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Seq_typing tool produce empty output #22

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Rohit-Satyam opened this issue Sep 8, 2023 · 0 comments
Open

Seq_typing tool produce empty output #22

Rohit-Satyam opened this issue Sep 8, 2023 · 0 comments

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@Rohit-Satyam
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Rohit-Satyam commented Sep 8, 2023

I tried running your tool for serotype identification of my samples. I constructed two blast databases: One from different whole viral genomes (8Kb) and the other using a specific 3Kb region. When I run my 3Kb query regions for classification, I get empty files and the log generated is given below:

==========> seq_typing <==========

Program start: Fri Sep  8 17:15:22 2023

LOGFILE:
/data/redo_P1/results_wgs/run.20230908-171522.log

COMMAND:
/home/subudhak/miniconda3/bin/python3 /home/subudhak/miniconda3/bin/seq_typing.py assembly -b blast_db_wgs/all_wgs.fasta -f query/sample_441_barcode93.fasta -t nucl -s sample -o results_wgs -j 10

PRESENT DIRECTORY:
/data/redo_P1

VERSION:
Version 2.3
f0f2fa5 (f0f2fa5e454274e4b2df28d4bb9bdb50810ec435) - Commit by GitHub, Fri Aug 11 22:22:16 2023) : Merge pull request #20 from B-UMMI/gbs_types
* remote origin
  Fetch URL: https://github.com/B-UMMI/seq_typing.git
  Push  URL: https://github.com/B-UMMI/seq_typing.git
  HEAD branch: master
  Remote branch:
    master tracked
  Local branch configured for 'git pull':
    master merges with remote master
  Local ref configured for 'git push':
    master pushes to master (up to date)




Checking dependencies...
/home/subudhak/miniconda3/bin/blastn
blastn (2.14.0) found
Running: blastn -task blastn -query /data/redo_P1/query/sample_441_barcode93.fasta -db /data/redo_P1/blast_db_wgs/all_wgs.fasta -out /data/redo_P1/results_wgs/blast_out/results.tab -outfmt 7 qseqid qlen sseqid slen qstart qend sstart send evalue length pident nident mismatch gaps -dust no -num_threads 1
"Module Blast" runtime: 0.0h:0.0m:1.17s

Parsing assembly results

TYPEABLE REFERENCES
No references return a type

Types found:

NT

"Module Parse results" runtime: 0.0h:0.0m:0.0s

"seq_typing.py" runtime: 0.0h:0.0m:4.03s


I think the probable reason behind this is not using double quotes in "7 qseqid qlen sseqid slen qstart qend sstart send evalue length pident nident mismatch gaps" because when I do that and remove -o option, I get some blast results.

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