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pathway analysis on FDR? #8

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marwa38 opened this issue Sep 26, 2022 · 1 comment
Open

pathway analysis on FDR? #8

marwa38 opened this issue Sep 26, 2022 · 1 comment

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@marwa38
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marwa38 commented Sep 26, 2022

HI guys
It is not an issue it a question regarding enrichmenet analysis.
Do you recommend running pathway analysis without FDR cutt-off so it is independent of DGE genes?

I am a bit new to rnaseq so your comments are helpful.
M

@risserlin
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We recommend pathway analysis on a ranked unthresholded list if possible with your dataset. Please see - https://www.nature.com/articles/s41596-018-0103-9 for the details.

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