diff --git a/i2nca/0.3.12/Dockerfile b/i2nca/0.3.12/Dockerfile
new file mode 100644
index 00000000..6b0cfe56
--- /dev/null
+++ b/i2nca/0.3.12/Dockerfile
@@ -0,0 +1,40 @@
+################## BASE IMAGE ######################
+
+FROM python:3.10.8
+
+################## METADATA ######################
+
+LABEL base_image="python:3.10.8"
+LABEL version="1"
+LABEL software="i2nca"
+LABEL software.version="0.3.12"
+LABEL about.summary="A mass spectrometry imaging tool bundle for preprocessing workflows"
+LABEL about.home="https://github.com/cKNUSPeR/i2nca"
+LABEL about.documentation="https://github.com/cKNUSPeR/i2nca"
+LABEL about.license_file="https://github.com/cKNUSPeR/i2nca/blob/main/COPYING"
+LABEL about.license="SPDX:GPL-3.0-only"
+LABEL about.tags="Proteomics"
+
+################## MAINTAINER ######################
+
+MAINTAINER Jannik Witte <jw1217@gmx.de>
+
+################## INSTALLATION ######################
+
+WORKDIR /app
+
+RUN apt-get update && apt-get install -y \
+    libgl1 \
+    git \
+    && apt-get clean \
+    && rm -rf /var/lib/apt/lists/*
+
+RUN pip install --no-cache-dir --upgrade pip && \
+    pip install --no-cache-dir i2nca==0.3.12
+
+RUN groupadd -g 1001 biodockergroup && \
+    useradd -g biodockergroup -u 1001 biodocker
+
+USER biodocker
+
+