-GTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTG
-CTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGAC
-TGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTA
-TGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGT
-ACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATG
-AAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTT
-AGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAG
-ATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGT
-ACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTT
-TTTTTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATA
-AGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGT
-TGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTA
-TGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTA
-TGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATC
-TCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTA
-TTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTT
-GTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAG
-GAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTT
-GGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCT
-TACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATT
-CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGT
-AGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTC
-CATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAA
-AAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGC
-TGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA
-TGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCC
-TTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGA
-TGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTA
-GTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAA
-TTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGA
-TGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGA
-AATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGAC
-ACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAG
-TTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTT
-TTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTG
-TGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATC
-GTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAA
-TACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGT
-TATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGT
-GTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGA
-TAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTG
-ATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGT
-CCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTAC
-TAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTG
-TCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGC
-GTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGG
-TATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGC
-CAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCA
-GAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTT
-AAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCA
-TTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATA
-TTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAA
-CTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATC
-TATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGT
-AATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTT
-CTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTATTATCGTTATAATCTACCAACAATGTGTGATATCA
-GACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTC
-ATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAG
-TTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATG
-CCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAA
-AAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAA
-CATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGC
-CTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTAT
-AGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAAC
-CTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCAC
-TTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGA
-AATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGA
-CGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTT
-ATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGC
-TCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGC
-CGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTT
-ACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGAT
-GAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTT
-TTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA
-GATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTA
-GTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT
-GAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAA
-ATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATT
-TTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTC
-TTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCAC
-CACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAA
-AAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGAC
-ATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAA
-CACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAG
-GGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGC
-TTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATAC
-CTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAAT
-GCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAA
-TGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGG
-GCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGT
-CGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATC
-AGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCG
-TGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTA
-GCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCA
-AACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCA
-TAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAA
-GCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCT
-CAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGAC
-TCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATA
-AGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTT
-ACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTT
-CCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAAT
-GTAGTGTGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGC
-ACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTG
-CCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTT
-ATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGC
-ACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTA
-TTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCA
-TTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGA
-ATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATT
-CTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGA
-TTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCA
-CACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGT
-CTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGT
-GGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAG
-CTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTT
-TTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACA
-AAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAA
-GCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGG
-ACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAA
-ACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGG
-TGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACAT
-TAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTAC
-TTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGA
-ATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTG
-GTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGT
-ACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGA
-TGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAA
-TTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCG
-GGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGC
-AACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTG
-TACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAG
-TGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGA
-TTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAA
-AAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTG
-GCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGT
-TACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATG
-GTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATG
-AGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCT
-TCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGA
-ACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCC
-TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG
-ACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTT
-GATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTT
-TGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAAT
-TTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTT
-TATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTT
-CAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAG
-TGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACANNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAG
-ATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCAT
-CATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCA
-TTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACC
-AGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGT
-ATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCT
-TGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCA
-ACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATT
-TGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTT
-CTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGA
-CATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATC
-AGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGT
-TCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCAT
-TGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCA
-TTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACT
-ATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTC
-AACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTG
-AACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGT
-TTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGT
-GACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCAC
-AAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCG
-GGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTT
-TAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCA
-AAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTA
-AACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAA
-AGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAA
-TTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGA
-GTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGAC
-TTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAG
-GTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAAC
-ACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGA
-CTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTA
-ATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGAT
-CTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGC
-CATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCT
-GCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATT
-TGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTT
-CGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTT
-TCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACT
-TGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTAT
-GCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAA
-AAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTG
-TAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTAT
-ACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTC
-AACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAAC
-ATGTCCAAATTCACACAATCGACGTTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACG
-ACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTT
-AATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGAT
-TGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTG
-AATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTA
-GCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTT
-CCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTT
-TCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGA
-ATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTAC
-GCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGC
-TTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGT
-GACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCG
-TGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTA
-GCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGA
-GATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATT
-TATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAA
-ATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGT
-ACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCAC
-TGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTT
-TCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT
-TATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGC
-CTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTC
-ACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTA
-CAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGT
-AGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATA
-TCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGT
-TCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCT
-GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAA
-TGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTC
-TCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGAC
-CAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTA
-TTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTG
-AGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCT
-CAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACG
-TAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTG
-ATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAA
-GGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCCAAATTTCAAAGATCA
-AGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG
-CTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGAT
-TTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGAT
-GGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAG
-CACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCC
-ACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGA
-GCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTNNNNNNNNNNNNNCAAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/16/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTNNNN
-NNNNNNNNNATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCA
-CTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAG
-AGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACGCACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/17/2022
-CAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTG
-CACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTA
-CGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCT
-TGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCT
-TTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAA
-AAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGT
-TATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATG
-TGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGC
-GCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAA
-ACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCT
-GTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAA
-CAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACG
-TTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTC
-CAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATG
-GGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGA
-TCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTA
-AAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAA
-GTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTAT
-TGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACA
-TAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAG
-AAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCAC
-AAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAG
-TTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCA
-TCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAA
-GGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGG
-CTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTT
-GGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGA
-CGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAA
-TTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGA
-GCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAG
-AGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAG
-TGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCA
-TTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACC
-TAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGA
-TAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGC
-TCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCA
-ACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGT
-CTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAA
-GAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGC
-CACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAAC
-AAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACA
-CCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGA
-CATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTG
-TTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTT
-AAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAA
-AGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAG
-CTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC
-TTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGC
-TGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGA
-AAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATT
-GACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCC
-ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTG
-AAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTAC
-ACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAA
-GCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTG
-TCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGAT
-TATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAAC
-TCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAG
-TGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCT
-GAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGG
-TATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTA
-TCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATT
-AACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGT
-TACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGG
-CTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAA
-TACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCA
-ACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTT
-GTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAA
-CATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGG
-TGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTA
-AACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAG
-CATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAAC
-TTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAA
-ACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGAC
-AATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAG
-CTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTG
-CTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCC
-TCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAA
-ACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAG
-AGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATA
-CAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTT
-AAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTA
-ATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACT
-ATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGT
-TTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTA
-AAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTAT
-TTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAAT
-CTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATT
-TGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCT
-TTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGT
-TGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTT
-TCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATT
-TCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTG
-TAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA
-GAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACA
-TTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTAC
-TGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAA
-AGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCT
-ATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCT
-TATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGT
-TTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAA
-GCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTC
-AGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTA
-ATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGT
-CATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACA
-ACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTG
-TTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACA
-CTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAG
-TGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAAC
-ATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTC
-ATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTT
-CTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACAT
-CAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAAT
-GTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATT
-TCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAA
-GATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTT
-TTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATC
-AGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTT
-TTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTT
-TACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGC
-ACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAA
-AGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAA
-GCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATA
-TAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTT
-GTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATC
-ACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAAC
-TTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAG
-ACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAA
-CTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTA
-TAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAAT
-GTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTAT
-GACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTT
-TTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGT
-TGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCT
-ATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGT
-TTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAG
-AAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAG
-GGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTT
-TTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAAC
-ATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCT
-AGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTAT
-GTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACAC
-TTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATA
-ATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGA
-GTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTA
-CTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACA
-CAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATT
-GTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAG
-TCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGAC
-ATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGC
-TGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCC
-TTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTT
-AAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGAT
-GGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGA
-CAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTT
-CCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTG
-TGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAAC
-TTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTC
-AAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCAC
-TGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATT
-TGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACA
-GACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGG
-TAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTG
-CTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATG
-TTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGC
-ATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTAC
-AAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAA
-GGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGC
-GGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAA
-TGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAA
-TTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGAT
-TGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCT
-TACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATAC
-TTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTA
-CGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGC
-TGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCA
-CGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACT
-GCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAG
-GTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGAT
-GCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAA
-ATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGT
-AAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTA
-ATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCC
-GGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACA
-CTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATA
-TCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAA
-GTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAAT
-GAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGT
-ATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCAT
-CAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCA
-CAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCA
-TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTC
-TATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGG
-AACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG
-CACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTAT
-AGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTC
-TGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTC
-TTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTT
-TGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGG
-GGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATG
-CTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACAT
-GATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGA
-GTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCAT
-TAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAA
-TTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGG
-TATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATA
-AAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTAC
-ATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACT
-GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGAC
-CACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTT
-GAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCT
-GACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACC
-CAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTC
-CAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATAC
-AGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA
-TACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTA
-AATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGT
-CAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTA
-AGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACT
-TGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAA
-ATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAG
-GCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCT
-GTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCAC
-TCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTT
-GCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA
-CAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACA
-CCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAA
-GACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCT
-ATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACC
-TTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATT
-TTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGG
-AATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATG
-GGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGAC
-GTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCG
-TTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAA
-TGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGA
-CTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCT
-GCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGT
-AGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACAC
-ATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGT
-AGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATT
-CCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTG
-AGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTA
-GGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTT
-TAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGG
-CTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTAC
-ACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGC
-TAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCT
-GGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACT
-GAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAA
-TGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCA
-CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACT
-TACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGA
-TGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAG
-GTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGAC
-AGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACT
-TGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAG
-AAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAA
-CCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAG
-TGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAG
-CTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGA
-CAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGG
-TGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTA
-CAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCC
-GTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTT
-TGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAG
-ATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGAC
-ATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATC
-TCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAA
-CGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC
-CCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTC
-AGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGG
-TTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGAT
-TTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG
-AATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGA
-GTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAA
-TTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATT
-TAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTT
-CAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTA
-TTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTG
-CACTTGACCCTCTCTCAGAAACAAAGTGTANNNNNNNNNNNNNCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTT
-AGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCAC
-CAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCG
-CATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGAT
-TCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATT
-ACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACC
-TGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACA
-CCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTA
-CCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTA
-ATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAG
-TTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCT
-TGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTT
-ATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACA
-GGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACC
-CATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCA
-TCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTT
-ACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGA
-TTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTG
-TTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGT
-GGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAA
-AGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTG
-CACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTA
-GCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAG
-GTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTG
-GCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCT
-TTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGA
-CAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAAT
-TAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAA
-AGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGT
-GACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTG
-AAGGTGTCTTTGTTTCAAATGGCACGCACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGAC
-AACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATT
-AGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCA
-TTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATC
-GATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGAT
-TGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGAT
-CCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGG
-ATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTT
-GTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGT
-TTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCA
-ACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTT
-TATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTC
-CAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATA
-GTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGT
-TATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTA
-CTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAG
-AACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACG
-ACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTAC
-GTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTC
-GATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAAT
-CTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATT
-TTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGG
-TTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAA
-TTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGT
-GGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCG
-TACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGAC
-CGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGC
-TGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCA
-GCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCA
-GTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGC
-AGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAA
-ATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATG
-AAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAAC
-AGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTG
-CACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCA
-GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGT
-GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTT
-AGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT
-GTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGT
-TTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAG
-AGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCG
-ATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGT
-TGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATG
-TCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCA
-GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCG
-CTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGAT
-GACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATG
-GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAA
-CTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTT
-CCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATC
-ACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCG
-GTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAA
-CAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATA
-CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAG
-GAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGC
-ATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGA
-TCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGA
-AGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGAT
-GATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCA
-GATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACA
-TAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGA
-GCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGA
-AGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGC
->hCoV-19/x/Melbourne390/2022
-ACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGC
-TTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGC
-TGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGA
-GAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTAC
-GTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAA
-GTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGG
-TCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCC
-CTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGT
-TACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAA
-CACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACA
-ACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTG
-TCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACAC
-GGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGG
-AATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGC
-TTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAA
-GTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATT
-TGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAAT
-TCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCG
-CACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCG
-CTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAA
-AAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGC
-TTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATT
-TTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCA
-TTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTT
-ACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTG
-ATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAAC
-ATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCT
-TAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAA
-AGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATT
-GGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAA
-ATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCA
-CAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAA
-GCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCT
-TGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACA
-CTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAG
-AAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAAC
-TTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTG
-ATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGAT
-TGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT
-TGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTG
-GTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAA
-CTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAA
-TGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAG
-GAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCNNNNNNNNNNNNNNNNNCTAATGGA
-CCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGT
-TAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTAT
-TATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTA
-GCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAA
-GATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATG
-ATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTT
-TATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGA
-TGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCA
-CTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAAT
-GGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAA
-TGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAA
-TGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTG
-GTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAA
-TGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTC
-TCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAA
-ACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACA
-ACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTG
-AAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGAC
-AACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGC
-TGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTG
-TTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAG
-TGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTG
-TTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCT
-TAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGT
-GTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAA
-CTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAA
-AGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACA
-AAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAG
-TTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAA
-CGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGA
-AACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTAC
-ACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCAC
-GTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGAAAAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGA
-AGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCT
-ACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAA
-TAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGA
-AACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGG
-GATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAA
-CCGTGTTTGTACTAATTATATGCCNNNNNNNNNNACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTA
-GAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTT
-AATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTACTGGTTACAGAGAAG
-GCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTA
-GATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGG
-CTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAAT
-TGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCC
-CCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGA
-CGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATG
-GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGT
-GATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAA
-TCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTG
-GTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCA
-TTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAG
-TCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTA
-AAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACT
-GCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGT
-TGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATA
-GTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGT
-GCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTC
-TGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGAC
-AAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAA
-GTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTT
-TTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTA
-ACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCT
-GCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTT
-GCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTG
-CAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGAT
-ACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTAT
-TCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTT
-GTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCA
-GGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGA
-CATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAA
-GAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACA
-CCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTT
-TTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTT
-CCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTT
-GAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTAC
-GCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAG
-CTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACC
-TCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACA
-AGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCT
-CTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAAT
-GTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACC
-TAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTT
-ACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATA
-GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGG
-TAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAG
-CTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTT
-GTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAAT
-TGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTT
-TATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA
-ATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTT
-GTTCTTTTTTTTTTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTG
-TCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATG
-CCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTG
-TGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGT
-GGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCT
-CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTG
-TGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATT
-TTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTT
-TCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACAT
-TAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACAT
-CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACAC
-AATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCA
-GGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTA
-GTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTT
-GTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGA
-AAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTA
-TGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGT
-TGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAA
-TACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTG
-TTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCT
-GCTGTCAAATTACATAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGC
-TTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTAC
-AGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTT
-GTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGT
-ACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTT
-TCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTT
-AAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGG
-TGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGT
-ATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATG
-TGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGG
-GTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACAT
-CTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACA
-ATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTT
-AAGGATTGTCCAGCTGTTGTTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCA
-ACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAG
-AAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGAT
-ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCG
-AAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATAC
-AAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCT
-TTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGA
-AGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATG
-ACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTG
-AGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCA
-GGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTT
-CTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTC
-AAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATT
-AAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGT
-GTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCT
-AACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGA
-TTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATC
-TTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAG
-TTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGG
-TTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATA
-GAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACAC
-CGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACC
-AGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATG
-TTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGT
-CTCTATAGAAATAGAGATGTTGACATAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGAT
-ACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGT
-CAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCAT
-GAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAAT
-CCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTA
-TAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAG
-CTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGA
-ACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGA
-CTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCA
-CATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTT
-AGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTT
-TATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGT
-GATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATT
-TAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAAC
-CTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTAC
-ACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT
-TGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCT
-TATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCT
-ACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGT
-GTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTA
-GAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGT
-ACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAG
-TGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGC
-TAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTC
-GGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAA
-AGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCAC
-AAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAG
-AATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCAT
-ATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCA
-TACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCA
-ACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACC
-TACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCT
-ATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAA
-GAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAA
-TTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATA
-CAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTT
-CCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGT
-CTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTG
-ATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTAT
-AATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCA
-TGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTG
-ACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTT
-AAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGC
-TGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATG
-CCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATT
-GTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACA
-TGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTC
-CATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGC
-AATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTTGATGCTTATAACATGATGATCTCAGC
-TGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAA
-ATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACT
-GTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCT
-TTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTG
-TGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAA
-CCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGC
-CCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATG
-GAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCT
-GAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGC
-TATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTC
-AGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCT
-ATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTT
-ATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACT
-ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCG
-TGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGG
-TGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAA
-CATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTT
-TTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTT
-GATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAA
-ATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGA
-GGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGAC
-AGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAAC
-AAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTA
-TTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTTTTCAGATCCTCAGTTTTACATT
-CAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACT
-AAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGG
-CTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAG
-TCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAANNNNNNNNNNNNNNNNG
-TTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACA
-GGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTA
-TTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACT
-AGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAAAGGAATCTATCAAACTTCT
-AACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAA
-CGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATA
-ATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTAT
-GCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTA
-TAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACNNNCTAATGGTGT
-TGGTTACCAACCATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCAACTGTTTGTGGACCTAAAAAGT
-CTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAAC
-AAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGA
-GATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTG
-TTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTAT
-TCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGA
-CATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTC
-AATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACA
-AATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTG
-TGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAA
-TAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTATAAAACACCACCAATTAAAGAT
-TTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTT
-CAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCA
-TTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCA
-CTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGC
-TTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTG
-CTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCA
-CAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACG
-TCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTC
-AACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAA
-TCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTT
-GCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTC
-CTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACT
-ACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACC
-TGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCT
-CTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCT
-CTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGG
-CTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTT
-GTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAAC
-TTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCA
-GATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCT
-TGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTG
-TTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTC
-TATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATG
-CCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTT
-ACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATT
-GGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCA
-GCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGC
-CTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAA
-CCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGAC
-AGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTG
-CGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTT
-AAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACT
-TTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGT
-AATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTA
-AGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATC
-ACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT
-TGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGA
-CCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTA
-GGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGC
-TTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACC
-ATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTAC
-TATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAA
-TTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACG
-AACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGG
-TACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACA
-AATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCC
-AGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGC
-AATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTG
-CTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATG
-AAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAA
-TGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTA
-TATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGT
-ACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTA
-GTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACT
-AAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACTCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAG
-TAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGT
-TCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGT
-CCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCC
-AAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGG
-TTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTA
-CAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTC
-CTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCA
-ATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAA
-CAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAA
-AGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCA
-GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGC
-ATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTT
-CAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAA
-AGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGAT
-TTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACAC
-AAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTA
-ACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTT
-GAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTA
-TATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNAC
-AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/USA/WY-WYPHL-21025140/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTTAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATTTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACATTTCAAGAAATTCAACTCCAGGCAGCAGTAAACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Paraguay/2152x/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGNNNNNNCTGCAGCTTATT
-ATGTGGGTTATCTTCAACCTAKGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTCTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/Melbourne476/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGATTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTACTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCGGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGATGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAANNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/Melbourne475/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCAC
-TCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACA
-GGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCC
-GATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCG
-ACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAAC
-ATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTG
-AACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTG
-GTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTA
-AGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACT
-TAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCT
-ATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTC
-TAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTA
-AGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACA
-CCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTC
-AACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATC
-CAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTG
-GCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTG
-TTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTG
-CCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTC
-CACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAG
-GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTG
-GTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAAT
-CCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTG
-AACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTAC
-GATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGA
-TGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTG
-TTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCA
-AACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTG
-TCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAAT
-TTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATT
-TAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAG
-AAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAAC
-CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA
-TTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTT
-TTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAAC
-TTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCG
-GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACAT
-ACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTT
-ACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAA
-CTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTC
-AACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAA
-TTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAG
-TGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACC
-TTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAG
-TTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTT
-TAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTAC
-TTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTT
-GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACA
-AACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGA
-GAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAG
-AAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATC
-CAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAA
-AGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATT
-ATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAG
-TGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAA
-CACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTA
-AATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTT
-ACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGT
-ATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAG
-CGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCA
-TCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCC
-ACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAG
-TGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGG
-ACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATG
-ACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTA
-GGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAA
-CTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTG
-GTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGT
-TAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCA
-AAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGA
-TACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTG
-TTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTA
-GTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGG
-ATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGG
-ATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATT
-CTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAA
-CTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATG
-GTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAAT
-TGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGT
-TTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAA
-AACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTA
-ATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTC
-TTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTC
-ATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTT
-TTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAA
-GTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGA
-GTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTA
-AACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTG
-GTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTT
-CTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAG
-AAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTG
-CAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTA
-TGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACA
-TCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAA
-CTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTT
-GCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTA
-TTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACT
-TTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAG
-ACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATG
-GTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGG
-AAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTAC
-CAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGT
-CCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAG
-TTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACAC
-CCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAA
-AAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTG
-CAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAA
-TTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTG
-CTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATA
-CTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTT
-ATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTG
-TCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGT
-ACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATG
-CTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTT
-ATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTA
-GGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGG
-TACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAA
-ATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTA
-TGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTAT
-TCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTT
-ATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATA
-TCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTG
-TAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGT
-TAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTA
-GCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAG
-GTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTG
-GTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATG
-TGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGT
-CTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTA
-TGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCAC
-CATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA
-ATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACA
-TGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAG
-TTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATC
-GATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTC
-TAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATAT
-TGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAG
-GTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTAC
-TCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTG
-CAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTG
-CCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGA
-CATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTG
-CTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTA
-ATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACT
-CAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTT
-TCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTA
-GACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATT
-CACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATG
-TAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCAT
-CTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA
-CTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAG
-CCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATG
-AGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA
-ATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAG
-TTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCAC
-TCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTA
-CAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAG
-ATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGC
-CTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTA
-GTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGT
-TATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTA
-TCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAG
-TGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAA
-CTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAG
-CTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTG
-GTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACT
-TAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACAC
-TTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATC
-AACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAG
-GGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTG
-TTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAA
-GAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACA
-TATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCA
-TTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGA
-TGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTG
-TGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAA
-TGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGT
-TGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATA
-TGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGAC
-ATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTT
-TAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGT
-TGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGT
-GTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCAC
-TACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAG
-TTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTA
-TGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGT
-TGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAG
-ACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACG
-TAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAG
-AGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAA
-ATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCA
-CCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTAT
-GGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACT
-ATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGT
-TTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAA
-CAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTT
-CTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCA
-AGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTT
-TATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGA
-TCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTAC
-ACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCC
-TAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTC
-AAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGC
-TGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACG
-TAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGAC
-TCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTT
-TCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGA
-TAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTAC
-TGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAG
-AGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGT
-CTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT
-TGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGT
-GCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGA
-GTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTC
-TGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC
-ATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGA
-GTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAA
-TTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGA
-CCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA
-TTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCT
-TAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCAC
-GCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTAC
-ACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATA
-TGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATT
-TAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCT
-TTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCC
-TACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGT
-TGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTT
-TAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGC
-TGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGT
-ACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACC
-ACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAG
-AGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAA
-AATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGC
-TTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTA
-TTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCT
-AGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGG
-TGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTG
-TGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGC
-TTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGG
-TGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTA
-TGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAA
-ACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGT
-GTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACAT
-GATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTG
-GAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGG
-ACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGT
-AGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAA
-TAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCC
-AGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTT
-ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACC
-ATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAA
-AACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTT
-CTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGA
-ACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGG
-ACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTC
-TGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGT
-AGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTG
-TAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCT
-TCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATA
-TACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGT
-TATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGC
-AGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCAT
-TATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGA
-GGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTT
-CGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAAT
-TGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAA
-TTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAAT
-CAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAG
-AGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTG
-TTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCT
-CAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCC
-ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCAT
-TTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATG
-TTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACC
-ACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATT
-GCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTA
-AGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCAT
-TGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATA
-GAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGNNNNNNCTGCAGCTTATT
-ATGTGGGTTATCTTCAACCTAKGACTTTTCTATTAAAATATAATGAAAATGGAACCATTA
-CAGATGCTGTAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACCAACAGAATCTA
-TTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATT
-CTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTA
-AATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATG
-AAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTG
-GTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGA
-GAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTT
-TTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTG
-GACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTT
-TAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTG
-GCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTA
-ACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATG
-CAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACAC
-GTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTA
-GTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTG
-CTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAA
-TTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTG
-CTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCA
-AACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATAT
-TACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTG
-CTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTCTGCTCA
-CAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTT
-GGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACC
-AATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTG
-GAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAAC
-TTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGA
-CATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTG
-CTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGG
-GCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAA
-AAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACAC
-AAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACT
-GTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATT
-TAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCC
-TCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGT
-ATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGG
-GCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCA
-AAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCAC
-AATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGG
-CGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATG
-GCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAAC
-AGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCT
-TTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTG
-GCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCAT
-TACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTC
-AGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTAC
-CTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAAT
-CGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGT
-TTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGT
-ATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAA
-TATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTT
-CTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAG
-CTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATT
-TGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGA
-ATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGG
-CTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTC
-AATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCT
-GGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACA
-TCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGT
-TTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGA
-CTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCAT
-TTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGA
-GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGA
-GTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGG
-CAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCA
-ATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCT
-TATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG
-ATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTT
-TTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCA
-TTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGAC
-CCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCA
-CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTG
-GGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGT
-GTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATC
-AGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATA
-CTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAG
-GACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAA
-GAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG
-GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC
-GCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAA
-AAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTA
-GAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACC
-AGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGA
-AATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACC
-AGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCC
-CCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAA
-CGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTA
-AAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAAC
-AGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAAC
-AATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAA
-TGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAAT
-CTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGC
-CACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/Melbourne477/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTTCTTTACATAGAAGTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
\ No newline at end of file
diff --git a/test_data/OTHER/other_sra_metadata.csv b/test_data/OTHER/other_sra_metadata.csv
deleted file mode 100755
index 8c759f1..0000000
--- a/test_data/OTHER/other_sra_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-ncbi-spuid,ncbi-spuid_namespace,ncbi-bioproject,sra-file_location,sra-file_name,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-loader
-seq1,CDC-OAMD,PRJNA512913,local,"fastq_1_R1.fastq.gz, fastq_1_R2.fastq.gz",Other Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-seq2,CDC-OAMD,PRJNA512913,local,"fastq_2_R1.fastq.gz, fastq_2_R2.fastq.gz",Other Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
diff --git a/test_data/POX/pox_biosample_metadata.csv b/test_data/POX/pox_biosample_metadata.csv
deleted file mode 100755
index 0c55739..0000000
--- a/test_data/POX/pox_biosample_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-ncbi-spuid,ncbi-spuid_namespace,bs-description,organism,ncbi-bioproject,bs-isolate,bs-collected_by,bs-package,collection_date,bs-host,bs-host_disease,bs-isolation_source,bs-geo_loc_name,bs-lat_lon,bs-host_sex,bs-host_age
-seq1,CDC-OAMD,CDC Test,Monkeypox virus,PRJNA512913,MPXV/human/USA/GA_2741/2020,Helix,Pathogen.cl.1.0,3/28/2020,Homo sapiens,Monkeypox virus,nasal swab,United States: Georgia,Unknown,Male,28
-seq2,CDC-OAMD,CDC Test,Monkeypox virus,PRJNA512962,MPXV/human/USA/GA_3742/2020,Helix,Pathogen.cl.1.0,4/29/2020,Homo sapiens,Monkeypox virus,nasal swab,United States: Georgia,Unknown,Male,45
diff --git a/test_data/POX/pox_config.yaml b/test_data/POX/pox_config.yaml
deleted file mode 100755
index eb54a0a..0000000
--- a/test_data/POX/pox_config.yaml
+++ /dev/null
@@ -1,35 +0,0 @@
-Submission:
- NCBI:
- Username: username
- Password: password
- BioSample_Package: Pathogen.cl.1.0
- Submission_Position: 1
- Description:
- Title: test_submission
- Comment: This is a test submission
- Organization:
- '@role': owner
- '@type': institute
- '@org_id': 12345
- Name: CDC
- Address:
- Affil: Centers for Disease Control and Prevention
- Div: Respiratory Viruses Branch, Division of Viral Diseases
- Street: 1600 Clifton Rd
- City: Atlanta
- Sub: GA
- Postal_code: 30329
- Country: USA
- Email: email@myemail.com
- Phone: ""
- Submitter:
- '@email': email@myemail.com
- '@alt_email':
- Name:
- First: Jane
- Last: Doe
- GISAID:
- Client-Id: TEST-EA76875B00C3
- Username: username
- Password: password
- Submission_Position: 2
diff --git a/test_data/POX/pox_fastq_1_R1.fastq.gz b/test_data/POX/pox_fastq_1_R1.fastq.gz
deleted file mode 100755
index 4f1c470..0000000
Binary files a/test_data/POX/pox_fastq_1_R1.fastq.gz and /dev/null differ
diff --git a/test_data/POX/pox_fastq_1_R2.fastq.gz b/test_data/POX/pox_fastq_1_R2.fastq.gz
deleted file mode 100755
index 76cb481..0000000
Binary files a/test_data/POX/pox_fastq_1_R2.fastq.gz and /dev/null differ
diff --git a/test_data/POX/pox_fastq_2_R1.fastq.gz b/test_data/POX/pox_fastq_2_R1.fastq.gz
deleted file mode 100755
index 4f1c470..0000000
Binary files a/test_data/POX/pox_fastq_2_R1.fastq.gz and /dev/null differ
diff --git a/test_data/POX/pox_fastq_2_R2.fastq.gz b/test_data/POX/pox_fastq_2_R2.fastq.gz
deleted file mode 100755
index 76cb481..0000000
Binary files a/test_data/POX/pox_fastq_2_R2.fastq.gz and /dev/null differ
diff --git a/test_data/POX/pox_genbank_metadata.csv b/test_data/POX/pox_genbank_metadata.csv
deleted file mode 100755
index 0641ebb..0000000
--- a/test_data/POX/pox_genbank_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-sequence_name,ncbi-spuid,ncbi-spuid_namespace,gb-subm_lab,gb-subm_lab_division,gb-subm_lab_addr,authors,gb-publication_status,gb-publication_title,organism,gb-seq_id,src-isolate,collection_date,src-country,src-host,src-isolation_source,src-serotype,src-BioProject,src-BioSample,cmt-StructuredCommentPrefix,cmt-Assembly Method,cmt-Coverage,cmt-Sequencing Technology,cmt-StructuredCommentSuffix
-seq1,seq1,CDC-OAMD,NIH,NCBI,"10 Center Dr, Bethesda, MD, USA 20895","Doe, John, R.; Doe, Jane;",unpublished,,Monkeypox virus,MPXV/human/USA/GA_2741/2020,MPXV/human/USA/GA_2741/2020,3/28/2020,USA: GA,Homo sapiens,missing,Clade 1,PRJNA512913,SAMN02224951,Assembly-Data,Newbler v. 2.3,100x,Illumina ,Assembly-Data
-seq2,seq2,CDC-OAMD,NIH,NCBI,"10 Center Dr, Bethesda, MD, USA 20895","Doe, John; Doe, Jane;",unpublished,,Monkeypox virus,MPXV/human/USA/GA_3742/2020,MPXV/human/USA/GA_3742/2020,4/29/2020,USA: GA,Homo sapiens,missing,Clade 1,PRJNA512962,SAMN02224986,Assembly-Data,Newbler v. 2.3,100x,Illumina,Assembly-Data
diff --git a/test_data/POX/pox_gisaid_metadata.csv b/test_data/POX/pox_gisaid_metadata.csv
deleted file mode 100755
index 08a3056..0000000
--- a/test_data/POX/pox_gisaid_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-sequence_name,organism,collection_date,authors,gs-virus_name,gs-passage,gs-collection_date,gs-location,gs-add_location,gs-host,gs-add_host_info,gs-sampling_strategy,gs-gender,gs-patient_age,gs-patient_status,gs-specimen,gs-outbreak,gs-last_vaccinated,gs-treatment,gs-seq_technology,gs-assembly_method,gs-coverage,gs-orig_lab,gs-orig_lab_addr,gs-provider_sample_id,gs-subm_lab,gs-subm_lab_addr,gs-subm_sample_id,gs-consortium,gs-comment,gs-comment_type
-seq1,Monkeypox virus,3/28/2020,"Doe, John, R.; Doe, Jane;",MPXV/Anzark/12/2022,Original,3/28/2022,Oceania/Australia/Melbourne,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"32 Data St, Mel, Aus",,,,
-seq2,Monkeypox virus,4/29/2020,"Doe, John; Doe, Jane;",MPXV/Anzark/13/2022,Original,3/28/2022,Oceania/Australia/Melbourne,unknown,Human,,,unknown,unknown,unknown,,,,,Illumina NextSeq 550,,3000x,Bio1 Mel,"16 Info St, Mel, Aus",,Bio2 Mel,"33 Data St, Mel, Aus",,,,
diff --git a/test_data/POX/pox_sequence.fasta b/test_data/POX/pox_sequence.fasta
deleted file mode 100755
index 995e77d..0000000
--- a/test_data/POX/pox_sequence.fasta
+++ /dev/null
@@ -1,4244 +0,0 @@
->hCoV-19/Anzark/12/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAATATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGTGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACANNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNNNAAAAAA
-AAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/13/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAATATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGTGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATNNNNNNNNNAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/14/2022
-CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCAC
-TCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGG
-TTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGT
-CCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTG
-GAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAA
-GGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTAT
-GGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGG
-GCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCC
-GATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACA
-TAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTG
-GCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAA
-CTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTC
-TGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAA
-ATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGT
-AGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCA
-TTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAG
-AAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTA
-GGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGC
-CTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAG
-GTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAA
-GTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAG
-TGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTA
-CAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCA
-GAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGC
-CGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA
-CTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGC
-ACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGG
-TTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTA
-AGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCT
-AAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGA
-AGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAGTTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGT
-TAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAATCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTG
-GTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAA
-TATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAG
-AAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAGGTGCTCT
-GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACC
-AGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTG
-GTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAA
-GAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCAC
-TTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAG
-ACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCA
-GTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACAT
-TGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTG
-CAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAA
-GTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGG
-TGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTG
-GTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTT
-GATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGA
-GATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAA
-TCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGAC
-ATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATA
-TATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAA
-TGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACT
-GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCA
-AGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCT
-GTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTAT
-GGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCT
-TGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGC
-CAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAA
-GAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTAT
-AGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCA
-CCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAAC
-CTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTAC
-TAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTT
-TTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATAC
-CCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACA
-AATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTG
-CACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACAT
-GCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGT
-AGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAAC
-AAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCAT
-GGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT
-GTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACA
-GTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAAT
-TATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTT
-CGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTT
-CAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCT
-TTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACC
-AAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGG
-ACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAG
-AAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAA
-AATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATG
-AATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATA
-GCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTG
-TACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAG
-CATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTG
-AAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTA
-CTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGA
-ACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTA
-GACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGC
-AGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCA
-GCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCA
-GCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAA
-TTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAA
-GGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTC
-TGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGA
-CCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGA
-CTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATT
-AATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTAT
-GTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTG
-ATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCT
-GAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGA
-TGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATA
-ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCAT
-ATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACT
-ACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTA
-ATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTT
-GTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGA
-AATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATG
-CTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATA
-ACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTT
-ACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAG
-CTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTA
-CTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCC
-TAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGAT
-CAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTC
-TGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGC
-ATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTG
-GTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTAC
-TCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACA
-TATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGC
-ATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCT
-GCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAA
-TAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTT
-GTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACC
-TCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTG
-TGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACA
-TGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTC
-AGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAA
-GTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTG
-CTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGAC
-TGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTA
-TGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGT
-ACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATG
-AAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTT
-AGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAG
-ATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGT
-ACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTT
-TTTTTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATA
-AGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGT
-TGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTA
-TGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTA
-TGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATC
-TCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTA
-TTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTT
-GTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAG
-GAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTT
-GGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCT
-TACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATT
-CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGT
-AGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTC
-CATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAA
-AAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGC
-TGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA
-TGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCC
-TTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGA
-TGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTA
-GTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAA
-TTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGA
-TGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGA
-AATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGAC
-ACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAG
-TTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTT
-TTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTG
-TGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATC
-GTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAA
-TACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGT
-TATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGT
-GTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGA
-TAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTG
-ATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGT
-CCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTAC
-TAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTG
-TCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGC
-GTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGG
-TATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGC
-CAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCA
-GAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTT
-AAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCA
-TTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATA
-TTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAA
-CTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATC
-TATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGT
-AATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTT
-CTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTATTATCGTTATAATCTACCAACAATGTGTGATATCA
-GACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTC
-ATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAG
-TTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATG
-CCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAA
-AAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAA
-CATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGC
-CTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTAT
-AGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAAC
-CTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCAC
-TTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGA
-AATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGA
-CGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTT
-ATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGC
-TCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGC
-CGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTT
-ACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGAT
-GAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTT
-TTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA
-GATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTA
-GTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT
-GAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAA
-ATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATT
-TTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTC
-TTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCAC
-CACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAA
-AAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGAC
-ATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAA
-CACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAG
-GGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGC
-TTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATAC
-CTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAAT
-GCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAA
-TGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGG
-GCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGT
-CGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATC
-AGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCG
-TGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTA
-GCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCA
-AACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCA
-TAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAA
-GCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCT
-CAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGAC
-TCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATA
-AGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTT
-ACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTT
-CCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAAT
-GTAGTGTGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGC
-ACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTG
-CCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTT
-ATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGC
-ACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTA
-TTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCA
-TTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGA
-ATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATT
-CTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGA
-TTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCA
-CACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGT
-CTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGT
-GGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAG
-CTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTT
-TTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACA
-AAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAA
-GCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGG
-ACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAA
-ACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGG
-TGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACAT
-TAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTAC
-TTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGA
-ATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTG
-GTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGT
-ACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGA
-TGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAA
-TTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCG
-GGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGC
-AACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTG
-TACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAG
-TGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGA
-TTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAA
-AAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTG
-GCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGT
-TACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATG
-GTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATG
-AGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCT
-TCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGA
-ACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCC
-TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG
-ACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTT
-GATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTT
-TGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAAT
-TTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTT
-TATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTT
-CAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAG
-TGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACANNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAG
-ATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCAT
-CATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCA
-TTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACC
-AGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGT
-ATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCT
-TGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCA
-ACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATT
-TGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTT
-CTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGA
-CATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATC
-AGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGT
-TCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCAT
-TGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCA
-TTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACT
-ATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTC
-AACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTG
-AACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGT
-TTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGT
-GACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCAC
-AAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCG
-GGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTT
-TAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCA
-AAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTA
-AACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAA
-AGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAA
-TTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGA
-GTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGAC
-TTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAG
-GTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAAC
-ACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGA
-CTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTA
-ATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGAT
-CTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGC
-CATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCT
-GCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATT
-TGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTT
-CGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTT
-TCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACT
-TGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTAT
-GCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAA
-AAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTG
-TAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTAT
-ACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTC
-AACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAAC
-ATGTCCAAATTCACACAATCGACGTTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACG
-ACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTT
-AATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGAT
-TGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTG
-AATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTA
-GCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTT
-CCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTT
-TCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGA
-ATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTAC
-GCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGC
-TTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGT
-GACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCG
-TGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTA
-GCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGA
-GATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATT
-TATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAA
-ATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGT
-ACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCAC
-TGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTT
-TCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT
-TATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGC
-CTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTC
-ACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTA
-CAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGT
-AGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATA
-TCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGT
-TCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCT
-GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAA
-TGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTC
-TCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGAC
-CAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTA
-TTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTG
-AGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCT
-CAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACG
-TAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTG
-ATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAA
-GGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCCAAATTTCAAAGATCA
-AGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG
-CTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGAT
-TTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGAT
-GGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAG
-CACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCC
-ACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGA
-GCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTNNNNNNNNNNNNNCAAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/16/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTNNNN
-NNNNNNNNNATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCA
-CTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAG
-AGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACGCACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/17/2022
-CAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTG
-CACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTA
-CGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCT
-TGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCT
-TTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAA
-AAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGT
-TATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATG
-TGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGC
-GCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAA
-ACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCT
-GTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAA
-CAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACG
-TTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTC
-CAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATG
-GGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGA
-TCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTA
-AAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAA
-GTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTAT
-TGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACA
-TAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAG
-AAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCAC
-AAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAG
-TTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCA
-TCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAA
-GGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGG
-CTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTT
-GGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGA
-CGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAA
-TTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGA
-GCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAG
-AGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAG
-TGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCA
-TTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACC
-TAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGA
-TAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGC
-TCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCA
-ACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGT
-CTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAA
-GAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGC
-CACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAAC
-AAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACA
-CCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGA
-CATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTG
-TTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTT
-AAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAA
-AGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAG
-CTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC
-TTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGC
-TGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGA
-AAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATT
-GACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCC
-ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTG
-AAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTAC
-ACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAA
-GCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTG
-TCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGAT
-TATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAAC
-TCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAG
-TGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCT
-GAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGG
-TATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTA
-TCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATT
-AACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGT
-TACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGG
-CTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAA
-TACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCA
-ACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTT
-GTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAA
-CATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGG
-TGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTA
-AACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAG
-CATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAAC
-TTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAA
-ACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGAC
-AATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAG
-CTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTG
-CTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCC
-TCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAA
-ACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAG
-AGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATA
-CAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTT
-AAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTA
-ATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACT
-ATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGT
-TTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTA
-AAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTAT
-TTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAAT
-CTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATT
-TGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCT
-TTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGT
-TGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTT
-TCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATT
-TCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTG
-TAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA
-GAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACA
-TTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTAC
-TGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAA
-AGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCT
-ATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCT
-TATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGT
-TTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAA
-GCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTC
-AGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTA
-ATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGT
-CATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACA
-ACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTG
-TTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACA
-CTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAG
-TGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAAC
-ATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTC
-ATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTT
-CTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACAT
-CAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAAT
-GTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATT
-TCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAA
-GATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTT
-TTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATC
-AGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTT
-TTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTT
-TACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGC
-ACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAA
-AGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAA
-GCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATA
-TAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTT
-GTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATC
-ACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAAC
-TTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAG
-ACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAA
-CTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTA
-TAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAAT
-GTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTAT
-GACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTT
-TTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGT
-TGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCT
-ATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGT
-TTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAG
-AAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAG
-GGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTT
-TTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAAC
-ATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCT
-AGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTAT
-GTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACAC
-TTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATA
-ATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGA
-GTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTA
-CTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACA
-CAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATT
-GTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAG
-TCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGAC
-ATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGC
-TGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCC
-TTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTT
-AAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGAT
-GGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGA
-CAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTT
-CCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTG
-TGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAAC
-TTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTC
-AAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCAC
-TGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATT
-TGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACA
-GACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGG
-TAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTG
-CTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATG
-TTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGC
-ATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTAC
-AAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAA
-GGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGC
-GGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAA
-TGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAA
-TTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGAT
-TGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCT
-TACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATAC
-TTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTA
-CGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGC
-TGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCA
-CGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACT
-GCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAG
-GTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGAT
-GCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAA
-ATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGT
-AAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTA
-ATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCC
-GGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACA
-CTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATA
-TCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAA
-GTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAAT
-GAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGT
-ATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCAT
-CAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCA
-CAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCA
-TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTC
-TATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGG
-AACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG
-CACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTAT
-AGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTC
-TGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTC
-TTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTT
-TGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGG
-GGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATG
-CTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACAT
-GATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGA
-GTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCAT
-TAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAA
-TTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGG
-TATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATA
-AAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTAC
-ATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACT
-GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGAC
-CACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTT
-GAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCT
-GACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACC
-CAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTC
-CAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATAC
-AGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA
-TACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTA
-AATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGT
-CAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTA
-AGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACT
-TGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAA
-ATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAG
-GCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCT
-GTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCAC
-TCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTT
-GCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA
-CAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACA
-CCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAA
-GACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCT
-ATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACC
-TTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATT
-TTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGG
-AATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATG
-GGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGAC
-GTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCG
-TTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAA
-TGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGA
-CTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCT
-GCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGT
-AGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACAC
-ATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGT
-AGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATT
-CCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTG
-AGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTA
-GGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTT
-TAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGG
-CTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTAC
-ACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGC
-TAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCT
-GGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACT
-GAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAA
-TGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCA
-CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACT
-TACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGA
-TGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAG
-GTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGAC
-AGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACT
-TGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAG
-AAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAA
-CCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAG
-TGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAG
-CTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGA
-CAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGG
-TGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTA
-CAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCC
-GTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTT
-TGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAG
-ATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGAC
-ATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATC
-TCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAA
-CGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC
-CCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTC
-AGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGG
-TTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGAT
-TTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG
-AATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGA
-GTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAA
-TTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATT
-TAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTT
-CAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTA
-TTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTG
-CACTTGACCCTCTCTCAGAAACAAAGTGTANNNNNNNNNNNNNCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTT
-AGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCAC
-CAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCG
-CATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGAT
-TCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATT
-ACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACC
-TGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACA
-CCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTA
-CCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTA
-ATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAG
-TTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCT
-TGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTT
-ATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACA
-GGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACC
-CATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCA
-TCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTT
-ACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGA
-TTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTG
-TTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGT
-GGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAA
-AGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTG
-CACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTA
-GCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAG
-GTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTG
-GCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCT
-TTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGA
-CAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAAT
-TAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAA
-AGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGT
-GACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTG
-AAGGTGTCTTTGTTTCAAATGGCACGCACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGAC
-AACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATT
-AGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCA
-TTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATC
-GATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGAT
-TGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGAT
-CCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGG
-ATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTT
-GTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGT
-TTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCA
-ACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTT
-TATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTC
-CAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATA
-GTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGT
-TATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTA
-CTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAG
-AACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACG
-ACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTAC
-GTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTC
-GATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAAT
-CTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATT
-TTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGG
-TTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAA
-TTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGT
-GGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCG
-TACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGAC
-CGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGC
-TGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCA
-GCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCA
-GTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGC
-AGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAA
-ATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATG
-AAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAAC
-AGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTG
-CACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCA
-GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGT
-GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTT
-AGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT
-GTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGT
-TTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAG
-AGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCG
-ATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGT
-TGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATG
-TCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCA
-GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCG
-CTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGAT
-GACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATG
-GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAA
-CTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTT
-CCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATC
-ACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCG
-GTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAA
-CAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATA
-CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAG
-GAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGC
-ATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGA
-TCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGA
-AGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGAT
-GATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCA
-GATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACA
-TAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGA
-GCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGA
-AGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGC
->hCoV-19/x/Melbourne390/2022
-ACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGC
-TTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGC
-TGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGA
-GAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTAC
-GTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAA
-GTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGG
-TCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCC
-CTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGT
-TACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAA
-CACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACA
-ACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTG
-TCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACAC
-GGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGG
-AATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGC
-TTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAA
-GTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATT
-TGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAAT
-TCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCG
-CACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCG
-CTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAA
-AAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGC
-TTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATT
-TTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCA
-TTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTT
-ACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTG
-ATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAAC
-ATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCT
-TAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAA
-AGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATT
-GGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAA
-ATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCA
-CAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAA
-GCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCT
-TGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACA
-CTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAG
-AAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAAC
-TTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTG
-ATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGAT
-TGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT
-TGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTG
-GTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAA
-CTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAA
-TGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAG
-GAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCNNNNNNNNNNNNNNNNNCTAATGGA
-CCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGT
-TAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTAT
-TATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTA
-GCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAA
-GATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATG
-ATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTT
-TATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGA
-TGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCA
-CTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAAT
-GGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAA
-TGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAA
-TGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTG
-GTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAA
-TGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTC
-TCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAA
-ACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACA
-ACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTG
-AAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGAC
-AACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGC
-TGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTG
-TTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAG
-TGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTG
-TTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCT
-TAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGT
-GTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAA
-CTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAA
-AGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACA
-AAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAG
-TTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAA
-CGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGA
-AACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTAC
-ACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCAC
-GTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGAAAAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGA
-AGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCT
-ACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAA
-TAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGA
-AACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGG
-GATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAA
-CCGTGTTTGTACTAATTATATGCCNNNNNNNNNNACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTA
-GAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTT
-AATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTACTGGTTACAGAGAAG
-GCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTA
-GATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGG
-CTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAAT
-TGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCC
-CCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGA
-CGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATG
-GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGT
-GATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAA
-TCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTG
-GTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCA
-TTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAG
-TCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTA
-AAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACT
-GCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGT
-TGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATA
-GTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGT
-GCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTC
-TGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGAC
-AAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAA
-GTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTT
-TTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTA
-ACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCT
-GCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTT
-GCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTG
-CAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGAT
-ACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTAT
-TCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTT
-GTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCA
-GGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGA
-CATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAA
-GAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACA
-CCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTT
-TTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTT
-CCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTT
-GAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTAC
-GCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAG
-CTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACC
-TCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACA
-AGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCT
-CTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAAT
-GTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACC
-TAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTT
-ACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATA
-GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGG
-TAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAG
-CTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTT
-GTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAAT
-TGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTT
-TATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA
-ATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTT
-GTTCTTTTTTTTTTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTG
-TCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATG
-CCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTG
-TGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGT
-GGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCT
-CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTG
-TGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATT
-TTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTT
-TCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACAT
-TAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACAT
-CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACAC
-AATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCA
-GGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTA
-GTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTT
-GTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGA
-AAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTA
-TGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGT
-TGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAA
-TACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTG
-TTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCT
-GCTGTCAAATTACATAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGC
-TTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTAC
-AGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTT
-GTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGT
-ACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTT
-TCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTT
-AAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGG
-TGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGT
-ATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATG
-TGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGG
-GTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACAT
-CTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACA
-ATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTT
-AAGGATTGTCCAGCTGTTGTTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCA
-ACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAG
-AAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGAT
-ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCG
-AAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATAC
-AAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCT
-TTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGA
-AGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATG
-ACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTG
-AGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCA
-GGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTT
-CTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTC
-AAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATT
-AAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGT
-GTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCT
-AACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGA
-TTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATC
-TTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAG
-TTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGG
-TTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATA
-GAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACAC
-CGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACC
-AGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATG
-TTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGT
-CTCTATAGAAATAGAGATGTTGACATAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGAT
-ACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGT
-CAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCAT
-GAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAAT
-CCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTA
-TAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAG
-CTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGA
-ACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGA
-CTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCA
-CATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTT
-AGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTT
-TATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGT
-GATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATT
-TAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAAC
-CTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTAC
-ACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT
-TGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCT
-TATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCT
-ACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGT
-GTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTA
-GAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGT
-ACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAG
-TGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGC
-TAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTC
-GGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAA
-AGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCAC
-AAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAG
-AATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCAT
-ATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCA
-TACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCA
-ACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACC
-TACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCT
-ATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAA
-GAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAA
-TTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATA
-CAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTT
-CCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGT
-CTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTG
-ATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTAT
-AATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCA
-TGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTG
-ACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTT
-AAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGC
-TGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATG
-CCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATT
-GTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACA
-TGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTC
-CATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGC
-AATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTTGATGCTTATAACATGATGATCTCAGC
-TGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAA
-ATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACT
-GTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCT
-TTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTG
-TGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAA
-CCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGC
-CCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATG
-GAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCT
-GAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGC
-TATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTC
-AGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCT
-ATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTT
-ATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACT
-ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCG
-TGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGG
-TGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAA
-CATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTT
-TTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTT
-GATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAA
-ATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGA
-GGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGAC
-AGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAAC
-AAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTA
-TTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGTTTTCAGATCCTCAGTTTTACATT
-CAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACT
-AAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGG
-CTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAG
-TCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAANNNNNNNNNNNNNNNNG
-TTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACA
-GGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTA
-TTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACT
-AGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAAAGGAATCTATCAAACTTCT
-AACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAA
-CGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATA
-ATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTAT
-GCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTA
-TAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACNNNCTAATGGTGT
-TGGTTACCAACCATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCAACTGTTTGTGGACCTAAAAAGT
-CTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAAC
-AAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGA
-GATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTG
-TTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTAT
-TCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGA
-CATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTC
-AATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACA
-AATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTG
-TGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAA
-TAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTATAAAACACCACCAATTAAAGAT
-TTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTT
-CAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCA
-TTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCA
-CTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGC
-TTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTG
-CTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCA
-CAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACG
-TCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTC
-AACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAA
-TCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTT
-GCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTC
-CTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACT
-ACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACC
-TGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCT
-CTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCT
-CTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGG
-CTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTT
-GTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAAC
-TTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCA
-GATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCT
-TGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTG
-TTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTC
-TATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATG
-CCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTT
-ACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATT
-GGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCA
-GCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGC
-CTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAA
-CCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGAC
-AGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTG
-CGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTT
-AAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACT
-TTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGT
-AATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTA
-AGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATC
-ACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT
-TGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGA
-CCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTA
-GGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGC
-TTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACC
-ATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTAC
-TATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAA
-TTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACG
-AACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGG
-TACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACA
-AATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCC
-AGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGC
-AATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTG
-CTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATG
-AAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAA
-TGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTA
-TATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGT
-ACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTA
-GTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACT
-AAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACTCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAG
-TAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGT
-TCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGT
-CCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCC
-AAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGG
-TTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTA
-CAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTC
-CTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCA
-ATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAA
-CAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAA
-AGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCA
-GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGC
-ATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTT
-CAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAA
-AGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGAT
-TTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACAC
-AAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTA
-ACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTT
-GAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTA
-TATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNAC
-AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/USA/WY-WYPHL-21025140/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTTAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATTTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACATTTCAAGAAATTCAACTCCAGGCAGCAGTAAACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Paraguay/2152x/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGNNNNNNCTGCAGCTTATT
-ATGTGGGTTATCTTCAACCTAKGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTCTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/Melbourne476/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGATTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAA
-CAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTACTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTG
-TATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC
-TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCGGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGATGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAANNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAA
-AAAAAAAAAAAAAAAAAAAAAAAAA
->hCoV-19/Anzark/Melbourne475/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCAC
-TCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACA
-GGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCC
-GATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCG
-ACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAAC
-ATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTG
-AACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTG
-GTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTA
-AGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACT
-TAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCT
-ATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTC
-TAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTA
-AGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACA
-CCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTC
-AACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATC
-CAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTG
-GCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTG
-TTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTG
-CCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTC
-CACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAG
-GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTG
-GTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAAT
-CCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTG
-AACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTAC
-GATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGA
-TGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTG
-TTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCA
-AACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTG
-TCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAAT
-TTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATT
-TAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAG
-AAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAAC
-CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA
-TTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTT
-TTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAAC
-TTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCG
-GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACAT
-ACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTT
-ACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAA
-CTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTC
-AACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAA
-TTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAG
-TGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACC
-TTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAG
-TTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTT
-TAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTAC
-TTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTT
-GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACA
-AACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGA
-GAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAG
-AAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATC
-CAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAA
-AGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATT
-ATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAG
-TGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAA
-CACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTA
-AATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTT
-ACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGT
-ATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAG
-CGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCA
-TCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCC
-ACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAG
-TGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGG
-ACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATG
-ACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTA
-GGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAA
-CTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTG
-GTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGT
-TAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCA
-AAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGA
-TACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTG
-TTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTA
-GTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGG
-ATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGG
-ATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATT
-CTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAA
-CTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATG
-GTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAAT
-TGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGT
-TTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAA
-AACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTA
-ATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTC
-TTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTC
-ATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTT
-TTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAA
-GTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGA
-GTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTA
-AACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTG
-GTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTT
-CTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAG
-AAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTG
-CAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTA
-TGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACA
-TCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAA
-CTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTT
-GCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTA
-TTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACT
-TTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAG
-ACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATG
-GTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGG
-AAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTAC
-CAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGT
-CCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAG
-TTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACAC
-CCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAA
-AAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTG
-CAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAA
-TTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTG
-CTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATA
-CTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTT
-ATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTG
-TCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGT
-ACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATG
-CTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTT
-ATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTA
-GGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGG
-TACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAA
-ATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTA
-TGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTAT
-TCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTT
-ATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATA
-TCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTG
-TAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGT
-TAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTA
-GCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAG
-GTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTG
-GTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATG
-TGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGT
-CTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTA
-TGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCAC
-CATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA
-ATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACA
-TGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAG
-TTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATC
-GATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTC
-TAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATAT
-TGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAG
-GTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTAC
-TCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTG
-CAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTG
-CCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGA
-CATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTG
-CTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTA
-ATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACT
-CAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTT
-TCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTA
-GACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATT
-CACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATG
-TAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCAT
-CTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA
-CTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAG
-CCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATG
-AGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA
-ATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAG
-TTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCAC
-TCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTA
-CAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAG
-ATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGC
-CTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTA
-GTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGT
-TATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTA
-TCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAG
-TGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAA
-CTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAG
-CTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTG
-GTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACT
-TAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACAC
-TTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATC
-AACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAG
-GGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTG
-TTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAA
-GAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACA
-TATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCA
-TTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGA
-TGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTG
-TGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAA
-TGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGT
-TGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATA
-TGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGAC
-ATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTT
-TAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGT
-TGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGT
-GTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCAC
-TACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAG
-TTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTA
-TGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGT
-TGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAG
-ACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACG
-TAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAG
-AGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAA
-ATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCA
-CCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTAT
-GGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACT
-ATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGT
-TTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAA
-CAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTT
-CTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCA
-AGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTT
-TATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGA
-TCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTAC
-ACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCC
-TAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTC
-AAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGC
-TGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACG
-TAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGAC
-TCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTT
-TCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGA
-TAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTAC
-TGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAG
-AGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGT
-CTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT
-TGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGT
-GCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGA
-GTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTC
-TGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC
-ATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGA
-GTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAA
-TTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGA
-CCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA
-TTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCT
-TAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCAC
-GCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTAC
-ACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATA
-TGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATT
-TAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCT
-TTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCC
-TACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGT
-TGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTT
-TAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGC
-TGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGT
-ACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACC
-ACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAG
-AGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAA
-AATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGC
-TTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTA
-TTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCT
-AGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGG
-TGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTG
-TGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGC
-TTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGG
-TGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTA
-TGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAA
-ACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGT
-GTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACAT
-GATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTG
-GAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGG
-ACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGT
-AGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAA
-TAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCC
-AGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTT
-ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACC
-ATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAA
-AACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTT
-CTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGA
-ACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGG
-ACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTC
-TGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGT
-AGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTG
-TAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCT
-TCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATA
-TACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGT
-TATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGC
-AGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCAT
-TATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGA
-GGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTT
-CGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAAT
-TGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAA
-TTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAAT
-CAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAG
-AGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTG
-TTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCT
-CAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCC
-ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCAT
-TTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATG
-TTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACC
-ACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATT
-GCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTA
-AGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCAT
-TGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATA
-GAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGNNNNNNCTGCAGCTTATT
-ATGTGGGTTATCTTCAACCTAKGACTTTTCTATTAAAATATAATGAAAATGGAACCATTA
-CAGATGCTGTAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACCAACAGAATCTA
-TTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATT
-CTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTA
-AATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATG
-AAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTG
-GTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGA
-GAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTT
-TTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACC
-AACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTG
-GACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTT
-TAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTG
-GCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTA
-ACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATG
-CAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACAC
-GTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTA
-GTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTG
-CTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAA
-TTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTG
-CTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCA
-AACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATAT
-TACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTG
-CTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTCTGCTCA
-CAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTT
-GGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACC
-AATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTG
-GAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAAC
-TTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGA
-CATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTG
-CTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGG
-GCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAA
-AAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACAC
-AAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACT
-GTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATT
-TAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCC
-TCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGT
-ATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGG
-GCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCA
-AAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCAC
-AATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGG
-CGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATG
-GCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAAC
-AGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCT
-TTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTG
-GCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCAT
-TACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTC
-AGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTAC
-CTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAAT
-CGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGT
-TTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGT
-ATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAA
-TATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTT
-CTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAG
-CTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATT
-TGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGA
-ATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGG
-CTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTC
-AATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCT
-GGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACA
-TCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGT
-TTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGA
-CTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCAT
-TTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGA
-GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGA
-GTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGG
-CAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCA
-ATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCT
-TATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG
-ATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTT
-TTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCA
-TTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGAC
-CCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCA
-CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTG
-GGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGT
-GTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATC
-AGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATA
-CTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAG
-GACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAA
-GAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG
-GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC
-GCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAA
-AAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTA
-GAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACC
-AGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGA
-AATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACC
-AGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCC
-CCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAA
-CGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTA
-AAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAAC
-AGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAAC
-AATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAA
-TGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAAT
-CTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGC
-CACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
->hCoV-19/Anzark/Melbourne477/2022
-ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCA
-CTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG
-GTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTG
-TCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTT
-GGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAA
-AGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTA
-TGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTG
-GGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGC
-CGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC
-ATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGT
-GGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACA
-ACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTT
-CTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCA
-AATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGG
-TAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATC
-ATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAA
-GAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGT
-AGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTG
-CCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATA
-GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAA
-AGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAA
-GTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTT
-ACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATC
-AGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGG
-CCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCT
-ACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGG
-CACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACG
-GTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATT
-AAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGC
-TAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAG
-AAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTG
-TTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATT
-GGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTA
-ATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATA
-GAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTC
-TGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAAC
-CAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCT
-GGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGA
-AGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA
-CTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAA
-GACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACC
-AGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACA
-TTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTT
-GCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAA
-AGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAG
-GTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCT
-GGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTT
-TGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTG
-AGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAA
-ATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGA
-CATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCAT
-ATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAA
-ATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACAC
-TGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGC
-AAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTC
-TGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTA
-TGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTC
-TTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTG
-CCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGA
-AGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTA
-TAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATC
-ACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAA
-CCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTA
-CTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCT
-TTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATA
-CCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAAC
-AAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGT
-GCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA
-TGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTG
-TAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCAGCACCACCTGCTCAGTATGAACTTAAGCA
-TGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTT
-TGTATTGCATAGACGGTGCTTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAC
-AGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAA
-TTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCT
-TCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCT
-TCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTC
-TTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAAC
-CAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAG
-GACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACA
-GAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAA
-AAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAAT
-GAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTAT
-AGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTT
-GTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAA
-GCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTT
-GAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCT
-ACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTG
-AACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTT
-AGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTG
-CAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTC
-AGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTC
-AGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTACTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTA
-ATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGA
-AGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATT
-CTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTG
-ACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAG
-ACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTAT
-TAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTA
-TGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTT
-GATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGC
-TGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAG
-ATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAAT
-AACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCA
-TATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAAC
-TACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTT
-AATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACT
-TGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTG
-AAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACAT
-GCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCAT
-AACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCT
-TACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCA
-GCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGT
-ACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTC
-CTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGA
-TCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTT
-CTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAG
-CATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTT
-GGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTA
-CTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCAC
-ATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAG
-CATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGC
-TGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATA
-ATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGT
-TGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCAC
-CTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTT
-GTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGAC
-ATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACT
-CAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATA
-AGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGT
-GCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGA
-CTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTT
-ATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTG
-TACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTAT
-GAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTT
-TAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAA
-GATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGG
-TACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTT
-TTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACAT
-AAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
-TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGT
-ATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
-ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAAT
-CTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGT
-ATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACT
-TGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACA
-GGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGT
-TGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTC
-TTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
-TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTG
-TAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTT
-CCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAA
-AAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGG
-CTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACA
-ATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCC
-CTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
-ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTT
-AGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAA
-ATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTG
-ATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTG
-AAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGA
-CACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTA
-GTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
-TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTT
-GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCAT
-CGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAA
-ATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGG
-TTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGG
-TGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATG
-ATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
-GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTG
-TCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTA
-CTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTT
-GTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACG
-CGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTG
-GTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACG
-CCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
-AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGT
-TAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGC
-ATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAAT
-ATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAA
-ACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAAT
-CTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGG
-TAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
-TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATC
-AGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGT
-CATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGA
-GTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTAT
-GCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCA
-AAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACA
-ACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
-CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTA
-TAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAA
-CCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCA
-CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAG
-AAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTG
-ACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTT
-TATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
-CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGG
-CCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCT
-TACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGA
-TGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGT
-TTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTA
-AGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATT
-AGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
-TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAA
-AATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACAT
-TTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGT
-CTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCA
-CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGA
-AAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGA
-CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
-ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCA
-GGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAG
-CTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATA
-CCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAA
-TGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCA
-ATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAG
-GGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
-TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAAT
-CAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC
-GTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGT
-AGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTC
-AAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGC
-ATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACA
-AGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
-TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGA
-CTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTAT
-AAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTT
-TACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTT
-TCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAA
-TGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGG
-CACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
-GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTT
-TATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATG
-CACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACT
-ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGC
-ATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAG
-AATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACAT
-TCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
-ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCC
-ACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAG
-TCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGG
-TGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA
-GCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCT
-TTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACAC
-AAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
-AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGG
-GACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGA
-AACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATG
-GTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA
-TTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTA
-CTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATG
-AATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
-GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAG
-TACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTG
-ATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAA
-ATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACC
-GGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTG
-CAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCT
-GTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
-GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTG
-ATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACA
-AAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGT
-GGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTG
-TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGAT
-GGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACAT
-GAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
-TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACG
-AACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCC
-CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAG
-GACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT
-TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTT
-TTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAA
-TTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
-TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATT
-TCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTA
-GTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCA
-AACTTTACTTTCTTTACATAGAAGTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
-GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCA
-TCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTC
-ATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTAC
-CAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGTGGACCTAAAAAGTCTACTAAT
-TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTT
-TCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTG
-ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT
-CAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGG
-TTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCA
-TTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATC
-ATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTAC
-TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT
-CAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTT
-GAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGG
-TTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAG
-TGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCA
-CAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGC
-GGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
-TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGC
-AAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTT
-AAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACA
-AAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTA
-ATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAG
-AGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGA
-CTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
-GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAA
-CACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAG
-ACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATT
-AATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGA
-TCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTG
-CCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCC
-TGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
-TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGT
-TCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTT
-TTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAAC
-TTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTA
-TGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCA
-AAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGT
-GTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
-TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACT
-CAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAA
-CATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGAC
-GACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGT
-TAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGA
-TTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCT
-GAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
-AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTT
-TCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATT
-TTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGG
-AATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTA
-CGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG
-CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTG
-TGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
-GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGT
-AGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAG
-AGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAAT
-TTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAA
-AATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAG
-TACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCA
-CTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
-TTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGT
-TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAG
-CCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGT
-CACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTT
-ACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAG
-TAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGAT
-ATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
-TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTC
-TGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGA
-ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCT
-CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGA
-CCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
-ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACT
-GAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCC
-TCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCAC
-GTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGT
-GATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACA
-AGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACA
-ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGA
-ACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCAT
-GGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATC
-AAGTCATTTTGCTGAATAAGCATATTGACGCATATAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAG
-GCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGA
-TTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGA
-TGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATA
-GCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGC
-CACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAG
-AGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAAT
\ No newline at end of file
diff --git a/test_data/POX/pox_sra_metadata.csv b/test_data/POX/pox_sra_metadata.csv
deleted file mode 100755
index 8c759f1..0000000
--- a/test_data/POX/pox_sra_metadata.csv
+++ /dev/null
@@ -1,3 +0,0 @@
-ncbi-spuid,ncbi-spuid_namespace,ncbi-bioproject,sra-file_location,sra-file_name,sra-library_name,sra-instrument_model,sra-library_strategy,sra-library_source,sra-library_selection,sra-library_layout,sra-library_construction_protocol,sra-loader
-seq1,CDC-OAMD,PRJNA512913,local,"fastq_1_R1.fastq.gz, fastq_1_R2.fastq.gz",Other Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
-seq2,CDC-OAMD,PRJNA512913,local,"fastq_2_R1.fastq.gz, fastq_2_R2.fastq.gz",Other Test,Illumina NovaSeq 6000,AMPLICON,VIRAL RNA,RT-PCR,PAIRED,Helix Hybrid-Capture Test,latf-load
diff --git a/tools.py b/tools.py
index ddc2bf8..ad66dbe 100755
--- a/tools.py
+++ b/tools.py
@@ -133,7 +133,7 @@ def get_metadata(database: List[str], organism: str, metadata_file: str, config_
# Read in metadata file
metadata = file_handler.load_csv(metadata_file)
# Update seqsender base schema to include needed checks
- if "BioSample" in database or "SRA" in database:
+ if "BIOSAMPLE" in database or "SRA" in database:
seqsender_schema.update_columns({"bioproject":{"checks":Check.str_matches(r"^(?!\s*$).+"),"nullable":False,"required":True}})
biosample_schema = sra_schema = genbank_schema = genbank_cmt_schema = genbank_src_schema = gisaid_schema = None
# Import schemas
@@ -235,6 +235,7 @@ def pretty_print_pandera_errors(file: str, error_msgs: List[pandera.errors.Schem
for column_value_msg, indices in duplicate_errors.items():
print(f"Error: {column_value_msg} is duplicated at indices: '{indices}'.", file=sys.stderr)
print("", file=sys.stderr)
+
# Check user credentials information
def check_credentials(config_dict: Dict[str, Any], database: str) -> None:
# Check username
diff --git a/upload_log.py b/upload_log.py
index d960c4a..1e89de4 100755
--- a/upload_log.py
+++ b/upload_log.py
@@ -87,10 +87,13 @@ def validate_submission_status_df(metadata: pd.DataFrame, database: List[str]) -
sys.exit(1)
# Update values of existing submission_status.csv file
-def update_submission_status_csv(submission_dir: str, update_database: str, update_df: pd.DataFrame):
+def update_submission_status_csv(submission_dir: str, update_database: str, update_df: pd.DataFrame) -> None:
+ # Check if there are updates to be made
+ if update_df.empty:
+ print(f"Error: Unable to update 'submission_status.csv' for '{update_database}' at '{submission_dir}'. The log file may be empty.", file=sys.stderr)
# Pop off directory if inside database directory
- if os.path.split(submission_dir)[1] in ["BIOSAMPLE", "SRA", "GENBANK", "GISAID"]:
- submission_dir = os.path.split(submission_dir)[0]
+ if os.path.split(submission_dir)[-1] in ["BIOSAMPLE", "SRA", "GENBANK", "GISAID"]:
+ submission_dir = os.path.dirname(submission_dir)
submission_status_file = os.path.join(submission_dir, "submission_status_report.csv")
file_handler.validate_file(file_type="submission status report", file_path=submission_status_file)
df = file_handler.load_csv(file_path=submission_status_file)
@@ -101,9 +104,6 @@ def update_submission_status_csv(submission_dir: str, update_database: str, upda
sample_column = (SAMPLE_NAME_DATABASE_PREFIX[update_database] + "sample_name")
elif f"{SAMPLE_NAME_DATABASE_PREFIX[update_database]}segment_name" in update_df:
sample_column = (SAMPLE_NAME_DATABASE_PREFIX[update_database] + "segment_name")
- else:
- print(f"Error: Unable to update 'submission_status.csv' for '{update_database}' at '{submission_dir}'.", file=sys.stderr)
- sys.exit(1)
df = df.set_index(sample_column, drop = False)
update_df = update_df.set_index(sample_column)
df.update(update_df)
@@ -175,7 +175,7 @@ def validate_fields_exist(df: pd.DataFrame):
config_file = df["Config_File"].iloc[0]
file_handler.validate_directory(name = "directory", path = submission_dir)
file_handler.validate_file(file_type = "submission_status_report", file_path = submission_status_file)
- file_handler.validate_file(file_type = " config file", file_path = config_file)
+ file_handler.validate_file(file_type = "config file", file_path = config_file)
# Process submission status of existing biosample/sra database submission
def process_biosample_sra(submission_name: str, database: str, organism: str, submission_log_dir: str, submission_dir: str, curr_status: str, config_dict: Dict[str, Any], submission_type: str) -> Tuple[bool, str]: